9 results on '"Takahiro Kawase"'
Search Results
2. Heat-killed Lactobacillus helveticus strain MCC1848 confers resilience to anxiety or depression-like symptoms caused by subchronic social defeat stress in mice
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Atsushi Toyoda, Jin-zhong Xiao, Tetsuya Kuhara, Yodai Kobayashi, Eri Mitsuyama, Takamitsu Tsukahara, Takahiro Kawase, and Hazuki Maehata
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0301 basic medicine ,Nervous system ,medicine.medical_specialty ,Gut–brain axis ,Central nervous system ,Gut flora ,Nucleus accumbens ,Applied Microbiology and Biotechnology ,Biochemistry ,Analytical Chemistry ,Social defeat ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Molecular Biology ,Lactobacillus helveticus ,biology ,Organic Chemistry ,General Medicine ,biology.organism_classification ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Anxiety ,medicine.symptom ,030217 neurology & neurosurgery ,Biotechnology - Abstract
The gut microbiota is involved in the pathogenesis of stress-related disorders. Probiotics can benefit the central nervous system via the microbiota–gut–brain axis, which raises the possibility that probiotics are effective in managing depression. In the present study, we examined the effects of heat-killed Lactobacillus helveticus strain MCC1848 in subchronic and mild social defeat stress (sCSDS) model mice (a widely used animal model of depression). MCC1848 supplementation significantly enhanced the interaction time in the social interaction test and sucrose preference ratio in the sucrose preference test, suggesting that MCC1848 improved anxiety- or depressive-like behaviors in sCSDS mice. The gene expression profile analysis of the nucleus accumbens, which plays an important role in stress resilience, indicated that MCC1848 ameliorated sCSDS-induced gene expression alterations in signal transduction or nervous system development. These findings suggest that MCC1848 supplementation is useful as a preventive strategy for chronic-stress-induced depression. Overview of anti-anxiety or depressant-like effects of heat-killed L. helevticus MCC1848.
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- 2019
3. Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations
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Kuniyasu Ochiai, Takamitsu Tsukahara, Atsushi Toyoda, Shin-ichi Nakamura, and Takahiro Kawase
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0301 basic medicine ,Lipopolysaccharides ,Male ,medicine.medical_specialty ,Lipopolysaccharide ,medicine.medical_treatment ,Immunology ,Gingiva ,Hippocampus ,lcsh:RC346-429 ,Mouse model ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Neurotrophic factors ,Internal medicine ,Blood plasma ,medicine ,Animals ,Porphyromonas gingivalis ,lcsh:Neurology. Diseases of the nervous system ,biology ,Behavior, Animal ,General Neuroscience ,Research ,Interleukin ,biology.organism_classification ,Fatty Acids, Volatile ,030104 developmental biology ,Cytokine ,Endocrinology ,Neurology ,chemistry ,Gingival Diseases ,Abnormal behavior ,Butyric Acid ,Cytokines ,Tumor necrosis factor alpha ,n-Butyric acid ,030217 neurology & neurosurgery ,Acetylcholine ,medicine.drug - Abstract
Background Periodontopathic bacteria such as Porphyromonas gingivalis produce several metabolites, including lipopolysaccharide (LPS) and n-butyric acid (BA). Past work suggested that periodontal infection may cause cognitive impairment in mice. Aims To elucidate the mechanisms by which metabolites such as LPS and BA, resulting from Porphyromonas gingivalis activity, induce immunological and physiological abnormalities in mice. Methods In the present work, 28 male ICR mice were placed in an open-field arena and the total distance (cm/600 s) they covered was recorded. Based on their moving distances, mice were divided into 4 groups (n = 7) and injected the following substances into their gingival tissues for 32 consecutive days: saline (C), 5 mmol/L of BA (B), 1 μg/mouse of LPS (L), and BA-LPS (BL) solutions. Distances covered by mice were also measured on days 14 and 21, with their habituation scores considered as “(moving distance on day 14 or 21)/(moving distance on day 0)”. Afterwards, mice were dissected, and hippocampal gene expression and the concentrations of short-chain fatty acids, neurotransmitters and cytokines in their blood plasma and brains were analyzed. In addition, mouse brain and liver tissues were fixed and visually assessed for histopathological abnormalities. Results Group BL had significantly higher habituation scores than C and B on day 14. LPS induced higher habituation scores on day 21. LPS induced significant decreases in the mRNA levels of interleukin (IL)-6 and brain-derived neurotrophic factors, and an increase in neurotrophic tyrosine kinase receptor type 2. In both plasma and brain, LPS induced a significant acetate increase. Moreover, LPS significantly increased acetylcholine in brain. In plasma alone, LPS and BA significantly decreased monocyte chemoattractant protein 1 (MCP-1). However, while LPS significantly decreased tyrosine, BA significantly increased it. Lastly, LPS significantly decreased IL-6 and tumor necrosis factor in plasma. No histopathological abnormalities were detected in liver or brain tissues of mice. Conclusion We showed that injections of LPS and/or BA induced mice to move seemingly tireless and that both LPS and BA injections strongly induced a reduction of MCP-1 in blood plasma. We concluded that LPS and BA may have been crucial to induce and/or aggravate abnormal behavior in mice.
