47 results on '"Quevedo, João"'
Search Results
2. Haloperidol alters neurotrophic factors and epigenetic parameters in an animal model of schizophrenia induced by ketamine.
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Valvassori SS, da Rosa RT, Dal-Pont GC, Varela RB, Mastella GA, Daminelli T, Fries GR, Quevedo J, and Zugno AI
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- Humans, Rats, Animals, Haloperidol pharmacology, Brain-Derived Neurotrophic Factor genetics, Glial Cell Line-Derived Neurotrophic Factor, Nerve Growth Factor genetics, Disease Models, Animal, Epigenesis, Genetic, Schizophrenia chemically induced, Schizophrenia drug therapy, Schizophrenia genetics, Ketamine toxicity
- Abstract
This study aimed to evaluate Haloperidol's (Hal) effects on the behavioral, neurotrophic factors, and epigenetic parameters in an animal model of schizophrenia (SCZ) induced by ketamine (Ket). Injections of Ket or saline were administered intraperitoneal (once a day) between the 1st and 14th days of the experiment. Water or Hal was administered via gavage between the 8th and 14th experimental days. Thirty minutes after the last injection, the animals were subjected to behavioral analysis. The activity of DNA methyltransferase (DNMT), histone deacetylase (HDAC), and histone acetyltransferase and levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3), and glial-derived neurotrophic factor (GDNF) were evaluated in the frontal cortex, hippocampus, and striatum. Ket increased the covered distance and time spent in the central area of the open field, and Hal did not reverse these behavioral alterations. Significant increases in the DNMT and HDAC activities were detected in the frontal cortex and striatum from rats that received Ket, Hal, or a combination thereof. Besides, Hal per se increased the activity of DNMT and HDAC in the hippocampus of rats. Hal per se or the association of Ket plus Hal decreased BDNF, NGF, NT-3, and GDNF, depending on the brain region and treatment regimen. The administration of Hal can alter the levels of neurotrophic factors and the activity of epigenetic enzymes, which can be a factor in the development of effect collateral in SCZ patients. However, the precise mechanisms involved in these alterations are still unclear., (© 2023 International Society for Developmental Neuroscience.)
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- 2023
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3. Efficacy and adverse effects of ketamine versus electroconvulsive therapy for major depressive disorder: A systematic review and meta-analysis.
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de A Simoes Moreira D, Gauer LE, Teixeira G, Fonseca da Silva AC, Cavalcanti S, and Quevedo J
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- Humans, Myalgia drug therapy, Antidepressive Agents adverse effects, Ketamine adverse effects, Depressive Disorder, Major drug therapy, Electroconvulsive Therapy adverse effects
- Abstract
Background: ECT is considered the fastest and most effective treatment for TRD. Ketamine seems to be an attractive alternative due to its rapid-onset antidepressant effects and impact on suicidal thoughts. This study aimed to compare efficacy and tolerability of ECT and ketamine for different depression outcomes (PROSPERO/CRD42022349220)., Methods: We searched MEDLINE, Web of Science, Embase, PsycINFO, Google Scholar, Cochrane Library and trial registries, which were the ClinicalTrials.gov and the World Health Organization's International Clinical Trials Registry Platform, without restrictions on publication date., Selection Criteria: randomized controlled trials or cohorts comparing ketamine versus ECT in patients with TRD., Results: Eight studies met the inclusion criteria (of 2875 retrieved). Random-effects models comparing ketamine and ECT regarding the following outcomes were conducted: a) reduction of depressive symptoms severity through scales, g = -0.12, p = 0.68; b) response to therapy, RR = 0.89, p = 0.51; c) reported side-effects: dissociative symptoms, RR = 5.41, p = 0.06; nausea, RR = 0.73, p = 0.47; muscle pain, RR = 0.25, p = 0.02; and headache, RR = 0.39, p = 0.08. Influential & subgroup analyses were performed., Limitations: Methodological issues with high risk of bias in some of the source material, reduced number of eligible studies with high in-between heterogeneity and small sample sizes., Conclusion: Our study showed no evidence to support the superiority of ketamine over ECT for severity of depressive symptoms and response to therapy. Regarding side effects, there was a statistically significant decreased risk of muscle pain in patients treated with ketamine compared to ECT., Competing Interests: Conflict of interest JQ received clinical research support from LivaNova; has speaker bureau membership with Myriad Neuroscience, and Abbvie; is consultant for Eurofarma; is stockholder at Instituto de Neurociências Dr. João Quevedo; and receives copyrights from Artmed Editora, Artmed Panamericana, and Elsevier/Academic Press. None of the other authors have conflicts of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2023
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4. Combination of electroconvulsive stimulation with ketamine or escitalopram protects the brain against inflammation and oxidative stress induced by maternal deprivation and is critical for associated behaviors in male and female rats.
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Abelaira HM, Rosa T, de Moura AB, Andrade NM, Martinello NS, Maciel LR, Botelho MEM, Borba LA, Chede BC, Arent CO, Joaquim L, Bonfante S, Danielski LG, Tuon T, Petronilho F, Quevedo J, and Réus GZ
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- Animals, Behavior, Animal, Brain metabolism, Escitalopram, Female, Hippocampus metabolism, Inflammation metabolism, Male, Oxidative Stress, Rats, Rats, Wistar, Ketamine pharmacology, Maternal Deprivation
- Abstract
This study aimed at evaluating the treatment effects with ketamine, electroconvulsive stimulation (ECS), escitalopram, alone or in combination in adult rats of both sexes, subjected to the animal model of maternal deprivation (MD). All groups were subjected to the forced swimming test (FST), splash and open field tests. The prefrontal cortex (PFC), hippocampus and serum were collected to analyze oxidative stress and inflammatory parameters. MD induced depressive-like behavior in the FST test in males and reduced grooming time in male and female rats. The treatments alone or combined reversed depressive and anhedonic behavior in females. In males, all treatments increased grooming time, except for ECS + escitalopram + ketamine. MD increased lipid peroxidation and protein carbonylation, nitrite/nitrate concentration and myeloperoxidase activity in the PFC and hippocampus of males and females. However, the treatment's response was sex dependent. Catalase activity decreased in the PFC of males and the PFC and hippocampus of females, and most treatments were not able to reverse it. MD increased the inflammation biomarkers levels in the PFC and hippocampus of males and females, and most treatments were able to reverse this increase. In all groups, a reduction in the interleukin-10 levels in the PFC and hippocampus of female and male rats was observed. Our study shows different responses between the sexes in the patterns evaluated and reinforces the use of the gender variable as a biological factor in MDD related to early stress and in the response of the therapeutic strategies used., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2022
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5. Sex differences on the behavior and oxidative stress after ketamine treatment in adult rats subjected to early life stress.
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Abelaira HM, Veron DC, de Moura AB, Carlessi AS, Borba LA, Botelho MEM, Andrade NM, Martinello NS, Zabot GC, Joaquim L, Biehl E, Bonfante S, Budni J, Petronilho F, Quevedo J, and Réus GZ
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- Animals, Catalase metabolism, Female, Hippocampus metabolism, Interleukin-6 metabolism, Male, Malondialdehyde metabolism, Motor Activity drug effects, Peroxidase metabolism, Prefrontal Cortex metabolism, Rats, Rats, Wistar, Sex Factors, Superoxide Dismutase metabolism, Behavior, Animal drug effects, Hippocampus drug effects, Ketamine pharmacology, Maternal Deprivation, Oxidative Stress drug effects, Prefrontal Cortex drug effects, Stress, Psychological metabolism
- Abstract
This study aimed to evaluate the effects of ketamine, on behavioral parameters, oxidative stress, and inflammation in the brain of male and female rats submitted to the animal model of maternal deprivation (MD). Wistar rats were deprived of maternal care in the first 10 days of life (three hours daily). As adults, male and female rats were divided: control + saline deprived + saline and deprived + ketamine (15 mg/kg). The behavior was evaluated through the open field and forced swimming tests. Then brain was removed for analysis of oxidative damage, the activity of superoxide dismutase (SOD), catalase (CAT), and myeloperoxidase (MPO) activity, and levels of interleukin-6 (IL-6). MD induced depressive behavior in males and ketamine reversed these changes. MD induced an increase in lipid peroxidation in males and females; ketamine reversed these effects in males. Protein carbonylation was increased in males and females, with ketamine decreasing such effects. The concentration of nitrite/nitrate increased in males and females, whereas ketamine decreased this in the PFC of males. SOD and CAT activities were decreased in male and female deprived groups and deprived groups treated with ketamine. MPO activity and IL-6 levels increased in males subjected to MD and ketamine reversed this effect. The results suggest that stressful events in early life can induce behavioral, neuroimmune changes, and oxidative stress, however, such effects depend on sex and brain area. Ketamine presents anti-inflammatory and antioxidant properties and could be considered an alternative for individuals who are resistant to classical treatments., (Copyright © 2021 Elsevier Inc. All rights reserved.)
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- 2021
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6. Ketamine treatment protects against oxidative damage and the immunological response induced by electroconvulsive therapy.
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Gonçalves CL, Abelaira HM, Rosa T, de Moura AB, Veron DC, Borba LA, Botelho MEM, Goldim MP, Garbossa L, Fileti ME, Petronilho F, Ignácio ZM, Quevedo J, and Réus GZ
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- Animals, Antidepressive Agents administration & dosage, Bupropion administration & dosage, Bupropion pharmacology, Combined Modality Therapy, Depressive Disorder, Major therapy, Electroconvulsive Therapy methods, Fluoxetine administration & dosage, Fluoxetine pharmacology, Ketamine administration & dosage, Male, Neuroprotective Agents administration & dosage, Neuroprotective Agents pharmacology, Rats, Rats, Wistar, Antidepressive Agents pharmacology, Electroconvulsive Therapy adverse effects, Ketamine pharmacology, Oxidative Stress drug effects
- Abstract
Background: Electroconvulsive therapy (ECT) is often recommended for major depressive disorder (MDD) for those who do not respond to the first and second antidepressant trials. A combination of two therapies could improve antidepressant efficacy. Thus, this study aimed to investigate the synergistic effects of ECT combined to antidepressants with a different mechanism of action., Methods: Rats were treated once a day, for five days with ketamine (5 mg/kg), fluoxetine (1 mg/kg), and bupropion (4 mg/kg) alone or in combination with ECT (1 mA; 100 V). After, oxidative damage and antioxidant capacity were assessed in the prefrontal cortex (PFC) and hippocampus, and pro-inflammatory cytokines levels were evaluated in the serum., Results: ECT alone increased lipid peroxidation in the PFC and hippocampus. In the PFC of rats treated with ECT in combination with fluoxetine and bupropion, and in the hippocampus of rats treated with ECT combined with ketamine and bupropion there was a reduction in the lipid peroxidation. The nitrite/nitrate was increased by ECT alone but reverted by combination with ketamine in the hippocampus. Superoxide dismutase (SOD) was increased by ECT and maintained by fluoxetine and bupropion in the PFC. ECT alone increased interleukin-1β (IL-1β) and the administration of ketamine was able to revert this increase showing a neuroprotective effect of this drug when in combination with ECT., Conclusion: The treatment with ECT leads to an increase in oxidative damage and alters the immunological system. The combination with ketamine was able to protect against oxidative damage and the immunological response induced by ECT.
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- 2021
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7. Effects of ketamine on prepubertal Wistar rats: Implications on behavioral parameters for Childhood-Onset Schizophrenia.
