47,746 results on '"Urine"'
Search Results
2. THE ROLE OF GAMMA-GLUTAMYL TRANSPEPTIDASE IN THE INTERNAL DISEASES CLINIC.
- Author
-
ORLOWSKI M
- Subjects
- Bile, Biliary Tract, Blood Chemical Analysis, Cardiovascular Diseases, Cholangitis, Cholecystitis, Cholelithiasis, Clinical Enzyme Tests, Hepatitis, Hepatitis A, Internal Medicine, Jaundice, Jaundice, Obstructive, Kidney Diseases, Liver Cirrhosis, Liver Diseases, Neoplasms, Pancreatitis, Transferases, Urine, gamma-Glutamyltransferase
- Published
- 1963
3. [SYMPOSIUM ON NEW DIAGNOSTIC PROCEDURES IN INTERNAL MEDICINE. 2. THE APPLICATION OF THE AUTOANALYZER TO THE ROUTINE LABORATORY].
- Author
-
MOTEGI K
- Subjects
- Blood Chemical Analysis, Chlorides, Cholesterol blood, Creatine, Creatinine, Equipment and Supplies, Internal Medicine, Nitrogen, Potassium, Sodium, Urea, Urine
- Published
- 1963
4. Kidney Cancer and Potential Use of Urinary Extracellular Vesicles
- Author
-
Linh Nguy-Hoang Le, Javaria Munir, Eun-Bit Kim, and Seongho Ryu
- Subjects
urine ,extracellular vesicle ,exosome ,kidney cancer ,renal cell carcinoma ,biomarker ,Other systems of medicine ,RZ201-999 ,Internal medicine ,RC31-1245 - Abstract
Kidney cancer is the 14th most common cancer globally. The 5-year relative survival rate of kidney cancer at a localized stage is 92.9% and it declines to 17.4% in metastatic stage. Currently, the most accurate method of its diagnosis is tissue biopsy. However, the invasive and costly nature of biopsies makes it undesirable in many patients. Therefore, novel biomarkers for diagnosis and prognosis should be explored. Urinary extracellular vesicles (uEVs) are small vesicles (50–200 nm) in urine carrying nucleic acids, proteins and lipids as their cargos. These uEVs’ cargos can provide non-invasive alternative to monitor kidney health. In this review, we have summarized recent studies investigating potential use of uEVs’ cargos as biomarkers in kidney cancer for diagnosis, prognosis and therapeutic intervention.
- Published
- 2024
- Full Text
- View/download PDF
5. Epidemiological profile of funguria in an University Hospital in Oujda, Morocco
- Author
-
Adil Maleb, Aziza Hami, Somiya Lambrabet, Safaa Rifai, Nawal Rahmani, Mohammed Bensalah, Elmostafa Benaissa, Yassine Ben Lahlou, Mohammed Frikh, and Mostafa El Ouennass
- Subjects
funguria ,infection ,urinary tract infection ,urine ,yeast ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Background and Purpose: The presence of yeasts in the urine is not synonymous with urinary tract infection since it can result in simple colonization or contamination. Regarding this, it is required to further clarify the epidemiological profile of funguria. Accordingly, the present study was conducted to establish the epidemiology of funguria in the Mohammed VI Teaching Hospital of Oujda, Morocco. Materials and Methods: This retrospective study was conducted on all urine samples sent for cytobacteriological examination to a microbiology laboratory over a period of 28 months (i.e., from March 2016 to June 2018). After the removal of duplicates, the urine samples were treated according to the recommendations of the medical microbiology standards. Results: A total of 15,165 urine samples were collected. Urinary colonization accounted for 4.94% (n=749) of cases. The infections of the urinary tract accounted for 5.35% (n=811) of cases. Microbial isolates (n=1,669) in colonization and urinary tract infections were dominated by bacteria (93.47%, n=1,560). Furthermore, the yeasts accounted for 6.53% (n=109) of the isolates. Candida albicans was isolated from 56.88% (n=62) of funguria cases. The risk factors for funguria in our series were essentially old age, admission to intensive care unit, and broad-spectrum antibiotic therapy. Conclusion: The current level of knowledge about the clinical situations leading to funguria with the improvement and popularization of efficient identification techniques for yeasts other than C. albicans should redress the epidemiology of funguria. This should allow the knowledgeable societies to establish the rules of interpreting the cytobacteriological examination of the urine in case of funguria, as for bacteriuria.
- Published
- 2020
- Full Text
- View/download PDF
6. Mucormycosis: A comparative update between conventional and molecular diagnosis strategies
- Author
-
Shikha Moudgal, Shalinee Rao, and Manju Pai
- Subjects
angio-invasive ,aspergillus ,molecular methods ,mucor ,mucormycosis ,pcr ,rhizopus ,serum ,urine ,Internal medicine ,RC31-1245 ,Biology (General) ,QH301-705.5 - Abstract
Mucormycosis is an opportunistic, aggressive, and angioinvasive fungal infection associated with a high mortality rate as it disseminates and infects the whole body if not treated early. Most conventional diagnostic methods require time and may also generate false-negative reports due to the several lacunae associated. On the other hand, molecular methods are rapid, reliable, and can be applied to different biological samples, such as fresh tissue, formalin-fixed paraffin-embedded blocks, serum, and urine. Mucorales are angio-invasive, and many studies have found the circulating fungal DNA (a non-invasive form of DNA) in the blood and urine of the patient. In addition, with the increase in the usage of steroid drugs in this COVID scenario, the rate of mucormycosis infection has taken a sudden rise. In light of this situation, there is an imperative need to diagnose these infections at the earliest.Studies on the diagnosis of mucormycosis were explored and retrieved from Pubmed and Google Scholar. Histopathology and culture reports of patients diagnosed with mucormycosis in 2019-2020 were searched and retrieved from the hospital database (ethically permitted) to find the rate of concordance between the techniques.In this review study, different tested molecular techniques are encapsulated, and various primers are documented that are used to identify and classify different Mucoromycota fungi.An attempt has been made to compare the feasibility, accuracy adaptability, and time frame used among molecular and conventional methods for the diagnosis of mucormycosis.
- Published
- 2022
- Full Text
- View/download PDF
7. Urine biomarkers for monitoring acute kidney injury in premature infants
- Author
-
Yo Han Ahn, Juyoung Lee, Jiyoung Chun, Yong Hoon Jun, and Tae-Jung Sung
- Subjects
acute kidney injury ,biomarker ,gestational age ,premature infants ,urine ,Internal medicine ,RC31-1245 ,Specialties of internal medicine ,RC581-951 - Abstract
Background : : Premature infants are at high risk for acute kidney injury (AKI). Serum creatinine (Cr) has limitations for evaluating kidney function in premature infants. We evaluated whether urine biomarkers could be used to monitor AKI in premature infants. Methods : : A prospective cohort study was conducted among infants born at < 37 weeks. Urine biomarkers and serum Cr were measured on postnatal days 1, 3, 5, 7, 10, and 14. Infants were divided into 3 groups according to gestational age (GA); < 28, 28 to < 32 and 32 to < 37 weeks. Results : : AKI occurred in 17 of 83 (20.5%) recruited infants at a median age of 7 (interquartile range 5–10) days. While the most common cause of AKI was hemodynamically significant patent ductus arteriosus (53.8%) in infants of GA < 28 weeks, necrotizing enterocolitis was the leading cause (50.0%) in infants of GA 28 to < 32 weeks. Urinary levels of neutrophil-gelatinase-associated lipocalin/Cr were higher and epidermal growth factor/Cr were lower in AKI group before the onset of AKI in infants of GA < 28 weeks. In infants of GA 28 to < 32 weeks, urinary interleukin-8/Cr levels were higher in AKI group at approximately the time of AKI onset. Conclusion : : Several urine biomarkers were significantly different between AKI and no AKI groups, and some had changed before the onset of AKI. These groups were distinct according to causative factors of AKI and GA. Urine biomarkers could be useful for monitoring the development of AKI in premature infants.
- Published
- 2020
- Full Text
- View/download PDF
8. Clinical and diagnostic value of impairments in electrolyte metabolism in children with acquired myopia
- Author
-
T.Ye. Tsybulska, S.V. Gorbachova, and T.S. Zavgorodnia
- Subjects
myopia ,connective tissue dysplasia ,diagnostics ,electrolytes ,urine ,children ,Internal medicine ,RC31-1245 - Abstract
Background: Identifying the biochemical features of the body in myopia associated with connective tissue dysplasia is still important in the ophthalmologist’s practice. Purpose: To assess the clinical and diagnostic value of impairments in electrolyte merabolism in children with acquired myopia. Methods: Group 1 included 30 children with mild myopia associated with the syndrome of unspecified connective tissue dysplasia (SUCTD), and Group 2 included 30 children with mild myopia without SUCTD. Group 3 (controls) included 30 pediatric healthy controls without ocular disease. Electrolyte balance was assessed by measuring magnesium, calcium, sodium, potassium and chloride ion concentrations in daily urine. Results: Magnesium and calcium ion concentrations in daily urine in children in Group 1 were significantly (2- and 1.7-fold, respectively) lower compared with children in Group 2, and significantly (2.1- and 1.9-fold, respectively) lower compared with controls (р < 0.05). There was no difference in sodium, potassium or chloride ion concentrations in daily urine among study groups. ROC analysis found that an optimum cutoff magnesium ion concentration of ≤2.3 mmol/day in daily urine was 97% sensitive and 80% specific, with an area under curve value (AUC) of 0.97±0.12 (CI 0.95-0.99) (р < 0.0001). In addition, an optimum cutoff calcium ion concentration of ≤3.42 mmol/day in daily urine was 96% sensitive and 85% specific, with an AUC value of 0.94±0.27 (CI 0.88-0.98) (р < 0.0001). Magnesium ion concentration in daily urine was moderately negatively correlated with dysplasia severity (r= -0.65, р < 0.05), and calcium ion concentration was moderately negatively correlated with dysplasia severity (r= -0.59, р < 0.05). Conclusion: The current study determined the diagnostic values of magnesium and calcium ion concentrations (≤ 2.3 mmol/day and ≤ 3.42 mmol/day, respectively) which can be used as biomarkers for laboratory screening for the presence of SUCTD in clinical practice.
- Published
- 2019
- Full Text
- View/download PDF
9. LncRNA-miRNA-mRNA expression variation profile in the urine of calcium oxalate stone patients
- Author
-
Xiongfa Liang, Yongchang Lai, Weizhou Wu, Dong Chen, Fangling Zhong, Jian Huang, Tao Zeng, Xiaolu Duan, Yapeng Huang, Shike Zhang, Shujue Li, and Wenqi Wu
- Subjects
Calcium oxalate ,Kidney stone ,Competing endogenous RNA network ,Urine ,LncRNA ,MiRNA ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background To explore long-non-coding RNA (lncRNA), microRNA (miRNA) and messenger RNA (mRNA) expression profiles and their biological functions in the urine samples in calcium oxalate (CaOx) patients. Methods Five CaOx kidney stone patients were recruited in CaOx stone group and six healthy people were included as control group, whose midstream morning urine was collected before the patients were given any medicine on admission. After total RNA was extracted from urine, microarray of miRNA, mRNA and lncRNA were applied to explore their expression variation. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to reveal the gene functions of the dysregulated lncRNA-associated competing endogenous RNA (ceRNA) network. Quantitative real-time PCR were performed on HK-2 cells treated with sodium oxalate (NaOx) to further screen out the differentially expression profiles of these RNAs. Results A total of nine miRNAs, 883 mRNAs and 1002 lncRNAs were differentially expressed in urine of CaOx patients compared with normal population. GO analysis revealed that most of mRNAs from ceRNA network were enriched in terms of respiratory burst, regulation of mitophagy, and protein kinase regulator activity. KEGG pathway analysis of these genes related to ceRNA network highlight their critical role in pentose phosphate pathway, glyoxylate and dicarboxylate metabolism, and Janus kinase/signal transducer and activator of transcription (JAK-STAT) signaling pathway. Five miRNAs (miR-6796-3p, miR-30d-5p, miR-3192–3p, miR-518b and miR-6776-3p), four mRNAs (NT5E, CDH4, CLEC14A, CCNL1) and six lncRNAs (lnc-TIGD1L2–3, lnc-KIN-1, lnc-FAM72B-4, lnc-EVI5L-1, lnc-SERPINI1–2, lnc-MB-6) from the HK-2 cells induced by NaOx were consistent with the expression changes of microarray results. Conclusion The differential expressed miRNAs, mRNAs and lncRNAs may be associated with numerous variations of the signaling pathways or regulation of metabolism and kinase activity, providing potential biomarkers for early diagnosis of urolithiasis and new basis for further research of urolithiasis mechanism.
