1. Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon
- Author
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Mathieu Nabot, Magali Chabé, Monzer Hamze, Eric Viscogliosi, Gaël Even, Manasi Sawant, Sadia Benamrouz-Vanneste, Salma Khaled, Aisha Ayoubi, Gabriela Certad, Dima El Safadi, Nausicaa Gantois, Jinane El Houmayraa, Fouad Dabboussi, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Université Libanaise, Gènes Diffusion [Douai], Plateforme d'expertises génomiques appliquées aux sciences expérimentales [Lille] (PEGASE-Biosciences), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Concern Worldwide, Solidarités International, Faculté de gestion, économie et sciences [UCL, Lille] (FGES), Université catholique de Lille (UCL), Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), This study was supported by the Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale, the Institut Pasteur of Lille, the University of Lille, the University Catholic of Lille, the CHRU of Lille, the Lebanese University and the non-governmental organizations Concern Worldwide and Solidarités International. SK and MS were supported by PhD fellowships from the AZM & Saade Association of Lebanon and University of Lille, respectively., Université Catholique de Lille - Faculté de gestion, économie et sciences (FGES), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), and Viscogliosi, Eric
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,media_common.quotation_subject ,030231 tropical medicine ,Population ,Intestinal parasite ,Biology ,intestinal parasites ,medicine.disease_cause ,Microbiology ,molecular epidemiology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Hygiene ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Virology ,Epidemiology ,medicine ,risk factors ,North Lebanon ,[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Risk factor ,education ,lcsh:QH301-705.5 ,Blastocystis sp ,media_common ,education.field_of_study ,Molecular epidemiology ,Transmission (medicine) ,internal tented settlements ,transmission ,3. Good health ,Diarrhea ,SSU rDNA subtyping ,030104 developmental biology ,lcsh:Biology (General) ,[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Syrian refugees ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,medicine.symptom ,real-time PCR ,[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology ,Demography - Abstract
International audience; Molecular data concerning the prevalence and subtype (ST) distribution of the intestinal parasite Blastocystis sp. remain scarce in the Middle East. Accordingly, we performed the first molecular epidemiological survey ever conducted in the Syrian population. A total of 306 stool samples were collected from Syrian refugees living in 26 informal tented settlements (ITS) subjected or not to water, sanitation, and hygiene (WASH) interventions in North Lebanon, then screened for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping. The overall prevalence of the parasite was shown to reach 63.7%. Blastocystis sp. colonization was not significantly associated with gender, age, symptomatic status, abdominal pain or diarrhea. In contrast, WASH intervention status of ITS was identified as a risk factor for infection. Among a total of 164 subtyped isolates, ST3 was predominant, followed by ST1, ST2, and ST10. No particular ST was reported to be associated with age, gender, symptomatic status, digestive disorders, or WASH intervention status of ITS. Intra-ST diversity of ST1 to ST3 was low suggesting large-scale anthroponotic transmission. Moreover, comparative analysis of ST1 to ST3 genotypes revealed that the circulation of the parasite between Syrian refugees and the host population was likely limited.
- Published
- 2021
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