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Damazio Pacheco F, Canever L, Antunes Mastella G, Gomes Wessler P, Kunz Godoi A, Hubbe I, da Costa Afonso A, Celso D, Quevedo J, and Ioppi Zugno A
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- Animals, Brain drug effects, Disease Models, Animal, Female, Male, Motor Activity drug effects, Rats, Rats, Wistar, Schizophrenia, Stereotyped Behavior drug effects, Behavior, Animal drug effects, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Prepulse Inhibition drug effects
- Abstract
Early childhood schizophrenia (COS) is a rare condition and has no established animal model to test new treatments. Previous studies have shown that repeated doses of 25 mg/kg ketamine produce schizophrenia-like changes in adult male Wistar rats, but adequate doses of ketamine in animal COS studies are not yet known. Male and female Wistar rats, 23 days old, received an injection of ketamine or intraperitoneal saline (i.p.) for 8 days. The animals underwent different behavioral tests: open field, social interaction, pre-pulse startle inhibition (PPI). Female rats showed behavioral changes at all ketamine doses (5, 15, 25 and 50 mg/kg), in contrast to males that only at 50 mg/kg dose had interrupted PPI and higher stereotypy in the open field test. The present study demonstrated that ketamine at a dose of 50 mg/kg once daily from 23 to 31 days postnatal reproduced changes similar to schizophrenia in pre-pubertal male and female Wistar rats and could be used, with other interventions, in future studies with animals in COS., (Copyright © 2019 ISDN. Published by Elsevier Ltd. All rights reserved.)
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- 2019
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8. Acute treatment with ketamine and chronic treatment with minocycline exert antidepressant-like effects and antioxidant properties in rats subjected different stressful events.
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Maciel AL, Abelaira HM, de Moura AB, de Souza TG, Rosa T, Matos D, Tuon T, Garbossa L, Strassi AP, Fileti ME, Goldim MP, Mathias K, Petronilho F, Quevedo J, and Réus GZ
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- Amitriptyline administration & dosage, Animals, Brain drug effects, Brain metabolism, Chronic Disease, Depressive Disorder, Major drug therapy, Disease Models, Animal, Drug Administration Schedule, Drug Therapy, Combination, Male, Maternal Deprivation, Oxidative Stress drug effects, Oxidative Stress physiology, Rats, Wistar, Stress, Psychological metabolism, Antidepressive Agents administration & dosage, Antioxidants administration & dosage, Ketamine administration & dosage, Minocycline administration & dosage, Stress, Psychological drug therapy
- Abstract
Despite decades of research, the fundamental neurochemical and molecular mechanisms underlying the major depressive disorder (MDD) are still poorly understood, and current antidepressant treatments have limited clinical efficacy. In clinical conditions, the rapprochement between the disease and the corrective actions of drugs in laboratory animals is essential for developing effective therapies. Thus, the aim of this study was to evaluate the antidepressant effects of ketamine (N-metil-d-asparte (NMDA) receptor antagonist), minocycline (tetracycline antibiotic), and amitriptyline (classical antidepressant), on behavior and oxidative stress parameters in animals submitted to the chronic mild stress (CMS) and maternal deprivation protocols. For this aim, male Wistar rats were submitted to maternal deprivation or CMS. To induce maternal deprivation, Wistar rats were deprived of maternal care during the first 10 days of life. To induce CMS, Wistar rats were submitted to the CMS for 40 days. To reverse the effects of stress, treatment was done intraperitoneally with a single dose of ketamine (15 mg/kg), and minocycline (25 mg/kg) and amitriptyline (10 mg/kg) by 20 days. After treatment, the animals were submitted to the forced swimming test and then analyzed oxidative stress parameters in the prefrontal cortex (PFC), hippocampus, amygdala and nucleus accumbens (NAc). Treatment with ketamine, minocycline and amitriptyline were able to exert antidepressant effects in the forced swimming test. However, these antidepressant effects were dependent on the stress model by which the animals were exposed. In certain brain regions some treatment strategies had a pro-oxidant effect. Though, most of the strategies used in this study had antioxidant effects, as reported by a decrease on protein and lipid damage, nitrite/nitrate concentration and myeloperoxidase activity. In addition, an increase in the antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymes activities were also evident after treatments. In conclusion, the antidepressant effects of ketamine and minocycline, in the present study, may be associated, at least in part, with its antioxidant and neuroprotective effects in animals subjected to maternal deprivation or CMS., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2018
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9. Mechanism of synergistic action on behavior, oxidative stress and inflammation following co-treatment with ketamine and different antidepressant classes.
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Réus GZ, Matias BI, Maciel AL, Abelaira HM, Ignácio ZM, de Moura AB, Matos D, Danielski LG, Petronilho F, Carvalho AF, and Quevedo J
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- Animals, Antidepressive Agents administration & dosage, Behavior, Animal, Drug Synergism, Drug Therapy, Combination, Ketamine administration & dosage, Male, Oxidative Stress, Rats, Rats, Wistar, Swimming, Antidepressive Agents classification, Antidepressive Agents pharmacology, Inflammation drug therapy, Ketamine pharmacology
- Abstract
Background: Major depressive disorder (MDD) affects many people in the world. However, around 40% of patients do not respond to any pharmacological drugs. An alternative is to use a combination of different pharmacological groups or the combination of a classical antidepressant with a substance that can potentiate its effect. Thus, this study aimed to investigate the synergistic interactions between different antidepressants, including fluoxetine, quetiapine and lamotrigine in combination with ketamine, a N-methyl-d-aspartate (NMDA) receptor antagonist., Methods: Wistar rats were acutely treated with fluoxetine (1.25mg/kg), quetiapine (5mg/kg), and lamotrigine (5.0mg/kg) alone or in combination with ketamine (5.0mg/kg), and then subjected to behavioral tests. In addition, oxidative damage and antioxidant capacity were assessed in the rat brain, and pro-inflammatory cytokines levels were evaluated in the serum., Results: It was observed a synergistic effect of ketamine in combination with fluoxetine on the immobility time in the forced swimming test, indicating an antidepressant effect. Other antidepressant did not show effects when administrated alone or joint to ketamine. The combination of ketamine with other antidepressants, particularly quetiapine, in some brain regions induced an increase in damage to lipids and proteins. However, the combination of ketamine with fluoxetine increased the antioxidant activity of superoxide dismutase, and decreased oxidative damage, thus suggesting a neuroprotective effect of the combination of these drugs. The combination of ketamine with fluoxetine or lamotrigine reduced pro-inflammatory cytokines levels., Conclusion: In conclusion, ketamine induced antioxidant or pro-antioxidant effects dependent of antidepressant classes or brain area., (Copyright © 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
- Published
- 2017
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10. Ketamine Exhibits Different Neuroanatomical Profile After Mammalian Target of Rapamycin Inhibition in the Prefrontal Cortex: the Role of Inflammation and Oxidative Stress.
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Abelaira HM, Réus GZ, Ignácio ZM, Dos Santos MAB, de Moura AB, Matos D, Demo JP, da Silva JBI, Danielski LG, Petronilho F, Carvalho AF, and Quevedo J
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- Amygdala metabolism, Animals, Antidepressive Agents pharmacology, Male, Prefrontal Cortex metabolism, Rats, Wistar, Signal Transduction drug effects, Antioxidants pharmacology, Encephalitis prevention & control, Ketamine pharmacology, Oxidative Stress drug effects, Prefrontal Cortex drug effects, Sirolimus pharmacology
- Abstract
Studies indicated that mammalian target of rapamycin (mTOR), oxidative stress, and inflammation are involved in the pathophysiology of major depressive disorder (MDD). Ketamine, an N-methyl-D-aspartate (NMDA) receptor antagonist, has been identified as a novel MDD therapy; however, the antidepressant mechanism is not fully understood. In addition, the effects of ketamine after mTOR inhibition have not been fully investigated. In the present study, we examined the behavioral and biochemical effects of ketamine in the prefrontal cortex (PFC), hippocampus, amygdala, and nucleus accumbens after inhibition of mTOR signaling in the PFC. Male adult Wistar rats received pharmacological mTOR inhibitor, rapamycin (0.2 nmol) or vehicle into the PFC and then a single dose of ketamine (15 mg/kg, i.p.). Immobility was assessed in forced swimming tests, and then oxidative stress parameters and inflammatory markers were evaluated in the brain and periphery. mTOR activation in the PFC was essential to ketamine's antidepressant-like effects. Ketamine increased lipid damage in the PFC, hippocampus, and amygdala. Protein carbonyl was elevated in the PFC, amygdala, and NAc after ketamine administration. Ketamine also increased nitrite/nitrate in the PFC, hippocampus, amygdala, and NAc. Myeloperoxidase activity increased in the hippocampus and NAc after ketamine administration. The activities of superoxide dismutase and catalase were reduced after ketamine administration in all brain areas studied. Inhibition of mTOR signaling pathways by rapamycin in the PFC was required to protect against oxidative stress by reducing damage and increasing antioxidant enzymes. Finally, the TNF-α level was increased in serum by ketamine; however, the rapamycin plus treatment group was not able to block this increase. Activation of mTOR in the PFC is involved in the antidepressant-like effects of ketamine; however, the inhibition of this pathway was able to protect certain brain areas against oxidative stress, without affecting inflammation parameters.
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- 2017
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11. Ketamine potentiates oxidative stress and influences behavior and inflammation in response to lipolysaccharide (LPS) exposure in early life.
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Réus GZ, Simões LR, Colpo GD, Scaini G, Oses JP, Generoso JS, Prossin AR, Kaddurah-Daouk R, Quevedo J, and Barichello T
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- Animals, Animals, Newborn, Brain drug effects, Corpus Striatum drug effects, Corpus Striatum metabolism, Cytokines metabolism, Disease Models, Animal, Encephalitis chemically induced, Encephalitis complications, Female, Hippocampus drug effects, Hippocampus metabolism, Lipopolysaccharides, Locomotion drug effects, Male, Prefrontal Cortex drug effects, Prefrontal Cortex metabolism, Rats, Wistar, Schizophrenia chemically induced, Schizophrenia complications, Behavior, Animal drug effects, Brain metabolism, Encephalitis metabolism, Ketamine administration & dosage, Oxidative Stress drug effects, Schizophrenia metabolism
- Abstract
Immune activation (IA) during the early neonatal period is a risk factor for the development of schizophrenia. Lipopolysaccharide (LPS) injected in neonates lead to behavioral and brain changes that persist to adult life. We investigated oxidative stress, levels of cytokines, and the locomotor activity of IA in a schizophrenia animal model in which neonatal male Wistar rats were administered with an injection of LPS (50μg/kg) on postnatal day 3 and different doses of ketamine (5, 15 and 25mg/kg) for 7days during adulthood. Rats LPS-induced did not have locomotor activity alterations. Locomotor activity was elevated in neonatally saline-injected in the higher dose ketamine-treated animals. Carbonyl protein in the prefrontal cortex (PFC), hippocampus and striatum were increased in the LPS- and saline-induced in the ketamine (25mg/kg)-treated animals. Lipid damage occurred in the PFC, striatum and hippocampus in the LPS- and saline-induced in the ketamine (15 and 25mg/kg) -treated animals. In the hippocampus the superoxide dismutase (SOD) was decreased in the LPS- and saline-induced in the ketamine-treated with the dose of 25mg/kg. In the PFC SOD was reduced in the LPS-induced in the ketamine (25mg/kg)-treated animals. Catalase in the PFC and hippocampus was reduced in the LPS- and saline-induced in the ketamine (25mg/kg)-treated animals. Pro- and anti-inflammatory cytokines were lower in the brains of LPS-induced in the higher dose ketamine-treated rats. IA influences the locomotor activity and cytokine levels induced by ketamine, and it has a negative effect in potentiating the oxidative stress by higher doses of ketamine in the brain., (Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.)
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- 2017
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12. Pre-clinical investigation of Diabetes Mellitus as a risk factor for schizophrenia.