- Published
- 2019
- Full Text
- View/download PDF
10. New alternatives for the treatment of uncomplicated urinary tract infection, presentation of 2 clinical cases
- Author
-
Andrés F. Reyes and Roberto Ramírez Marmolejo
- Subjects
urine ,urinary bladder diseases ,focal infection ,complementary therapies ,Internal medicine ,RC31-1245 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
The current perspective on the management of infectious diseases is a challenge for clinicians, because while new antibiotics are developed, bacteria improves its resistance system. That is why this study presents CANTHARIS 9 CH as an alternative treatment for urinary tract infection. This drug has an homeopathic use and it has been tested on humans as an urinary prophylactic. In this text, we present two cases of patients who voluntarily accept to receive this new protocol. Both cases show laboratory confirmation of infection, with quinolone resistance, among others, and negativization post-treatment with CANTHARIS 9 CH. No side effects were reported in this sample.
- Published
- 2018
11. How many urinalysis and urine cultures are necessary?
- Author
-
Laan, Bart J., van Horrik, Tessa M.Z.X.K., Nanayakkara, Prabath W.B., and Geerlings, Suzanne E.
- Subjects
- *
URINALYSIS , *URINE , *INTERNAL medicine , *URINARY catheters , *MICROSCOPY - Abstract
• Unnecessary urine testing is very common, especially in the emergency department. • Unnecessary urine testing could lead to overtreatment and unnecessary costs. • Reflex urine testing cancellation could prevent more than halve of microscopic analysis. • Reflex urine testing cancellation could prevent almost a fourth of urine cultures. Urinalysis and urine culture are two of the most commonly ordered tests. A positive urine test in asymptomatic patients often leads to overtreatment. Antimicrobials for asymptomatic bacteriuria is one of the most common unnecessary treatments. We aimed to explore the current ordering patterns of urinalysis and cultures. This is a substudy of the multicentre RICAT-trial, a successful quality improvement project to reduce inappropriate use of intravenous and urinary catheters in seven hospitals in the Netherlands. Adult patients with a (central or peripheral) venous or urinary catheter admitted to internal medicine and non-surgical subspecialty wards were eligible for inclusion. Data were collected every other week during baseline (seven months) and intervention periods (seven months). The primary outcome was the proportion of urine cultures performed following a negative urinalysis, i.e. dipstick and/or microscopic analysis, within 24 h. Between September 2016 and April 2018, we included 3748 patients, of which 3111 (83%) were admitted from the emergency department. Urinalysis and/or urine cultures were obtained in 2610 (70%) of 3748 patients. 626 (23.7%) of 2636 urine cultures and 1351 (55.8%) of 2419 microscopic analysis were unnecessary performed after a negative urinalysis. Cancelling urine testing orders after a negative dipstick would have saved almost € 19.500 during the study period in these seven hospitals. Unnecessary urine testing is frequent in non-surgical patients in the Netherlands. We need to take action to reduce unnecessary urinalysis and cultures, and thereby probably reduce overtreatment of asymptomatic bacteriuria. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
12. Sustained reduction in catheter-associated urinary tract infections using multi-faceted strategies led by champions: A quality improvement initiative
- Author
-
Nicholas A Turner, Sarah S. Lewis, Rebekah Wrenn, Chris D Sova, Becky Smith, Staci S. Reynolds, and Sonali D Advani
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Cross Infection ,Quality management ,Catheters ,Epidemiology ,business.industry ,Urinary system ,Urine ,Quality Improvement ,Article ,Catheter ,Intensive Care Units ,Infectious Diseases ,Intensive care ,Internal medicine ,Catheter-Related Infections ,Urinary Tract Infections ,Medicine ,Humans ,business ,Urinary Catheterization - Abstract
We reviewed the sustainability of a multifaceted intervention on catheter-associated urinary tract infection (CAUTI) in 3 intensive care units. During the 4-year postintervention period, we observed reductions in urine culture rates (from 80.9 to 47.5 per 1,000 patient days; P < .01), catheter utilization (from 0.68 to 0.58; P < .01), and CAUTI incidence rates (from 1.7 to 0.8 per 1,000 patient days; P = .16).
- Published
- 2023
13. The Accumulation of Gut Microbiome–derived Indoxyl Sulfate and P-Cresyl Sulfate in Patients With End-stage Renal Disease
- Author
-
Wangqun Liang, Xuechun Lin, Piwei Zhang, Ying Yao, Xiaolei Guo, Li Li, Siyun Xiang, Shuiqing He, Hong Wang, Xuezhi Zuo, Qianqian Xiong, Chenjiang Ying, and Jing Zhao
- Subjects
medicine.medical_specialty ,Indoles ,Medicine (miscellaneous) ,Urine ,Sulfuric Acid Esters ,medicine.disease_cause ,End stage renal disease ,Cresols ,chemistry.chemical_compound ,Downregulation and upregulation ,Dialysis Solutions ,RNA, Ribosomal, 16S ,Internal medicine ,Humans ,Medicine ,Renal Insufficiency, Chronic ,Sulfate ,Escherichia coli ,Feces ,Nutrition and Dietetics ,biology ,Sulfates ,business.industry ,Tryptophan ,biology.organism_classification ,Gastrointestinal Microbiome ,Endocrinology ,chemistry ,Nephrology ,Kidney Failure, Chronic ,Indoxyl Sulfate ,business ,Indican ,Bacteria - Abstract
Indoxyl sulfate (IS) and p-cresyl sulfate (pCS) are two important gut microbiota-generated protein-bound uremic toxins. The present study aims to explore the alterations of serum IS and pCS concentrations, their production, and daily removal in end-stage renal disease (ESRD).A case-controlled study was conducted based on 11 patients with ESRD and 11 healthy volunteers. The metabolic processes for IS and pCS were compared in these two groups, including gut microbiome, fecal indole and p-cresol, indole-producing bacteria and p-cresol-producing bacteria, serum total IS and pCS concentrations, and their daily removal by urine and spent dialyzate.Compared with healthy controls, patients with ESRD exhibited higher relative abundance of the indole-producing bacteria Escherichia coli (P .001) and Bacteroides fragilis (P = .010) and p-cresol-producing bacteria Bacteroides fragilis (P = .010) and Bacteroides caccae (P = .047). The predicted functional profiles of gut microbiome based on 16S rRNA gene PhyloChip analysis showed that the microbial tryptophan metabolism pathway (map00380, P = .0006) was significantly enriched in patients with ESRD. However, the fecal precursors indole (P = .332) and p-cresol concentrations (P = .699) were comparable between the two groups. The serum IS (P .001) and pCS (P .001) concentrations were far higher in patients with ESRD than those in healthy controls, whereas the daily total removal by urine and dialyzate was much lower for the former than that for the latter (P = .019 for IS, P = .016 for pCS).The present study showed serious IS and pCS accumulation in patients with ESRD, with significant expansion of indole-producing bacteria and p-cresol-producing bacteria, upregulation of the bacterial tryptophan metabolism pathway, and greatly increased serum IS and pCS concentrations, whereas significant decline of daily IS and pCS removal.
- Published
- 2022
14. The impact of a modified carbohydrate formula, and its constituents, on glycaemic control and inflammatory markers: A nested mechanistic sub-study
- Author
-
David Sturgess, Josephine M. Forbes, Jeff S. Coombes, Helen L. Barrett, Kye Gregory, Debbie Tolcher, Ra'eesa Doola, Alwyn S Todd, Casper G. Schalkwijk, Adam M. Deane, Satomi Okano, Interne Geneeskunde, and RS: Carim - V01 Vascular complications of diabetes and metabolic syndrome
- Subjects
Blood Glucose ,Glycation End Products, Advanced ,medicine.medical_specialty ,medicine.medical_treatment ,Critical Illness ,critically ill ,Receptor for Advanced Glycation End Products ,Medicine (miscellaneous) ,Inflammation ,Urine ,Glycemic Control ,THERAPY ,DIET ,Enteral Nutrition ,advanced glycation end-products ,Interquartile range ,Glycation ,HYPERGLYCEMIA ,Internal medicine ,medicine ,Dietary Carbohydrates ,Humans ,Food, Formulated ,Nutrition and Dietetics ,RECEPTOR ,business.industry ,Insulin ,Carbohydrate ,INSULIN ,Confidence interval ,Glucerna ,Endocrinology ,glycaemic control ,carbohydrate ,medicine.symptom ,business ,GLYCATION END-PRODUCTS ,CRITICALLY-ILL PATIENTS ,Biomarkers - Abstract
Background Hyperglycaemia occurs frequently in the critically ill. Dietary intake of advanced glycation end-products (AGEs), specifically N epsilon-(carboxymethyl)lysine (CML), may exacerbate hyperglycaemia through perturbation of insulin sensitivity. The present study aimed to determine whether the use of nutritional formulae, with varying AGE loads, affects the amount of insulin administered and inflammation. Methods Exclusively tube fed patients (n = 35) were randomised to receive Nutrison Protein Plus Multifibre (R), Diason (R) or Glucerna Select (R). Insulin administration was standardised according to protocol based on blood glucose (
- Published
- 2022
15. Drug Resistance, Rather than Low Tenofovir Levels in Blood or Urine, Is Associated with Tenofovir, Emtricitabine, and Efavirenz Failure in Resource-Limited Settings
- Author
-
Tracy Kellermann, Monica Gandhi, Eric H Decloedt, Gert U. van Zyl, Matthew A Spinelli, Zukiswa Nkantsu, Marije van Schalkwyk, Dolphina Cogill, Catherine Orrell, and Lauren Jennings
- Subjects
Cyclopropanes ,medicine.medical_specialty ,Efavirenz ,Anti-HIV Agents ,Immunology ,Drug Resistance ,HIV Infections ,Urine ,Drug resistance ,Emtricitabine ,Outcomes Research ,South Africa ,chemistry.chemical_compound ,Virology ,Internal medicine ,medicine ,Humans ,Viremia ,Tenofovir ,business.industry ,Viral Load ,Benzoxazines ,Regimen ,Cross-Sectional Studies ,Infectious Diseases ,chemistry ,Alkynes ,Dolutegravir ,business ,Viral load ,HIV drug resistance ,medicine.drug - Abstract
Introduction The high cost of viral load (VL) testing limits its use for antiretroviral treatment (ART) adherence support. A low-cost lateral flow urine tenofovir (TFV) rapid assay predicts PrEP breakthroughs but has not yet been investigated in HIV treatment. We therefore evaluated its utility in a pilot cross-sectional study of TFV-containing ART recipients at increased risk of virologic failure. Methods Participants who had a treatment interruption ≥30 days or had ≥1 episode of viremia (VL≥400 copies/mL) in the previous year were recruited from a public health setting in Cape Town, South Africa. Self-reported adherence data were collected, the urine TFV assay performed, and concurrent TFV-diphosphate (DP) analysed in dried blood spots. VL testing was done concurrently and, if viremic, genotypic HIV drug resistance testing performed. Results Of 48 participants, 18 (37.5%) had virologic failure (VL>400 copies/mL) at the time of the study including 16 of 39 receiving efavirenz (EFV), 2 of 6 receiving protease inhibitors (PI) and 0 of 3 receiving dolutegravir (DTG). Resistance testing succeeded in 17/18, of which 14 had significant mutations compromising ≥2 agents of the current EFV-based regimen. Of these 14, all had detected urine TFV. Urine TFV was undetectable in 2 out of 3 without regimen-relevant resistance; p=0.02. Conclusion In participants on EFV-based regimens returning to care, virologic failure was largely due to viral resistance, where detectable urine TFV had 100% sensitivity (14/14 participants) in predicting resistance. Conversely, when undetectable, the urine-based assay could be used to preclude participants with poor adherence from undergoing costly HIV drug resistance testing.