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- 2020
4. Effects of dietary intake of heat-inactivated Lactobacillus gasseri CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model
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Atsushi Toyoda, Takahiro Kawase, and Takamitsu Tsukahara
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0301 basic medicine ,Male ,medicine.medical_specialty ,Hot Temperature ,Central nervous system ,Hippocampus ,Lactobacillus gasseri ,General Biochemistry, Genetics and Molecular Biology ,Social defeat ,03 medical and health sciences ,Eating ,Mice ,0302 clinical medicine ,Neurotrophic factors ,Internal medicine ,medicine ,Animals ,Mice, Inbred ICR ,biology ,Behavior, Animal ,business.industry ,General Medicine ,biology.organism_classification ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Nerve growth factor ,Mood disorders ,Gene Expression Regulation ,biology.protein ,business ,030217 neurology & neurosurgery ,Stress, Psychological ,Neurotrophin - Abstract
The intestinal ecosystem is involved in the pathogenesis of mood disorders such as depression. Intestinal microbes can affect the central nervous system through the gut-brain axis, which raises the possibility of using probiotics for preventing depression. In this study, we examined the effect of heat-inactivated Lactobacillus gasseri CP2305 (CP2305) in a subchronic and mild social defeat stress (sCSDS) mouse model. sCSDS suppressed food intake. However, dietary CP2305 intake rescued it, suggesting that CP2305 improved the decreased appetite in sCSDS mice. sCSDS did not alter the gene expression of brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 in the hippocampus. However, dietary CP2305 provided following sCSDS increased the gene expression of these neurotrophins in the hippocampus. These findings suggest that CP2305 supplementation would aid in preventing psychosocial stress-induced disorders.
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- 2020
5. Hippocampal metabolism of amino acids by L-amino acid oxidase is involved in fear learning and memory
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Takahiro Kawase, Kentaro Nagaoka, Shozo Tomonaga, Gen Watanabe, Haolin Zhang, Wanzhu Jin, Yuko Shigeno, Takamitsu Tsukahara, Kazuki Fujii, Susumu Fukuzawa, and Kento Usuda
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Male ,0301 basic medicine ,medicine.medical_specialty ,Pyruvate Kinase ,lcsh:Medicine ,Phenylalanine ,Hippocampal formation ,L-Amino Acid Oxidase ,Hippocampus ,Gene Expression Regulation, Enzymologic ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Memory ,Internal medicine ,medicine ,Animals ,Glycolysis ,RNA, Messenger ,Amino Acids ,lcsh:Science ,Mice, Knockout ,chemistry.chemical_classification ,Neurotransmitter Agents ,Multidisciplinary ,Chemistry ,lcsh:R ,Phenylpyruvic acid ,Fear ,Metabolism ,Amino acid ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,Metabolome ,lcsh:Q ,030217 neurology & neurosurgery ,Acetylcholine ,Pyruvate kinase ,medicine.drug - Abstract
Amino acids participate directly and indirectly in many important biochemical functions in the brain. We focused on one amino acid metabolic enzyme, L-amino acid oxidase (LAO), and investigated the importance of LAO in brain function using LAO1 knockout (KO) mice. Compared to wild-type mice, LAO1 KO mice exhibited impaired fear learning and memory function in a passive avoidance test. This impairment in LAO1 KO mice coincided with significantly reduced hippocampal acetylcholine levels compared to wild-type mice, while treatment with donepezil, a reversible acetylcholine esterase inhibitor, inhibited this reduction. Metabolomic analysis revealed that knocking out LAO1 affected amino acid metabolism (mainly of phenylalanine [Phe]) in the hippocampus. Specifically, Phe levels were elevated in LAO1 KO mice, while phenylpyruvic acid (metabolite of Phe produced largely by LAO) levels were reduced. Moreover, knocking out LAO1 decreased hippocampal mRNA levels of pyruvate kinase, the enzymatic activity of which is known to be inhibited by Phe. Based on our findings, we propose that LAO1 KO mice exhibited impaired fear learning and memory owing to low hippocampal acetylcholine levels. Furthermore, we speculate that hippocampal Phe metabolism is an important physiological mechanism related to glycolysis and may underlie cognitive impairments, including those observed in Alzheimer’s disease.
