21 results on '"Takahashi, Shin"'
Search Results
2. Elaidate, a trans fatty acid, suppresses insulin signaling for glucose uptake in a manner distinct from that of stearate.
- Author
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Ishibashi K, Takeda Y, Nakata L, Hakuno F, Takahashi SI, and Atsumi GI
- Subjects
- 3T3-L1 Cells, Adipocytes drug effects, Adipocytes metabolism, Adipocytes ultrastructure, Animals, Carbohydrate Metabolism drug effects, Cell Membrane metabolism, Glucose Transporter Type 4 metabolism, Mice, Oleic Acids chemistry, Phosphorylation drug effects, Protein Transport drug effects, Proto-Oncogene Proteins c-akt metabolism, Stearates chemistry, Transport Vesicles drug effects, Glucose metabolism, Insulin metabolism, Oleic Acids pharmacology, Signal Transduction drug effects, Stearates pharmacology
- Abstract
The dietary intake of elaidate (elaidic acid), a trans-fatty acid, is associated with the development of various diseases. Since elaidate is a C18 unsaturated fatty acid with a steric structure similar to that of a C18 saturated fatty acid (stearate), we previously revealed that insulin-dependent glucose uptake was impaired in adipocytes exposed to elaidate prior to and during differentiation similar to stearate. However, it is still unknown whether the mechanism of impairment of insulin-dependent glucose uptake due to elaidate is similar to that of stearate. Here, we indicate that persistent exposure to elaidate has particular effects on insulin signaling and GLUT4 dynamics. Insulin-induced accumulation of Akt at the plasma membrane (PM) and elevations of phosphorylated Akt and AS160 levels in whole cells were suppressed in adipocytes persistently exposed to 50 μM elaidate. Interestingly, persistent exposure to the same concentration of stearate has no effect on the phosphorylated Akt and AS160 levels. When cells were exposed to these fatty acids, elaidate suppressed insulin-induced fusion, but not translocation, of GLUT4 storage vesicles in the PM, whereas stearate did not suppress the fusion and translocation of GLUT4 storage, indicating that elaidate has suppressive effects on the accumulation of Akt and fusion of GLUT4 storage vesicles and that both elaidate and stearate vary in the mechanisms by which they impair insulin-dependent glucose uptake., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
- Published
- 2020
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3. Triglyceride synthesis in hepatocytes isolated from rats fed a low-protein diet is enhanced independently of upregulation of insulin signaling.
- Author
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Taguchi Y, Toyoshima Y, Tokita R, Kato H, Takahashi SI, and Minami S
- Subjects
- Animals, Cells, Cultured, Fatty Liver etiology, Fatty Liver metabolism, Male, Rats, Rats, Wistar, Diet, Protein-Restricted adverse effects, Hepatocytes metabolism, Insulin metabolism, Lipogenesis, Signal Transduction, Triglycerides metabolism
- Abstract
It is known that protein malnutrition develops fatty liver in rats. However, the mechanisms by which protein malnutrition enhances lipid accumulation in the liver are not fully understood. Our previous studies have demonstrated that protein malnutrition upregulates insulin signaling with an increase in TG levels in rat livers. Here, we examined whether the upregulated insulin signaling contributes to an enhancement of TG accumulation under protein malnutrition. As it is difficult to analyze insulin-induced hepatic TG synthesis in vivo, the isolated hepatocytes derived from rats fed a low-protein diet were used. The hepatocytes were isolated from rats fed a 15% casein diet (15C) as a control diet or a 5% casein diet (5C) as a low-protein diet and then treated with insulin. As shown in vivo, insulin signaling was upregulated in isolated hepatocytes from 5C-fed rats (5C hepatocytes). However, the insulin-induced increase in the mRNA levels of lipogenic enzymes, including acetyl-CoA carboxylase 1 (ACC1) and fatty acid synthase (FAS), was similar in both groups. The amounts of TG synthesized from both glucose and palmitate, as well as ACC1 and FAS protein levels, were increased at the basal state in 5C hepatocytes, but were not further increased by insulin. These results indicate that TG synthesis via both de novo fatty acid synthesis and esterification is enhanced in 5C hepatocytes, which is independent of the upregulation of insulin signaling., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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4. Tumor necrosis factor (TNF)-α-induced repression of GKAP42 protein levels through cGMP-dependent kinase (cGK)-Iα causes insulin resistance in 3T3-L1 adipocytes.
