1. The relation of omentin gene expression and glucose homeostasis of visceral and subcutaneous adipose tissues in non-diabetic adults.
- Author
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Daneshafrooz A, Yuzbashian E, Zarkesh M, Asghari G, Mirmiran P, Hedayati M, Abooshahab R, Fanaei SM, and Khalaj A
- Subjects
- Adult, Aged, Aged, 80 and over, Case-Control Studies, Cross-Sectional Studies, Cytokines metabolism, Fasting blood, Female, GPI-Linked Proteins genetics, GPI-Linked Proteins metabolism, Homeostasis, Humans, Insulin blood, Lectins metabolism, Male, Middle Aged, Triglycerides blood, Young Adult, Blood Glucose analysis, Cytokines genetics, Insulin Resistance genetics, Intra-Abdominal Fat metabolism, Lectins genetics, Subcutaneous Fat metabolism, Up-Regulation
- Abstract
Background: Adipose tissue (AT) is a passive reservoir for energy storage and an active endocrine organ responsible for synthesizing bioactive molecules called adipokines. Omentin is known as an anti-inflammatory adipokine that can modulate insulin sensitivity. The present study aimed to investigate the relationship between omentin mRNA expression and glucose homeostasis of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) in non-diabetic adults., Methods: VAT and SAT adipose tissues were collected from 137 adults aged ≥ 18 years hospitalized for abdominal surgery. Before surgery, preoperative blood samples were taken from the participants to measure fasting plasma glucose, insulin, and triglyceride. BMI, HOMA-IR, HOMA-B, and QUICKI were calculated. Insulin levels were measured with Mercodia kits using enzyme-linked immunosorbent assay (ELISA). In order to obtain omentin mRNA expression, real-time PCR was performed., Results: Overall, 91 (66.4%) subjects were healthy [without insulin resistance (IR)], and 46 (33.6%) participants were with IR. In healthy and IR subjects, omentin gene expression was 1.04 and 2.32, respectively in VAT, and 3.06 and 1.30, respectively, in SAT (P > 0.05). After controlling for age and BMI, linear regression analysis indicated a significant positive association of SAT omentin expression with insulin concentration (β = 0.048; 95% CI 0.009, 0.088, P = 0.017) and HOMA-IR (β = 0.173; 95% CI 0.023, 0.323, P = 0.014). Moreover, a negative association of SAT omentin expression with HOMA-B (β = - 0.001; 95% CI 0.002, - 0.001, P < 0.001) was observed., Conclusion: This study's finding confirms a direct association between IR with omentin mRNA levels in SAT. Besides, the indicator of insulin sensitivity had an inverse association with omentin gene expression in SAT. This aspect of research suggests that omentin secretion from SAT has a strong link with insulin regulation., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
- Published
- 2022
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