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Your search keyword '"Ferlazzo V"' showing total 4 results
Start Over Author "Ferlazzo V" Topic interleukins
4 results on '"Ferlazzo V"'

1. Interleukin-15, as Interferon-gamma, Induces the Killing ofLeishmania infantumin Phorbol-Myristate-Acetate-Activated Macrophages Increasing Interleukin-12.

Author

D'Agostino, P., Milano, S., Arcoleo, F., Di Bella, G., La Rosa, M., Ferlazzo, V., Caruso, R., Chifari, N., Vitale, G., Mansueto, S., and Cillari, E.

Subjects
LEISHMANIASIS, PROTOZOAN diseases, PARASITIC diseases, INTERLEUKINS, GROWTH factors, CYTOKINES
Abstract

The potential leishmanicidal activity of interleukin-15 (IL-15) was examined while priming with the cytokine phorbol-myristate-acetate (PMA)-activated macrophages and infecting them withLeishmania infantumparasites. The activation of macrophage cultures with IL-15 determined a significant anti-leishmanial activity, comparable with that induced by interferon-gamma (IFN-γ). The killing ofLeishmaniain macrophages primed with IL-15, as well as with IFN-γ, was followed by an increase in the IL-12 synthesis. The neutralization of IL-15 or IFN-γ, by specific monoclonal antibodies (MoAb) caused a significant reduction in leishmanicidal activity. Furthermore, in PMA-activated macrophages, the neutralization of IL-12 production by a specific anti-IL-12 MoAb reduced leishmanicidal activity induced by IL-15 and IFN-γ. Data indicate that IL-15 could have a role as an activator of leishmanicidal activity, directly or indirectly, by inducing IL-12 production. [ABSTRACT FROM AUTHOR]

Published
2004
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2. IL-15 in human visceral leishmaniasis caused by Leishmania infantum.

Author

MILANO, S., BELLA, G. DI, D’AGOSTINO, P., BARBERA, C., CARUSO, R., ROSA, M. LA, FERLAZZO, V., VITALE, G., RUSSA, C. LA, GAMBINO, G., CHIFARI, N., MANSUETO, S., and CILLARI, E.

Subjects
INTERLEUKINS, VISCERAL leishmaniasis, CYTOKINES, T cells
Abstract

SUMMARY Interleukin (IL)-15 is a recently discovered cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. Here, we investigated the involvement of IL-15 in the immune response to Leishmania infantum infection by studying patients with visceral leishmaniasis (VL). We found that IL-15 is produced by leishmanial antigen (LAg)-stimulated peripheral blood mononuclear cells (PBMC) from active VL patients at a significantly higher level than those produced by cells from healed VL subjects or healthy controls. A significant increase in IL-15 serum blood levels was also observed in acute VL patients compared with healed ones. Furthermore, recombinant IL-15 had an appreciable effect in vitro in reducing IL-4 and increasing the production of IL-12 in response to LAg, but it was ineffective in altering the production of interferon-γ (IFN-γ). The production of endogenous IL-15 in acute VL patients appeared to be insufficient to activate both IFN-γ and IL-12, as attested by the absence of modification of these two cytokines by neutralization experiments in the presence of anti-IL-15 monoclonal antibodies (MoAB). On the contrary, the neutralization of IL-15 increased IL-4 production. Together, these results indicate that endogenous IL-15 plays a role in the suppression of Th2-type cytokines, even though it does not enhance the production of Th1 cytokines in acute VL patients. Since IL-15, in the presence of anti-IL-4 MoAb, caused a further increase in IL-12 production and led to a significant production of IFN-γ, one of its indirect effects on Th1 cell activation could be due to the latter’s effect on Th2 cytokines such as IL-4. Therefore, our observations indicate that there is a potential for IL-15 to augment the T-cell response to human intracellular pathogens. [ABSTRACT FROM AUTHOR]

Published
2002
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3. Cytokines and growth factors in wound drainage fluid from patients undergoing incisional hernia repair

Author

DI VITA, Gaetano Giuseppe, PATTI, Rosalia, D'AGOSTINO P, CARUSO, Giuseppe, ARCARA, Matteo, Buscemi, Salvatore, BONVENTRE, Sebastiano, FERLAZZO, Viviana, ARCOLEO F, CILLARI E., DI VITA, G., Patti, R., D'Agostino, P., Caruso, G., Arcara, M., Buscemi, S., Bonventre, S., Ferlazzo, V., Arcoleo, F., and Cillari, E.

