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The effects of dietary saturated and polyunsaturated fat on the metabolism of apolipoprotein A-I (apoA-I) and apolipoprotein B (apoB) were studied in a patient with type IIb hyperlipoproteinaemia. On the saturated fat diet, the rate of synthesis of very low density lipoprotein apoprotein B (VLDL-apoB) was approximately twice normal, accounting for the increased plasma VLDL pool in this subject. However, 54% of the synthesized VLDL-apoB was catabolized by a pathway independent of low density lipoproteins (LDL). The metabolic conversion rate of VLDL-apoB to LDL-apoB was normal in this subject and his expanded plasma LDL-apoB pool resulted, not from increased input of the apoprotein from VLDL, but from a decrease in its fractional clearance rate. On the polyunsaturated diet, there was a significant fall in the plasma cholesterol and triglyceride concentrations and a change in the fatty acid composition of all plasma lipoprotein fractions. These changes were accompanied by a decrease in the plasma concentrations of apoA-I and apoB which resulted from a reduction of apoprotein synthetic rate.

The activities of jejunal carbohydrate-metabolizing enzymes show adaptive changes in response to dietary substances, drugs, and sex hormones. To learn whether insulin, tolbutamide, and glucagon had effects on these enzymes, we performed serial peroral jejunal biopsies in normal young men and in obese patients, before and after treatment with these agents. Jejunal mucosa was assayed for glycolytic enzyme activities, pyruvate kinase (PK), hexokinase (HK), and fructose-1,6-diphosphate aldolase (FDPA), and the nonglycolytic enzyme activity, fructose diphosphatase (FDPase). Insulin significantly increased the activity of jejunal PK (+48% change from control) and HK (+6%), decreased the activity of FDPase (−36%), and had no effect on FDPA. Glucagon had opposite effects; the activity of PK was decreased (−33%) and FDPase was increased (+50%). Tolbutamide significantly increased the activities of PK (+47%), HK (+14%), and FDPA (+7%), and decreased the activities of FDPase (−36%). The results suggest that at least some of the effects of tolbutamide on glycolytic enzyme activities were independent of endogenous insulin. The data support the concept that jejunal carbohydrate-metabolizing enzymes in man respond to hormones and drugs similar to responses observed in rat liver. This is important because it now gives us a means of studying the actions of these hormones directly in human tissue.

Insulin and glucagon injected into the portal vein produced rapid reciprocal changes in the activities of certain hepatic glycolytic enzymes and fructose diphosphatase in the rat. Insulin produced a rapid increase in hepatic phosphofructokinase and pyruvate kinase activities and a decrease in fructose diphosphatase activity. Glucagon produced a rapid but reciprocal response in each of these enzyme activities. Fructose diphosphate aldolase activity was unaltered by either hormone. The insulin effect on these enzymes was detected within 5 min following injection, was maximal by 10 min, and then gradually declined over the remaining 30 min of testing. The magnitude of the insulin effect was dependent on the amount of insulin injected. No effect was detected when 0.005 unit per kg (0.001 unit) was injected; phosphofructokinase and pyruvate kinase activities were slightly increased with 0.015 unit per kg (0.003 unit); phosphofructokinase, pyruvate kinase, and fructose diphosphatase activities were altered with 0.15 unit per kg (0.03 unit) and 1.5 units per kg (0.3 unit), with the higher dose giving a slightly greater effect. The insulin effect was not associated with a significant change in cyclic adenosine 3':5'-monophosphate concentrations. The glucagon effect on the enzyme activities was preceded by a significant increase in cyclic adenosine 3':5'-monophosphate concentrations, which occurred within 30 s after hormone injection. A change in enzyme activities was found at 2 to 5 min and was maximal 5 to 10 min following glucagon injection. The hormone effect on enzyme activities and cyclic adenosine 3':5'-monophosphate levels was dependent on the amount of glucagon injected, being maximal at doses of 150 to 300 µg. Pretreatment of the rat with actinomycin D or puromycin did not alter the response of the enzymes to insulin and glucagon, indicating that de novo protein synthesis was not responsible for the change in enzyme activities. Glucagon injected 5 min following insulin reversed the insulin effect; insulin given 5 min after glucagon also partially reversed the glucagon effect on cyclic adenosine 3':5'-monophosphate and the enzyme activities. Intravenous insulin produced rapid changes in the activities of renal cortical, skeletal muscle, and epididymal fat glycolytic enzymes and fructose diphosphatase in the rat. Insulin (0.15 unit per kg) produced rapid increases in pyruvate kinase and phosphofructokinase and rapid decrease in fructose diphosphatase activities in all tissues. Fructose diphosphate aldolase activity was unchanged following insulin infusion. Intravenous glucagon (0.15 mg) produced rapid changes, reciprocal to those seen with insulin, in fructose diphosphatase activity in all tissues. Glucagon significantly decreased epididymal fat phosphofructokinase activities but did not alter the activity of this enzyme in the renal cortex and skeletal muscle. Glucagon significantly decreased renal cortical pyruvate kinase but had no effect in the epididymal fat and skeletal muscle. Fructose diphosphate aldolase activity was unchanged in all tissues following glucagon infusion. Cyclic adenosine 3':5'-monophosphate infusion (0.05 mmole) produced significant changes in pyruvate kinase, phosphofructokinase, and fructose diphosphatase activities in all tissues which resembled those changes seen following glucagon infusion. These data suggest that the glucagon responses seen in the four tissues studied are mediated by cyclic adenosine 3':5'-monophosphate.

