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The aim of the study was to find appropriate non-chlorinated solvent systems for the extraction and separation of pure pinosylvin (PS) and pinosylvin monomethylether (PSMME) from Scots pine/Norway spruce wood residues. The extraction was performed in a pressurized solvent extractor and the purification and isolation of pure substances was done by flash chromatography. More precisely, pure PS was isolated via sequential flash chromatography in two repetitions in the gradient mode of the mobile phase, with cylcohexane (CX) and ethylacetate (EtOAc), beginning with 100% CX and ending up with 100% EtOAc. The extractives were qualitatively and quantitatively analyzed in terms of PS and PSMME contents by gas chromatography-flame ionization detector (GC-FID), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC) and proton nuclear magnetic resonance (1H-NMR) spectroscopy. [ABSTRACT FROM AUTHOR]

The extraction conditions have been evaluated concerning the recovery of specific polyphenols from Scots pine (Pinus sylvestris) knots by means of an accelerated solvent extractor. The composition of the extracts was determined by gas chromatography, gas chromatographymass spectrometry, and high-performance size-exclusion chromatography. The main phenolic compounds are the stilbenes pinosylvin and its monomethyl ether and the lignan nortrachelogenin (NTG), and their amounts vary a lot from sample to sample. The sequential extraction with a nonpolar solvent as a first step for removing the lipophilic compounds was the most efficient approach for the recovery of both pinosylvins and NTG. For food applications, hot water and 85% aqueous ethanol were good solvents for the production of the substances in focus. An industrial knotwood sample from a pulp mill was sequentially extracted with cyclohexane and ethanol/water (95:5) in a large-scale Soxhlet equipment. The results show that the industrial production of pinosylvins and NTG from knots is feasible. [ABSTRACT FROM AUTHOR]

The lipophilic extractives, that is, wood resin, in Populus grandidentata (bigtooth aspen) and Populus tremuloides (quaking aspen) sapwood, heartwood, and knots were analyzed. The amount and composition of the extractives in sapwood were similar in both species. Triglycerides, short-chain fatty acids, and steryl esters were predominant in sapwood samples. The knots of both species contained similar amounts of extractives as the stemwood, but the composition was quite different. Monoglycerides; long-chain fatty acids and alcohols, with 22 to 28 carbon atoms; hydroxy fatty acids; and ferulic acid esters of fatty alcohols were the major components in heartwood and knots, but were minor components in the sapwood. [ABSTRACT FROM AUTHOR]