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Heylmann ASA, Canever L, Gress K, Gomes ST, Fachim I, Michels C, Stopassoli GC, Mastella GA, Steckert AV, Damiani AP, de Andrade VM, Quevedo J, and Zugno AI
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- Alloxan administration & dosage, Animals, Diabetes Mellitus, Type 1 chemically induced, Disease Models, Animal, Excitatory Amino Acid Antagonists administration & dosage, Ketamine administration & dosage, Male, Rats, Rats, Wistar, Risk Factors, Schizophrenia chemically induced, Alloxan pharmacology, Behavior, Animal drug effects, Brain drug effects, Diabetes Mellitus, Experimental chemically induced, Diabetes Mellitus, Type 1 complications, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Prepulse Inhibition drug effects, Reflex, Startle drug effects, Schizophrenia etiology, Social Behavior
- Abstract
This study investigated the behavioral and biochemical parameters of DM1 as a risk factor in an animal model of schizophrenia (SZ). All groups: 1 Control (saline+saline); 2 Alloxan (alloxan+saline); 3 Ketamine (saline+ketamine); 4 (Alloxan+Ketamine) were fasted for a period of 18h before the subsequent induction of DM via a single intraperitoneal (i.p) injection of alloxan (150mg/kg). From the 4th to the 10th days, the animals were injected i.p with ketamine (25mg/kg) or saline, once a day, to induce a model of SZ and 30min after the last administration were subjected to behavioral testing. After, the animals were decapitated and the brain structures were removed. Ketamine induced hyperactivity and in the social interaction, ketamine, alloxan and the association of alloxan+ketamine increased the latency and decreased the number of contacts between animals. The animals from the ketamine, alloxan and alloxan+ketamine groups showed a prepulse startle reflex (PPI) deficit at the three intensities (65, 70 and 75dB). Ketamine was shown to be capable of increasing the activity of acetylcholinesterase (AChE) in the brain structures. Combination of alloxan+ketamine seems to have an exacerbated effect within the cholinergic system. For lipid peroxidation and protein carbonyls, alloxan+ketamine appear to have intensified lipid and protein damage in the three structures. Ketamine and the combination of ketamine+alloxan induced DNA damage in both frequency and damage index. This research found a relationship between DM1 and SZ., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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13. Effects of ketamine administration on mTOR and reticulum stress signaling pathways in the brain after the infusion of rapamycin into prefrontal cortex.
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Abelaira HM, Réus GZ, Ignácio ZM, Dos Santos MA, de Moura AB, Matos D, Demo JP, da Silva JB, Michels M, Abatti M, Sonai B, Dal Pizzol F, Carvalho AF, and Quevedo J
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- Analysis of Variance, Animals, Drug Administration Routes, Enzyme Inhibitors pharmacology, Male, Rats, Rats, Wistar, Endoplasmic Reticulum Stress drug effects, Excitatory Amino Acid Antagonists pharmacology, Immunosuppressive Agents pharmacology, Ketamine pharmacology, Prefrontal Cortex drug effects, Signal Transduction drug effects, Sirolimus pharmacology, TOR Serine-Threonine Kinases metabolism
- Abstract
Recent studies show that activation of the mTOR signaling pathway is required for the rapid antidepressant actions of glutamate N-methyl-D-aspartate (NMDA) receptor antagonists. A relationship between mTOR kinase and the endoplasmic reticulum (ER) stress pathway, also known as the unfolded protein response (UPR) has been shown. We evaluate the effects of ketamine administration on the mTOR signaling pathway and proteins of UPR in the prefrontal cortex (PFC), hippocampus, amygdala and nucleus accumbens, after the inhibiton of mTOR signaling in the PFC. Male adult Wistar rats received pharmacological mTOR inhibitor, rapamycin (0.2 nmol), or vehicle into the PFC and then a single dose of ketamine (15 mg/kg, i.p.). The immunocontent of mTOR, eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), eukaryotic elongation factor 2 kinase (eEF2K) homologous protein (CHOP), PKR-like ER kinase (PERK) and inositol-requiring enzyme 1 (IRE1) - alpha were determined in the brain. The mTOR levels were reduced in the rapamycin group treated with saline and ketamine in the PFC; p4EBP1 levels were reduced in the rapamycin group treated with ketamine in the PFC and nucleus accumbens; the levels of peEF2K were increased in the PFC in the vehicle group treated with ketamine and reduced in the rapamycin group treated with ketamine. The PERK and IRE1-alpha levels were decreased in the PFC in the rapamycin group treated with ketamine. Our results suggest that mTOR signaling inhibition by rapamycin could be involved, at least in part, with the mechanism of action of ketamine; and the ketamine antidepressant on ER stress pathway could be also mediated by mTOR signaling pathway in certain brain structures., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2017
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14. Effects of ketamine administration on the phosphorylation levels of CREB and TrKB and on oxidative damage after infusion of MEK inhibitor.
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Réus GZ, Abaleira HM, Titus SE, Arent CO, Michels M, da Luz JR, dos Santos MA, Carlessi AS, Matias BI, Bruchchen L, Steckert AV, Ceretta LB, Dal-Pizzol F, and Quevedo J
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- Animals, Brain drug effects, Brain metabolism, Infusions, Intravenous, Male, Oxidative Stress drug effects, Phosphorylation drug effects, Phosphorylation physiology, Rats, Rats, Wistar, Aniline Compounds administration & dosage, Benzamides administration & dosage, Cyclic AMP Response Element-Binding Protein metabolism, Ketamine pharmacology, Mitogen-Activated Protein Kinase Kinases antagonists & inhibitors, Oxidative Stress physiology, Receptor, trkB metabolism
- Abstract
Background: Ketamine, an antagonist of N-methyl-d-aspartate (NMDA) receptors, has presented antidepressant effects in basic and clinical studies. The MAPK kinase (MEK) signaling pathway could be a target for novel antidepressant drugs and an important pathway involved in neuronal plasticity. Thus, this study evaluated the effects of the administration of ketamine on the phosphorylation of TrKB and CREB, and oxidative stress parameters in the prefrontal cortex (PFC), hippocampus, amygdala, and nucleus accumbens (NAc) rats, after the inhibition of MAPK pathway (PD184161)., Methods: Male adult Wistar rats were submitted to a surgical procedure to receive a single dose of a pharmacological inhibitor of MAPK (PD184161) at a dose of (0.1μg/μl) or vehicle. Then, they were divided: 1) vehicle+saline; 2) inhibitor PD184161+saline; 3) vehicle+ketamine 15mg/kg; and 4) inhibitor PD184161+ketamine 15mg/kg., Results: MEK inhibitor and ketamine increased the phosphorylation of the transcription factor cAMP response element-binding protein (pCREB) and neurotrophic factor/tropomyosin related kinase B receptor (pTrKB) in the PFC, and decreased pCREB in the hippocampus. The MEK inhibitor abolished ketamine's effects in the hippocampus. In the amygdala, pCREB was decreased, and pTrKB was increased after MEK inhibitor plus ketamine. Ketamine increased the thiobarbituric acid reactive species (TBARS) in the PFC, hippocampus, amygdala, and NAc; MEK inhibitor antagonized these effects. The carbonyl was increased in the PFC by both ketamine and MEK inhibitor, but inhibitor infusion plus ketamine administration reduced this effect. In the amygdala, MEK inhibitor increased carbonyl., Conclusion: Ketamine's effects on pCREB, pTrKB, and oxidative stress are mediated, at least in part, by a mechanism dependent of MAPK signaling inhibition., (Copyright © 2015 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
- Published
- 2016
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15. A single dose of S-ketamine induces long-term antidepressant effects and decreases oxidative stress in adulthood rats following maternal deprivation.
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Réus GZ, Carlessi AS, Titus SE, Abelaira HM, Ignácio ZM, da Luz JR, Matias BI, Bruchchen L, Florentino D, Vieira A, Petronilho F, and Quevedo J
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- Anhedonia drug effects, Anhedonia physiology, Animals, Brain physiopathology, Catalase metabolism, Depressive Disorder physiopathology, Disease Models, Animal, Lipid Peroxidation drug effects, Lipid Peroxidation physiology, Male, Maternal Deprivation, Oxidative Stress physiology, Rats, Wistar, Stress, Psychological drug therapy, Stress, Psychological physiopathology, Superoxide Dismutase metabolism, Antidepressive Agents administration & dosage, Brain drug effects, Depressive Disorder drug therapy, Ketamine administration & dosage, Oxidative Stress drug effects
- Abstract
Ketamine, an antagonist of N-methyl-d-aspartate receptors, has produced rapid antidepressant effects in patients with depression, as well as in animal models. However, the extent and duration of the antidepressant effect over longer periods of time has not been considered. This study evaluated the effects of single dose of ketamine on behavior and oxidative stress, which is related to depression, in the brains of adult rats subjected to maternal deprivation. Deprived and nondeprived Wistar rats were divided into four groups nondeprived+saline; nondeprived+S-ketamine (15 mg/kg); deprived+saline; deprived+S-ketamine (15 mg/kg). A single dose of ketamine or saline was administrated during the adult phase, and 14 days later depressive-like behavior was assessed. In addition, lipid damage, protein damage, and antioxidant enzyme activities were evaluated in the rat brain. Maternal deprivation induces a depressive-like behavior, as verified by an increase in immobility and anhedonic behavior. However, a single dose of ketamine was able to reverse these alterations, showing long-term antidepressant effects. The brains of maternally deprived rats had an increase in protein oxidative damage and lipid peroxidation, but administration of a single dose of ketamine reversed this damage. The activities of antioxidant enzymes superoxide dismutase and catalase were reduced in the deprived rat brains. However, ketamine was also able to reverse these changes. In conclusion, these findings indicate that a single dose of ketamine is able to induce long-term antidepressant effects and protect against neural damage caused by oxidative stress in adulthood rats following maternal deprivation., (© 2015 Wiley Periodicals, Inc.)
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- 2015
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16. Effects of omega-3 supplementation on interleukin and neurotrophin levels in an animal model of schizophrenia.
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Zugno AI, Canever L, Mastella G, Heylmann AS, Oliveira MB, Steckert AV, Castro AA, dal Pizzol F, Quevedo J, and Gama CS
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- Animals, Brain-Derived Neurotrophic Factor drug effects, Disease Models, Animal, Male, Rats, Rats, Wistar, Brain Chemistry, Brain-Derived Neurotrophic Factor analysis, Dietary Supplements, Fatty Acids, Omega-3 administration & dosage, Interleukins analysis, Ketamine administration & dosage, Schizophrenia prevention & control
- Abstract
New studies suggest that polyunsaturated fatty acids, such as omega-3, may reduce the symptoms of schizophrenia. The present study evaluated the preventive effect of omega-3 on interleukines (IL) and neurotrophin brain-derived neurotrophic factor (BDNF) levels in the brains of young rats subjected to a model of schizophrenia. Treatment was performed over 21 days, starting on the 30th day of rat's life. After 14 days of treatment with omega-3 or vehicle, a concomitant treatment with saline or ketamine (25 mg/kg) was started and maintained until the last day of the experiment. BDNF levels in the rat's prefrontal cortex were decreased at 1 h and 24 h after the last administration of ketamine, whereas the group administered with ketamine and omega-3 showed a decrease in BDNF levels only after 24 h. In contrast, both interventions induced similar responses in levels of IL-1β and IL6. These findings suggest that the similarity of IL-1β and IL6 levels in our experimental groups is due to the mechanism of action of ketamine on the immune system. More studies have to be carried out to explain this pathology. In conclusion, according to previous studies and considering the current study, we could suggest a prophylactic role of omega-3 against the outcome of symptoms associated with schizophrenia.
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- 2015
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17. Maternal deprivation disrupts mitochondrial energy homeostasis in the brain of rats subjected to ketamine-induced schizophrenia.