Epinephrine infusion into the portal vein produced rapid changes in the activities of certain hepatic, epididymal fat, and skeletal muscle glycolytic enzymes and fructose diphosphatase in the rat. Epinephrine produced a rapid increase in hepatic, epididymal fat, and skeletal muscle fructose diphosphatase activity. In contrast, epinephrine produced a rapid decrease in hepatic phosphofructokinase, pyruvate kinase, and glucokinase activities. Epinephrine also lowered skeletal muscle phosphofructokinase and pyruvate kinase activities. However, epinephrine had no significant effect upon epididymal fat pyruvate kinase and phosphofructokinase activities. Fructose diphosphate aldolase activities were unchanged in the liver, epididymal fat, and skeletal muscle. The epinephrine effect on the hepatic enzymes was maximal within 2 to 5 min following injection of 2 µg and then gradually returned to control levels over the remaining 35 to 38 min of testing. The magnitude of the epinephrine effect was dose-dependent. No effect was detected when 0.25 µg per min was injected. Phosphofructokinase, pyruvate kinase, and fructose diphosphatase activities were significantly altered at a dose level of 1.0 µg per min, with maximal changes occurring at the 2.0- to 4.0-µg per min dose level. The epinephrine effect was associated with a significant increase in hepatic cyclic adenosine 3′:5′-monophosphate concentrations. Actinomycin D and puromycin treatment did not alter the response of the enzymes to epinephrine. Insulin given 5 min following epinephrine partially reversed the epinephrine effect. Similarly, epinephrine given 5 min following insulin partially reversed the insulin effect. We suggest that the epinephrine effect is mediated through cyclic adenosine 3′:5′-monophosphate and may be due to phosphorylating mechanisms analogous to that responsible for the regulation of glycogen metabolism.

The relative toxicity and metabolic effectiveness of cholecalciferol (CC) and 25-hydroxycholecalciferol (25-HCC) in chicks were evaluated by feeding six graded levels of each and observing gross and microscopic pathology as well as several metabolic parameters of calcium metabolism. Renal tubular calcification was observed when CC was fed at the rate of 10.0 mg/kg of diet and when 25-HCC was fed at the rate of 0.1 mg/kg diet. Thus, 100-fold increase in toxicity results when the hydroxylated form of CC is fed. Both microscopic renal lesions and increased renal calcium and inorganic phosphate concentrations occurred in chicks with normal serum calcium concentrations.

Rate zonal ultracentrifugation of plasma samples from ten healthy age-matched volunteers (five males, five females) indicated that the high density lipoprotein subfraction ratio (HDL2:HDL3) in females was significantly higher than in males. The cause of this phenomenon was investigated by simultaneous examination of the metabolism of the major HDL apoproteins (apoA-I and apoA-II) in both groups. The results show that there is no significant sex-related difference in the plasma pool size, fractional catabolic rate, or synthetic rate of either apoprotein. We conclude that the increased HDL2:HDL3 ratio in females versus males does not derive from measurable differences in the metabolic handling of either apoprotein.

