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Your search keyword '"Nikzad, Safoora"' showing total 3 results
Start Over Author "Nikzad, Safoora" Topic ionizing radiation
3 results on '"Nikzad, Safoora"'

2. The Cell Survival of F10B16 Melanoma and 4T1 Breast Adenocarcinoma Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods

Author

Nikzad, Safoora, Hashemi, Bijan, Hassan, Zuhair-Mohammad, and Mozdarani, Hossein

Subjects
MTT assay, 4T1 breast adenocarcinoma, Original Article, F10B16 melanoma, ionizing radiation, cell survival fraction, growing curve
Abstract

Background: various MTT assay methods are proposed to obtain the cell survival parameters. Objective: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation. Methods: a common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adenocarcinoma survivals after exposing them to ionizing radiation from a Co-60 machine. To obtain the survival parameters of the cells based on the common method, the cells were inoculated in 96-well plates. After irradiating the plates, the MTT assay was performed over the following days for a period of 8 days. Thereafter, the survival fraction was calculated from a simple equation for every day from which the best day was selected. To acquire the cells’ survival parameters based on the novel method, extensive experiments were performed on a large number of samples. Then, the MTT assay was done in every day following various experimental treatments to acquire the exponential growth. Finally, the cells’ survivals were determined by measuring the space between relevant growing curves. Results: at low doses (

Published
2013

3. MiR-185 enhances radiosensitivity of colorectal cancer cells by targeting IGF1R and IGF2.

Author

Afshar, Saeid, Najafi, Rezvan, Sedighi Pashaki, Abdolazim, Sharifi, Mohammadreza, Nikzad, Safoora, Gholami, Mohammad Hadi, khoshghadam, Alireza, Amini, Razieh, Karimi, Jamshid, and Saidijam, Massoud

Subjects
*COLON cancer treatment, *CELL proliferation, *MICRORNA, *APOPTOSIS, *IONIZING radiation
Abstract

Objective Radioresistance is a significant obstacle for effective treatment of colorectal cancer (CRC). Recent studies have indicated that miR-185 inhibits proliferation, survival, and invasion of CRC; however, the role of this miRNA in radioresistance of CRC has not been identified yet. The aim of this study is to investigate the role of miR-185 in radiosensitivity of CRC. Methods After transfecting the cells with mimic miR-185, expressions of IGF1R and IGF2 were evaluated by real-time PCR and western blot. The radiation response of transfected cells was also examined by colony forming assay. Sub-G1 fraction analysis through flow cytometry and caspase 3 activity was used to evaluate apoptosis. Results The results of real-time PCR and western blot indicated that IGF1R and IGF2 are downregulated in the transfected cells. Colony forming assay revealed that transfected cells were more radiosensitive than other cells. On the other hand,following irradiation the rate of apoptosis was significantly higher in the transfected cells than in the other cells. Conclusion In summary, our study is the first to show that upregulation of miR-185 enhances the sensitivity of CRC cells to ionizing radiation. miR-185 may act as a novel biomarker of radioresistance and may clinically enhance the radiation response of CRC. [ABSTRACT FROM AUTHOR]

Published
2018
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