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Zugno AI, Pacheco FD, Budni J, de Oliveira MB, Canever L, Heylmann AS, Wessler PG, da Rosa Silveira F, Mastella GA, Gonçalves CL, Freitas KV, de Castro AA, Streck EL, and Quevedo J
- Subjects
- Animals, Brain drug effects, Energy Metabolism drug effects, Energy Metabolism physiology, Female, Homeostasis drug effects, Male, Oxidative Stress drug effects, Oxidative Stress physiology, Rats, Rats, Wistar, Schizophrenia chemically induced, Brain metabolism, Homeostasis physiology, Ketamine toxicity, Maternal Deprivation, Mitochondria metabolism, Schizophrenia metabolism
- Abstract
Maternal deprivation (MD) appears to be one of the environmental factors involved in the pathophysiology of schizophrenia. A widely used animal model of the schizophrenia involves the administration of ketamine, a dissociative anesthetic, NMDA receptors noncompetitive antagonist, that induce symptoms such as schizophrenia. To clarify the molecular mechanism of schizophrenia induced by MD, we investigated alterations in energetic metabolism, oxidative stress and neurotrophic factor levels in the brain of rats following MD and/or a single administration of ketamine during adulthood. Male Wistar rats were subjected to MD for 10 days. Additionally, these animals received acute ketamine (5, 15 or 25 mg/kg by intraperitoneal route, i.p.) during adulthood, and 30 min later, they were killed and the prefrontal cortex (PFC), the hippocampus and the striatum were removed for molecular analyses. Ketamine 25 mg/kg and/or MD and Ketamine 15 and 5 mg/kg with MD decreased the creatine kinase (CK) activity in the hippocampus. The enzyme activity of succinate dehydrogenase (SDH) in the Krebs cycle had increased in the striatum following the administration of ketamine 25 mg/kg, MD per se or MD plus ketamine 5 and 15 mg/kg. MD per se or MD combined with ketamine in different doses increased the activity of mitochondrial complexes. The PFC of animals subjected to MD and administered with ketamine 5 mg/kg exhibited increased protein carbonyl content. In the hippocampus, ketamine 15 mg/kg, ketamine 25 mg/kg and MD each increased the carbonyl content. In the striatum, the TBARS levels were increased by the administration of ketamine 25 mg/kg. Finally, in the hippocampus, MD alone or in combination with ketamine reduced the Nerve Growth Factor (NGF) levels; however, the Brain-derived Neurotrophic Factor (BDNF) levels were unaltered. In the present study, we suggest that MD increased the risk of psychotic symptoms in adulthood, altering different parameters of energy and oxidative stress. Our results suggest that adverse experiences occurring early in life may sensitize specific neurocircuits to subsequent stressors, inducing vulnerability, and may help us understand the pathophysiological mechanisms involved in this disorder.
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- 2015
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18. Effect of sepsis on behavioral changes on the ketamine-induced animal model of schizophrenia.
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Comim CM, Silva NC, Patrício JJ, Palmas D, Mendonça BP, Bittencourt MO, Cassol OJ Jr, Barichello T, Zugno AI, Quevedo J, and Dal-Pizzol F
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- Animals, Dose-Response Relationship, Drug, Male, Motor Activity drug effects, Motor Activity physiology, Rats, Rats, Wistar, Sepsis complications, Disease Models, Animal, Interpersonal Relations, Ketamine toxicity, Schizophrenia chemically induced, Schizophrenic Psychology, Sepsis psychology
- Abstract
This study aimed to evaluate the effect of sepsis on behavioral changes on the ketamine-induced animal model of schizophrenia. Male Wistar rats underwent Cecal Ligation and Perporation (CLP) with "basic support" or were sham-operated. After 30 days, the animals were submitted to a model of schizophrenia by injection of Ketamine. The behavior tests were performed after 30 min of the injection of Ketamine or saline. Ketamine in doses of 15 and 25mg/kg increased locomotor activity, latency to first contact in the social interaction and stereotyped behavior. Some changes caused by sepsis may be associated with a predisposition to develop schizophrenia in the animal model., (Copyright © 2015 Elsevier B.V. All rights reserved.)
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- 2015
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19. Omega-3 fatty acids prevent the ketamine-induced increase in acetylcholinesterase activity in an animal model of schizophrenia.
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Zugno AI, Chipindo H, Canever L, Budni J, Alves de Castro A, Bittencourt de Oliveira M, Heylmann AS, Gomes Wessler P, da Rosa Silveira F, Damázio LS, Mastella GA, Kist LW, Bogo MR, Quevedo J, and Gama CS
- Subjects
- Acetylcholinesterase genetics, Animals, Gene Expression Regulation, Enzymologic drug effects, Hippocampus drug effects, Hippocampus enzymology, Male, Neostriatum drug effects, Neostriatum enzymology, Prefrontal Cortex drug effects, Prefrontal Cortex enzymology, Rats, Rats, Wistar, Schizophrenia chemically induced, Acetylcholinesterase metabolism, Excitatory Amino Acid Antagonists pharmacology, Fatty Acids, Omega-3 pharmacology, Ketamine antagonists & inhibitors, Ketamine pharmacology, Schizophrenia enzymology
- Abstract
Aims: Schizophrenia is a debilitating neurodevelopmental disorder that is associated with dysfunction in the cholinergic system. Early prevention is a target of treatment to improve long-term outcomes. Therefore, we evaluated the preventive effects of omega-3 fatty acids on AChE activity in the prefrontal cortex, hippocampus and striatum in an animal model of schizophrenia., Main Methods: Young Wistar rats (30 days old) were initially treated with omega-3 fatty acids or vehicle alone. Animals received ketamine to induce an animal model of schizophrenia or saline plus omega-3 fatty acids or vehicle alone for 7 consecutive days beginning on day 15. A total of 22 days elapsed between the treatment and intervention. Animals were sacrificed, and brain structures were dissected to evaluate AChE activity and gene expression., Key Findings: Our results demonstrate that ketamine increased AChE activity in these three structures, and omega-3 fatty acids plus ketamine showed lower values for the studied parameters, which indicate a partial preventive mechanism of omega-3 fatty acid supplementation. We observed no effect on AChE expression. Together, these results indicate that omega-3 fatty acid supplementation effectively reduced AChE activity in an animal model of schizophrenia in all studied structures. In conclusion, the present study provides evidence that ketamine and omega-3 fatty acids affect the cholinergic system, and this effect may be associated with the physiopathology of schizophrenia. Further studies are required to investigate the mechanisms that are associated with this effect., (Copyright © 2014 Elsevier Inc. All rights reserved.)
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- 2015
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20. Ketamine ameliorates depressive-like behaviors and immune alterations in adult rats following maternal deprivation.
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Réus GZ, Nacif MP, Abelaira HM, Tomaz DB, dos Santos MA, Carlessi AS, da Luz JR, Gonçalves RC, Vuolo F, Dal-Pizzol F, Carvalho AF, and Quevedo J
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- Animals, Cytokines cerebrospinal fluid, Depression immunology, Depression psychology, Female, Interleukin-1 blood, Interleukin-6 blood, Interleukin-6 cerebrospinal fluid, Male, Rats, Wistar, Tumor Necrosis Factor-alpha blood, Antidepressive Agents therapeutic use, Cytokines blood, Depression drug therapy, Ketamine therapeutic use, Maternal Deprivation
- Abstract
A growing body of evidence points toward an association between the glutamatergic system, as well as immune system dysregulation and major depression. So, the present study was aimed at evaluating the behavioral and molecular effects of the ketamine, an antagonist of the N-methyl-D-aspartate (NMDA) receptor of glutamate in maternally deprived adult rats. In deprived rats treated with saline, we observed an increase in the immobility time; however, ketamine treatment reversed this effect, decreasing immobility time. In addition, maternal deprivation induced an increase in cytokines: TNF-α and IL-1 in serum, and in IL-6 in serum and cerebrospinal fluid (CSF). Interestingly, ketamine treatment reduced the levels of all the cytokines in deprived rats. In conclusion, these findings further support a relationship between immune activation and depression. Considering the action of ketamine, this study suggested that antagonists of the NMDA receptor, such as ketamine, could exert their effects by modulation of the immune system., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
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- 2015
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21. Ketamine treatment partly reverses alterations in brain derived- neurotrophic factor, oxidative stress and energy metabolism parameters induced by an animal model of depression.
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Réus GZ, Nacif MP, Abelaira HM, Tomaz DB, dos Santos MA, Carlessi AS, Matias BI, da Luz JR, Steckert AV, Jeremias GC, Scaini G, Morais MO, Streck EL, and Quevedo J
- Subjects
- Analysis of Variance, Animals, Animals, Newborn, Creatine Kinase metabolism, Depression pathology, Disease Models, Animal, Female, Male, Maternal Deprivation, Pregnancy, Rats, Rats, Wistar, Thiobarbituric Acid Reactive Substances, Brain-Derived Neurotrophic Factor metabolism, Depression drug therapy, Energy Metabolism drug effects, Excitatory Amino Acid Antagonists therapeutic use, Ketamine therapeutic use, Oxidative Stress drug effects
- Abstract
Studies have suggested that ketamine, a nonselective NMDA receptor antagonist, could be a new drug in the treatment of major depression, but the way ketamine presents such effects remains to be elucidated. Therefore, the objective of this paper was to evaluate the effects of ketamine treatment on parameters related to depression in the brain of adult rats subjected to an animal model of depression. The animals were divided into: non-deprived + saline; non-deprived + ketamine; deprived + saline; deprived + ketamine. Treatments involving ketamine (15 mg/kg) were administered once a day during 14 days in the animal's adult phase. After treatment, the brain derived-neurotrophic factor (BDNF) levels, oxidative stress and energy metabolism activity were evaluated in brain structures of rats involved in the circuit of depression. In the amygdala, hippocampus and nucleus accumbens (NAc), a reduction in BDNF levels was observed in deprived rats, but the animals treated with ketamine reversed the effects of this animal model only in the amygdala and NAc. In addition to this, the complex I activity, in deprived rats, was diminished in the prefrontal cortex (PFC) and amygdala; in the PFC and hippocampus, the complex II-III was diminished in deprived rats; still the administration of ketamine increased the complex IV activity in the PFC and amygdala of rats submitted to the maternal deprivation. In deprived rats, the creatine kinase activity was reduced in the PFC and amygdala, however the administration of ketamine reversed this decrease in the amygdala. The malondialdehyde (MDA) equivalents were increased in non-deprived rats treated with ketamine in the PFC and NAc. Carbonyl levels in the PFC were diminished in control rats that received saline. Though ketamine treatment reversed this effect in deprived rats in the PFC and hippocampus. Still, in NAc, the carbonyl levels were diminished in deprived rats. The superoxide dismutase (SOD) activity was increased in control rats that received ketamine in the PFC and NAc, and were diminished in deprived rats that received saline or ketamine in the PFC and hippocampus. These findings may help to explain that dysfunctions involving BDNF, oxidative stress and energy metabolism within specific brain areas, may be linked with the pathophysiology of depression, and antidepressant effects of ketamine can be positive, at least partially due to the control of these pathways.
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- 2015
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22. MAPK signaling correlates with the antidepressant effects of ketamine.
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Réus GZ, Vieira FG, Abelaira HM, Michels M, Tomaz DB, dos Santos MA, Carlessi AS, Neotti MV, Matias BI, Luz JR, Dal-Pizzol F, and Quevedo J
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- Aniline Compounds, Animals, Benzamides, Blotting, Western, Brain physiopathology, Brain-Derived Neurotrophic Factor metabolism, Depressive Disorder physiopathology, Extracellular Signal-Regulated MAP Kinases drug effects, Extracellular Signal-Regulated MAP Kinases metabolism, MAP Kinase Signaling System physiology, Neuropsychological Tests, Phosphorylation drug effects, Rats, Wistar, Swimming, p38 Mitogen-Activated Protein Kinases drug effects, p38 Mitogen-Activated Protein Kinases metabolism, Antidepressive Agents pharmacology, Brain drug effects, Depressive Disorder drug therapy, Ketamine pharmacology, MAP Kinase Signaling System drug effects
- Abstract
Studies have pointed to a relationship between MAPK kinase (MEK) signaling and the behavioral effects of antidepressant drugs. So, in the present study we examined the behavioral and molecular effects of ketamine, an antagonist of the N-methyl-d-aspartate receptor (NMDA), which has been shown to have an antidepressant effect after the inhibition of MEK signaling in Wistar rats. Our results showed that acute administration of the MEK inhibitor PD184161, produced depressive-like behavior and stopped antidepressant-like effects of ketamine in the forced swimming test. The phosphorylation of extracellular signal-regulated kinase 1/2 (pERK 1/2) was decreased by PD184161 in the amygdala and nucleus accumbens, and the effects of ketamine on pERK 1/2 in the prefrontal cortex and hippocampus were inhibited by PD184161. The ERK 2 levels were decreased by PD184161 in the nucleus accumbens; and the effects of ketamine were blocked in this brain area. The p38 protein kinase (p38MAPK) and proBDNF were inhibited by PD184161, and the MEK inhibitor prevented the effects of ketamine in the nucleus accumbens. In addition, ketamine increased pro-BDNF levels in the hippocampus. In conclusion, our findings demonstrated that an acute blockade of MAPK signaling lead to depressive-like behavior and stopped the antidepressant response of ketamine, suggesting that the effects of ketamine could be mediated, at least in part, by the regulation of MAPK signaling in these specific brain areas., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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23. Evaluation of acetylcholinesterase activity and behavioural alterations induced by ketamine in an animal model of schizophrenia.