Very low density lipoproteins (VLDL) and low density lipoproteins (LDL) from human normolipemic plasma, and the VLDL, the intermediate density lipoprotein (IDL), and LDL from patients with Type III hyperlipoproteinemic plasma were tested for their abilities to suppress the activity of 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase in cultured human fibroblasts from normal subjects and a Type III patient. Regulation of cholesterol synthesis in the fibroblasts of a patient with Type III hyperlipoproteinemia appears to be normal. VLDL from normal subjects, isolated by angle head ultracentrifugation (d < 1.006) or by gel filtration on BioGel A-5m, were about 5 times less effective than LDL in suppressing HMG-CoA reductase activity, based on protein content, in agreement with previous reports with normal fibroblasts. Zonal centrifugation of normal VLDL isolated by both methods showed that the VLDL contained IDL. Normal VLDL from the angle head rotor, refractionated by the zonal method, had little, if any, ability to suppress the HMG-CoA reductase activity in either normal or Type III fibroblasts. VLDL, IDL, and LDL fractionated by zonal ultracentrifugation from Type III plasma gave half-maximum inhibition at 0.2-0.5 mug of protein/ml, indistinguishable from the suppression caused by normal LDL. Type III VLDL did not suppress HMG-CoA reductase in mutant LDL receptor-negative fibroblasts. Zonally isolated VLDL obtained from one Type IV and one Type V patient gave half-maximal suppression at 5 and 0.5 mug of protein/ml, respectively. Molecular diameters and apoprotein compositions of the zonally isolated normal and Type III VLDL were similar; the major difference in composition was that Type III VLDL contained more cholesteryl esters and less triglyceride than did normal VLDL. The compositions and diameters of the Type IV and Type V VLDL were similar to normal VLDL. These findings show that the basic defect in Type III hyperlipoproteinemia is qualitatively different from the cellular defect found in familial hypercholesterolemia, since the regulation of HMG-CoA reductase activity is normal in Type III fibroblasts. The metabolic defect in hypertriglyceridemia is related to the triglyceriderich lipoproteins which, free of other lipoproteins, have an enhanced ability to interact with cultured fibroblasts to regulate HMG-CoA reductase activity. These studies suggest that, in hypertriglyceridemia, there is a mechanism for direct cellular catabolism of VLDL which is not functional for normal VLDL.

Subfractions of VLDL (VLDL 1 , Sf 100–400; VLDL 2 V Sf 60–100; VLDL 3 , Sf 20–60) and LDL (LDL 1 , Sf 12–20; LDL 2 , Sf 6–12; LDL 3 , Sf 3–6) were isolated from the plasma of three normal, three type in and four type IV hyperlipoproteinemic subjects. In the type IV group, all VLDL subspecies Were of normal composition but were increased in concentration in the order VLDL 1 > VLDL 2 > VLDL 3 . In the same subjects, although LDL 1 was lowered and LDL 3 increased, the total plasma LDL concentration Was normal. All VLDL subfractions were elevated in the type III group, but in this ease VLDL 3 predominated. These subfractions were enriched in cholesteryl esters and depleted in triglyceride. In the LDL density range there was a shift of mass towards the least dense fraction, LDL 1 , which was of normal composition. EPR studies of the VLDL and LDL subfractions in a type IV subject demonstrated a decrease in fluidity with increasing density. The major change occurred between VLDL 3 and LDL, and was attributed to a substantial alteration in the cholesteryl ester: triglyceride ratio in the particle. A similar argument was used to explain the reduced fluidity of type III VLDL 3 with respect to that of the same subfraction in normal or type IV subjects. Particle diameters, determined by laser light-scattering speetroscopy were-in good agreement with the values obtained by electron microscopy. This study provides a baseline for the examination of the relationship' between the physical and metabolic properties of VLDL and LDL subtractions in type in and IV hyperlipoproteinemia.

In this study we have investigated, in four normal males the effects of dietary saturated and polyunsaturated fat on the chemical composition and thermotropic properties of human high density lipoproteins (HDL) and have measured the influence of the diets on the metabolism of that fraction of HDL apolipoprotein A-I (apoA-I) that undergoes exchange in vitro and accounts for approximately two-thirds of the lipoprotein's apoA-I complement. When compared with the saturated fat diet, the polyunsaturated diet reduced plasma cholesterol (24%, P0.01) by affecting the cholesterol content in the very low density lipoprotein ( downward arrow25%, P0.02), low density lipoprotein ( downward arrow20%, P0.01), and high density lipoprotein fractions ( downward arrow33%, P0.01). Plasma triglyceride was also lowered (by 13%, P0.01). Furthermore, polyunsaturated fat ingestion caused a significant fall in the palmitate and stearate content of HDL triglyceride (41 and 37%, respectively), cholesteryl esters (29 and 35%), and phospholipids (17 and 9%) with a concomitant increase in the linoleate content of these moieties (157, 28, and 29%, respectively). The polyunsaturated diet also produced reciprocal changes in the percentage protein ( downward arrow9%, P0.02) and phospholipid ( downward arrow11.5%, P0.01) in HDl. These compositional changes were associated with an increase in the microscopic fluidity of the polyunsaturated HDL, although both diets had little effect on the fluidity parameters of HDL at body temperature. Rate zonal ultracentrifugation indicated that the HDL(2)/HDL(3) ratio fell by 28% (P0.05) on the polyunsaturated fat diet. In addition to the above, this diet reduced plasma apoA-I by 21% (P0.01). No change was seen in the fractional catabolic rate or the distribution of the apoprotein between intravascular and extravascular compartments on the two diets. However, when compared with the saturated diet, the synthetic rate of apoA-I was reduced by 26% during polyunsaturated fat feeding. The results show that polyunsaturated fat alters the chemical composition, thermotropic properties, and subfraction distribution of HDL without changing the fractional rate of catabolism of their major protein, apoA-I.These findings deserve careful consideration in determining the applicability and efficacy of polyunsaturated fat diet therapy in the prevention of atherosclerosis in man.