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- 2018
6. Long-term consumption of dried bonitodashi(a traditional Japanese fish stock) reduces anxiety and modifies central amino acid levels in rats
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Mitsuhiro Furuse, Takahiro Kawase, Hiromi Ikeda, Takashi Kondoh, D. Michael Denbow, Shoichiro Funatsu, and Mao Nagasawa
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Male ,Ornithine ,medicine.medical_specialty ,Phenylalanine ,Medicine (miscellaneous) ,Anxiety ,Hippocampus ,Open field ,chemistry.chemical_compound ,Cystathionine ,Methionine ,Oral administration ,Valine ,Cerebellum ,Internal medicine ,medicine ,Animals ,Ingestion ,Anserine ,Palatability ,Amino Acids ,Rats, Wistar ,Bonito ,Nutrition and Dietetics ,Behavior, Animal ,Depression ,business.industry ,General Neuroscience ,Fishes ,General Medicine ,Diet ,Rats ,Hydroxyproline ,Endocrinology ,Seafood ,chemistry ,Asparagine ,business ,2-Aminoadipic Acid - Abstract
Dried bonito dashi, a traditional Japanese fish stock, enhances palatability of various dishes because of its specific flavor. Daily intake of dashi has also been shown to improve mood status such as tension-anxiety in humans. This study aimed at investigating beneficial effects of dashi ingestion on anxiety/depression-like behaviors and changes in amino acid levels in the brain and plasma in rats. Male Wistar rats were given either dried bonito dashi or water for long-term (29 days; Experiment 1) or single oral administration (Experiment 2). Anxiety and depression-like behaviors were tested using the open field and forced swimming tests, respectively. Concentrations of amino acids were measured in the hippocampus, hypothalamus, cerebellum, and jugular vein. During the long-term (29 days) consumption, rats given 2% dashi frequently entered the center zone and spent more time compared with the water controls in the open field test. However, the dashi was ineffective on depression-like behavior. In the hippocampus, concentrations of hydroxyproline, anserine, and valine were increased by dashi while those of asparagine and phenylalanine were decreased. In the hypothalamus, the methionine concentration was decreased. In a single oral administration experiment, the dashi (1%, 2% or 10%) showed no effects on behaviors. Significance was observed only in the concentrations of α-aminoadipic acid, cystathionine, and ornithine in the hippocampus. Dried bonito dashi is a functional food having anxiolytic-like effects. Daily ingestion of the dashi, even at lower concentrations found in the cuisine, reduces anxiety and alters amino acid levels in the brain.
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- 2014
7. Photoperiodic responses of depression-like behavior, the brain serotonergic system, and peripheral metabolism in laboratory mice
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Mitsuhiro Furuse, Ayaka Iwamoto, Takahiro Kawase, Yuki Togo, Tsuyoshi Otsuka, Ryosei Goda, Mao Nagasawa, Misato Kawai, Haruka Matsuo, and Shinobu Yasuo
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Male ,Serotonin ,medicine.medical_specialty ,Photoperiod ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Biology ,Serotonergic ,Amygdala ,Mice ,Endocrinology ,Internal medicine ,medicine ,Animals ,Biological Psychiatry ,Depressive Disorder ,Mice, Inbred ICR ,Raphe ,Endocrine and Autonomic Systems ,Insulin ,Brain ,Seasonal Affective Disorder ,Metabolism ,Peripheral ,Mice, Inbred C57BL ,Psychiatry and Mental health ,Glucose ,medicine.anatomical_structure ,Models, Animal ,Mice, Inbred CBA ,Energy Metabolism ,Signal Transduction ,Behavioural despair test - Abstract
Seasonal affective disorder (SAD) is characterized by depression during specific seasons, generally winter. The pathophysiological mechanisms underlying SAD remain elusive due to a limited number of animal models with high availability and validity. Here we show that laboratory C57BL/6J mice display photoperiodic changes in depression-like behavior and brain serotonin content. C57BL/6J mice maintained under short-day conditions, as compared to those under long-day conditions, demonstrated prolonged immobility times in the forced swimming test with lower brain levels of serotonin and its precursor l-tryptophan. Furthermore, photoperiod altered multiple parameters reflective of peripheral metabolism, including the ratio of plasma l-tryptophan to the sum of other large neutral amino acids that compete for transport across the blood-brain barrier, responses of circulating glucose and insulin to glucose load, sucrose intake under restricted feeding condition, and sensitivity of the brain serotonergic system to peripherally administered glucose. These data suggest that the mechanisms underlying SAD involve the brain-peripheral tissue network, and C57BL/6J mice can serve as a powerful tool for investigating the link between seasons and mood.