- Published
- 2022
16. Preoperative polymicrobial urine culture: An analysis of the risk of perioperative urinary tract infection
- Author
-
Bastien Gondran-Tellier, V. Guerin, Michael Baboudjian, V. Pauly, J. Albanese, Robin McManus, Eric Lechevallier, and C. Breuleux
- Subjects
Male ,medicine.medical_specialty ,medicine.drug_class ,Urology ,Urinary system ,Antibiotics ,Urine ,Postoperative Complications ,Internal medicine ,medicine ,Clinical endpoint ,Humans ,Antibiotic prophylaxis ,Pyuria ,Retrospective Studies ,business.industry ,Ceftriaxone ,Perioperative ,Anti-Bacterial Agents ,Urinary Tract Infections ,Female ,medicine.symptom ,business ,medicine.drug - Abstract
Summary Purpose To report our management of preoperative polymicrobial urine culture and to determine its correlation with the risk of postoperative urinary tract infection (UTI). Patients and methods We retrospectively identified all patients with preoperative polymicrobial urine culture in our center between January 2017 and October 2019. Preoperative urine cultures were collected 5 to 8 days before the surgery. No antibiotic prophylaxis was administered preoperatively in the absence of pyuria. Patients with pyuria (≥ 10 leukocytes/mm3) were treated preoperatively with Ceftriaxone. In case of beta-lactam allergy, the choice between other antibiotic therapies was left to the surgeon's discretion. A second urine culture was collected the day before surgery. The primary endpoint was the occurrence of UTI within 15 days following surgery. Results In all, 690 patients were included in the study. In line with our protocol, patients had Ceftriaxone, Fluoroquinolones, another antibiotic or no antibiotic prophylaxis in 492 cases (71.3%), 22 cases (3.2%), 31 cases (4.5%), and 145 cases (21%), respectively. The overall sterilization rate of 40.4% was similar between each treatment arm (P = 0.54). Postoperative UTI occurred in 68 cases (10.5%). In multivariate analysis, a sterile urine culture the day before surgery was the only factor decreasing the risk of postoperative UTI (OR 0.39, 95%CI, 0.17–0.84; P = 0.022). Conclusions Our findings suggest that empirical antibiotic therapy for the treatment of preoperative polymicrobial urine culture is no longer adequate. Further evaluation of organisms isolated may provide the necessary antibiograms for initiation of susceptibility based antibiotic therapy that could decrease postoperative UTI rates. Level of evidence 3.
- Published
- 2022
17. Comparative assessment of immunochromatography and ELISA diagnostic tests for HBsAg detection in PCR-confirmed HBV infection
- Author
-
Azita Navvabi, M.H. Khadem Ansari, F. Zitricky, H.R. Chalipa, and N. Navvabi
- Subjects
Male ,Hepatitis B virus ,medicine.medical_specialty ,HBsAg ,Saliva ,Enzyme-Linked Immunosorbent Assay ,Urine ,Iran ,Polymerase Chain Reaction ,Gastroenterology ,Chromatography, Affinity ,Pcr test ,Internal medicine ,medicine ,Humans ,Feces ,Hepatitis B Surface Antigens ,Diagnostic Tests, Routine ,business.industry ,Infant, Newborn ,Diagnostic test ,General Medicine ,Hepatitis B ,medicine.disease ,Elisa test ,Female ,Viral hepatitis ,business - Abstract
Introduction and aims Viral hepatitis, which appears most frequently at birth or during childhood, is a disease whose transmission routes include tears, bile, sexual fluids, sweat, milk, urine, feces, and saliva. The aim of the present study was to analyze the specificity of the immunochromatographic and ELISA diagnostic tests for hepatitis B surface antigen and compare them with PCR testing. Materials and methods The study sample was made up of 140 men and 60 women referred to the Urmia Medical University hospital to undergo PCR testing for HBV diagnosis. The ELISA test was performed using the Pioneer Medicine Company kit (Tehran, Iran). Results The results of the HBs-Ag rapid test and the ELISA test were compared with the PCR test. The HBs-Ag rapid test had 97% sensitivity and 91% specificity, whereas the ELISA test had 78% sensitivity and 76% specificity. Discussion and conclusion According to our results, the immunochromatographic test was accurate for diagnosing HBs-Ag in blood and the ELISA test had acceptable sensitivity and specificity, compared with PCR testing.
- Published
- 2022
18. Clinical utility of urinary soluble CD163 in evaluation of lupus nephritis patients
- Author
-
Eman R. Badawy, Mona H. Abd Elsamea, Esraa Talaat, Nada M. Gamal, and Hamdy M. Ibrahim
- Subjects
medicine.medical_specialty ,business.industry ,Urinary system ,Disease duration ,Urinary biomarkers ,Lupus nephritis ,Mean age ,Urine ,Renal SLEDAI ,RC581-607 ,medicine.disease ,Gastroenterology ,Urinary soluble CD163 ,Rheumatology ,Potential biomarkers ,Internal medicine ,medicine ,M2 macrophages ,Soluble cd163 ,Immunologic diseases. Allergy ,business ,skin and connective tissue diseases ,Renal biopsy ,Pathological - Abstract
Aim of the work: To assess urinary soluble CD163 (sCD136) in systemic lupus erythematosus (SLE) patients compared to healthy controls. In addition to determine its association with different SLE clinical features, laboratory investigations and pathological indices focusing on those suggest renal disease activity. Patients and methods: The study included 58 SLE patients and 30 controls. SLE disease activity index (SLEDAI) was assessed and patients subdivided into active lupus nephritis (ALN) (renal SLEDAI ≥ 4) and no-renal activity (NRA) SLE patients (renal SLEDAI = 0). Urinary sCD163 was measured by Enzyme-Linked Immunosorbent Assay (ELISA). Urine values were normalized to urinary creatinine excretion. Renal biopsies were performed in 21 ALN patients. Results: They were 54 females and 4 males with a mean age 31.8 ± 9.1 years and disease duration 6.2 ± 4.8 years. They were 31 with ALN and 27 NRA SLE patients. Urinary sCD163 level was significantly higher in SLE patients (1.85 ± 0.3) than controls (0.5 ± 0.36, p 0.82 with sensitivity of 90.3%, specificity of 88.89%, p
- Published
- 2022
19. Subtypes in patients with opioid misuse: A prognostic enrichment strategy using electronic health record data in hospitalized patients
- Author
-
Meng Xie, Brihat Sharma, Majid Afshar, Niranjan S. Karnik, Robert Kania, Cara Joyce, Elizabeth Salisbury-Afshar, Dmitriy Dligach, and Kristin Swope
- Subjects
Male ,Hospitalized patients ,Physiology ,Urine ,Machine Learning ,Tertiary Care Centers ,0302 clinical medicine ,Medicine and Health Sciences ,Electronic Health Records ,Public and Occupational Health ,030212 general & internal medicine ,Precision Medicine ,Uncategorized ,Analgesics ,Multidisciplinary ,Pharmaceutics ,Drugs ,Middle Aged ,Prognosis ,Latent class model ,Socioeconomic Aspects of Health ,Patient Discharge ,3. Good health ,Body Fluids ,Analgesics, Opioid ,Hospitalization ,Alcoholism ,Treatment Outcome ,Research Design ,Latent Class Analysis ,Cohort ,Medicine ,Female ,Anatomy ,medicine.drug ,Research Article ,Adult ,medicine.medical_specialty ,Census ,Drug Administration ,Science ,Research and Analysis Methods ,03 medical and health sciences ,Young Adult ,Drug Therapy ,Electronic health record ,Internal medicine ,Mental Health and Psychiatry ,medicine ,Pain Management ,Humans ,Socioeconomic status ,Prescription Drug Misuse ,Natural Language Processing ,Pharmacology ,Drug Screening ,Inpatients ,Survey Research ,Descriptive statistics ,business.industry ,Biology and Life Sciences ,Models, Theoretical ,Opioid-Related Disorders ,Opioids ,Health Care ,Opioid ,Observational study ,business ,030217 neurology & neurosurgery - Abstract
BackgroundApproaches are needed to better delineate the continuum of opioid misuse that occurs in hospitalized patients. A prognostic enrichment strategy with latent class analysis (LCA) may facilitate treatment strategies in subtypes of opioid misuse. We aim to identify subtypes of patients with opioid misuse and examine the distinctions between the subtypes by examining patient characteristics, topic models from clinical notes, and clinical outcomes.MethodsThis was an observational study of inpatient hospitalizations at a tertiary care center between 2007 and 2017. Patients with opioid misuse were identified using an operational definition applied to all inpatient encounters. LCA with eight class-defining variables from the electronic health record (EHR) was applied to identify subtypes in the cohort of patients with opioid misuse. Comparisons between subtypes were made using the following approaches: (1) descriptive statistics on patient characteristics and healthcare utilization using EHR data and census-level data; (2) topic models with natural language processing (NLP) from clinical notes; (3) association with hospital outcomes.FindingsThe analysis cohort was 6,224 (2.7% of all hospitalizations) patient encounters with opioid misuse with a data corpus of 422,147 clinical notes. LCA identified four subtypes with differing patient characteristics, topics from the clinical notes, and hospital outcomes. Class 1 was categorized by high hospital utilization with known opioid-related conditions (36.5%); Class 2 included patients with illicit use, low socioeconomic status, and psychoses (12.8%); Class 3 contained patients with alcohol use disorders with complications (39.2%); and class 4 consisted of those with low hospital utilization and incidental opioid misuse (11.5%). The following hospital outcomes were the highest for each subtype when compared against the other subtypes: readmission for class 1 (13.9% vs. 10.5%, pConclusionsA 4-class latent model was the most parsimonious model that defined clinically interpretable and relevant subtypes for opioid misuse. Distinct subtypes were delineated after examining multiple domains of EHR data and applying methods in artificial intelligence. The approach with LCA and readily available class-defining substance use variables from the EHR may be applied as a prognostic enrichment strategy for targeted interventions.
- Published
- 2023
- Full Text
- View/download PDF
20. Assessment of carnitine excretion and its ratio to plasma free carnitine as a biomarker for primary carnitine deficiency in newborns
- Author
-
Loek L. Crefcoeur, M. Rebecca Heiner‐Fokkema, Rose E. Maase, Gepke Visser, Monique G. M. de Sain‐van der Velden, and Paediatric Metabolic Diseases
- Subjects
ratio ,newborn screening ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,carnitine ,FCE ,PCD ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,plasma ,urine - Abstract
In the Netherlands, newborns are referred by the newborn screening (NBS) Program when a low free carnitine (C0) concentration (
- Published
- 2023
21. Antimicrobial Susceptibility Pattern of Bacterial Isolates from Urine Samples from Female Patients Suffering From Urinary Tract Infection at Tertiary Care Center in South India
- Author
-
null Vidya KR, Meghana Manjunath, null Lohit K, and null Prashanth HV
- Subjects
medicine.medical_specialty ,Epidemiology ,business.industry ,Urinary system ,Internal medicine ,Female patient ,Public Health, Environmental and Occupational Health ,Medicine (miscellaneous) ,Medicine ,Antimicrobial susceptibility ,Urine ,business ,Tertiary care - Abstract
Introduction: The frequency of bacteria affecting urinary tract and their antibiotic susceptibility pattern vary from one territory to another. This study was conducted to determine spectrum of bacterial isolates causing UTI and their antibiotic susceptibility pattern among female patients attending tertiary care center Materials and Methods: A total of 577 female urine culture sensitivity reports (CS) were collected. The culture was done by inoculation by standard loop technique. Antibiotic susceptibility testing for identified bacteria was done by Kirby-Bauer disc diffusion method and result was interpreted as per CLSI guidelines. Data was analyzed using SPSS software. Results: Infection rates were similar up to reproductive age group with no significant difference between gram-positive (GP) and gram-negative organisms (GN) but post reproductive age group suffered more from GN. The most commonly infecting organism was Staphylococci followed by Escherichia coli showing resistance in >50% tests to cephalosporins, penicillin and fluoroquinolones. All antibiotics are showing resistance from one or the other organisms. Conclusion: The commonly infecting bacteria are developing resistance to regularly used antibiotics raising concerns of cross resistance and selection of antibiotics. This warrants due diligence by all stakeholders, including public-health authorities, health care providers, policymakers and even the public to prevent and control antimicrobial resistance.
- Published
- 2022
22. The Effect of Dietary Phosphorus Restriction on Urine Protein Excretion in Patients With Proteinuria: A Randomized Controlled Trial
- Author
-
Alireza Soleimani, Negar Mozaffari-Rad, Nasrin Sharifi, and Hosein Akbari
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,030232 urology & nephrology ,Medicine (miscellaneous) ,Renal function ,chemistry.chemical_element ,Urine ,Gastroenterology ,law.invention ,Excretion ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Internal medicine ,medicine ,Humans ,Renal Insufficiency, Chronic ,Aged ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Proteinuria ,business.industry ,Phosphorus ,Middle Aged ,chemistry ,Nephrology ,Creatinine ,Phosphorus, Dietary ,Female ,medicine.symptom ,business ,Body mass index ,Dietary Phosphorus - Abstract
Objectives The present study was designed to determine the effect of dietary phosphorus restriction, independent of protein intake, on the urinary protein excretion in patients with proteinuria. Methods Seventy-one patients with proteinuria were enrolled in a parallel randomized controlled trial study. The patients were randomly allocated to receive either a recommended phosphorus-restricted diet (n = 36) or a recommended control diet (n = 35), for 8 weeks. A diet was designed and recommended to participants in a way that both trial groups would receive the same amount of energy and protein and the only significant difference between them was the amount of phosphorus intake. The study outcomes included the changes in spot urine protein-to-creatinine ratio, the changes in serum and urine levels of phosphorus, as well as the changes in estimated glomerular filtration rate (eGFR). Results The mean ± standard deviation of age, body mass index, and eGFR of the participants were 59 ± 14 years, 29 ± 5.5 kg/m2, and 56.1 ± 21.7 mL/min/1.73 m2, respectively. The amount of phosphorus intake decreased significantly in the phosphorus-restricted group compared to the control one (−709 vs. −369 mg/day; P Conclusions Although adherence to a phosphorus-restricted diet by patients with proteinuria led to a significant decrease in urinary protein excretion, this change was not significantly different from that of the control diet. Further studies with larger sample sizes and different designs will reveal more evidence for a link between phosphorus intake and proteinuria.