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Zugno AI, Matos MP, Canever L, Fraga DB, De Luca RD, Ghedim FV, Deroza PF, de Oliveira MB, Pacheco FD, Valvassori SS, Volpato AM, Budni J, and Quevedo J
- Subjects
- Animals, Corpus Striatum enzymology, Disease Models, Animal, Hippocampus enzymology, Male, Memory drug effects, Prefrontal Cortex enzymology, Rats, Rats, Wistar, Schizophrenia chemically induced, Acetylcholinesterase metabolism, Brain enzymology, Ketamine toxicity, Motor Activity drug effects, Schizophrenia enzymology
- Abstract
Objective: Cognitive deficits in schizophrenia play a crucial role in its clinical manifestation and seem to be related to changes in the cholinergic system, specifically the action of acetylcholinesterase (AChE). Considering this context, the aim of this study was to evaluate the chronic effects of ketamine in the activity of AChE, as well as in behavioural parameters involving learning and memory., Methods: The ketamine was administered for 7 days. A duration of 24 h after the last injection, the animals were submitted to behavioural tests. The activity of AChE in prefrontal cortex, hippocampus and striatum was measured at different times after the last injection (1, 3, 6 and 24 h)., Results: The results indicate that ketamine did not affect locomotor activity and stereotypical movements. However, a cognitive deficit was observed in these animals by examining their behaviour in inhibitory avoidance. In addition, an increase in AChE activity was observed in all structures analysed 1, 3 and 6 h after the last injection. Differently, serum activity of AChE was similar between groups., Conclusion: Chronic administration of ketamine in an animal model of schizophrenia generates increased AChE levels in different brain tissues of rats that lead to cognitive deficits. Therefore, further studies are needed to elucidate the complex mechanisms associated with schizophrenia.
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- 2014
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24. Ketamine and imipramine in the nucleus accumbens regulate histone deacetylation induced by maternal deprivation and are critical for associated behaviors.
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Réus GZ, Abelaira HM, dos Santos MA, Carlessi AS, Tomaz DB, Neotti MV, Liranço JL, Gubert C, Barth M, Kapczinski F, and Quevedo J
- Subjects
- Acetylation, Amygdala drug effects, Amygdala metabolism, Animals, Behavior, Animal physiology, Hippocampus drug effects, Hippocampus metabolism, Male, Nucleus Accumbens metabolism, Prefrontal Cortex drug effects, Prefrontal Cortex metabolism, Rats, Rats, Wistar, Swimming, Antidepressive Agents pharmacology, Behavior, Animal drug effects, Histones metabolism, Imipramine pharmacology, Ketamine pharmacology, Maternal Deprivation, Nucleus Accumbens drug effects
- Abstract
Studies indicate that histone deacetylation is important for long term changes related to stress and antidepressant treatment. The present study aimed to evaluate the effects of the classic antidepressant imipramine, and of an antagonist of the N-methyl-d-asparte (NMDA) receptor, ketamine, on behavior and histone deacetylase (HDAC) activity in the brains of maternally deprived adult rats. To this aim, deprived and non-deprived (control) male Wistar rats were divided into the following groups: non-deprived+saline; non-deprived+imipramine (30 mg/kg); non-deprived+ketamine (15 mg/kg); deprived+saline; deprived+imipramine (30 mg/kg); and deprived+ketamine (15 mg/kg). The drugs were administrated once a day for 14 days during their adult phase. Their behavior were then assessed using the forced swimming and open field tests. In addition, the HDAC activity was evaluated in the prefrontal cortex, hippocampus, amygdala and nucleus accumbens using the kit ELISA-sandwich test. In deprived rats treated with saline, we observed an increase in the immobility time, but treatments with imipramine and ketamine were able to reverse this alteration, decreasing the immobility time. Also, there was a decrease on number of crossings with imipramine treatment in non-deprived rats, and an increase on number of crossings with ketamine treatment in deprived rats. The HDAC activity did not alter in the prefrontal cortex, hippocampus and amygdala by deprivation or via treatment with imipramine or ketamine. However, in the nucleus accumbens we observed an increase of HDAC activity in the deprived rats, and interestingly, imipramine and ketamine treatments were able to decrease HDAC activity in this brain area. These findings provide a novel insight into the epigenetic regulation of histone deacetylase in the nucleus accumbens caused by imipramine and ketamine, and indicate that molecular events are necessary to reverse specific stress-induced behavior., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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25. Rivastigmine reverses cognitive deficit and acetylcholinesterase activity induced by ketamine in an animal model of schizophrenia.
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Zugno AI, Julião RF, Budni J, Volpato AM, Fraga DB, Pacheco FD, Deroza PF, Luca RD, de Oliveira MB, Heylmann AS, and Quevedo J
- Subjects
- Analysis of Variance, Animals, Avoidance Learning drug effects, Behavior, Animal drug effects, Brain drug effects, Brain enzymology, Electroshock, Male, Memory drug effects, Memory, Short-Term drug effects, Motor Activity drug effects, Rats, Rats, Wistar, Rivastigmine, Schizophrenia enzymology, Schizophrenic Psychology, Acetylcholinesterase metabolism, Cholinesterase Inhibitors pharmacology, Cognition Disorders chemically induced, Cognition Disorders psychology, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Neuroprotective Agents pharmacology, Phenylcarbamates pharmacology, Schizophrenia chemically induced
- Abstract
Schizophrenia is one of the most disabling mental disorders that affects up to 1 % of the population worldwide. Although the causes of this disorder remain unknown, it has been extensively characterized by a broad range of emotional, ideational and cognitive impairments. Studies indicate that schizophrenia affects neurotransmitters such as dopamine, glutamate and acetylcholine. Recent studies suggest that rivastigmine (an acetylcholinesterase inhibitor) is important to improve the cognitive symptoms of schizophrenia. Therefore, the present study evaluated the protective effect of rivastigmine against the ketamine-induced behavioral (hyperlocomotion and cognitive deficit) and biochemical (increase of acetylcholinesterase activity) changes which characterize an animal model of schizophrenia in rats. Our results indicated that rivastigmine was effective to improve the cognitive deficit in different task (immediate memory, long term memory and short term memory) induced by ketamine in rats. Moreover, we observed that rivastigmina reversed the increase of acetylcholinesterase activity induced by ketamine in the cerebral cortex, hippocampus and striatum. However, rivastigmine was not able to prevent the ketamine-induced hyperlocomotion. In conslusion, ours results indicate that cholinergic system might be an important therapeutic target in the physiopathology of schizophrenia, mainly in the cognition, but additional studies should be carried.
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- 2013
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26. Ketamine alters behavior and decreases BDNF levels in the rat brain as a function of time after drug administration.
- Author
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Fraga DB, Réus GZ, Abelaira HM, De Luca RD, Canever L, Pfaffenseller B, Colpo GD, Kapczinski F, Quevedo J, and Zugno AI
- Subjects
- Amygdala drug effects, Amygdala metabolism, Animals, Brain metabolism, Corpus Striatum drug effects, Corpus Striatum metabolism, Disease Models, Animal, Hippocampus drug effects, Hippocampus metabolism, Male, Motor Activity drug effects, Prefrontal Cortex drug effects, Prefrontal Cortex metabolism, Rats, Rats, Wistar, Swimming, Time Factors, Anesthetics, Dissociative administration & dosage, Brain drug effects, Brain-Derived Neurotrophic Factor metabolism, Ketamine administration & dosage
- Abstract
Objective: To evaluate behavioral changes and brain-derived neurotrophic factor (BDNF) levels in rats subjected to ketamine administration (25 mg/kg) for 7 days., Method: Behavioral evaluation was undertaken at 1 and 6 hours after the last injection., Results: We observed hyperlocomotion 1 hour after the last injection and a decrease in locomotion after 6 hours. Immobility time was decreased and climbing time was increased 6 hours after the last injection. BDNF levels were decreased in the prefrontal cortex and amygdala when rats were killed 6 hours after the last injection, compared to the saline group and to rats killed 1 hour after the last injection. BDNF levels in the striatum were decreased in rats killed 6 hours after the last ketamine injection, and BDNF levels in the hippocampus were decreased in the groups that were killed 1 and 6 hours after the last injection., Conclusion: These results suggest that the effects of ketamine on behavior and BDNF levels are related to the time at which they were evaluated after administration of the drug.
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- 2013
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27. Evaluation of behavioral and neurochemical changes induced by ketamine in rats: implications as an animal model of mania.
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Ghedim FV, Fraga Dde B, Deroza PF, Oliveira MB, Valvassori SS, Steckert AV, Budni J, Dal-Pizzol F, Quevedo J, and Zugno AI
- Subjects
- Animals, Antimanic Agents administration & dosage, Disease Models, Animal, Drug Interactions, Lithium administration & dosage, Male, Protein Carbonylation drug effects, Rats, Rats, Wistar, Valproic Acid administration & dosage, Behavior, Animal drug effects, Bipolar Disorder chemically induced, Bipolar Disorder drug therapy, Bipolar Disorder metabolism, Ketamine administration & dosage
- Abstract
Bipolar disorder (BD) is a chronic, prevalent, and highly debilitating psychiatric illness characterized by recurrent manic and depressive episodes. Mood stabilizing agents such as lithium and valproate are two primary drugs used to treat BD. To develop a novel animal model of mania (hallmark of BD), it is important to assess the therapeutic and prophylactic effect of these mood stabilizers on the new candidate target animal model. The present work investigates the therapeutic and prophylactic value of lithium and valproate in a novel preclinical animal model of mania, induced by ketamine. In the prevention protocol, wistar rats were pretreated with lithium (47.5 mg/kg, i.p., twice a day), valproate (200 mg/kg, i.p., twice a day), or saline (i.p., twice a day) for 14 days. Between days 8 and 14, the rats were treated with ketamine (25 mg/kg, i.p.) or saline. In the reversal protocol, rats first received ketamine (25 mg/kg, i.p.) or saline. After, the administration of lithium, valproate, or saline was carried out for seven days. Our results indicated that lithium and valproate reversed and prevented ketamine-induced hyperlocomotion. Moreover, lithium and valproate reversed (prefrontal cortex, hippocampus, and striatum) and prevented (prefrontal cortex, hippocampus, striatum, and amygdala) the increase of the TBARS level induced by ketamine. The protein carbonyl formation, induced by ketamine, was reversed by lithium and valproate in the prefrontal cortex, hippocampus, and striatum, and prevented only in the amygdala. These findings support the notion that the administration of ketamine might be a promising pharmacological animal model of mania, which could play a role in the pathophysiology of BD., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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28. Effect of cigarette smoke exposure in the behavioral changes induced by ketamine.
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Deroza PF, Ghedim FV, Heylmann AS, de Luca RD, Budni J, Souza RP, Quevedo J, and Zugno AI
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- Analysis of Variance, Animals, Disease Models, Animal, Male, Rats, Rats, Wistar, Behavior, Animal drug effects, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Movement Disorders etiology, Smoking
- Published
- 2012
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29. Effects of pregabalin on behavioral alterations induced by ketamine in rats.