Symptomatic fasting and reactive hypoglycemia in association with low iejunal and hepatic fructose-1,6-diphosphatase (FDPase) activity were found in an adult patient and her 19-month-old daughter. Plasma glucose response to glycerol ingestion was abnormal in both, showing little change in the adult and a decline in the child. FDPase activity was low in tissues from both patients. This reduced enzyme activity defines a new variant of the fructose-1,6-dipphosphatase deficiency state(s) which is mild in its manifestations and thus more resistant to the hypoglycemia-provoking effects of fructose and alanine. No difference was detected between the FDPase from these patients and unaffected individuals by polyacrylamide gel electrophoresis. The K m of the jejunal enzyme for fructose diphosphate in the adult was similar to the normal control. Glucagon rapidly increased the activity of hepatic FDPase in both patients. Folic acid, 15 mg/day, increased hepatic and jejunal FDPase activity in both patients. After folate therapy, there was improvement in the adult's symptoms and improvement in the child's ability to maintain normal plasma glucose levels. The interrelationship between the hypoglycemia, FDPase deficiency, and the response to folate therapy was discussed.

Apolipoprotein B (apoB) metabolism was investigated in four normal, three type IV, and three type V hyperlipoproteinemic subjects. Following injection of autologous radioiodinated very low density lipoprotein (VLDL) the rate of clearance of the apoprotein from this particle and its subsequent appearance in low density lipoprotein (LDL) was measured by frequent apoB specific activity determinations over an 11-day period. The resultant data were analyzed using the SAAM 27 computer program. In the normal subjects, more than 95% of the injected VLDL apoB was rapidly transferred to the LDL density range and accounted for all LDL apoB synthesis in that group. The plasma VLDL apoB concentration in the type IV group was, on average, five times the normal level. This resulted primarily from a doubling of the VLDL apoB synthetic rate associated with a defective or saturated catabolic mechanism. Only 60% of this material subsequently appeared in LDL, while the remainder was catabolized via an LDL-independent pathway. The turnover parameters of LDL apoB were normal in the type IV patients. Type V hyperlipoproteinemic subjects exhibited a 12- to 35-fold increase in plasma VLDL apoB concentration over normal. This again derived from increased VLDL apoB synthesis in the presence of defective removal of the apoprotein; the fractional catabolic rate of VLDL apoB in this group was 14% of the normal value. However, in contrast to the type IV patient data, more than 85% of the apoB in type V VLDL eventually appeared in LDL whose turnover rate was raised as a result of an increase in its catabolism; the fractional catabolic rate of LDL apoB in type V patients was four-fold above normal. The plasma LDL apoB pool size was substantially reduced in these subjects. This study shows that in hyperlipoproteinemic pheno-types IV and V there exist multiple anomalies of apoB metabolism affecting both VLDL and LDL.