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- 2014
8. Suppressed expression of cystathionine β-synthase and smaller cerebellum in Wistar Kyoto rats
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Takahiro Kawase, Mao Nagasawa, Ayaka Iwamoto, Hiromi Ikeda, Shinobu Yasuo, and Mitsuiro Furuse
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medicine.medical_specialty ,Cerebellum ,Metabolite ,Racemases and Epimerases ,Cystathionine beta-Synthase ,Rats, Inbred WKY ,Gene Expression Regulation, Enzymologic ,Serine ,chemistry.chemical_compound ,Internal medicine ,medicine ,Hippocampus (mythology) ,Animals ,RNA, Messenger ,Rats, Wistar ,Molecular Biology ,Analysis of Variance ,ATP synthase ,biology ,Depression ,General Neuroscience ,Body Weight ,Age Factors ,Metabolism ,Organ Size ,Cystathionine beta synthase ,Rats ,Disease Models, Animal ,Endocrinology ,medicine.anatomical_structure ,nervous system ,chemistry ,Biochemistry ,Serine racemase ,biology.protein ,Neurology (clinical) ,Developmental Biology - Abstract
We previously reported that Wistar Kyoto rats, an animal model of depression, have a characteristically abnormal serine metabolism in the brain, i.e., lower serine and cystathionine, which is a metabolite of serine, concentrations in the brain. To explore the mechanism underlying this abnormality, the expression of cystathionine β-synthase and serine racemase, which are the enzymes involved in the serine metabolism, was investigated in the cerebellum and hippocampus of Wistar and Wistar Kyoto rats. Wistar Kyoto rats exhibited a significantly lower mRNA expression of cystathionine β-synthase in the cerebellum in comparison with Wistar rats, while expression levels in the hippocampus did not differ between strains. Previous study indicated that the reduction of cystathionine β-synthase in the brain induced cerebellar aplasia in mice. Therefore, the cerebellar size was compared between Wistar rats and Wistar Kyoto rats. Wistar Kyoto rats displayed a lower ratio of cerebellum weight to whole-brain weight compared with Wistar rats of the same generation or similar body weight, suggesting that Wistar Kyoto rats exhibit smaller cerebellum. These results suggest that the lower mRNA expression of cystathionine β-synthase in the cerebellum and the smaller size of cerebellum may be related to the depression-like behavior in Wistar Kyoto rats.
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- 2015
9. Metabolism of amino acids differs in the brains of Djungarian hamster (P. sungorus) and Roborovskii hamster (P. roborovskii)
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Hiromi Ikeda, Vishwajit S. Chowdhury, Mao Nagasawa, Shinobu Yasuo, Takahiro Kawase, and Mitsuhiro Furuse
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chemistry.chemical_classification ,medicine.medical_specialty ,Multidisciplinary ,biology ,Research ,L-Tyrosine ,Significant difference ,Brain ,Hamster ,Metabolism ,biology.organism_classification ,Djungarian hamsters ,Roborovskii hamsters ,Locomotor activity ,Amino acid ,Phodopus ,Endocrinology ,Djungarian Hamsters ,chemistry ,Dopamine ,D-Tyrosine ,Internal medicine ,medicine ,medicine.drug - Abstract
Djungarian hamster (P. sungorus) and Roborovskii hamster (P. roborovskii) belong to the same genus of phodopus. Roborovskii hamster shows high locomotor activity and low level of dopamine (DA) in the brain. Administration of L-tyrosine, a precursor of DA, decreases locomotor activity in Roborovskii hamsters. However, the amino acid metabolism in relation to the hyperactivity is not yet well known. In the present study, L- and D-amino acid concentrations in the brain, liver, and plasma in Djungarian and Roborovskii hamsters were investigated during day and night times to explain the possible difference in hyperactivity between them. Most of the examined amino acids were higher in the night time when hamsters are active compared to those in day time. L- and D-tyrosine concentrations were higher in the liver of Roborovskii hamsters than in Djungarian hamsters. Furthermore, brain concentration of D-tyrosine was higher in the Roborovskii than in Djungarian hamsters, but no significant difference was observed for L-tyrosine concentrations between the two species. These results suggest that the conversion of L-tyrosine to D-tyrosine in the brain of Roborovskii hamster may be higher than in Djungarian hamster, which may cause low DA concentration and hyperactivity in Roborovskii hamster. On the other hand, L- and D-serine, which are known as sedative factors, were lower in Roborovskii hamsters than Djungarian hamster. These results suggest that species-specific regulation in amino acid metabolism may contribute to hyperactivity in Roborovskii hamsters.
- Published
- 2014
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