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Nunes EA, Canever L, Oliveira Ld, Luca RD, Quevedo J, Zugno A, Peregrino A, Crippa JA, Dursun SM, Baker GB, and Hallak JE
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- Animals, Disease Models, Animal, Dose-Response Relationship, Drug, Male, Pregabalin, Rats, Rats, Wistar, Schizophrenia chemically induced, gamma-Aminobutyric Acid administration & dosage, Antipsychotic Agents administration & dosage, Behavior, Animal drug effects, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Psychoses, Substance-Induced drug therapy, Schizophrenia drug therapy, gamma-Aminobutyric Acid analogs & derivatives
- Abstract
Objective: The aim of this study is to investigate the effects of pregabalin on the behavior of rats under the influence of ketamine, an NMDA receptor antagonist that mimics the symptoms of schizophrenia., Methods: Rats were injected with saline or 25 mg/kg ketamine intraperitoneally. After that, behavior modifications were investigated by the evaluation of stereotypy and hyperlocomotion, after treating rats with pregabalin (at doses of 30 mg/kg or 100 mg/kg) or placebo (saline solution)., Results: The administration of pregabalin reduced ketamine-induced hyperlocomotion. However, neither doses of pregabalin had a significant effect on ketamineinduced stereotypy., Conclusion: This is the first study to investigate the effects of pregabalin using an animal model of psychosis. Furthermore, our results indicate that behavioral changes induced by ketamine in rats can be reversed with the use of pregabalin, suggesting its potential to treat psychotic symptoms.
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- 2012
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30. Ketamine plus imipramine treatment induces antidepressant-like behavior and increases CREB and BDNF protein levels and PKA and PKC phosphorylation in rat brain.
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Réus GZ, Stringari RB, Ribeiro KF, Ferraro AK, Vitto MF, Cesconetto P, Souza CT, and Quevedo J
- Subjects
- Animals, Antidepressive Agents administration & dosage, Antidepressive Agents pharmacology, Antidepressive Agents therapeutic use, Brain metabolism, Depression metabolism, Disease Models, Animal, Drug Evaluation, Preclinical, Drug Synergism, Drug Therapy, Combination, Imipramine administration & dosage, Imipramine pharmacology, Immobility Response, Tonic drug effects, Ketamine administration & dosage, Ketamine pharmacology, Motor Activity drug effects, Phosphorylation, Rats, Rats, Wistar, Brain drug effects, Brain-Derived Neurotrophic Factor metabolism, Cyclic AMP Response Element-Binding Protein metabolism, Cyclic AMP-Dependent Protein Kinases metabolism, Depression drug therapy, Imipramine therapeutic use, Ketamine therapeutic use, Protein Kinase C metabolism
- Abstract
A growing body of evidence has pointed to the N-methyl-d-aspartate (NMDA) receptor antagonists as a potential therapeutic target for the treatment of major depression. The present study investigated the possibility of synergistic interactions between antidepressant imipramine with the uncompetitive NMDA receptor antagonist ketamine. Wistar rats were acutely treated with ketamine (5 and 10mg/kg) and imipramine (10 and 20mg/kg) and then subjected to forced swimming tests. The cAMP response element bindig (CREB) and brain-derived neurotrophic factor (BDNF) protein levels and protein kinase C (PKC) and protein kinase A (PKA) phosphorylation were assessed in the prefrontal cortex, hippocampus and amygdala by imunoblot. Imipramine at the dose of 10mg/kg and ketamine at the dose of 5mg/kg did not have effect on the immobility time; however, the effect of imipramine (10 and 20mg/kg) was enhanced by both doses of ketamine. Ketamine and imipramine alone or in combination at all doses tested did not modify locomotor activity. Combined treatment with ketamine and imipramine produced stronger increases of CREB and BDNF protein levels in the prefrontal cortex, hippocampus and amygdala, and PKA phosphorylation in the hippocampus and amygdala and PKC phosphorylation in prefrontal cortex. The results described indicate that co-administration of antidepressant imipramine with ketamine may induce a more pronounced antidepressant activity than treatment with each antidepressant alone. This finding may be of particular importance in the case of drug-resistant patients and could suggest a method of obtaining significant antidepressant actions whilst limiting side effects., (Copyright © 2011 Elsevier B.V. All rights reserved.)
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- 2011
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31. Behavioral changes and mitochondrial dysfunction in a rat model of schizophrenia induced by ketamine.
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de Oliveira L, Fraga DB, De Luca RD, Canever L, Ghedim FV, Matos MP, Streck EL, Quevedo J, and Zugno AI
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- Animals, Brain drug effects, Brain physiopathology, Disease Models, Animal, Electron Transport Complex I metabolism, Electron Transport Complex II metabolism, Electron Transport Complex III metabolism, Electron Transport Complex IV metabolism, Humans, Male, Rats, Rats, Wistar, Receptors, N-Methyl-D-Aspartate antagonists & inhibitors, Time Factors, Tissue Distribution, Electron Transport drug effects, Ketamine pharmacology, Mitochondria drug effects, Mitochondria enzymology, Receptors, N-Methyl-D-Aspartate metabolism, Schizophrenia physiopathology
- Abstract
Evidence from the literature indicates that mitochondrial dysfunction occurs in schizophrenia and other psychiatric disorders. To produce an animal model that simulates psychotic symptoms analogous to those seen in schizophrenic patients, sub-anesthetic doses of N-methyl-D-aspartate (NMDA) receptor antagonists (such as ketamine) have been used. The aim of this study was to evaluate behavioral changes and mitochondrial dysfunction in rats administered ketamine for 7 consecutive days. Behavioral evaluation was performed using an activity monitor 1, 3 and 6 h after the last injection. The activities of mitochondrial respiratory chain complexes I, II, I-III and IV in multiple brain regions (prefrontal cortex, striatum and hippocampus) were also evaluated. Our results showed that hyperlocomotion occurred in the ketamine group 1 and 3 h after the last injection. Stereotypic movements were elevated only when animals were evaluated 1 h after receiving ketamine. In addition, we found that ketamine administration affects the respiratory chain, altering the activity of respiratory chain complexes in the striatum and hippocampus after 1 h, those in the prefrontal cortex and hippocampus after 3 h and those in the prefrontal cortex and striatum 6 h after the last administration of ketamine. These findings suggest that ketamine alters the behavior of rats and changes the activity of respiratory chain complexes in multiple brain regions at different time points.
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- 2011
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32. Effect of acute administration of ketamine and imipramine on creatine kinase activity in the brain of rats.
- Author
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Assis LC, Rezin GT, Comim CM, Valvassori SS, Jeremias IC, Zugno AI, Quevedo J, and Streck EL
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- Animals, Antidepressive Agents, Tricyclic administration & dosage, Brain enzymology, Depression drug therapy, Dose-Response Relationship, Drug, Male, Rats, Rats, Wistar, Stress, Physiological, Brain drug effects, Creatine Kinase metabolism, Excitatory Amino Acid Antagonists administration & dosage, Imipramine administration & dosage, Ketamine administration & dosage
- Abstract
Objective: Clinical findings suggest that ketamine may be used for the treatment of major depression. The present study aimed to compare behavioral effects and brain Creatine kinase activity in specific brain regions after administration of ketamine and imipramine in rats., Method: Rats were acutely given ketamine or imipramine and antidepressant-like activity was assessed by the forced swimming test; Creatine kinase activity was measured in different regions of the brain., Results: The results showed that ketamine (10 and 15mg/kg) and imipramine (20 and 30mg/kg) reduced immobility time when compared to saline group. We also observed that ketamine (10 and 15mg/kg) and imipramine (20 and 30mg/kg) increased Creatine kinase activity in striatum and cerebral cortex. Ketamine at the highest dose (15mg/kg) and imipramine (20 and 30mg/kg) increased Creatine kinase activity in cerebellum and prefrontal cortex. On the other hand, hippocampus was not affected., Conclusion: Considering that metabolism impairment is probably involved in the pathophysiology of depressive disorders, the modulation of energy metabolism (like increase in Creatine kinase activity) by antidepressants could be an important mechanism of action of these drugs.
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- 2009
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33. Different sub-anesthetic doses of ketamine increase oxidative stress in the brain of rats.
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de Oliveira L, Spiazzi CM, Bortolin T, Canever L, Petronilho F, Mina FG, Dal-Pizzol F, Quevedo J, and Zugno AI
- Subjects
- Animals, Brain drug effects, Brain enzymology, Catalase metabolism, Disease Models, Animal, Dose-Response Relationship, Drug, Lipid Peroxidation drug effects, Lipid Peroxidation physiology, Male, Oxidative Stress drug effects, Rats, Rats, Wistar, Schizophrenia chemically induced, Schizophrenia enzymology, Schizophrenia physiopathology, Superoxide Dismutase metabolism, Anesthetics, Dissociative administration & dosage, Brain metabolism, Ketamine administration & dosage, Oxidative Stress physiology
- Abstract
Schizophrenia is a complex neuropsychiatric disorder in which symptoms can be classified as either positive, such as delusions and hallucinations, or negative, such as blunted affect and social withdrawal. However, the mechanisms underlying this disease are poorly understood. There is evidence that reactive oxygen species (ROS) play an important role in the pathogenesis of many diseases, particularly those which are neurological and psychiatric in nature. Ketamine has been used to induce a schizophrenia-like condition as an animal model in which to study this condition. In the present study we tested the effects of sub-anesthetic doses of ketamine on various parameters of oxidative stress in the brain of rats. Our results indicate that lipid peroxidation and tissue protein oxidation were affected by varying sub-anesthetic doses of ketamine in multiple cerebral structures. Additionally, the activity of the antioxidant enzymes CAT and SOD was measured and was also found to be altered in most of the structures tested. In conclusion, we observe an increase in oxidative damage marked by an increase in lipid peroxidation, oxidative protein damage and a decrease in enzymatic defenses, in an animal model of schizophrenia. Given that oxidative stress could be related to schizophrenia, these findings may explain, at least in part, the mechanisms underlying in this disease.
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- 2009
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34. Acute administration of ketamine reverses the inhibition of mitochondrial respiratory chain induced by chronic mild stress.
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Rezin GT, Gonçalves CL, Daufenbach JF, Fraga DB, Santos PM, Ferreira GK, Hermani FV, Comim CM, Quevedo J, and Streck EL
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- Adrenal Glands drug effects, Adrenal Glands pathology, Animals, Body Weight drug effects, Cerebellum drug effects, Cerebellum enzymology, Cerebral Cortex drug effects, Cerebral Cortex enzymology, Eating drug effects, Electron Transport drug effects, Electron Transport physiology, Electron Transport Complex I metabolism, Electron Transport Complex III metabolism, Electron Transport Complex IV metabolism, Male, Organ Size, Rats, Rats, Wistar, Receptors, N-Methyl-D-Aspartate antagonists & inhibitors, Stress, Psychological pathology, Excitatory Amino Acid Antagonists pharmacology, Ketamine pharmacology, Mitochondria drug effects, Mitochondria enzymology, Stress, Psychological drug therapy, Stress, Psychological enzymology
- Abstract
Modulation and dysfunction of the glutamatergic system seems to be involved in depression. Recently a renewed interest in the glutamatergic system as a treatment option for major depression emerged by the finding that the glutamate N-methyl-D-aspartate (NMDA) antagonist ketamine leads to a rapid improvement of depressive symptoms. Several works support the hypothesis that metabolism impairment is involved in the pathophysiology of depression. We have also recently reported that mitochondrial respiratory chain complexes I, III and IV were inhibited in cerebral cortex and cerebellum of rats after 40 days of chronic mild stress (CMS), which is used as an animal model of depression. Thus, we investigated whether the inhibition of these enzymes may be reversed by acute administration of ketamine (15 mg/kg). We verified that CMS decreased the intake of sweet food and ketamine was not able to reverse such effect. Adrenal gland weight was increased in stressed rats and ketamine reversed this alteration. Control group gained weight after 40 days but stressed group did not gain weight after the same period. Stressed animals gained weight after acute administration of ketamine, when compared to the body weight assessed at the beginning of the experiment. Finally, we verified that complexes I, III and IV were inhibited after CMS in cerebral cortex and cerebellum and acute administration of ketamine reversed this inhibition. Based on the present findings, we hypothesized that CMS induces inhibition of mitochondrial respiratory chain (complexes I, III and IV) and that acute administration of ketamine reverses such effect.
