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Objective: To investigate whether intraluminal administration of oxygenated perfluorocarbons (PFCs) protects the enterocyte from acute ischemia-reperfusion (I/R) injury., Materials and Methods: Twenty rabbits were divided in 4 groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of oxygenated PFCs 30 minutes before ischemia (group C), and acute I/R plus infusion of oxygenated PFCs 30 minutes before reperfusion (group D). Serum creatine phosphokinase (CPK) and mucosal disaccharidase activity were examined. Intestinal biopsies were obtained for electron microscopy study., Results: Group B CPK mean values are 3495.2 ± 157.35 and 4855 ± 350.21 U/L. Group C: 2674.6 ± 265.87 and 3231 ± 232.30. Group D: 2382.2 ± 102.90 and 3217.6 ± 185.61 at 120 and 180 minutes (P<.05). At 180 minutes, maltase and sucrose values were 33.63, 51.88, 8.45, and 19.91, and 17.99, 22.87, 6.62, and 14.24 µmol/min per g for groups A, B, C, and D, respectively (P<.05). Histopathology showed the least cellular deterioration in PFC groups., Conclusion: Oxygenated PFCs protect the enterocyte during bowel I/R., (© The Author(s) 2011)

Objective: To evaluate the effect of intraluminal administration of oxygenated perfluorocarbons (PFCs) on small intestine's viability in an experimental model of acute ischemia-reperfusion (I/R)., Methods: Twenty rabbits were divided in four groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of PFCs 30 min before ischemia (group C), and acute I/R plus infusion of PFCs 30 min before reperfusion (group D). Malondialdehyde (MDA) tissue levels and d-lactate blood samples were taken. All tissue sections were examined under light microscope., Results: Mean MDA levels in group A: 1.79 +/- 0.97 at 0 min, 2.25 +/- 1.76 at 120 min and 3.70 +/- 1.76 nmols/g at 180 min. Group B: 2.60 +/- 0.58 at 0 min, 4.20 +/- 0.58 at 120 min and 5.48 +/- 2.01 at 180 min. Group C: 1.54 +/- 0.85 at 0 min, 1.14 +/- 0.37 at 120 min and 0.59 +/- 0.35 at 180 min. Group D: 2.12 +/- 0.62 at 0 min, 3.97 +/- 0.70 at 120 min and 2.32 +/- 0.37 at 180 min (p < 0.05). Mean d-lactate levels in group A: at 0 min 36.45 +/- 1.99, at 120 min 39.10 +/- 2.37 and at 180 min 40.05 +/- 2.13 mg/dl. Group B: 61.23 +/- 11.03 at 0 min, 74.84 +/- 10.70 at 120 min and 89.90 +/- 9.29 at 180 min. Group C: at 0 min 51.05 +/- 10.36, at 120 min 56.07 +/- 11.27 and at 180 min 57.20 +/- 11.19. Group D: 64.36 +/- 5.26 at 0 min, 72.55 +/- 7.19 at 120 min and 77.02 +/- 9.41 at 180 min (p < 0.05). Histopathological analysis indicated a significant improvement in the groups of oxygenated PFCs compared with I/R group., Conclusion: Intraluminal administration of oxygenated PFCs seems that protect the intestine from the I/R injury.