- Published
- 2022
23. Risk factors for the emergence of multidrug-resistant organisms in liver cirrhosis
- Author
-
Rita Carvalho, Alexandra Martins, Gonçalo Alexandrino, Maria Ana Rafael, Mariana Costa, Luísa Martins Figueiredo, and Joana Carvalho e Branco
- Subjects
Liver Cirrhosis ,Male ,medicine.medical_specialty ,Time Factors ,Cirrhosis ,medicine.drug_class ,Antibiotics ,Urine ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Drug Resistance, Multiple, Bacterial ,Internal medicine ,medicine ,Ascitic Fluid ,Humans ,In patient ,Prospective Studies ,Medical prescription ,Prospective cohort study ,Cross Infection ,Hepatology ,business.industry ,Gastroenterology ,Proton Pump Inhibitors ,General Medicine ,Bacterial Infections ,Length of Stay ,Middle Aged ,medicine.disease ,Patient Discharge ,Anti-Bacterial Agents ,Multiple drug resistance ,030220 oncology & carcinogenesis ,Cohort ,Female ,030211 gastroenterology & hepatology ,business - Abstract
Background Multidrug-resistant organisms (MDROs) are a reality that can alter the paradigm of treatment and prevention of infection in patients with liver cirrhosis (LC). Objective Identify risk factors for the occurrence of MDROs in patients with LC. Patients and methods Prospective study from October 2017 to March 2018 in consecutively hospitalized patients with decompensated LC with infection. Blood, urine and ascitic fluid cultures were analyzed. A p-value ≤0.05 was considered statistically significant. Results MDROs isolated in 18 of 52 episodes of infection. MDROs were associated with the use of proton pump inhibitors (PPIs) (p = 0.0312), antibiotic therapy in the last 90 days (p = 0.0033) and discharge within preceding 30 days or current hospitalization above 48 h (p = 0.0082). There was higher 90-day mortality in patients with MDROs infection (71.4% versus 35.7%, p = 0.0316). Conclusion MDROs infections were prevalent in this cohort and associated with 90-day mortality. Use of PPIs and antibiotics increased the risk of MDROs infections, suggesting that its prescription should be restricted to formal indication. Hospitalization was associated with the onset of MDROs, so LC patients should stay at the hospital the least possible. It is relevant to investigate other factors predisposing to the emergence of these microorganisms, in order to prevent it.
- Published
- 2022
24. Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles
- Author
-
Joseph F. Petrosino, Joseph W. McQuaid, Ali Hashemi Gheinani, Kohei Hasegawa, Shannon E. DiMartino, Rosalyn M. Adam, Kylie H. Davis, Candace Y. Chung, Jonathan M. Mansbach, Dijana Vitko, and Richard S. Lee
- Subjects
Male ,medicine.medical_specialty ,Fever ,Urology ,Urinary system ,Urine ,Gut flora ,urologic and male genital diseases ,Gastroenterology ,Vesicoureteral reflux ,Internal medicine ,medicine ,Prevotella ,Metabolome ,Humans ,Microbiome ,Vesico-Ureteral Reflux ,Kidney ,biology ,business.industry ,Microbiota ,Infant ,biology.organism_classification ,medicine.disease ,female genital diseases and pregnancy complications ,medicine.anatomical_structure ,Urinary Tract Infections ,Female ,business - Abstract
Children with vesicoureteral reflux (VUR) are at an increased risk of recurrent urinary tract infections (UTIs) and renal scarring. Gut microbiota are associated with disease phenotypes, but there has been no study that associates urinary microbiota (uMB) and metabolic profiles with VUR pathology. To identify dominant uMB genera and metabolites associated with UTIs in VUR, urine samples collected under sterile conditions underwent 16S ribosomal RNA sequencing (n = 49) and metabolomic analysis by mass spectrometry (n = 96). Alterations in uMB and metabolomic profiles in VUR patients suggest remodeling of urinary bacterial communities after UTIs: Dorea- and Escherichia-dominant uMB profiles were more frequently identified in participants with VUR. Prevotella- and Lactobacillus-dominant uMB profiles were more prevalent in controls (p 0.001). Microbial composition varied based on recurrent febrile UTI status (p = 0.001). A total of 243 urinary metabolites involved in energy, amino acid, nucleotide, and lipid metabolism were altered in VUR patients with UTIs (p 0.05). Importantly, VUR specimens revealed changes in the bacteria-associated metabolic pathways such as glutamate degradation, methyl-citrate cycle, and bile acid metabolism. PATIENT SUMMARY: Differences in urinary commensal bacteria and metabolites exist between children with and without vesicoureteral reflux (VUR). These changes may be utilized to identify patients at risk of VUR-associated kidney damage.
- Published
- 2022
25. Low-Iodine Diet of 4 Days Is Sufficient Preparation for I-131 Therapy in Differentiated Thyroid Cancer Patients
- Author
-
Judith A P Bons, Adrienne H. Brouwers, Mirthe H Links, Linda G Swart-Busscher, Anneke C. Muller Kobold, Bernadette L Dekker, Thera P. Links, Marleen Kars, Anouk N A van der Horst-Schrivers, Interne Geneeskunde, MUMC+: MA Endocrinologie (9), MUMC+: DA CDL Algemeen (9), RS: NUTRIM - R3 - Respiratory & Age-related Health, Guided Treatment in Optimal Selected Cancer Patients (GUTS), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE), and Damage and Repair in Cancer Development and Cancer Treatment (DARE)
- Subjects
Male ,Calorie ,SYMPORTER ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Urine ,REEVALUATION ,Biochemistry ,Gastroenterology ,ABLATION THERAPY ,Iodine Radioisotopes ,Endocrinology ,low-iodine diet ,ASSOCIATION GUIDELINES ,Prospective Studies ,Prospective cohort study ,Thyroid cancer ,individual perceptions ,urinary iodine excretion ,OUTPATIENT PREPARATION ,Radioiodine therapy ,Middle Aged ,radioactive iodine therapy ,Diet Records ,iodine intake ,EXPERIENCES ,Female ,AcademicSubjects/MED00250 ,Iodine ,Adult ,medicine.medical_specialty ,nutrition diary ,chemistry.chemical_element ,Nutritional Status ,Context (language use) ,Excretion ,SEVERE HYPONATREMIA ,Internal medicine ,medicine ,MANAGEMENT ,Humans ,Thyroid Neoplasms ,Online Only Articles ,Clinical Research Articles ,Aged ,business.industry ,Biochemistry (medical) ,medicine.disease ,Diet ,Trace Elements ,chemistry ,business - Abstract
Context No consensus exists about the optimal duration of the low-iodine diet (LID) in the preparation of 131I therapy in differentiated thyroid cancer (DTC) patients. Objective This work aimed to investigate if a LID of 4 days is enough to achieve adequate iodine depletion in preparation for 131I therapy. In addition, the nutritional status of the LID was evaluated. Methods In this prospective study, 65 DTC patients treated at 2 university medical centers were included between 2018 and 2021. The patients collected 24-hour urine on days 4 and 7 of the LID and kept a food diary before and during the LID. The primary outcome was the difference between the 24-hour urinary iodine excretion (UIE) on both days. Results The median 24-hour UIE on days 4 and 7 of the LID were not significantly different (36.1 mcg [interquartile range, 25.4-51.2 mcg] and 36.5 mcg [interquartile range, 23.9-47.7 mcg], respectively, P = .43). On day 4 of the LID, 72.1% of the DTC patients were adequately prepared (24-hour UIE Conclusion The 24-hour UIE on day 4 of the LID did not differ from day 7, and therefore shortening the LID from 7 to 4 days seems justified to prepare DTC patients for 131I therapy in areas with sufficient iodine intake and may be beneficial to maintain a sufficient nutritional intake during DTC treatment.
- Published
- 2022
26. Инфекция мочевой системы у детей с нейрогенной дисфункцией мочевого пузыря
- Author
-
T.A. Morozova and Yu.B. Bielan
- Subjects
medicine.medical_specialty ,biology ,medicine.drug_class ,business.industry ,Urinary system ,Antibiotics ,Congenital cytomegalovirus infection ,Chlamydiae ,General Medicine ,Urine ,medicine.disease_cause ,medicine.disease ,biology.organism_classification ,Gastroenterology ,Internal medicine ,medicine ,business ,Mycoplasma genitalium ,Neurogenic bladder dysfunction ,Ureaplasma urealyticum - Abstract
Актуальность. Среди всех заболеваний мочевой системы доминируют микробно-воспалительные поражения, распространенность которых составляет 29 на 1000 человек детского населения. Нейрогенная дисфункция мочевого пузыря приводит к хронизации и рецидивированию инфекций мочевой системы. Цель исследования. Изучение этиологического спектра у детей с инфекцией мочевой системы в сочетании с нейрогенной дисфункцией мочевого пузыря. Материалы и методы. Проведено обследование 434 детей в возрасте от 4 до 15 лет, находившихся на стационарном и амбулаторном лечении в городе Омске с 2008 по 2014 год. Осуществлен бактериологический посев мочи на микрофлору с определением чувствительности к антибактериальным препаратам с использованием Urin System Plus (Liofilchem, Италия). Параллельно исследовалась моча на наличие Cytomegalovirus, Herpes simplex virus I–II, Mycoplasma genitalium, Chlamydiae trachomatis, Ureaplasma urealyticum с помощью метода полимеразной цепной реакции и иммуноферментного анализа. Обработка данных проводилась с использованием пакета программ Statistica 10.0. Результаты. Установлено, что у детей с инфекциями мочевой системы и нейрогенной дисфункцией мочевого пузыря в микробном пейзаже мочи доминируют представители семейства Staphylococcus в отличие от спектра уропатогенов у пациентов с инфекцией мочевой системы без функциональной обструкции, для которых характерно преобладание Escherichia coli. Высокая частота обнаружения цитомегаловирусов в моче свидетельствует о значительном снижении иммунитета у детей с инфекциями мочевой системы и нейрогенной дисфункцией мочевого пузыря. Заключение. Показанные отличия в микробном пейзаже мочи при инфекции мочевой системы в сочетании с нейрогенной дисфункцией мочевого пузыря и без функциональной обструкции необходимо учитывать при подборе адекватной терапии пациентам разных групп, включая иммунокорригирующие подходы.