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- 2009
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35. Ketamine treatment reverses behavioral and physiological alterations induced by chronic mild stress in rats.
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Garcia LS, Comim CM, Valvassori SS, Réus GZ, Stertz L, Kapczinski F, Gavioli EC, and Quevedo J
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- Adrenal Glands drug effects, Adrenocorticotropic Hormone blood, Animals, Corticosterone blood, Disease Models, Animal, Drug Administration Schedule, Eating drug effects, Exploratory Behavior drug effects, Food Deprivation, Food Preferences drug effects, Locomotion drug effects, Male, Rats, Rats, Wistar, Restraint, Physical methods, Social Isolation, Stress, Psychological blood, Time Factors, Water Deprivation, Behavior, Animal drug effects, Excitatory Amino Acid Antagonists therapeutic use, Ketamine therapeutic use, Stress, Psychological drug therapy, Stress, Psychological physiopathology
- Abstract
Several studies have supported the idea that ionotropic glutamate N-methyl-d-aspartate receptor (NMDA) is an important player in the etiology of psychopathologies, such as anxiety disorders and major depression. Additionally, studies have shown that ketamine induces antidepressant effects in humans as well as in rodents subjected to animal models of depression. In this context, the present study was aimed to evaluate behavioral and physiological effects of acute and chronic administration of ketamine, a NMDA receptor antagonist, in rats exposed to chronic mild stress (CMS). After 40 days of CMS, rats were treated with ketamine (15 mg/kg) and sweet food consumption, body and adrenal gland weight, corticosterone and adrenocorticotropic (ACTH) hormone levels, and hippocampal BDNF protein levels were assessed. Our findings demonstrated that CMS evoked anhedonia, induced hypertrophy of adrenal gland, impaired gain of body weight and increased corticosterone and ACTH circulating levels in rats. Acute and chronic treatment with ketamine reversed the increase in adrenal gland weight, promoted regain of body weight, and normalized corticosterone and ACTH circulating levels. Repeated, but not acute, administration of ketamine reversed anhedonia-like behavior, although the treatment with ketamine per se increased sweet food consumption in non-stressed rats. Finally, acute and chronic ketamine treatment did not alter hippocampal BDNF protein levels in stressed rats. In conclusion, these findings support the idea of a putative role of NMDA receptors in mood-related symptoms, and rapid and robust effects of ketamine in reverting mainly physiological alterations induced by chronic mild stressful situations in rats.
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- 2009
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36. Chronic administration of ketamine elicits antidepressant-like effects in rats without affecting hippocampal brain-derived neurotrophic factor protein levels.
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Garcia LS, Comim CM, Valvassori SS, Réus GZ, Andreazza AC, Stertz L, Fries GR, Gavioli EC, Kapczinski F, and Quevedo J
- Subjects
- Animals, Antidepressive Agents administration & dosage, Antidepressive Agents adverse effects, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Hippocampus metabolism, Imipramine pharmacology, Ketamine administration & dosage, Ketamine adverse effects, Male, Motor Activity drug effects, Rats, Rats, Wistar, Receptors, N-Methyl-D-Aspartate antagonists & inhibitors, Antidepressive Agents pharmacology, Behavior, Animal drug effects, Brain-Derived Neurotrophic Factor metabolism, Hippocampus drug effects, Ketamine pharmacology
- Abstract
A growing body of evidence has pointed to the blockade of the N-methyl-d-aspartate (NMDA) receptor signaling as a potential therapeutic target for the treatment of major depression. The present study was aimed to evaluate behavioural and molecular effects of the chronic treatment with ketamine and imipramine in rats. To this aim, rats were 14 days treated once a day with ketamine (5, 10 and 15 mg/kg) and imipramine (10, 20 and 30 mg/kg) and then subjected to the forced swimming and open-field tests. Ketamine and imipramine, at the all doses tested, reduced immobility time, and increased both climbing and swimming time of rats compared to the saline group, without affecting spontaneous locomotor activity. Brain-derived neurotrophic factor (BDNF) hippocampal levels were assessed in imipramine- and ketamine-treated rats by ELISA sandwich assay. Chronic administration of both drugs, ketamine and imipramine, did not modify BDNF protein levels in the rat hippocampus. In conclusion, our findings demonstrate for the first time that chronic administration of acute inactive doses of ketamine (5 mg/kg) becomes active after chronic treatment, while no signs of tolerance to the behavioural effects of ketamine were observed after chronic administration of acute active doses (10 and 15 mg/kg). Finally, these findings further support the hypothesis that NMDA receptor could be a new pharmacological target for the treatment of mood disorders.
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- 2008
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37. Acute administration of ketamine induces antidepressant-like effects in the forced swimming test and increases BDNF levels in the rat hippocampus.
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Garcia LS, Comim CM, Valvassori SS, Réus GZ, Barbosa LM, Andreazza AC, Stertz L, Fries GR, Gavioli EC, Kapczinski F, and Quevedo J
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- Analysis of Variance, Animals, Dose-Response Relationship, Drug, Imipramine pharmacology, Immobility Response, Tonic drug effects, Male, Rats, Rats, Wistar, Swimming, Antidepressive Agents administration & dosage, Behavior, Animal drug effects, Brain-Derived Neurotrophic Factor metabolism, Hippocampus drug effects, Ketamine administration & dosage
- Abstract
Ketamine is a non-competitive antagonist to the phencyclidine site of N-methyl-d-aspartate (NMDA) receptor. Clinical findings point to a rapid onset of action for ketamine on the treatment of major depression. Considering that classic antidepressants may take long-lasting time to exhibit their main therapeutic effects, the present study aims to compare the behavioral effects and the BDNF hippocampus levels of acute administration of ketamine and imipramine in rats. To this aim, rats were acutely treated with ketamine (5, 10 and 15 mg/kg) and imipramine (10, 20 and 30 mg/kg) and animal behavioral was assessed in the forced swimming and open-field tests. Afterwards, BDNF protein hippocampal levels were assessed in imipramine- and ketamine-treated rats by ELISA-sandwich assay. We observed that ketamine at the doses of 10 and 15 mg/kg, and imipramine at 20 and 30 mg/kg reduced immobility time compared to saline group, without affecting locomotor activity. Interesting enough, acute administration of ketamine at the higher dose, but not imipramine, increased BDNF protein levels in the rat hippocampus. In conclusion, our findings suggest that the increase of hippocampal BDNF protein levels induced by ketamine might be necessary to produce a rapid onset of antidepressant action.
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- 2008
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38. New Perspective on mTOR Pathways: A New Target of Depression
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Réus, Gislaine Z., Ignácio, Zuleide M., Abelaira, Helena M., Quevedo, João, and Kim, Yong-Ku, editor
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- 2018
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39. Effects of omega-3 supplementation on interleukin and neurotrophin levels in an animal model of schizophrenia
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Zugno, Alexandra Ioppi, Canever, Lara, Chipindo, Helder, L., Mastella, Gustavo Antunes, Heylmann, Alexandra S., Oliveira, Mariana B., Steckert, Amanda Valnier, Castro, Adalberto A., Dal Pizzol, Felipe, Quevedo, João Luciano de, and Gama, Clarissa Severino
- Subjects
Omega-3 ,Ácidos graxos ômega-3 ,Ketamina ,Interleukins ,Schizophrenia ,Esquizofrenia ,Ketamine ,Neurotrophins - Abstract
New studies suggest that polyunsaturated fatty acids, such as omega-3, may reduce the symptoms of schizophrenia. The present study evaluated the preventive effect of omega-3 on interleukines (IL) and neurotrophin brain-derived neurotrophic factor (BDNF) levels in the brains of young rats subjected to a model of schizophrenia. Treatment was performed over 21 days, starting on the 30th day of rat’s life. After 14 days of treatment with omega-3 or vehicle, a concomitant treatment with saline or ketamine (25 mg/kg) was started and maintained until the last day of the experiment. BDNF levels in the rat’s prefrontal cortex were decreased at 1 h and 24 h after the last administration of ketamine, whereas the group administered with ketamine and omega-3 showed a decrease in BDNF levels only after 24 h. In contrast, both interventions induced similar responses in levels of IL-1β and IL6. These findings suggest that the similarity of IL-1β and IL6 levels in our experimental groups is due to the mechanism of action of ketamine on the immune system. More studies have to be carried out to explain this pathology. In conclusion, according to previous studies and considering the current study, we could suggest a prophylactic role of omega-3 against the outcome of symptoms associated with schizophrenia.
- Published
- 2015
40. New perspectives on the involvement of mTOR in depression as well as in the action of antidepressant drugs.
- Author
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Ignácio, Zuleide M., Réus, Gislaine Z., Arent, Camila O., Abelaira, Helena M., Pitcher, Meagan R., and Quevedo, João
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MONOAMINE oxidase ,MENTAL depression ,PATHOLOGICAL psychology ,ANTIDEPRESSANTS ,RAPAMYCIN - Abstract
Despite the revolution in recent decades regarding monoamine involvement in the management of major depressive disorder (MDD), the biological mechanisms underlying this psychiatric disorder are still poorly understood. Currently available treatments require long time courses to establish antidepressant response and a significant percentage of people are refractory to single drug or combination drug treatment. These issues, and recent findings demonstrating the involvement of synaptic plasticity in the pathophysiological mechanisms of MDD, are encouraging researchers to explore the molecular mechanisms underlying psychiatric disease in more depth. The discovery of the rapid antidepressant effect exerted by glutamatergic and cholinergic agents highlights the mammalian target of rapamycin (mTOR) pathway as a critical pathway that contributes to the efficacy of these pharmacological agents in clinical and pre-clinical research. The mTOR pathway is a downstream intracellular signal that transmits information after the direct activation of α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) and neurotrophic factor receptors. Activation of these receptors is hypothesized to be one of the major axes involved in the synthesis of synaptogenic proteins underlying synaptic plasticity and critical to both the rapid and delayed effects exerted by classic antidepressants. This review focuses on the involvement of mTOR in the pathophysiology of depression and on molecular mechanisms involved in the activity of emerging and classic antidepressant agents. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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41. Effect of chronic administration of ketamine on the mitochondrial respiratory chain activity caused by chronic mild stress Rezin et al.
- Author
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Rezin, Gislaine T., Gonçalves, Cinara L., Daufenbach, Juliana F., Carvalho-Silva, Milena, Borges, Lislaine S., Vieira, Julia S., Hermani, Fernanda V., Comim, Clarissa M., Quevedo, João, and Streck, Emilio L.
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BRAIN ,KETAMINE ,CEREBRAL cortex ,CEREBELLUM ,LABORATORY rats - Abstract
Rezin GT, Gonçalves CL, Daufenbach JF, Carvalho-Silva M, Borges LS, Vieira JS, Hermani FV, Comim CM, Quevedo J, Streck EL. Effect of chronic administration of ketamine on the mitochondrial respiratory chain activity caused by chronic mild stress. Recently, we reported that mitochondrial respiratory chain complexes I, III and IV were inhibited in the cerebral cortex and cerebellum of rats submitted to chronic mild stress (CMS) and that acute ketamine administration reversed this effect. Therefore, we investigated whether the inhibition of these enzymes may be reversed by chronic administration of ketamine. Adult male Wistar rats were submitted to CMS and chronically treated with ketamine. After 40 days of CMS, consumption of sweet food, adrenal gland weight, body weight and enzymatic activity of the complexes were measured. We verified that CMS decreased the intake of sweet food, increased the adrenal gland weight and the control group gained weight after 40 days but the stressed group did not; ketamine administration reversed these effects. We also verified that chronic administration of ketamine reversed the inhibition of complexes I, III and IV in cerebral cortex. However, in cerebellum, only complex IV inhibition was reversed. The chronic ketamine administration partially reverses the inhibition caused by CMS. We hypothesise that CMS inhibits complexes I, III and IV activities and that chronic administration of ketamine administration partially reverses such an effect. Therefore, it seems reasonable to propose that ketamine administration might be a useful therapy for patients affected by major depression. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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42. The inhibition of the kynurenine pathway prevents behavioral disturbances and oxidative stress in the brain of adult rats subjected to an animal model of schizophrenia.
