10 results on '"O'Rourke, Robert A."'
Search Results
2. Adipose METTL14‐Elicited N6‐Methyladenosine Promotes Obesity, Insulin Resistance, and NAFLD Through Suppressing β Adrenergic Signaling and Lipolysis.
- Author
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Kang, Qianqian, Zhu, Xiaorong, Ren, Decheng, Ky, Alexander, MacDougald, Ormond A., O'Rourke, Robert W., and Rui, Liangyou
- Subjects
LIPOLYSIS ,INSULIN resistance ,NON-alcoholic fatty liver disease ,HIGH-fat diet ,WHITE adipose tissue ,SYMPATHETIC nervous system - Abstract
White adipose tissue (WAT) lipolysis releases free fatty acids as a key energy substance to support metabolism in fasting, cold exposure, and exercise. Atgl, in concert with Cgi‐58, catalyzes the first lipolytic reaction. The sympathetic nervous system (SNS) stimulates lipolysis via neurotransmitter norepinephrine that activates adipocyte β adrenergic receptors (Adrb1‐3). In obesity, adipose Adrb signaling and lipolysis are impaired, contributing to pathogenic WAT expansion; however, the underling mechanism remains poorly understood. Recent studies highlight importance of N6‐methyladenosine (m6A)‐based RNA modification in health and disease. METTL14 heterodimerizes with METTL3 to form an RNA methyltransferase complex that installs m6A in transcripts. Here, this work shows that adipose Mettl3 and Mettl14 are influenced by fasting, refeeding, and insulin, and are upregulated in high fat diet (HFD) induced obesity. Adipose Adrb2, Adrb3, Atgl, and Cgi‐58 transcript m6A contents are elevated in obesity. Mettl14 ablation decreases these transcripts' m6A contents and increases their translations and protein levels in adipocytes, thereby increasing Adrb signaling and lipolysis. Mice with adipocyte‐specific deletion of Mettl14 are resistant to HFD‐induced obesity, insulin resistance, glucose intolerance, and nonalcoholic fatty liver disease (NAFLD). These results unravel a METTL14/m6A/translation pathway governing Adrb signaling and lipolysis. METTL14/m6A‐based epitranscriptomic reprogramming impairs adipose Adrb signaling and lipolysis, promoting obesity, NAFLD, and metabolic disease. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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3. Lumican modulates adipocyte function in obesity-associated type 2 diabetes.
- Author
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Strieder-Barboza, Flesher, Carmen G., Geletka, Lynn M., Eichler, Tad, Akinleye, Olukemi, Ky, Alexander, Ehlers, Anne P., Lumeng, Carey N., and O'Rourke, Robert W.
- Subjects
TYPE 2 diabetes ,FAT cells ,ADIPOSE tissues ,INSULIN sensitivity ,INSULIN resistance - Abstract
Obesity-associated type 2 diabetes (DM) leads to adipose tissue dysfunction. Lumican is a proteoglycan implicated in obesity, insulin resistance (IR), and adipocyte dysfunction. Using human visceral adipose tissue (VAT) from subjects with and without DM, we studied lumican effects on adipocyte function. Lumican was increased in VAT and adipocytes in DM. Lumican knockdown in adipocytes decreased lipolysis and improved adipogenesis and insulin sensitivity in VAT adipocytes in DM, while treatment with human recombinant lumican increased lipolysis and impaired insulin-sensitivity in an ERK-dependent manner. We demonstrate that lumican impairs adipocyte metabolism, partially via ERK signalling, and is a potential target for developing adipose tissue-targeted therapeutics in DM. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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4. Regulation of adipose tissue inflammation and systemic metabolism in murine obesity by polymer implants loaded with lentiviral vectors encoding human interleukin‐4.
- Author
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Youngblood, Richard, Flesher, Carmen G., Delproposto, Jennifer, Baker, Nicki A., Neeley, Christopher K., Li, Fanghua, Lumeng, Carey N., Shea, Lonnie D., and O'Rourke, Robert W.
- Abstract
Dysfunctional adipose tissue plays a central role in the pathogenesis of the obesity‐related metabolic disease, including type 2 diabetes. Targeting adipose tissue using biopolymer implants is a novel therapeutic approach for metabolic disease. We transplanted porous poly(lactide‐co‐glycolide) (PLG) implants coated with human interleukin‐4 (hIL‐4)‐expressing lentivirus into epididymal white adipose tissue (eWAT) of mice fed a high‐fat diet. Tissue and systemic inflammation and metabolism were studied with flow cytometry, immunohistochemistry, quantitative real‐time polymerase chain reaction, adipose tissue histology, and in vivo glucose tolerance testing at 2 and 10 weeks of a high‐fat diet. PLG implants carrying hIL‐4‐expressing lentivirus implanted into epididymal white adipose tissue of mice‐regulated adipose tissue inflammation, including increased CD3+CD4+ T‐cell frequency, increased eWAT adipocyte hypertrophy, and decreased FASN and ATGL expression, along with reduced fasting blood glucose levels. These effects were observed in early obesity but were not maintained in established obesity. Local delivery of bioimplants loaded with cytokine‐expressing lentivirus vectors to adipose tissue influences tissue inflammation and systemic metabolism in early obesity. Further study will be required to show more durable metabolic effects. These data demonstrate that polymer biomaterials implanted into adipose tissue have the potential to modulate local tissue and systemic inflammation and metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
5. Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity
- Author
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O’Rourke, Robert W., Meyer, Kevin A., Neeley, Christopher K., Gaston, Garen D., Sekhri, Palak, Szumowski, Marek, Zamarron, Brian, Lumeng, Carey N., and Marks, Daniel L.
