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Background Osteoporosis (OP) is a systemic bone disease characterised by decreased bone mass and deterioration of bone microarchitecture with increased brittleness and fracture risk. It associates high morbidity and mortality for patients and has a high impact on health expenditure. Bone marrow stromal mesenchymal stem cells (BM-MSC) give rise to osteoprogenitor cells and osteoblasts and influence bone homeostasis. However after their intravenous (i/v) infusion their osteotropism is limited. Our group has demonstrated that the exofucosylation of the CD44 membrane antigen in MSC improves their homing to bone tissue and that the infusion of these cells is safe in a murine model. Objectives To evaluate the safety of i/v infusion of fucosylated BM-MSC in patients with OP, and secondarily assess their ability to improve the course of the disease Methods 10 women between 50 and 75 years old diagnosed with osteoporosis with a low impact fracture will be included and treated i/v with autologous fucosylated BM-MSC. The first 4 patients were treated with a dose of 2 × 10 6 cells/kg body weight and the other 6 with 5 × 10 6 cells/kg body weight. A 24 month follow-up will be conducted to evaluate the rate of severe and non-serious adverse events and secondary endpoints (decreased fracture rate, pain scores, functional status and quality of life, biochemical indexes of bone metabolism, quantitative computed tomography for morphometric and mechanical analysis of bone quality, densitometry, and histomorphometry Results Seven patients have been recruited to date. Two left the study for lack of cell proliferation and appearance of a complex form in karyotype during the cell culture, respectively. The first 4 patients were successfully infused, and after a median follow-up of 3 months no related adverse effects have been observed, no new osteoporotic fractures have appeared, and the analogue pain scale score (EVA) shows a tendency to decrease of pain in 3 of the 4 patients. Conclusions Our preliminary data indicate that clinical and GMP-grade production of BM-MSC is feasible. We have not observed any short-term adverse effects associated with treatment in infused patients. Disclosure of Interest None declared

Background: Sudden Cardiac death is an important health care problem in European countries. Inherited cardiac diseases are common causes in young individuals. We aim to report the preliminary results from a regional programme of sudden cardiac death (Pheidippides study). Methods: We included 94 consecutive sudden death (SD) cases and resuscitated cardiac arrests (CA) evaluated from Jan 2009 to Jan 2013 at the Hospital. Postmortem study of SD cases and comprehensive clinical cardiac study in survivors of cardiac arrest were performed. Cases with known history of coronary artery were excluded. In cases with available tissue or blood sample genetic testing of related genes was performed. Results: 75 SD (67, 89% males, aged 39±15 years) and 19 CA (9, 47% males, aged 30±24 years). Diagnosis of the underlying pathology was achieved in 76 (81%), with 24 (31%) Hypertrophic Cardiomyopathy, 12 (16%) ischaemic coronary disease, 6 (8%) Arrhythmogenic Cardiomyopathy, 6 (8%) Idiopathic left ventricular hypertrophy, 4 (5%) long QT, 4 (5%) unclassified cardiomyopathies, 3 (4%) endomyocardial fibroelastosis, 3 (4%) aortic abnormalities, 2 (3%) Dilated Cardiomyopathy, 2 (3%) Chagas Cardiomyopathy, 1 (1.3%) cocaine abuse related SD, 1 (1.3%) Brugada syndrome,1 (1.3%) cardiac sarcoidosis, 1 (1.3%) coronary anomaly, 1 (1.3%) myocarditis,1 (1.3%) cathecolaminergic ventricular tachycardia, 1 (1.3%) conduction disease,1 (1.3%) malignant vasospasm, 1 (1.3%) malignant epilepsy and 1 (1.3%) Idiopathic ventricular fibrillation. Genetic diagnosis was achieved in 13 out of the 50 (26%) in which genetic testing guided by the pathology was carried out (ongoing study). Most prevalent genes were 5 desmosomal, 1 sarcomeric and 5 channels coding genes. Conclusion: There is a clear male predominance in SD and CA cases (80%) in our Region. Cardiomyopathies, in particular hypertrophic cardiomyopathy are the main cause of death. Despite postmortem in SD cases and dedicated clinical study in survivors a significant proportion (1 in 4) remain without a final diagnosis.