1. Sevoflurane induces cardioprotection through reactive oxygen species-mediated upregulation of autophagy in isolated guinea pig hearts.
- Author
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Shiomi M, Miyamae M, Takemura G, Kaneda K, Inamura Y, Onishi A, Koshinuma S, Momota Y, Minami T, and Figueredo VM
- Subjects
- Animals, Guinea Pigs, Hemodynamics drug effects, In Vitro Techniques, Male, Myocardial Infarction pathology, Myocardial Infarction prevention & control, Sevoflurane, Up-Regulation drug effects, Anesthetics, Inhalation pharmacology, Autophagy drug effects, Cardiotonic Agents pharmacology, Heart drug effects, Ischemic Preconditioning, Myocardial, Methyl Ethers pharmacology, Reactive Oxygen Species metabolism
- Abstract
Purpose: Sevoflurane increases reactive oxygen species (ROS), which mediate cardioprotection against myocardial ischemia-reperfusion injury. Emerging evidence suggests that autophagy is involved in cardioprotection. We examined whether reactive oxygen species mediate sevoflurane preconditioning through autophagy., Methods: Isolated guinea pigs hearts were subjected to 30 min ischemia followed by 120 min reperfusion (control). Anesthetic preconditioning was elicited with 2 % sevoflurane for 10 min before ischemia (SEVO). The ROS-scavenger, N-(2-mercaptopropionyl) glycine (MPG, 1 mmol/l), was administered starting 30 min before ischemia to sevoflurane-treated (SEVO + MPG) or non-sevoflurane-treated (MPG) hearts. Infarct size was determined by triphenyltetrazolium chloride stain. Tissue samples were obtained after reperfusion to determine autophagy-related protein (microtubule-associated protein light chain I and II: LC3-I, -II) and 5' AMP-activated protein kinase (AMPK) expression using Western blot analysis. Electron microscopy was used to detect autophagosomes., Results: Infarct size was significantly reduced and there were more abundant autophagosomes in SEVO compared with control. Western blot analysis revealed that the ratio of LC3-II/I and phosphorylation of AMPK were significantly increased in SEVO. These effects were abolished by MPG., Conclusions: Sevoflurane induces cardioprotection through ROS-mediated upregulation of autophagy.
- Published
- 2014
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