1. Interleukin-4 -590C/T gene polymorphism in Egyptian children with acute lower respiratory infection: A multicenter study.
- Author
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Emam AA, Shehab MMM, Allah MAN, Elkoumi MA, Abdelaal NM, Mosabah AAA, Zakaria MT, Sherif AM, Soliman MM, El-Kaffas RMH, Abouzeid H, Abdou MA, Abdalmonem N, Abdelbaset HR, Mohamed SA, Soliman AA, Elashkar SSA, Hegab MS, Khalil AM, Abdel-Aziz A, Anany HG, Salah HE, Abdou AM, Elshehawy NA, Elbasyouni HAA, Hafez SFM, Abo-Alella DA, Fawzi MM, and Morsi SS
- Subjects
- Alleles, Bronchiolitis blood, Child, Preschool, Egypt, Female, Genotype, Humans, Infant, Interleukin-4 blood, Male, Pneumonia blood, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Respiratory Tract Infections blood, Bronchiolitis genetics, Genetic Predisposition to Disease, Interleukin-4 genetics, Pneumonia genetics, Respiratory Tract Infections genetics
- Abstract
Background: Acute lower respiratory infection (ALRI) is the leading cause of child mortality, especially in the developing world. Polymorphisms in the interleukin 4 (IL-4) gene have been linked to a variety of human diseases., Objectives: To investigate whether the IL-4 -590C/T (rs2243250) polymorphism could be a genetic marker for susceptibility to ALRIs in young Egyptian children., Methods: This was a multicenter study conducted on 480 children diagnosed with pneumonia or bronchiolitis, and 480 well-matched healthy control children. Using PCR-RFLP analysis, we genotyped a -590C/T (rs2243250) single nucleotide polymorphism of the IL-4 gene promoter, meanwhile the serum IL-4concentration was measured by ELISA., Results: The frequency of the IL-4 -590 T/T genotype and T allele were overrepresented in patients with ALRIs in comparison to the control group (OR = 2.0; [95% confidence interval [CI]: 1.38-2.96]; for the T/T genotype) and (OR: 1.3; [95%CI: 1.07-1.56]; for the T allele; P < 0.01). The IL-4 -590 T/T genotype was associated with significantly higher mean serum IL-4 concentration (58.7 ± 13.4 pg/mL) compared to the C/T genotype (47.6 ± 11 pg/mL) and the C/C genotype (34.8 ± 9.6 pg/mL); P < 0.01., Conclusion: The IL-4 -590C/T (rs2243250) polymorphism may contribute to susceptibility to ALRIs in young Egyptian children., (© 2019 Wiley Periodicals, Inc.)
- Published
- 2019
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