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Inflammation ,obesity ,Macrophages ,T-Lymphocytes ,Subcutaneous Fat ,Mice, Obese ,Mice, Transgenic ,NK cells ,Intra-Abdominal Fat ,Article ,Killer Cells, Natural ,Mice, Inbred C57BL ,NKp46 ,Mice ,insulin resistance ,Animals ,Cytokines ,Insulin - Abstract
Objective NK cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity. Methods A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet-induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing. Results NK cell ablation achieved a 3-4 fold decrease in NK cells but had no effect on T-cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra-abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines. Conclusion NK cells play a role in promoting intra-abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity.
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- 2014
6. Depot-specific adipocyte-extracellular matrix metabolic crosstalk in murine obesity.
- Author
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Strieder-Barboza, Clarissa, Baker, Nicki A., Flesher, Carmen G., Karmakar, Monita, Patel, Ayush, Lumeng, Carey N., and O'Rourke, Robert W.
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ADIPOSE tissues ,TISSUE culture ,METABOLIC disorders ,OBESITY ,EXTRACELLULAR matrix - Abstract
Subcutaneous (SAT) and visceral (VAT) adipose tissues have distinct metabolic phenotypes. We hypothesized that the extracellular matrix (ECM) regulates depot-specific differences in adipocyte metabolic function inmurine obesity. VAT and SAT preadipocytes from lean or obese mice were subject to adipogenic differentiation in standard 2D culture on plastic tissue culture plates or in 3D culture in ECM, followed by metabolic profiling. Adipocytes from VAT relative to SATmanifested impaired insulin-stimulated glucose uptake and decreased adipogenic capacity. In 3D-ECM-adipocyte culture, ECM regulated adipocyte metabolism in a depot-specific manner, with SAT ECM rescuing defects in glucose uptake and adipogenic gene expression in VAT adipocytes, while VAT ECM impaired adipogenic gene expression in SAT adipocytes. These findings demonstrate that ECM-adipocyte crosstalk regulates depot-specific differences in adipocyte metabolic dysfunction in murine obesity. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Advanced glycation end-products regulate extracellular matrix-adipocyte metabolic crosstalk in diabetes.
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Strieder-Barboza, Clarissa, Baker, Nicki A., Flesher, Carmen G., Karmakar, Monita, Neeley, Christopher K., Polsinelli, Dominic, Dimick, Justin B., Finks, Jonathan F., Ghaferi, Amir A., Varban, Oliver A., Lumeng, Carey N., and O'Rourke, Robert W.
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EXTRACELLULAR matrix ,BIOLOGICAL crosstalk ,ADIPOSE tissue physiology ,INSULIN resistance ,DIABETES complications - Abstract
The adipose tissue extracellular matrix (ECM) regulates adipocyte cellular metabolism and is altered in obesity and type 2 diabetes, but mechanisms underlying ECM-adipocyte metabolic crosstalk are poorly defined. Advanced glycation end-product (AGE) formation is increased in diabetes. AGE alter tissue function via direct effects on ECM and by binding scavenger receptors on multiple cell types and signaling through Rho GTPases. Our goal was to determine the role and underlying mechanisms of AGE in regulating human ECM-adipocyte metabolic crosstalk. Visceral adipocytes from diabetic and non-diabetic humans with obesity were studied in 2D and 3D-ECM culture systems. AGE is increased in adipose tissue from diabetic compared to non-diabetic subjects. Glycated collagen 1 and AGE-modified ECM regulate adipocyte glucose uptake and expression of AGE scavenger receptors and Rho signaling mediators, including the DIAPH1 gene, which encodes the human Diaphanous 1 protein (hDia1). Notably, inhibition of hDia1, but not scavenger receptors RAGE or CD36, attenuated AGE-ECM inhibition of adipocyte glucose uptake. These data demonstrate that AGE-modification of ECM contributes to adipocyte insulin resistance in human diabetes, and implicate hDia1 as a potential mediator of AGE-ECM-adipocyte metabolic crosstalk. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Systemic NK cell ablation attenuates intra-abdominal adipose tissue macrophage infiltration in murine obesity.
