1. Sulfated β-(1→4)-galacto-oligosaccharides and their effect on angiogenesis
- Author
-
D. H. Paper, Christian W Käsbauer, and Gerhard Franz
- Subjects
Molecular Sequence Data ,Ion chromatography ,Neovascularization, Physiologic ,Oligosaccharides ,Angiogenesis Inhibitors ,Chick Embryo ,Polysaccharide ,Fibroblast growth factor ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Sulfation ,Allantois ,Polysaccharides ,Carbohydrate Conformation ,Animals ,Sulfate ,chemistry.chemical_classification ,Plants, Medicinal ,Chromatography ,Organic Chemistry ,Fabaceae ,Chorion ,General Medicine ,Oligosaccharide ,Angiogenesis inhibitor ,Carbohydrate Sequence ,chemistry ,Carbohydrate conformation - Abstract
Sulfated beta-(1-->4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO3-pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content was determined by ion chromatography. DP 5 and higher showed a pronounced antiangiogenic effect with scores of 0.9-1.2 for DP 7-9 using the CAM-assay. An interaction with the fibroblast growth factor FGF-2 was noticed for DP 4-12 depending on the degree of sulfation using the FGF-2-trypsin assay.
- Published
- 2001