1. Expression of S100A8/A9 in HaCaT keratinocytes alters the rate of cell proliferation and differentiation.
- Author
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Voss A, Bode G, Sopalla C, Benedyk M, Varga G, Böhm M, Nacken W, and Kerkhoff C
- Subjects
- Adaptation, Physiological, Cell Line, Epithelial Cells cytology, Filaggrin Proteins, Humans, Keratinocytes metabolism, NF-kappa B metabolism, Calgranulin A genetics, Calgranulin B genetics, Cell Differentiation, Cell Proliferation, Keratinocytes cytology
- Abstract
S100A8/A9 promotes NADPH oxidase in HaCaT keratinocytes and subsequently increases NFκB activation, which plays important roles in the balance between epidermal growth and differentiation. S100A8/A9-positive HaCaT cells present with a significantly reduced rate of cell division and greater expression of two keratinocyte differentiation markers, involucrin and filaggrin, than control cells. S100A8/A9 mutants fail to enhance NFκB activation, TNFα-induced IL-8 gene expression and NFκB p65 phosphorylation, and S100A8/A9-positive cells demonstrate better cell survival in forced suspension culture than mutant cells. S100A8/A9 is induced in epithelial cells in response to stress. Therefore, S100A8/A9-mediated growth arrest could have implications for tissue remodeling and repair., (Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2011
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