- Published
- 2022
27. A comparative study of smica in various body fluids of diagnosed cervical cancer patients and healthy women
- Author
-
SomshekharPatil, Meghnad Joshi, Pooja A.Pachani, Amita Gosavi, Rakesh k Sharma, Jeevitaa Kshersagar, Rajendra R. Godbole, and Rakhi Jagdale
- Subjects
papanicolaou test ,Saliva ,medicine.medical_specialty ,Urine ,Gastroenterology ,Metastasis ,uterine cervical neoplasms ,Internal medicine ,neoplastic processes ,medicine ,Cervical cancer ,medicine.diagnostic_test ,business.industry ,Disease progression ,Obstetrics and Gynecology ,Cancer ,Gynecology and obstetrics ,Gynecologic Oncology ,medicine.disease ,parity ,Immunoassay ,RG1-991 ,Original Article ,Histopathology ,body fluids ,business - Abstract
Objective Cervical cancer (CC) is a major public health problem in women, and its early detection can help reduce morbidity and mortality. The objective of this study was to compare serum levels of soluble major histocompatibility complex class I-related chain A (sMICA) levels in various body fluids between women diagnosed with CC and healthy women.Methods A case-control study was conducted at a tertiary care hospital and a cancer center in Kolhapur, India. Overall, 150 individuals (100 CC patients and 50 healthy women) participated after providing informed written consent. Demographic data, histopathology history, parity, and tumor, node, and metastasis (TNM) staging data were collected. Pap smears, saliva, blood, and urine samples were collected. Pap smears were examined microscopically, and sMICA levels in all samples were determined by enzyme-linked immunoassay (ELISA).Results The mean age of women with cervical cancer was 49.86±8.18 years. Squamous cell carcinoma (70%) was the most common histological variant in CC patients. Serum soluble sMICA levels differed significantly with parity and TNM staging (P
- Published
- 2022
28. Detection of testosterone microdosing in healthy females
- Author
-
David J. Handelsman, Vinod Nair, Sasha Savkovic, Lam P Ly, Reena Desai, John Howa, and Daniel Eichner
- Subjects
medicine.medical_specialty ,medicine.drug_class ,Pharmaceutical Science ,Mean corpuscular hemoglobin ,Urine ,Analytical Chemistry ,Reticulocyte ,Internal medicine ,medicine ,Humans ,Environmental Chemistry ,Testosterone ,Adverse effect ,Spectroscopy ,Doping in Sports ,Hematology ,medicine.diagnostic_test ,business.industry ,Epitestosterone ,Dihydrotestosterone ,Androgen ,medicine.anatomical_structure ,Endocrinology ,Androgens ,Female ,Steroids ,business ,medicine.drug - Abstract
The ready detectability of synthetic androgens by mass spectrometry (MS)-based antidoping tests has reoriented androgen doping to using testosterone (T), which must be distinguished from its endogenous counterpart making detection of exogenous T harder. We investigated urine and serum steroid and hematological profiling individually and combined to determine the optimal detection model for T administration in women. Twelve healthy females provided six paired blood and urine samples over 2 weeks prior to treatment consisting of 12.5-mg T in a topical transdermal gel applied daily for 7 days. Paired blood and urine samples were then obtained at the end of treatment and Days 1, 2, 4, 7, and 14 days later. Compliance with treatment and sampling was high, and no adverse effects were reported. T treatment significantly increased serum and urine T, serum dihydrotestosterone (DHT), urine 5α-androstane-3α,17β-diol (5α-diol) epitestosterone (E), and urine T/E ratio with a brief window of detection (2-4 days) as well as total and immature (medium and high fluorescence) reticulocytes that remained elevated over the full 14 posttreatment days. Carbon isotope ratio MS and the OFF score and Abnormal Blood Profile score (ABPS) were not discriminatory. The optimal multivariate model to identify T exposure combined serum T, urine T/E ratio with three hematological variables (% high fluorescence reticulocytes, mean corpuscular hemoglobin, and volume) with the five variables providing 93% correct classification (4% false positive, 10% false negatives). Hence, combining select serum and urine steroid MS variables with reticulocyte measures can achieve a high but imperfect detection of T administration to healthy females.
- Published
- 2022
29. The metabolism and excretion of the dipeptidyl peptidase 4 inhibitor [14C] cetagliptin in healthy volunteers
- Author
-
Zheming Gu, Zhenwen Yu, Tong Wang, Yating Xiong, Xusheng Tian, Hua Zhang, Jinsong Ding, Qiang Yu, Jinmiao Lu, Zengyan Xu, Juping Ding, Xinyi Zhou, Yicong Bian, and Dong Tang
- Subjects
Pharmacology ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Metabolite ,General Medicine ,Urine ,Dipeptidyl peptidase-4 inhibitor ,Metabolism ,Toxicology ,Glucuronic acid ,Biochemistry ,Excretion ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Oral administration ,Internal medicine ,medicine ,Feces ,medicine.drug - Abstract
1. The metabolism and excretion of cetagliptin were investigated in healthy male subjects after a single oral dose of 100mg/50μCi [14C] cetagliptin.2. The mean concentration-time profile of cetagliptin was similar to that of total radioactivity in plasma after oral administration of [14C] cetagliptin in healthy male subjects. Cetagliptin was rapidly absorbed after oral administration. Unchanged cetagliptin was the most abundant radioactive component in all matrices investigated. Approximately 53.13% of plasma AUC of total radioactivity was accounted for by cetagliptin. Each metabolite plasma AUC was not higher than 2.93% of plasma AUC of total radioactivity. By 336 h after administration, 91.68% of the administered radioactivity was excreted, and the cumulative excretion in the urine and feces was 72.88% and 18.81%, respectively. The primary route of excretion of radioactivity was via the kidneys.3. Four metabolites were detected at trace levels, and it involved hydroxylated (M436-1 and M436-3), N- sulfate (M500), and N-carbamoyl glucuronic acid conjugates (M640B) of cetagliptin. These metabolites were detected also in plasma, urine, and feces at low levels, except that metabolite M640B was not detected in feces. All metabolites were observed with < 10% of parent compound systemic exposure after oral administration.
- Published
- 2022
30. Asian guidelines for UTIs & STIs UTI section: Complicated UTIs with neurogenic bladder
- Author
-
Lap Yin Ho, Christopher K C Lai, Stanley Chi Fai Kan, Raymond Wai Man Kan, Alex Hak-Keung Lo, and Bill T.H. Wong
- Subjects
Microbiology (medical) ,medicine.medical_specialty ,Bacteriuria ,medicine.drug_class ,Urinary system ,Antibiotics ,Sexually Transmitted Diseases ,Urine ,urologic and male genital diseases ,Antibiotic resistance ,Internal medicine ,Epidemiology ,Humans ,Medicine ,Pharmacology (medical) ,In patient ,Urinary Bladder, Neurogenic ,Antibiotic prophylaxis ,Bladder drainage ,business.industry ,bacterial infections and mycoses ,female genital diseases and pregnancy complications ,Anti-Bacterial Agents ,Infectious Diseases ,Urinary Tract Infections ,business - Abstract
Summary Epidemiology and pathogenesis Urinary tract infection (UTI) in patients with neurogenic bladder causes significant morbidity and mortality. Diagnosis UTI in neurogenic bladder causes atypical symptomatology. Urine tests are pivotal in confirming or excluding UTI, and in guiding appropriate antibiotic treatment. Treatment 1. Symptomatic UTI warrants appropriate antibiotic treatment with reference to culture results and local antibiotic resistance patterns. Asymptomatic bacteriuria should not be treated, and antibiotic prophylaxis is generally not recommended.2. Adequate bladder drainage is essential in reducing the occurrence of urinary tract infections.3. Recurrent UTI in neurogenic bladder may necessitate the treatment of neurogenic detrusor overactivity and the restoration of low bladder pressure during bladder storage and voiding by drugs or surgery.
- Published
- 2022
31. Increased Collagen Turnover Is a Feature of Fibromuscular Dysplasia and Associated With Hypertrophic Radial Remodeling: A Pilot, Urine Proteomic Study
- Author
-
Hakim Khettab, Agnieszka Latosinska, Harald Mischak, Jan A. Staessen, Alessandra Bacca, Christophe Beauloye, Stefano Taddei, Pierre Boutouyrie, Miikka Vikkula, Alexandre Persu, Rosa Maria Bruno, Marco Pappaccogli, Laurent Toubiana, Laboratoire d'Informatique Médicale et Ingénierie des Connaissances en e-Santé (LIMICS), Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), UCL - SSS/DDUV/GEHU - Génétique, and UCL - (SLuc) Service de cardiologie
- Subjects
Adult ,Male ,Proteomics ,collagen ,kidney ,Pathology ,medicine.medical_specialty ,hypertension ,Urinary system ,fibromuscular dysplasia ,Coronary Artery Disease ,Fibromuscular dysplasia ,Urine ,030204 cardiovascular system & hematology ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,Internal Medicine ,medicine ,Humans ,Renal Insufficiency, Chronic ,Aged ,030304 developmental biology ,0303 health sciences ,Kidney ,business.industry ,Middle Aged ,medicine.disease ,3. Good health ,medicine.anatomical_structure ,Feature (computer vision) ,Proteome ,Female ,[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] ,business - Abstract
Fibromuscular dysplasia (FMD), a nonatherosclerotic, noninflammatory disease of medium-sized arteries, is an underdiagnosed disease. We investigated the urinary proteome and developed a classifier for discrimination of FMD from healthy controls and other diseases. We further hypothesized that urinary proteomics biomarkers may be associated with alterations in medium-sized, but not large artery geometry and mechanics. The study included 33 patients with mostly multifocal, renal FMD who underwent in depth arterial exploration using ultra-high frequency ultrasound. The cohort was separated in a training set of 23 patients with FMD from Belgium and an independent test set of 10 patients with FMD from Italy. For each set, controls matched 2:1 were selected from the Human Urinary Proteome Database. The specificity of the classifier was tested in 700 additional controls from general population studies, patients with chronic kidney disease (n=66) and coronary artery disease (n=31). Three hundred thirty-five urinary peptides, mostly related to collagen turnover, were identified in the training cohort and combined into a classifier. When applying in the test cohort, the area under the receiver operating characteristic curve was 1.00, 100% specificity at 100% sensitivity. The classifier maintained a high specificity in additional controls (98.3%), patients with chronic kidney (90.9%) and coronary artery (96.8%) diseases. Furthermore, in patients with FMD, the proteomic score was positively associated with radial wall thickness and wall cross-sectional area. In conclusion, a proteomic score has the potential to discriminate between patients with FMD and controls. If confirmed in a wider and more diverse cohort, these findings may pave the way for a noninvasive diagnostic test of FMD.
- Published
- 2022
32. Urinary L-FABP is a promising prognostic biomarker of ACLF and mortality in patients with decompensated cirrhosis
- Author
-
Manuel Morales-Ruiz, Marta Carol, Wladimiro Jiménez, Octavi Bassegoda, Isabel Graupera, Ann T. Ma, Adrià Juanola, Salvatore Piano, Núria Fabrellas, Laia Escudé, Martina Pérez-Guasch, Elisa Pose, Pere Ginès, Marta Cervera, Cristina Solé, Gonzalo Crespo, Chiara Elia, Ferran Torres, Ana-Belén Rubio, Elsa Solà, Emma Avitabile, and Laura Napoleone
- Subjects
Male ,medicine.medical_specialty ,Cirrhosis ,Urinary system ,Kaplan-Meier Estimate ,Urine ,Fatty Acid-Binding Proteins ,Gastroenterology ,Statistics, Nonparametric ,L-FABP ,Internal medicine ,medicine ,Risk of mortality ,Humans ,Nonparametric ,Prospective Studies ,Mortality ,Acute tubular necrosis ,Aged ,Proportional Hazards Models ,Univariate analysis ,Hepatology ,business.industry ,Acute-On-Chronic Liver Failure ,Liver ,Organ Failure ,Biomarkers ,Female ,Middle Aged ,Multivariate Analysis ,Prognosis ,Statistics ,medicine.disease ,Cohort ,Biomarker (medicine) ,lipids (amino acids, peptides, and proteins) ,business - Abstract
Decompensated cirrhosis (DC) is associated with high mortality, mainly owing to the development of acute-on-chronic liver failure (ACLF). Identifying the patients with DC who are at high risk of mortality and ACLF development is an unmet clinical need. Liver fatty acid-binding protein (L-FABP) is expressed in several organs and correlates with liver and systemic inflammation. Herein, we aimed to assess the prognostic value of L-FABP in patients with DC.A prospective series of 444 patients hospitalized for DC was divided into 2 cohorts: study cohort (305 patients) and validation cohort (139 patients). L-FABP was measured in urine and plasma samples collected at admission. Neutrophil gelatinase-associated lipocalin (NGAL) was also measured in urine samples for comparison.Urine but not plasma L-FABP correlated with 3-month survival on univariate analysis. On multivariate analysis, urine L-FABP and model for end-stage liver disease (MELD)-Na were the only independent predictors of prognosis. Urine L-FABP levels were higher in patients with ACLF than in those without and also predicted the development of ACLF, together with MELD-Na, during follow-up. In patients with ACLF, urine L-FABP correlated with liver, coagulation, and circulatory failure. Urine L-FABP levels were also increased in patients with acute kidney injury, particularly in those with acute tubular necrosis. The ability of urinary L-FABP to predict survival and ACLF development was confirmed in the validation cohort. Urine NGAL predicted outcome on univariate but not multivariate analysis.Urinary L-FABP levels are independently associated with the 3-month clinical course in patients with DC, in terms of mortality and ACLF development. Urinary L-FABP is a promising prognostic biomarker for patients with DC.Increased levels of liver fatty acid-binding protein (L-FABP), a protein related to lipid metabolism, have been associated with liver-related diseases. The present study analyzed urinary L-FABP levels in 2 independent groups of patients with decompensated cirrhosis and showed that higher urinary L-FABP levels correlated with increased mortality and risk of acute-on-chronic liver failure development. Therefore, urinary L-FABP levels could be useful as a new tool to predict complications in patients with decompensated cirrhosis.