- Author
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Réus, Gislaine Z., Zugno, Alexandra I., Quevedo, João, Becker, Indianara R.T., Dal-Pizzol, Felipe, Barichello, Tatiana, Scaini, Giselli, Petronilho, Fabricia, Oses, Jean P., Kaddurah-Daouk, Rima, and Ceretta, Luciane B.
- Subjects
- *
KYNURENINE , *OXIDATIVE stress , *BRAIN function localization , *SCHIZOPHRENIA , *ANIMAL behavior - Abstract
Evidence has shown that the kynurenine pathway (KP) plays a role in the onset of oxidative stress and also in the pathophysiology of schizophrenia. The aim of this study was to use a pharmacological animal model of schizophrenia induced by ketamine to investigate if KP inhibitors could protect the brains of Wistar rats against oxidative stress and behavioral changes. Ketamine, injected at the dose of 25 mg/kg, increased spontaneous locomotor activity. However, the inhibitors of tryptophan 2,3-dioxygenase (TDO), indoleamine 2,3-dioxygenase (IDO) and kynurenine-3-monooxygenase (KMO) were able to reverse these changes. In addition, the IDO inhibitor prevented lipid peroxidation, and decreased the levels of protein carbonyl in the prefrontal cortex (PFC), hippocampus and striatum. It also increased the activity of superoxide dismutase (SOD) in the hippocampus, as well as increasing the levels of catalase activity in the PFC and hippocampus. The TDO inhibitor prevented lipid damage in the striatum and reduced the levels of protein carbonyl in the hippocampus and striatum. Also, the TDO inhibitor increased the levels of SOD activity in the striatum and CAT activity in the hippocampus of ketamine-induced pro-oxidant effects. Lipid damage was not reversed by the KMO inhibitor. The KMO inhibitor increased the levels of SOD activity in the hippocampus, and reduced the levels of protein carbonyl while elevating the levels of CAT activity in the striatum of rats that had been injected with ketamine. Our findings revealed that the KP pathway could be a potential mechanism by which a schizophrenia animal model induced by ketamine could cause interference by producing behavioral disturbance and inducing oxidative stress in the brain, suggesting that the inhibition of the KP pathway could be a potential target in treating schizophrenia. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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43. Effects of maternal folic acid supplementation on nuclear methyltransferase activity of adult rats subjected to an animal model of schizophrenia.
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Canever, Lara, Varela, Roger, Mastella, Gustavo A., Damázio, Louyse S., Valvassori, Samira S., Quevedo, João L., and Zugno, Alexandra I.
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- *
FOLIC acid , *LABORATORY rats , *METHYLATION , *ANIMAL disease models , *SCHIZOPHRENIA - Abstract
Introduction: Schizophrenia is considered one of the most disabling and severe human diseases worldwide. The etiology of schizophrenia is thought to be multifactorial and evidence suggests that DNA methylation can play an important role in underlying pivotal neurobiological alterations of this disorder. Some studies have demonstrated the effects of dietary supplementation as an alternative approach to the prevention of schizophrenia, including folic acid. However, no study has ever investigated the role of such supplementation in altering the DNA methylation system in the context of schizophrenia. Objectives: The present study aims to investigate the effects of maternal folic acid supplementation at different doses on nuclear methyltransferase activity of adult rat offspring subjected to an animal model schizophrenia induced by ketamine. Methods: Adult female Wistar rats, (60 days old) received folic acid‐deficient diet, control diet, or control diet plus folic acid supplementation (at 5, 10, or 50 mg/kg) during pregnancy and lactation. After reaching adulthood (60 days), the male offspring of these dams were subjected to the animal model of schizophrenia induced by 7 days of ketamine intraperitoneal injection (25 mg/kg). After the 7‐day protocol, the activity of nuclear methyltransferase was evaluated in the brains of the offspring. Results: Maternal folic acid supplementation at 50 mg/kg increased methyltransferase activity in the frontal cortex, while 10 mg/kg increased methyltransferase activity in the hippocampus. In the striatum of offspring treated with ketamine, maternal deficient diet, control diet, and folic acid supplementation at 5 mg/kg decreased methyltransferase activity compared to the control group. The folic acid supplementation at 10 and 50 mg/kg reversed this ketamine effect. Conclusions: Maternal FA deficiency could be related to schizophrenia pathophysiology, while FA supplementation could present a protective effect since it demonstrated persistent effects in epigenetic parameters in adult offspring. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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44. Effect of folic acid on oxidative stress and behavioral changes in the animal model of schizophrenia induced by ketamine.
- Author
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Zugno, Alexandra I., Canever, Lara, Heylmann, Alexandra S., Wessler, Patrícia G., Steckert, Amanda, Mastella, Gustavo A., de Oliveira, Mariana B., Damázio, Louyse S., Pacheco, Felipe D., Calixto, Octacílio P., Pereira, Flávio P., Macan, Tamires P., Pedro, Thayara H., Schuck, Patrícia F., Quevedo, João, and Budni, Josiane
- Subjects
- *
FOLIC acid , *OXIDATIVE stress , *SCHIZOPHRENIA , *DIETARY supplements , *KETAMINE , *ANIMAL disease models - Abstract
Recent studies have shown benefits for the supplementation of folic acid in schizophrenic patients. The aim of this study was to evaluate the effects of folic acid addition on adult rats, over a period of 7 or 14 days. It also sets out to verify any potential protective action using an animal model of schizophrenia induced by ketamine, in behavioral and biochemical parameters. This study used two protocols (acute and chronic) for the administration of ketamine at a dose of 25 mg/kg (i.p.). The folic acid was given by oral route in doses of 5, 10 and 50 mg/kg, once daily, for 7 and/or 14 days in order to compare the protective effects of folic acid. Thirty minutes after the last administration of ketamine, the locomotor and social interaction activities were evaluated, and immediately the brain structure were removed for biochemical analysis. In this study, ketamine was administered in a single dose or in doses over the course of 7 days increasing the animal’s locomotion. This study showed that the administration of folic acid over 7 days was unable to prevent hyper locomotion. In contrast, folic acid (10 and 50 mg/kg) administrated over a period of 14 days, was able to partially prevent the hyper locomotion. Our data indicates that both acute and chronic administrations of ketamine increased the time to first contact between the animals, while the increased latency for social contact was completely prevented by folic acid (5, 10 and 50 mg/kg). Chronic and acute administrations of ketamine also increased lipid peroxidation and protein carbonylation in brain. Folic acid (10 and 50 mg/kg) supplements showed protective effects on the oxidative damage found in the different brain structures evaluated. All together, the results indicate that nutritional supplementation with folic acid provides promising results in an animal model of schizophrenia induced by ketamine. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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45. The role of mTOR in depression and antidepressant responses.
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Abelaira, Helena M., Réus, Gislaine Z., Neotti, Morgana V., and Quevedo, João
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- *
MENTAL depression , *THERAPEUTICS , *ANTIDEPRESSANTS , *PHARMACODYNAMICS , *CELLULAR signal transduction , *TEXTBOOKS , *RAPAMYCIN , *PREFRONTAL cortex , *METHYL aspartate receptors , *PHYSIOLOGY - Abstract
Abstract: The aim of this study was to characterize the mTOR signaling cascade in depression and the actions that antidepressant drugs have on this pathway. Herein, a literature review was performed by verification and comparison of textbooks and journal articles that describe the characterization of the mTOR signaling cascade and its relationship to depression and antidepressant drugs, especially ketamine. Postmortem studies have shown robust deficits in the mammalian target of rapamycin (mTOR) signaling in the prefrontal cortex of subjects diagnosed with major depressive disorder. However, besides the mTOR signaling pathway having an antidepressant response to various drugs, this seems to be more associated with antidepressant N-methyl-d-aspartate (NMDA) receptor antagonists, such as ketamine. The characterization of the mTOR signaling pathway in depression and its action in response to antidepressants show great potential for the identification of new therapeutic targets for the development of antidepressant drugs. [Copyright &y& Elsevier]
- Published
- 2014
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46. Effects of pregabalin on behavioral alterations induced by ketamine in rats.
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Arcoverde Nunes, Emerson, Canever, Leila, de Oliveira, Larissa, D'altoe de Luca, Renata, Quevedo, João, Zugno, Alexandra, Peregrino, Antonio, Crippa, José Alexandre S., Dursun, Serdar M., Baker, Glen B., and Hallak, Jaime Eduardo C.
- Subjects
- *
PREGABALIN , *KETAMINE , *SCHIZOPHRENIA , *PSYCHOSES , *PLACEBOS - Abstract
Objective: The aim of this study is to investigate the effects of pregabalin on the behavior of rats under the influence of ketamine, an NMDA receptor antagonist that mimics the symptoms of schizophrenia. Methods: Rats were injected with saline or 25 mg/kg ketamine intraperitoneally. After that, behavior modifications were investigated by the evaluation of stereotypy and hyperlocomotion, after treating rats with pregabalin (at doses of 30 mg/kg or 100 mg/kg) or placebo (saline solution). Results: The administration of pregabalin reduced ketamine-induced hyperlocomotion. However, neither doses of pregabalin had a significant effect on ketamine- induced stereotypy. Conclusion: This is the first study to investigate the effects of pregabalin using an animal model of psychosis. Furthermore, our results indicate that behavioral changes induced by ketamine in rats can be reversed with'the use of pregabalin, suggesting its potential to treat psychotic symptoms. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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47. The role of NMDA receptor in neurobiology and treatment of major depressive disorder: Evidence from translational research.
- Author
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Amidfar, Meysam, Woelfer, Marie, Réus, Gislaine Z., Quevedo, João, Walter, Martin, and Kim, Yong-Ku
- Subjects
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METHYL aspartate receptors , *MENTAL depression , *NEUROBIOLOGY , *THERAPEUTICS , *TRANSLATIONAL research , *NEUROPLASTICITY - Abstract
There is accumulating evidence demonstrating that dysfunction of glutamatergic neurotransmission, particularly via N-methyl-d-aspartate (NMDA) receptors, is involved in the pathophysiology of major depressive disorder (MDD). Several studies have revealed an altered expression of NMDA receptor subtypes and impaired NMDA receptor-mediated intracellular signaling pathways in brain circuits of patients with MDD. Clinical studies have demonstrated that NMDA receptor antagonists, particularly ketamine, have rapid antidepressant effects in treatment-resistant depression, however, neurobiological mechanisms are not completely understood. Growing body of evidence suggest that signal transduction pathways involved in synaptic plasticity play critical role in molecular mechanisms underlying rapidly acting antidepressant properties of ketamine and other NMDAR antagonists in MDD. Discovering the molecular mechanisms underlying the unique antidepressant actions of ketamine will facilitate the development of novel fast acting antidepressants which lack undesirable effects of ketamine. This review provides a critical examination of the NMDA receptor involvement in the neurobiology of MDD including analyses of alterations in NMDA receptor subtypes and their interactive signaling cascades revealed by postmortem studies. Furthermore, to elucidate mechanisms underlying rapid-acting antidepressant properties of NMDA receptor antagonists we discussed their effects on the neuroplasticity, mostly based on signaling systems involved in synaptic plasticity of mood-related neurocircuitries. • Synaptic plasticity is critical for NMDA receptor antagonists effects. • Depressive patients show altered levels of NMDA receptor subunits in the brain. • Ketamine effects could be result of brain networks connectivity changes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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