1. Distinct transcriptomic profile of small arteries of hypertensive patients with chronic kidney disease identified miR-338-3p targeting GPX3 and PTPRS
- Author
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Olga, Berillo, Ku-Geng, Huo, Chantal, Richer, Júlio C, Fraulob-Aquino, Marie, Briet, Mark L, Lipman, Daniel, Sinnett, Pierre, Paradis, and Ernesto L, Schiffrin
- Subjects
Glutathione Peroxidase ,Physiology ,Receptor-Like Protein Tyrosine Phosphatases, Class 2 ,Endothelial Cells ,Vascular System Injuries ,Phosphoric Monoester Hydrolases ,Mice ,MicroRNAs ,HEK293 Cells ,Hypertension ,Internal Medicine ,Animals ,Humans ,RNA, Messenger ,Renal Insufficiency, Chronic ,Transcriptome ,Cardiology and Cardiovascular Medicine ,Aorta - Abstract
Hypertension is associated with vascular injury, which contributes to end-organ damage. MicroRNAs regulating mRNAs have been shown to play a role in vascular injury in hypertensive mice. We aimed to identify differentially expressed microRNAs and their mRNA targets in small arteries of hypertensive patients with/without chronic kidney disease (CKD) to shed light on the pathophysiological molecular mechanisms of vascular remodeling.Normotensive individuals and hypertensive patients with/without CKD were recruited ( n = 15-16 per group). Differentially expressed microRNAs and mRNAs were identified uniquely associated with hypertension (microRNAs: 10, mRNAs: 68) or CKD (microRNAs: 68, mRNAs: 395), and in both groups (microRNAs: 2, mRNAs: 32) with a P less than 0.05 and a fold change less than or greater than 1.3 in subcutaneous small arteries ( n = 14-15). One of the top three differentially expressed microRNAs, miR-338-3p that was down-regulated in CKD, presented the best correlation between RNA sequencing and reverse transcription-quantitative PCR (RT-qPCR, R2 = 0.328, P 0.001). Profiling of human aortic vascular cells showed that miR-338-3p was mostly expressed in endothelial cells. Two of the selected top nine up-regulated miR-338-3p predicted targets, glutathione peroxidase 3 ( GPX3 ) and protein tyrosine phosphatase receptor type S ( PTPRS ), were validated with mimics by RT-qPCR in human aortic endothelial cells ( P 0.05) and by a luciferase assay in HEK293T cells ( P 0.05).A distinct transcriptomic profile was observed in gluteal subcutaneous small arteries of hypertensive patients with CKD. Down-regulated miR-338-3p could contribute to GPX3 and PTPRS up-regulation via the canonical microRNA targeting machinery in hypertensive patients with CKD.http://links.lww.com/HJH/C27.
- Published
- 2022