This report describes the effects of pharmacologic doses (3 g/d) of nicotinic acid on the plasma distribution and chemical composition of the high density lipoprotein (HDL) subfractions HDL2 and HDL3 and examines the influence of the drug on the metabolism of the major HDL apoproteins, apolipoproteins A-I (ApoA-I) and A-II (Apo-II). The drug lowered plasma cholesterol (15%, P < 0.05) and triglyceride (27%, P < 0.01); the former effect a result of a fall in the amount of cholesterol associated with very low density lipoproteins (31%, P < 0.02) and low density lipoproteins (36%, P < 0.02). Conversely, it raised plasma HDL cholesterol (23%, P < 0.05) and increased (by 345%) the plasma HDL2:HDL3 ratio. The latter derived from an absolute increment (646%) in circulating HDL2, coupled with a fall (47%) in HDL3. This change was not associated with major alterations in the overall cholesterol (free and esterified), triglyceride, phospholipid, or protein content of the subfractions; however, it was accompanied by substantial changes in their protein composition. In particular, the molar ratio of ApoA-I:ApoA-II in HDL3 declined from 2.7:1 to 2.1:1 during nicotinic acid treatment. Significant perturbations of ApoA-I and ApoA-II metabolism accompanied the drug-induced HDL subfraction redistribution. Specifically, the plasma concentration of ApoA-I rose by 7% (P < 0.05) because of a decrease in its fractional catabolic rate. Moreover, whereas before treatment 6 and 94% of the plasma ApoA-I circulated with HDL2 and HDL3, after commencement of nicotinic acid therapy this distribution became 49 and 51% in HDL2 and HDL3, respectively. ApoA-II was found mainly in HDL3, both before and during nicotinic acid treatment. Administration of the drug caused a 14% reduction in its plasma concentration (P < 0.05), which derived principally from a fall (22%, P < 0.01) in its synthetic rate. These data suggest that the effects of nicotinic acid on the HDL subfraction distribution may be mediated via (a) net transfer of ApoA-I from HDL3 to HDL2 and (b) a reduction in ApoA-II synthesis. Our present understanding of the association between HDL and atherosclerosis indicates that such changes may have prophylactic value in the prevention of coronary artery disease.

1. 1. Glucagon, insulin and epinephrine produce rapid changes (within minutes) in hepatic and jejunal formiminotransferase ( N- formimino- L -glutamate: tetrahydrofolate 5-formiminotransferase, EC 2.1.2.5) activity in the rat. Limited evidence in human subjects and patients demonstrate that similar effects also occur in man. 2. 2. Intravenous glucagon (0.0015–0.5 mg) and isnulin (0.015–1.5 units/kg) produced rapid increases and decreases, respectively, in hepatic formiminotransferase activity which were unaltered by pretreatment of the rats with either actinomycin D or puromycin. Intravenous insulin and glucagon significantly decreased and increased, respectively, rat jejunal formiminotransferase activity, as well. Intravenous epinephrine (1.0–2.0 μg/min) also produced a rapid increase in hepatic formiminotransferase activity. The stimulatory effects of glucagon and epinephrine on hepatic formiminotransferase activity were mimicked by intravenous cyclic AMP suggesting that these hormonal responses may be mediated through increased cyclic AMP production. 3. 3. In four children with fasting-induced hypoglycemia, intravenous glucagon (1 mg/min) significantly increased hepatic formiminotransferase activity within 2–3 min. In five normal human subjects, the administration of subcutaneous insulin (15 units) for three consecutive days significantly decreased jejunal formiminotransferase activity. 4. 4. We suggest that the rapid insulin, glucagon and epinephrine effects on formiminotransferase activity may be due to dephorylation— phosphorylation mechanisms analogous to that involved in the regulation of glycogen metabolism.

Glucagon and insulin given intravenously produced rapid reciprocal changes in the activity of phosphofructokinase (PFK), pyruvate kinase (PK) and fructose diphosphatase (FDPase) in rat liver. Fructose diphosphate aldolase did not change. Glucagon increased FDPase and decreased PFK and PK activity. Insulin decreased FDPase and increased PFK and PK activity. The glucagon effect was associated with a marked increase in hepatic cyclic adenosine 3′,5′-monophosphate (cyclic AMP) concentrations. Insulin did not significantly alter hepatic cyclic AMP concentrations.

A patient is reported who first sought treatment because of failing vision and hypogonadism secondary to a craniopharyngioma. This was surgically removed, and during the immediate postoperative period the patient developed hyperthermia in the absence of apparent infection, aphasia, hemiplegia and diabetes insipidus. He recovered from these and then became hypothyroid. About a year later, while taking desiccated thyroid, he developed hyperthyroidism with diffuse overactivity of the gland not suppressible with exogenous triiodothyronine. This sequence of events suggests that the hyperthyroidism resulted from an extrathyroidal disorder in this patient.

Metabolic studies of a 9-year-old girl with primary type V hyperlipoproteinemia demonstrated normal glucose tolerance, plasma insulin and glucagon responses to stimuli, and serum uric acid level. Fasting plasma triglyceride levels rapidly increased when the patient received a diet containing 40% of the total calories as fat and rapidly decreased on a 10% fat diet. Hepatic and nonhepatic lipase activities in postheparin plasma were normal, thus excluding type I hyperlipoproteinemia. Because of the potential complication of acute pancreatitis in this disorder, early diagnosis and prompt institution of diet therapy is important. ( JAMA 238:2518-2520, 1977)

Excerpt The goal of the American Board of Internal Medicine is to improve the quality of medical care in the United States. It does this by developing standards to ensure that the certified interni...