Subjects
Postoperative Care, Vascular Endothelial Growth Factor A, GRANULATION-TISSUE FORMATION, Interleukins, SKIN FLAPS, PROFILES, Exudates and Transudates, Middle Aged, Suction, VEGF, Hernia, Ventral, FACTOR EXPRESSION, EARLY SURGICAL WOUNDS, Interferon-gamma, COLORECTAL SURGERY, SURVIVAL, MEDIATES ANGIOGENIC ACTIVITY, Cytokines, Humans, Female, Fibroblast Growth Factor 2
Abstract

Knowing the dynamics of growth factor and cytokine secretion within the site of a surgical operation is important, as they play a crucial role in the pathophysiology of wound healing and are a target for modifying the repair response. The aim of this study was to evaluate the production of several cytokines and growth factors in the drainage wound fluid from patients undergoing incisional hernia repair: namely, interleukin (IL)-6, IL-10, IL-1alpha, IL-1 ra, interferon-gamma, vascular endothelial growth factors and basic fibroblast growth factor. Ten female patients with abdominal midline incisional hernia undergoing surgical repair were included in this study. In all cases, a closed-suction drain was inserted in the wound below the fascia and removed on postoperative day 4. Wound fluid was collected on postoperative days 1-4 and the amount was recorded each time. Growth factors and cytokines production was evaluated as the whole amount produced over a 24-hour period. In all patients, the amount of drain fluid from surgical wounds was more copious the first day after surgery, it decreased significantly afterward. The presence of all cytokines was highest on postoperative day 1, decreasing over the following days. More specifically, the production of IL-1 ra, IL-6, IL-1alpha, and IL-10 on postoperative day 1 fell sharply on postoperative days 3 and 4, whereas, after an initial reduction, interferon-gamma showed an increase from day 2 onward. Vascular endothelial-derived growth factor production increased progressively after the operation reaching statistical significance only on day 4. As for basic fibroblast growth factor, it showed an opposite pattern: it was higher on postoperative day 1 decreasing thereafter. This analysis of cytokine and growth factor production in the drain fluid will lead us to a better evaluation of the events that follow a surgical wound and to a better understanding of the healing process.

Published
2006

4. Modifications in the production of cytokines and growth factors in drainage fluids following mesh implantation after incisional hernia repair

Author

Pietro D'Agostino, Gaetano Di Vita, Mariangela Angileri, Matteo Arcara, Giuseppe Caruso, Rosalia Patti, Enrico Cillari, Viviana Ferlazzo, Gianluca Sieli, Salvatore Buscemi, DI VITA, G., Patti, R., D'Agostino, P., Ferlazzo, V., Angileri, M., Sieli, G., Buscemi, S., Caruso, G., Arcara, M., and Cillari, E.

Subjects
Adult, Vascular Endothelial Growth Factor A, medicine.medical_specialty, incisional hernia, Incisional hernia, medicine.medical_treatment, Enzyme-Linked Immunosorbent Assay, Polypropylenes, chemistry.chemical_compound, medicine, Humans, Hernia, Prospective Studies, Postoperative Care, Laparotomy, Pain, Postoperative, business.industry, Interleukins, Growth factor, Suture Techniques, growth factor, General Medicine, Middle Aged, Surgical Mesh, medicine.disease, Hernia repair, Hernia, Ventral, interieukin, mesh implantation, Surgery, Vascular endothelial growth factor, Treatment Outcome, Cytokine, Surgical mesh, chemistry, Cytokines, Female, Inflammation Mediators, Vascular endothelial growth factor production, business, Follow-Up Studies
Abstract

Background The aim of this study was to evaluate changes in the production of some cytokines (interleukins [ILs]-6, -10, -1, and -1ra), vascular endothelial growth factor, and beta-fibroblast growth factor after polypropylene mesh implantation. Methods Twenty female patients were divided into 2 groups. In 1 group, hernia repair was performed with conventional sutures (CR), whereas in the other group polypropylene mesh (MR) was used. Growth factors and cytokines production was analyzed in wound drain fluids based on the amount produced during 24 hours. Results IL-1 increased substantially in MR patients on postoperative days 1 and 2. IL1-ra and IL-10 production was always significantly higher in CR patients. IL-6 production did not show any considerable difference between the 2 groups. Vascular endothelial growth factor production was significantly higher in the MR than the CR group at all time points, whereas beta-fibroblast growth factor production was higher in the MR than the CR group only on postoperative day 1. Comments Our data suggest that different surgical procedures induce various levels of inflammation and that implantation of prostheses significantly stimulates the inflammatory response.

Published
2006

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