- Published
- 2022
33. Orientation diagnostique devant une coloration anormale des urines : la roue à urines 2.0
- Author
-
Caroline Schmitt, Caroline Sayegh, J. Pouchot, Marie Aude Penet, Edouard Flamarion, Jean Baptise Arnoux, Antoine Poli, G. Cheminet, Romane Bariseel, Constance Reichert, David de Saint Gilles, and Alexandre Karras
- Subjects
medicine.medical_specialty ,Modern medicine ,Chyluria ,business.industry ,Myoglobinuria ,030232 urology & nephrology ,Gastroenterology ,Urine ,medicine.disease ,Dermatology ,Alkaptonuria ,03 medical and health sciences ,0302 clinical medicine ,Porphyria ,Purple urine bag syndrome ,Internal Medicine ,medicine ,Hemoglobinuria ,030212 general & internal medicine ,medicine.symptom ,business - Abstract
Looking at the urine for diagnostic purposes, once performed by ancient Egyptians, can still provide some valuable clues in modern medicine. Several diseases have been named after their associated urine color and this underlines the clinical value of visual urine inspection: blue diaper disease, purple urine bag syndrome, black urine disease or porphyria. Abnormal urine color could be challenging for the clinician: it may reveal neoplastic disease (urologic cancer; melanoma), cell lysis (rhabdomyolysis; hemolysis), infection (lymphatic filariasis; malaria), enzyme deficiency (porphyria; alkaptonuria), medication or food intake. In this article, we present the diagnostic approach, the mechanisms involved and the main causes of abnormal urine color.
- Published
- 2022
34. Peripheral blood lymphocytes are able to maintain their viability and basic function in normal urine
- Author
-
Azin Aghamajidi, Hesam Babaei khameneh, Narges Amirjamshidi, Zeinab Abedian, Hamidreza Khorasani, and Amrollah Mostafazadeh
- Subjects
urine ,lymphocyte ,viability ,Internal medicine ,RC31-1245 - Abstract
Background: Similar to inflammatory cells, peripheral blood mononuclear cells (PBMCs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. In this study we determined the viability rate and response to phytohemagglutinin-A (PHA) of human PBMCs in normal urine. Methods: A number of 1×106 ficoll-hypaque isolated PBMCs were dispensed in 1 ml normal urine and 6 molar urea and RPMI-1640+FBS10 % were considered as negative and positive control, respectively. After 20, 60 and 120 minutes the viability of these cells was measured by trypan blue dye exclusion assay. 1×105 of PBMCs were isolated from urine and cultured as triplicate in RPMI-1640`supplemented with FBS 10% and PHA for 96hr. MTT assay was performed to determine the PBMCs response to PHA. These experiments were repeated three times independently. Results: There was no significant difference between the viability rates of the PBMCs incubated in urine and positive control after 20, 60 and 120 minutes while after 60 minutes they exhibited 75.6% of reactivity to PHA versus positive control. Overall, there was a significant difference in trends of viability rate across the three groups (p
- Published
- 2016
35. Association between hyperuricemia and clinical pathological characteristics of patients with IgA nephropathy
- Author
-
Ling Lu, Hua-Guo Feng, Guo-Hua Dai, Yi Feng, and Bin Li
- Subjects
medicine.medical_specialty ,Blood lipids ,Renal function ,Hyperuricemia ,Urine ,Kidney ,urologic and male genital diseases ,Gastroenterology ,Nephropathy ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,Creatinine ,business.industry ,nutritional and metabolic diseases ,Glomerulonephritis, IGA ,General Medicine ,medicine.disease ,Uric Acid ,Blood pressure ,chemistry ,Uric acid ,Liver function ,business - Abstract
OBJECTIVE To study the association of hyperuricaemia with clinical and pathological characteristics of patients with IgA nephropathy, and to clarify adverse effects of hyperuricaemia on the onset and progression of IgA nephropathy. METHODS A total of 244 patients with IgA nephropathy enrolled in Jiangjin Center Hospital were divided into a group with normal serum uric acid level and a group with elevated level. Age, gender, course of disease, blood pressure, liver function, renal function, blood lipid levels, blood glucose level, 24-hour urine protein level and pathological grades were recorded. The correlations of serum uric acid level with clinical indices and pathological grades were analyzed. RESULTS The incidence rate of IgA nephropathy complicated with hyperuricaemia was 25.4%. The two groups had significantly different course of disease, body mass index (BMI), and levels of urea nitrogen, creatinine, triglyceride and urine protein (p
- Published
- 2021
36. Urinary 11‐dehydrothromboxane<scp>B2</scp>concentrations in 20 dogs with primary immune‐mediated hemolytic anemia
- Author
-
Alison Ridyard, Neil P. Evans, and Elizabeth A Conway
- Subjects
Hemolytic anemia ,medicine.medical_specialty ,education.field_of_study ,General Veterinary ,business.industry ,Thromboxane ,Veterinary medicine ,Urinary system ,Population ,canine ,Urine ,thromboembolism ,medicine.disease ,survival ,Thrombosis ,Gastroenterology ,IMHA ,Internal medicine ,SF600-1100 ,Medicine ,business ,education ,11-dehydrothromboxane B2 ,thromboxane ,Immune mediated hemolytic anemia - Abstract
Background Thromboembolic disease is a major cause of mortality in dogs with immune‐mediated hemolytic anemia (IMHA). At present, no reliable biomarkers of individual patient thrombotic risk are available. In human medicine, increased urinary thromboxane concentrations have utility as markers of prothrombotic tendency in various situations. Hypothesis/Objectives First, to determine if urinary 11‐dehydrothromboxane B2 (u11‐dTXB) concentrations are increased in dogs with primary IMHA compared to normal dogs; second, to assess whether u11‐dTXB concentration is associated with survival, known prognostic indicators, or frequency of thrombosis in dogs with IMHA. Animals Twenty client‐owned dogs diagnosed with primary IMHA and 17 healthy dogs volunteered by hospital staff. Methods Prospective case‐control study. A previously validated ELISA was used to measure urine 11‐dTXB concentrations, which were normalized to urine creatinine concentration (u11‐dTXB:Cr). Samples were obtained at presentation from patients with primary IMHA. Standard clincopathological data at baseline and survival data were collected. Urinary 11‐dTXB:Cr was compared between outcome subgroups, and correlated with known markers of disease severity. Results Baseline u11‐dTXB:Cr was significantly higher in dogs with IMHA than in healthy dogs (median, 3.75; range, 0.83‐25.36 vs 0.65; 0.24‐2.57; P = .003) but did not differ between dogs with IMHA that survived and did not survive to 30 days after presentation, nor between dogs with and without clinical suspicion of thrombotic disease. Conclusions and Clinical Importance Urinary 11‐dTXB:Cr is increased in dogs with IMHA compared to healthy controls, consistent with a prothrombotic state. However, in this IMHA population u11‐dTXB:Cr was not associated with survival or suspected thrombosis.
- Published
- 2021
37. Clinical investigation of intestinal fatty acid-binding protein (I-FABP) as a biomarker of SARS-CoV-2 infection
- Author
-
Rafael Simone Saia, Karen Mirna Loro Morejón, Fábio Luis-Silva, Maria Auxiliadora-Martins, Márcia Regina Cadelca, Humberto Giusti, Anibal Basile-Filho, Augusto Marcussi Degiovani, and Karina Bonicenha Pedroso
- Subjects
ROC curve, receiver operating characteristic curve ,Cr, creatinine ,Infectious and parasitic diseases ,RC109-216 ,Urine ,TNF-α, tumor necrosis factor-α ,Gastroenterology ,RT-PCR, reverse transcriptase polymerase chain reaction ,COVID-19, coronavirus disease of 2019 ,IFN-γ, interferon-γ ,Prospective Studies ,Gastrointestinal tract ,biology ,ELISA, enzyme-linked immunosorbent assay ,General Medicine ,GI, gastrointestinal ,ICU, intensive care unit ,HIV, human immunodeficiency virus ,C-Reactive Protein ,Infectious Diseases ,CRP, C-reactive protein ,ACE-2, angiotensin converting enzyme-2 ,Biomarker (medicine) ,intestinal epithelial cells ,medicine.symptom ,Brazil ,Microbiology (medical) ,medicine.medical_specialty ,Short Communication ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Urinary system ,Inflammation ,AUC, area under curve ,Fatty Acid-Binding Proteins ,SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 ,Interferon-gamma ,Internal medicine ,medicine ,Humans ,INTERLEUCINA 6 ,IBD, inflammatory bowel diseases ,Interleukin 6 ,ARDS, acute respiratory distress syndrome ,Tropism ,business.industry ,interleukin-6 ,COVID-19 ,Ig, immunoglobulin ,cytokines ,IL, interleukin ,Enterocytes ,inflammation ,biology.protein ,business ,Biomarkers - Abstract
OBJECTIVES: SARS-CoV-2 exhibits tropism for the gastrointestinal tract, however lesions in enterocytes and their correlation with disease severity and patient prognosis are still unknown. METHODS: SARS-CoV-2 patients were enrolled in five medical centres in the Sao Paulo State, Brazil. Clinical characteristics and laboratory findings of patients were recorded. At admission, 7th and 14th day of hospitalisation, plasma and urine samples were collected. The levels of cytokines (IFN-I³, IL-6 and TNF-α) and the biomarker intestinal fatty acid-binding protein (I-FABP) were measured. RESULTS: COVID-19 patients displayed ≈48-, 74- and 125-fold increased urinary I-FABP levels at admission (n=283; p
- Published
- 2021
38. Use of Salivary Iodine Concentrations to Estimate the Iodine Status of Adults in Clinical Practice
- Author
-
Bernadette L Dekker, Anouk N A van der Horst-Schrivers, Anneke C. Muller Kobold, D.A. Janneke Dijck-Brouwer, Thera P. Links, Daan J Touw, Michel J. Vos, Pharmaceutical Analysis, Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Medicinal Chemistry and Bioanalysis (MCB), Groningen Research Institute for Asthma and COPD (GRIAC), Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Damage and Repair in Cancer Development and Cancer Treatment (DARE), MUMC+: CAKZ Medische afdeling SEH (9), and RS: FHML non-thematic output
- Subjects
Adult ,medicine.medical_specialty ,Saliva ,urinary iodine ,Nutrition and Disease ,SAMPLES ,Nutritional Status ,Medicine (miscellaneous) ,chemistry.chemical_element ,salivary protein ,Urine ,Iodine ,GUIDELINES ,Gastroenterology ,salivary urea ,DIET ,Excretion ,AcademicSubjects/MED00060 ,Internal medicine ,Humans ,Medicine ,Thyroid Neoplasms ,Thyroid cancer ,RADIOACTIVE IODINE ,DIFFERENTIATED THYROID-CANCER ,Nutrition and Dietetics ,business.industry ,medicine.disease ,iodine intake ,salivary iodine ,Clinical Practice ,chemistry ,Salivary Proteins ,AcademicSubjects/SCI00960 ,Urinary iodine ,business - Abstract
Background: Measurement of the 24-h urinary iodine concentration or urinary iodine excretion (UIE) is the gold standard to determine iodine status; however, this method is inconvenient. The use of salivary iodine could be a possible alternative since salivary glands express the sodium-iodine symporter.Objectives: We aimed to establish the correlation between the salivary iodine secretion and UIE, to evaluate the clinical applicability of the iodine saliva measurement.Methods: We collected 24-h urine and saliva samples from 40 participants >= 18 y: 20 healthy volunteers with no specific diet (group 1), 10 patients with differentiated thyroid cancer with a low dietary intake (
- Published
- 2021
39. Detection of KRAS mutations in circulating tumour DNA from plasma and urine of patients with colorectal cancer
- Author
-
Akihisa Matsuda, Seiichi Shinji, Goro Takahashi, Hiroshi Yoshida, Takuma Iwai, Toshimitsu Miyasaka, Yasuyuki Yokoyama, Sho Kuriyama, Takeshi Yamada, Hiromichi Sonoda, Keisuke Hara, Ryo Ohta, Kohki Takeda, and Koji Ueda
- Subjects
Adult ,Male ,medicine.medical_specialty ,Colorectal cancer ,Pleural effusion ,Urine ,medicine.disease_cause ,Gastroenterology ,Circulating Tumor DNA ,Proto-Oncogene Proteins p21(ras) ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Digital polymerase chain reaction ,Prospective Studies ,Liquid biopsy ,Aged ,Neoplasm Staging ,Aged, 80 and over ,business.industry ,Liquid Biopsy ,General Medicine ,Middle Aged ,medicine.disease ,Minimal residual disease ,Oncology ,Mutation ,Sputum ,Female ,Surgery ,KRAS ,medicine.symptom ,Colorectal Neoplasms ,business - Abstract
Background Circulating tumour DNA (ctDNA) is very useful for purposes of cancer genetics; however, it has some limitations. Recently, ctDNA in body fluids, such as urine, sputum, and pleural effusion, has been investigated. The aim of this study was to evaluate the quantity of ctDNA derived from urine (trans-renal ctDNA) and the accuracy of KRAS mutation detection in relation to disease stage in colorectal cancer. Methods Urine, plasma, and tissue samples were collected from consecutively resected colorectal cancer patients. DNA was extracted from each sample and the quantity was determined. From each DNA sample, KRAS mutations were detected using droplet digital PCR. Results 200 patients participated and KRAS mutations were detected in 84 patients (42.0%) from tumour tissue. The concentration of trans-renal ctDNA (trtDNA) was significantly lower than that of plasma; however, there was no significant difference between the sensitivity using ctDNA and that using trtDNA (29.8% VS 33.3%, p = 0.62). Concordance between these two tests was only 17.5%. Combination analysis (ctDNA + trtDNA) improved the sensitivity to 53.6%, and sensitivity was significantly higher than that of corresponding single assays (p = 0.003). In early cancer stages, trtDNA had greater sensitivity for detecting KRAS mutations than ctDNA (37.7% vs. 21.3%, p = 0.047). Conversely, it was less useful for advanced cancer stages (21.7% vs. 52.2%, p = 0.07). Notably, KRAS mutations were detected using ctDNA or trtDNA in 12 of 116 (10.3%) patients who had no KRAS mutations in their tissue samples. Conclusions: trtDNA and ctDNA have equal potential and combination analysis significantly improved the sensitivity.
