Search

Your search keyword '"Ono, Tamotsu"' showing total 2 results
Start Over Author "Ono, Tamotsu" Topic klebsiella
2 results on '"Ono, Tamotsu"'

1. Epidemiology of Escherichia coli, Klebsiella species, and Proteus mirabilis strains producing extended-spectrum β-lactamases from clinical samples in the Kinki Region of Japan.

Author

Nakamura T, Komatsu M, Yamasaki K, Fukuda S, Miyamoto Y, Higuchi T, Ono T, Nishio H, Sueyoshi N, Kida K, Satoh K, Toda H, Toyokawa M, Nishi I, Sakamoto M, Akagi M, Nakai I, Kofuku T, Orita T, Wada Y, Zikimoto T, Koike C, Kinoshita S, Hirai I, Takahashi H, Matsuura N, and Yamamoto Y

Subjects
Bacterial Typing Techniques, DNA, Bacterial genetics, Escherichia coli isolation & purification, Escherichia coli Infections genetics, Escherichia coli Infections microbiology, Humans, Japan epidemiology, Klebsiella isolation & purification, Klebsiella Infections genetics, Klebsiella Infections microbiology, Molecular Epidemiology, Proteus Infections genetics, Proteus Infections microbiology, Proteus mirabilis isolation & purification, Escherichia coli genetics, Escherichia coli Infections epidemiology, Klebsiella genetics, Klebsiella Infections epidemiology, Proteus Infections epidemiology, Proteus mirabilis genetics, beta-Lactamases genetics
Abstract

In the present study, nonduplicate, clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiella spp, and Proteus mirabilis were collected during a 10-year period from 2000 to 2009 at several hospitals in the Kinki region, Japan. The detection rate of E coli markedly increased from 0.24% to 7.25%. The detection rate of Klebsiella pneumoniae increased from 0% to 2.44% and that of P mirabilis from 6.97% to 12.85%. The most frequently detected genotypes were the CTX-M9 group for E coli, the CTX-M2 group for K pneumoniae, and the CTX-M2 group for P mirabilis. E coli clone O25:H4-ST131 producing CTX-M-15, which is spreading worldwide, was first detected in 2007. The most common replicon type of E coli was the IncF type, particularly FIB, detected in 466 strains (69.7%). Of the K pneumoniae strains, 47 (55.3%) were of the IncN type; 77 P mirabilis strains (96.3%) were of the IncT type. In the future, the surveillance of various resistant bacteria, mainly ESBL-producing Enterobacteriaceae, should be expanded to prevent their spread.

Published
2012
Full Text
View/download PDF

2. Laboratory surveillance for prospective plasmid-mediated AmpC beta-lactamases in the Kinki region of Japan.

Author

Yamasaki K, Komatsu M, Abe N, Fukuda S, Miyamoto Y, Higuchi T, Ono T, Nishio H, Sueyoshi N, Kida K, Satoh K, Toyokawa M, Nishi I, Sakamoto M, Akagi M, Nakai I, Kofuku T, Orita T, Wada Y, Jikimoto T, Kinoshita S, Miyamoto K, Hirai I, and Yamamoto Y

Subjects
Anti-Bacterial Agents pharmacology, Bacterial Typing Techniques, DNA Fingerprinting, DNA, Bacterial chemistry, DNA, Bacterial genetics, Electrophoresis, Gel, Pulsed-Field, Escherichia coli drug effects, Escherichia coli genetics, Escherichia coli isolation & purification, Humans, Japan, Klebsiella drug effects, Klebsiella genetics, Klebsiella isolation & purification, Microbial Sensitivity Tests, Molecular Epidemiology, Polymerase Chain Reaction, Proteus mirabilis drug effects, Proteus mirabilis genetics, Proteus mirabilis isolation & purification, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, beta-Lactams pharmacology, Bacterial Proteins biosynthesis, Bacterial Proteins genetics, Enterobacteriaceae Infections microbiology, Escherichia coli enzymology, Klebsiella enzymology, Plasmids analysis, Proteus mirabilis enzymology, beta-Lactamases biosynthesis, beta-Lactamases genetics
Abstract

Extended-spectrum beta-lactamases, plasmid-mediated AmpC beta-lactamases (PABLs), and plasmid-mediated metallo-beta-lactamases confer resistance to many beta-lactams. In Japan, although several reports exist on the prevalence of extended-spectrum beta-lactamases and metallo-beta-lactamases, the prevalence and characteristics of PABLs remain unknown. To investigate the production of PABLs, a total of 22,869 strains of 4 enterobacterial species, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis, were collected during six 6-month periods from 17 clinical laboratories in the Kinki region of Japan. PABLs were detected in 29 (0.13%) of 22,869 isolates by the 3-dimensional test, PCR analysis, and DNA sequencing analysis. PABL-positive isolates were detected among isolates from 13 laboratories. Seventeen of 13,995 (0.12%) E. coli isolates, 8 of 5,970 (0.13%) K. pneumoniae isolates, 3 of 1,722 (0.17%) K. oxytoca isolates, and 1 of 1,182 (0.08%) P. mirabilis isolates were positive for PABLs. Of these 29 PABL-positive strains, 20 (69.0%), 6 (20.7%), 2 (6.9%), and 1 (3.4%) carried the genes for CMY-2, DHA-1, CMY-8, and MOX-1 PABLs, respectively. Pattern analysis of randomly amplified polymorphic DNA and pulsed-field gel electrophoretic analysis revealed that the prevalence of CMY-2-producing E. coli strains was not due to epidemic strains and that 3 DHA-1-producing K. pneumoniae strains were identical, suggesting their clonal relatedness. In conclusion, the DHA-1 PABLs were predominantly present in K. pneumoniae strains, but CMY-2 PABLs were predominantly present in E. coli strains. The present findings will provide significant information to assist in preventing the emergence and further spread of PABL-producing bacteria.

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results