1. In vivo macrophage recruitment by murine intervertebral disc cells.
- Author
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Rand NS, Dawson JM, Juliao SF, Spengler DM, and Floman Y
- Subjects
- Animals, Cell Movement, Intervertebral Disc pathology, Intervertebral Disc Displacement pathology, Male, Mice, Mice, Inbred C57BL, Discitis etiology, Intervertebral Disc physiopathology, Intervertebral Disc Displacement complications, Intervertebral Disc Displacement physiopathology, Macrophages physiology
- Abstract
Summary: An in vivo murine experiment was conducted to measure the capacities of viable intervertebral disc cells to recruit inflammatory cells. The objective was to determine whether compounds secreted from viable cells induce inflammation or whether inflammation in disc herniation simply requires exposure to structural cell or matrix components. Three tissue preparations were inserted into the right lower peritoneal cavity of male mice: tissue with viable annulus fibrosus and nucleus pulposus cells, tissue with viable annulus fibrosus cells, or devitalized annulus fibrosus and nucleus pulposus tissue. Controls included sham-operated and nonoperated groups. Mice were killed 1, 2, or 7 days after surgery. Macrophage recruitment occurred after exposure to viable disc tissue but not after exposure to devitalized disc components; recruitment increased over time. Viable disc cells play a role in the etiology of inflammation in disc herniation.
- Published
- 2001
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