- Published
- 2021
40. Female nurses have a higher prevalence of urinary tract symptoms and infection than other occupations in dialysis units
- Author
-
Fabiana B Nerbass, Cintia E Santo, Edilaine V Fialek, Viviane Calice-Silva, and Marcos A Vieira
- Subjects
Adult ,Nephrology ,medicine.medical_specialty ,Urinary urgency ,medicine.medical_treatment ,Urinary system ,Population ,030232 urology & nephrology ,Psychological intervention ,Nurses ,Hydration ,Urine ,Occupational safety and health ,03 medical and health sciences ,0302 clinical medicine ,Lower Urinary Tract Symptoms ,Renal Dialysis ,Internal medicine ,Prevalence ,medicine ,Humans ,030212 general & internal medicine ,Occupations ,Urinary Tract ,education ,Toilet Behavior ,Occupational Health ,Dialysis ,education.field_of_study ,business.industry ,General Medicine ,Middle Aged ,Urinary Tract Symptom ,Diseases of the genitourinary system. Urology ,Urinary Tract Infection ,Cross-Sectional Studies ,Female ,RC870-923 ,medicine.symptom ,business - Abstract
Introduction: Urinary tract symptoms and infection have been associated with occupational factors that impact hydration habits particularly in women. We compared self-reported urinary symptoms and infection and hydration habits between nurses and other occupations in dialysis units. Methods: Cross-sectional study. Participants worked in five nephrology centers in Brazil and answered an online questionnaire comprising questions regarding urinary tract symptoms and infection episodes in the preceding year; data on usual daily beverage intake, urine frequency, and urine color according to a urine color chart were also collected, as well as perceptions of water access and toilet adequacy at work. Results: We included 133 women (age=36.9±9.5 years). The self-reported usual daily beverage intake was 6.6±2.9 cups/day (~1320 mL), daily urine frequency was 5.4±2.1, and urine color chart score: 3.0±1.2. Nurses (N=66/49.6%) reported higher prevalence of burning sensation (50 versus 27%; P
- Published
- 2021
41. Impact of Pelvic Radiation Therapy for Prostate Cancer on Global Metabolic Profiles and Microbiota-Driven Gastrointestinal Late Side Effects: A Longitudinal Observational Study
- Author
-
Jervoise Andreyev, Matthew R. Lewis, Caroline Sands, Jia V. Li, David P. Dearnaley, Julian Marchesi, Sarah L. Gulliford, Miguel Reis Ferreira, Elena Chekmeneva, Medical Research Council, and National Institute for Health Research (NIHR)
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,0299 Other Physical Sciences ,Urology ,medicine.medical_treatment ,MEDLINE ,Urine ,Butyrate ,Bioinformatics ,Gastroenterology ,TOXICITY ,Pelvis ,Prostate cancer ,MARKERS ,Prostate ,Internal medicine ,Metabolome ,Metabolomics ,Humans ,Medicine ,1112 Oncology and Carcinogenesis ,Radiology, Nuclear Medicine and imaging ,Oncology & Carcinogenesis ,Microbiome ,Clinical Investigation ,Feces ,PREOPERATIVE RADIOTHERAPY ,OUTCOMES ,Science & Technology ,Radiation ,business.industry ,Microbiota ,Radiology, Nuclear Medicine & Medical Imaging ,Prostatic Neoplasms ,1103 Clinical Sciences ,medicine.disease ,Gastrointestinal Microbiome ,Radiation therapy ,medicine.anatomical_structure ,Oncology ,Observational study ,business ,Life Sciences & Biomedicine ,INFLAMMATORY-BOWEL-DISEASE - Abstract
Purpose Radiation therapy to the prostate and pelvic lymph nodes (PLNRT) is part of the curative treatment of high-risk prostate cancer. Yet, the broader influence of radiation therapy on patient physiology is poorly understood. We conducted comprehensive global metabolomic profiling of urine, plasma, and stools sampled from patients undergoing PLNRT for high-risk prostate cancer. Methods and Materials Samples were taken from 32 patients at 6 timepoints: baseline, 2 to 3 and 4 to 5 weeks of PLNRT; and 3, 6, and 12 months after PLNRT. We characterized the global metabolome of urine and plasma using 1H nuclear magnetic resonance spectroscopy and ultraperformance liquid chromatography-mass spectrometry, and of stools with nuclear magnetic resonance. Linear mixed-effects modeling was used to investigate metabolic changes between timepoints for each biofluid and assay and determine metabolites of interest. Results Metabolites in urine, plasma and stools changed significantly after PLNRT initiation. Metabolic profiles did not return to baseline up to 1 year post-PLNRT in any biofluid. Molecules associated with cardiovascular risk were increased in plasma. Pre-PLNRT fecal butyrate levels directly associated with increasing gastrointestinal side effects, as did a sharper fall in those levels during and up to 1 year postradiation therapy, mirroring our previous results with metataxonomics. Conclusions We showed for the first time that an overall metabolic effect is observed in patients undergoing PLNRT up to 1 year posttreatment. These metabolic changes may effect on long-term morbidity after treatment, which warrants further investigation.
- Published
- 2021
42. Identification of urinary peptides associated with allergic rhinitis
- Author
-
Nan Liu, Ming Zhang, and Jianfang Wang
- Subjects
chemistry.chemical_classification ,medicine.medical_specialty ,Diagnostic methods ,Receiver operating characteristic ,business.industry ,Urinary system ,Area under the curve ,Peptide ,Urine ,Proteomics ,Gastroenterology ,chemistry ,Internal medicine ,Potential biomarkers ,medicine ,Immunology and Allergy ,business - Abstract
Background Whether in the world or in China, allergic rhinitis (AR) is a disease that seriously affects people's daily life. However, the current diagnostic methods for AR still have certain limitations. Adopt matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for proteomics detection makes it possible to screen for high-throughput biomarkers. This study intends to use this technology to analyze the urine proteomics of AR patients to find potential biomarkers for AR diagnosis. Materials and methods Urine and clinical data were collected from 60 patients with AR and 60 healthy controls. MALDI-TOF-MS based on weak cation exchange magnetic beads was used to obtain and analyze the urine peptide profiles between AR patients and healthy controls. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic ability of identified peptides and proteins. Results The results revealed significantly different expressions of nine peptide peaks between AR and healthy controls (ROC area under the curve [AUC], 0.660–0.780). We constructed a diagnostic model containing seven proteins identified from the above-mentioned peptide peaks. The AUC of the seven peptides panel for AR diagnosis was 0.882 with 75.0% sensitivity and 90.0% specificity. Conclusions The seven peptides diagnostic model established in this study had high specificity and moderate sensitivity in the diagnosis of AR. MALDI-TOF MS is a potential tool for diagnosing AR by the use of urine samples.
- Published
- 2021
43. Urinary estrogen metabolites and breast cancer risk in Chinese population
- Author
-
Hongjiang Li, Xiaomin Li, Xiaoqin Yang, and Ling Fang
- Subjects
Oncology ,liquid chromatography tandem mass spectrometry ,medicine.medical_specialty ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Metabolite ,Urinary system ,Urine ,Logistic regression ,estrogen metabolism ,Diseases of the endocrine glands. Clinical endocrinology ,chemistry.chemical_compound ,Endocrinology ,Breast cancer ,breast cancer ,Internal medicine ,Internal Medicine ,medicine ,Morning ,Chinese population ,business.industry ,Research ,hormone receptor ,medicine.disease ,RC648-665 ,chemistry ,Estrogen ,business ,estrogen receptor - Abstract
Background In China, the association between estrogen metabolism and breast cancer risk and the differences in metabolic pattern between breast cancer patients and controls are poorly understood. Methods A total of 84 patients with invasive breast cancer and 47 controls with benign breast diseases were included in this study. Estrogen metabolites from their morning urine were determined by HPLC-MS/MS and evaluated in both groups, and the predictive value of each estrogen metabolite in the malignant group according to their menstrual status was analyzed. Results Urinary concentration of estrogen metabolites 2-hydroxyestrone (2-OHE1), 2-hydroxyestradiol (2-OHE2), 4-hydroxyestradiol (4-OHE2), 4-methoxyestrone (4-MeOE1), and 16α-hydroxyestrone were lower in postmenopausal patients with breast cancer, compared with benign controls. In logistic regression model, breast cancer risk increased with the decline in the levels of 4-OHE2 and 4-MeOE1. In premenopausal patients, a difference in the level of 2-OHE2 was observed between both groups, and 2-OHE2 was found to have predictive value for breast cancer. Additionally, urinary 2-OHE2 level in premenopausal hormone receptor positive (HR+) patients was considerably higher compared with hormone receptor negative patients. Conclusions We found that lower urinary levels of 4-OHE2 and 4-MeOE1 had predictive value for breast cancer, and higher 2-OHE1 were associated with HR+ breast cancer in premenopausal women.
- Published
- 2021
44. Association between aortic calcification and the presence of kidney stones: calcium oxalate calculi in focus
- Author
-
Liang Zhou, Kunjie Wang, Yu Liu, Yin Tang, Hong Li, Xi Jin, Bo Li, and Yan Huang
- Subjects
Nephrology ,medicine.medical_specialty ,Urology ,Calcium oxalate ,Urine ,Gastroenterology ,Kidney Calculi ,chemistry.chemical_compound ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,medicine ,Humans ,Calculus (medicine) ,Retrospective Studies ,Calcium Oxalate ,business.industry ,Confounding ,medicine.disease ,chemistry ,Case-Control Studies ,Hypertension ,Uric acid ,Kidney stones ,business - Abstract
Purpose The current research is aimed at analyzing the relationship between kidney stone (KS) and abdominal aortic calcification (AAC) and the relationship between KS components and AAC. Methods This is a retrospective, case–control study. Kidney stone formers (KSFs) were treated at the Department of Urology, West China Hospital, Sichuan University for urological calculus disease from January 2014 to January 2020. Matched non-stone formers (non-SFs) were drawn from the same hospital for routine health examination from January 2018 to February 2019. Research-related information was collected and reviewed retrospectively from the hospital’s computerized records. AAC were evaluated using available results of computed tomography imaging and abdominal vascular ultrasound. The relationships of AAC between KSFs and non-SFs were compared. The composition of renal calculi was analyzed by Fourier-transform infrared spectrophotometer. KSFs were divided into AAC groups and non-AAC based on AAC. The relationship of the composition of renal calculi between AAC and non-AAC were compared. The independent-sample t test, the chi-squared test and binary logistics regression were performed. Results Altogether, 4516 people were included, with 1027 KSFs and 3489 non-SFs. There were no significant differences in the laboratory parameters between KSFs and non-SFs. The association between the presence of AAC and KS was significant in multivariable model 2 [adjusting hypertension, diabetes mellitus, fasting blood glucose, uric acid, serum triglyceride (TG), serum calcium, and urine pH] (OR 5.756, 95% CI 4.616–7.177, p p = 0.048). Conclusions The current study pioneered the revelation of the relationship between CaOx and AAC. Through an elimination of the confounding factors, the study demonstrated that KS and AAC were connected.