- Author
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O'Rourke, Robert W., Meyer, Kevin A., Neeley, Christopher K., Gaston, Garen D., Sekhri, Palak, Szumowski, Marek, Zamarron, Brian, Lumeng, Carey N., and Marks, Daniel L.
- Subjects
KILLER cells ,OBESITY ,ETIOLOGY of diseases ,ADIPOSE tissues ,INSULIN resistance - Abstract
Objective Natural killer (NK) cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity. Methods A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet-induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing. Results NK cell ablation achieved a three to fourfold decrease in NK cells but had no effect on T-cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra-abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines. Conclusions NK cells play a role in promoting intra-abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
9. Systemic inflammation and insulin sensitivity in obese IFN-γ knockout mice.
- Author
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O'Rourke, Robert W., White, Ashley E., Metcalf, Monja D., Winters, Brian R., Diggs, Brian S., Zhu, Xinxia, and Marks, Daniel L.
- Subjects
INFLAMMATION ,KNOCKOUT mice ,ADIPOSE tissues ,MACROPHAGES ,INSULIN resistance ,OBESITY ,CELL fractionation ,HIGH-fat diet - Abstract
Abstract: Adipose tissue macrophages are important mediators of inflammation and insulin resistance in obesity. IFN-γ is a central regulator of macrophage function. The role of IFN-γ in regulating systemic inflammation and insulin resistance in obesity is unknown. We studied obese IFN-γ knockout mice to identify the role of IFN-γ in regulating inflammation and insulin sensitivity in obesity. IFN-γ-knockout C57Bl/6 mice and wild-type control litter mates were maintained on normal chow or a high fat diet for 13 weeks and then underwent insulin sensitivity testing then sacrifice and tissue collection. Flow cytometry, intracellular cytokine staining, and QRTPCR were used to define tissue lymphocyte phenotype and cytokine expression profiles. Adipocyte size was determined from whole adipose tissue explants examined under immunofluorescence microscopy. Diet-induced obesity induced systemic inflammation and insulin resistance, along with a pan-leukocyte adipose tissue infiltrate that includes macrophages, T-cells, and NK cells. Obese IFN-γ-knockout animals, compared with obese wild-type control animals, demonstrate modest improvements in insulin sensitivity, decreased adipocyte size, and an M2-shift in ATM phenotype and cytokine expression. These data suggest a role for IFN-γ in the regulation of inflammation and glucose homeostasis in obesity though multiple potential mechanisms, including effects on adipogenesis, cytokine expression, and macrophage phenotype. [Copyright &y& Elsevier]
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- 2012
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10. Adipose Tissue Dendritic Cells Are Independent Contributors to Obesity-Induced Inflammation and Insulin Resistance.
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Kae Won Cho, Zamarron, Brian F., Muir, Lindsey A., Singer, Kanakadurga, Porsche, Cara E., DelProposto, Jennifer B., Geletka, Lynn, Meyer, Kevin A., O'Rourke, Robert W., and Lumeng, Carey N.
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OBESITY , *ADIPOSE tissues , *DENDRITIC cells , *INFLAMMATION , *INSULIN resistance , *IMMUNOSTAINING - Abstract
Dynamic changes of adipose tissue leukocytes, including adipose tissue macrophage (ATM) and adipose tissue dendritic cells (ATDCs), contribute to obesity-induced inflammation and metabolic disease. However, clear discrimination between ATDC and ATM in adipose tissue has limited progress in the field of immunometabolism. In this study, we use CD64 to distinguish ATM and ATDC, and investigated the temporal and functional changes in these myeloid populations during obesity. Flow cytometry and immunostaining demonstrated that the definition of ATM as F4/80+CD11b+ cells overlaps with other leukocytes and that CD45+CD64+ is specific for ATM. The expression of core dendritic cell genes was enriched in CD11c+CD64- cells (ATDC), whereas core macrophage genes were enriched in CD45+CD64+ cells (ATM). CD11c+CD64- ATDCs expressed MHC class II and costimulatory receptors, and had similar capacity to stimulate CD4+ T cell proliferation as ATMs. ATDCs were predominantly CD11b+ conventional dendritic cells and made up the bulk of CD11c+ cells in adipose tissue with moderate high-fat diet exposure. Mixed chimeric experiments with Ccr2-/- mice demonstrated that high-fat diet-induced ATM accumulation from monocytes was dependent on CCR2, whereas ATDC accumulation was less CCR2 dependent. ATDC accumulation during obesity was attenuated in Ccr7-/- mice and was associated with decreased adipose tissue inflammation and insulin resistance. CD45+CD64+ ATM and CD45+CD64-CD11c+ ATDCs were identified in human obese adipose tissue and ATDCs were increased in s.c. adipose tissue compared with omental adipose tissue. These results support a revised strategy for unambiguous delineation of ATM and ATDC, and suggest that ATDCs are independent contributors to adipose tissue inflammation during obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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