- Published
- 2021
45. Bladder cancer biomarker screening based on non-targeted urine metabolomics
- Author
-
Peiming Bai, Chuanchuan Zhan, Hongbing Xie, Shipeng Li, Jinkun Li, and Bisheng Cheng
- Subjects
Nephrology ,medicine.medical_specialty ,Bladder cancer ,medicine.diagnostic_test ,business.industry ,Urology ,Urinary system ,Metabolite ,Urine ,Cystoscopy ,medicine.disease ,chemistry.chemical_compound ,Metabolomics ,Urinary Bladder Neoplasms ,chemistry ,Internal medicine ,Biomarkers, Tumor ,Metabolome ,Humans ,Medicine ,Biomarker (medicine) ,business ,Early Detection of Cancer - Abstract
Purpose Bladder cancer is one of the most common malignancies of the urinary system, and its screening relies heavily on invasive cystoscopy, which increases the risk of urethral injury and infection. This study aims to use non-targeted metabolomics methods to screen for metabolites that are significantly different between the urine of bladder cancer patients and cancer-free controls. Methods In this study, liquid chromatography-mass spectrometry was used to analyze the urine of bladder cancer patients (n = 57) and the cancer-free controls (n = 38) by non-targeted metabolomic analysis and metabolite identification. Results The results showed that there were significant differences in the expression of 27 metabolites between bladder cancer patients and the cancer-free controls. Conclusion In the multivariate statistical analysis of this study, the urinary metabolic profile data of bladder cancer patients were analyzed, and the receiver operating characteristic curve analysis showed that it is possible to perform non-invasive clinical diagnoses of bladder cancer through these candidate biomarkers.
- Published
- 2021
46. The impact of stenting prior to oral chemolysis of upper urinary tract uric acid stones
- Author
-
Arman Tsaturyan, Kevin Stritt, Piet Bosshard, Beat Roth, Elizaveta Bokova, and Olivier Bonny
- Subjects
Nephrology ,medicine.medical_specialty ,Sodium bicarbonate ,business.industry ,Urology ,Urinary system ,medicine.medical_treatment ,Stent ,Urine ,Uric Acid Urolithiasis ,chemistry.chemical_compound ,chemistry ,Internal medicine ,Uric acid ,Medicine ,business ,Upper urinary tract - Abstract
PURPOSE To evaluate the impact of ureteral stenting on the success rate of oral chemolysis in the management of suspected uric acid upper urinary tract (UUT) stones. METHODS Retrospective matched-pair analysis of 172 patients treated with oral chemolysis from 01/2010 to 12/2019. Patients with low density (upon non-contrast enhanced computer tomography [NCCT]), radiolucent (on plain radiography) urinary stones, a low urine pH (
- Published
- 2021
47. Hypertension and diabetes mellitus: a collision of two heavyweight non-communicable diseases
- Author
-
Pillay Somasundram
- Subjects
Creatinine ,medicine.medical_specialty ,Proteinuria ,Waist ,business.industry ,Endocrinology, Diabetes and Metabolism ,Type 2 Diabetes Mellitus ,Urine ,medicine.disease ,Obesity ,chemistry.chemical_compound ,Endocrinology ,Blood pressure ,chemistry ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,BMI, COVID-19 infection, diabetes mellitus, glycaemic control, hypertension, lipid control, macrovascular complications, microvascular complications, obesity, proteinuria, resistant hypertension, waist circumference ,medicine.symptom ,business - Abstract
Introduction: Patients living with type 2 diabetes mellitus (PLWD) are at an increased risk of developing hypertension (HPT). The presence of HPT in PLWD (PLWDH) accelerates diabetes-related complications (DRC). Scarce data exist from South Africa on the impact of HPT in PLWD.Methods: Data werecaptured from Edendale Hospital diabetes clinic datasheets from January 1, 2019 to December 31, 2019 and analysed to determine differences in demographic, clinical and biochemical variables between PLWD and PLWDH.Results: Data from 822 PLWD were analysed, the majority having HPT (713,86.74%). The prevalence of HPT, resistant HPT (RHPT) and the number of antihypertensives used increased with age and diabetes duration. PLWDH had statistically poorer lipid control (LC), higher creatinine, waist circumference (WC), increased prevalence of sensory peripheral neuropathy, non-proliferative and proliferative retinopathy, cerebrovascular accidents, proteinuria and renal impairment. The significant majority of PLWDH were not meeting diabetes targets (glycaemic, lipid, BMI, WC). The bulk of PLWDH were on combination antihypertensive therapy (p < 0.001) and performed significantly better than monotherapy for glycaemia, LC, BMI and WC. Proteinuria and blood pressure (BP) improved significantly as the number of antihypertensives increased. One-fifth (151, 18.37%) of PLWDH had RHPT; this was more common in females (p < 0.001). PLWD with RHPT had a significantly higher LDL cholesterol, BMI, and urine protein–creatinine ratio (p < 0.001). Over one-quarter (29.87%) of the PLWD without HPT had a BP over 140/90mmHg.Conclusion: It was shown that HPT, RHPT and obesity are significant comorbidities in PLWD and increase the risk of DRC. The majority of PLWDH are not meeting targets, which places them at increased risk of DRC. BP, glycaemic and LC and proteinuria improved in those on combination antihypertensive therapy. A significant proportion of PLWD without HPT had elevated BP, and thus were potentially undiagnosed hypertensives needing intervention.
- Published
- 2021
48. Efficacy of Restricting Dietary Protein Intake Combined with Buyang Huanwu Decoction in Treating Diabetic Nephropathy and Its Effect on Patients’ Inflammatory Factor Levels
- Author
-
Dan Chen, Dan Huang, Tao-Tao Hu, and Fang Chen
- Subjects
medicine.medical_specialty ,Creatinine ,Article Subject ,business.industry ,education ,Renal function ,Urine ,Traditional Chinese medicine ,medicine.disease ,behavioral disciplines and activities ,Gastroenterology ,Diabetic nephropathy ,Other systems of medicine ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Buyang huanwu decoction ,Internal medicine ,medicine ,Microalbuminuria ,business ,RZ201-999 ,psychological phenomena and processes ,Dietary protein intake ,Research Article - Abstract
Objective. To study the efficacy of restricting dietary protein intake combined with Buyang Huanwu decoction in treating diabetic nephropathy (DN) and its effect on patients’ inflammatory factor levels. Methods. The medical data of 150 DN patients treated in Wuhan No.1 Hospital (June 2018—May 2021) were retrospectively analyzed. All patients received regular therapy, those who received the intervention of restricting dietary protein intake were included in the control group (n = 75), and on this basis, those treated with Buyang Huanwu decoction were included in the experimental group (n = 75), so as to scientifically evaluate their efficacy and inflammatory factor levels after treatment. Results. The patients’ general information was not statistically different between the two groups ( P > 0.05 ); after treatment, the experimental group gained remarkably higher marked effective rate and total effective rate of treatment than the control group ( P < 0.05 ); the inflammatory factor levels of all patients were obviously better than before ( P < 0.05 ), and the levels of TNF-α, IL-2, IL-8, IL-4, and IL-10 were obviously lower in the experimental group than in the control group ( P < 0.05 ); the levels of fasting blood glucose, 2 h postprandial blood glucose, and glycosylated hemoglobin of all patients were remarkably lower than before ( P < 0.05 ), but with no significant between-group difference ( P > 0.05 ); the renal function indexes of all patients were better than before, and between the two groups, the levels of 24 h microalbuminuria, 24 h urine protein excretion, and serum creatinine were obviously lower and the glomerular filtration rate was significantly higher in the experimental group ( P all P < 0.05 ). Conclusion. Jointly applying Buyang Huanwu decoction on the basis of restricting dietary protein intake can effectively promote the clinical efficacy of DN, which is conducive to adjusting the inflammatory factor levels, promoting the patients’ renal function, and alleviating the clinical symptoms.
- Published
- 2021
49. Therapeutic failures of targeted antibiotic prophylaxis in urology
- Author
-
Pascal Blanchet, Yvanne Sadreux, Samuel Markowicz, Sunniva Donat, Laurent Brureau, Pierre-Marie Roger, and Elodie Curlier
- Subjects
Male ,Microbiology (medical) ,medicine.medical_specialty ,Bacteriuria ,Urology department ,medicine.drug_class ,Urology ,Antibiotics ,Urine ,Urinary Catheters ,Medical microbiology ,Internal medicine ,Trimethoprim, Sulfamethoxazole Drug Combination ,medicine ,Enterococcus spp ,Humans ,Prospective Studies ,Treatment Failure ,Antibiotic prophylaxis ,Aged ,Aged, 80 and over ,Bacteria ,business.industry ,Amoxicillin ,General Medicine ,Antibiotic Prophylaxis ,Middle Aged ,Urological surgery ,Anti-Bacterial Agents ,Infectious Diseases ,Urinary Tract Infections ,Female ,Stents ,business ,medicine.drug - Abstract
Targeted antibiotic prophylaxis (TAP) is required for patients with positive urine culture before urological surgery. Our aim was to determine the efficacy of TAP. This was a prospective single-center study performed in a urology department. All patients who underwent a programmed surgery were included. Urine culture was obtained before surgery requiring a prophylaxis: in the case of sterile urines, antibiotics were used in accordance with national recommendations; for positive urine culture, a TAP was used in accordance with susceptibility testing. The drugs were administered for 2 days before surgery until withdrawal of bladder catheter. The occurrence of healthcare-associated infections was registered until day 30 after surgery. Two hundred three patients were included for 8 non-consecutive weeks in 2020, among whom fifteen were lost of sight before day 30. Among the remaining 188 patients, most frequent surgeries were 75 prostatic diseases (40%), 50 endo-ureteral surgeries for JJ stent insertion (27%), and 23 bladder cancers (12%). One hundred forty-eight (79%) patients required a urine culture before procedure; 142/148 (96%) urine cultures were performed, leading to 74 TAP. The main isolated bacteria were 48 Enterobacteriaceae and 8 Enterococcus spp. TAP was cotrimoxazole (n = 30), aminoglycosides (n = 11), amoxicillin (n = 9), fluoroquinolones (n = 7), and others (n = 17). The rate of healthcare-associated infections was 14.8% (11/74), including six microbiologically documented antibiotic failures. The rate of healthcare-associated infection after urological surgery using TAP was high, implying to discuss the choice and the dosage of the antibiotic molecules.
- Published
- 2021
50. Racial Differences in Urine Testing of Febrile Young Children Presenting to Pediatric Hospitals
- Author
-
Nader Shaikh, Sriram Ramgopal, Timothy R Shope, Nichell Tidwell, and Michelle L. Macy
- Subjects
medicine.medical_specialty ,Health (social science) ,Sociology and Political Science ,Urinalysis ,medicine.diagnostic_test ,business.industry ,Health Policy ,Urinary system ,Public Health, Environmental and Occupational Health ,Retrospective cohort study ,Urine ,Emergency department ,Lower risk ,Confidence interval ,Anthropology ,Internal medicine ,Epidemiology ,Medicine ,business - Abstract
Dating back to 2011, practice guidelines considered Black race a factor associated with lower risk of urinary tract infection (UTI). Race-based clinical decisions raise concerns about potential treatment disparities. We investigate urine testing (urinalysis and/or urine culture) among young febrile children in the emergency department (ED), revisits, and potential missed diagnoses by race/ethnicity. We performed a multicenter retrospective cohort study of children 2–24 months evaluated in 26 US EDs from 2009 to 2019 with a fever diagnosis. We evaluated longitudinal testing trends, constructed a generalized linear mixed-effects model to identify the association of race/ethnicity with testing, and characterized UTI diagnoses and ≤ 7-day revisits. Of 734,730 included patients, 24.1% were Black. Variation in urine testing was observed by patient race/ethnicity (23.4% Black, 31.7% White, 33.9% Hispanic, 30.0% other race). Relative differences in testing persisted over time. Black patients had lower adjusted odds of testing (0.70, 95% confidence interval [CI] 0.69–0.71). Among patients with urine testing, 2.4% (95% CI 2.3–2.6%) of Black and 3.3% (95% CI 3.1–3.4%) of White patients were diagnosed with UTI. Among Black patients with urine testing on the index visit, 8.5% (95% CI 8.2–8.8%) had return visits compared to 7.6% (95% CI 7.5–7.8%) among those without urine testing on index visit. Among patients with urine testing on revisit, UTI diagnosis was similar by race/ethnicity. Black patients had lower rates of urine testing and UTI diagnoses relative to other racial/ethnic groups. This was not associated with higher rates of missed diagnoses or unscheduled return visits.
- Published
- 2021
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.