- Author
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Ando Y, Shinozawa Y, Iijima Y, Yu BC, Sone M, Ooi Y, Watanaka Y, Chida K, Hakuno F, and Takahashi S
- Subjects
- 3T3-L1 Cells, Adaptor Proteins, Signal Transducing genetics, Adipocytes cytology, Adipocytes metabolism, Animals, CHO Cells, Cricetinae, Cricetulus, Cyclic GMP-Dependent Protein Kinase Type I genetics, Drug Resistance, Glucose Transporter Type 4 metabolism, HEK293 Cells, Humans, Hypoglycemic Agents pharmacology, Immunoblotting, Insulin Receptor Substrate Proteins genetics, Insulin Receptor Substrate Proteins metabolism, Mice, Microscopy, Confocal, Phosphorylation drug effects, Protein Binding, Protein Transport drug effects, RNA Interference, Two-Hybrid System Techniques, Tyrosine genetics, Tyrosine metabolism, Adaptor Proteins, Signal Transducing metabolism, Adipocytes drug effects, Cyclic GMP-Dependent Protein Kinase Type I metabolism, Insulin pharmacology, Tumor Necrosis Factor-alpha pharmacology
- Abstract
Insulin receptor substrates (IRSs) have been shown to be major mediators of insulin signaling. Recently, we found that IRSs form high-molecular weight complexes, and here, we identify by yeast two-hybrid screening a novel IRS-1-associated protein: a 42-kDa cGMP-dependent protein kinase-anchoring protein (GKAP42). GKAP42 knockdown in 3T3-L1 adipocytes suppressed insulin-dependent IRS-1 tyrosine phosphorylation and downstream signaling, resulting in suppression of GLUT4 translocation to plasma membrane induced by insulin. In addition, GLUT4 translocation was also suppressed in cells overexpressing GKAP42-N (the IRS-1 binding region of GKAP42), which competed with GKAP42 for IRS-1, indicating that GKAP42 binding to IRS-1 is required for insulin-induced GLUT4 translocation. Long term treatment of 3T3-L1 adipocytes with TNF-α, which induced insulin resistance, significantly decreased the GKAP42 protein level. We then investigated the roles of cGMP-dependent kinase (cGK)-Iα, which bound to GKAP42, in these changes. cGK-Iα knockdown partially rescued TNF-α-induced decrease in GKAP42 and impairment of insulin signals. These data indicated that TNF-α-induced repression of GKAP42 via cGK-Iα caused reduction of insulin-induced IRS-1 tyrosine phosphorylation at least in part. The present study describes analysis of the novel TNF-α-induced pathway, cGK-Iα-GKAP42, which regulates insulin-dependent signals and GLUT4 translocation., (© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2015
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5. Aspp2 negatively regulates body growth but not developmental timing by modulating IRS signaling in zebrafish embryos.
- Author
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Liu C, Luan J, Bai Y, Li Y, Lu L, Liu Y, Hakuno F, Takahashi S, Duan C, and Zhou J
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Animals, Ankyrins metabolism, Apoptosis physiology, Apoptosis Regulatory Proteins genetics, Body Size physiology, Evolution, Molecular, HEK293 Cells, Humans, Proto-Oncogene Proteins c-akt metabolism, Somatomedins metabolism, Somites embryology, Somites physiology, Zebrafish genetics, Zebrafish Proteins, src Homology Domains physiology, Adaptor Proteins, Signal Transducing genetics, Gene Expression Regulation, Developmental physiology, Insulin metabolism, Insulin Receptor Substrate Proteins metabolism, MAP Kinase Signaling System physiology, Zebrafish embryology
- Abstract
The growth and developmental rate of developing embryos and fetus are tightly controlled and coordinated to maintain proper body shape and size. The insulin receptor substrate (IRS) proteins, key intracellular transducers of insulin and insulin-like growth factor signaling, play essential roles in the regulation of growth and development. A short isoform of apoptosis-stimulating protein of p53 2 (ASPP2) was recently identified as a binding partner of IRS-1 and IRS-2 in mammalian cells in vitro. However, it is unclear whether ASPP2 plays any role in vertebrate embryonic growth and development. Here, we show that zebrafish Aspp2a and Aspp2b negatively regulate embryonic growth without affecting developmental rate. Human ASPP2 had similar effects on body growth in zebrafish embryos. Aspp2a and 2b inhibit Akt signaling. This inhibition was reversed by coinjection of myr-Akt1, a constitutively active form of Akt1. Zebrafish Aspp2a and Aspp2b physically bound with Irs-1, and the growth inhibitory effects of ASPP2/Aspp2 depend on the presence of their ankyrin repeats and SH3 domains. These findings uncover a novel role of Aspp2 in regulating vertebrate embryonic growth., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2014
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6. Tissue-specific effects of protein malnutrition on insulin signaling pathway and lipid accumulation in growing rats.
- Author
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Toyoshima Y, Tokita R, Taguchi Y, Akiyama-Akanishi N, Takenaka A, Kato H, Chida K, Hakuno F, Minami S, and Takahashi S
- Subjects
- Animals, Dietary Proteins metabolism, Dietary Proteins pharmacology, Liver drug effects, Liver metabolism, Male, Malnutrition physiopathology, Organ Specificity, Rats, Rats, Wistar, Signal Transduction drug effects, Diet, Protein-Restricted adverse effects, Growth and Development drug effects, Insulin metabolism, Lipid Metabolism drug effects, Malnutrition metabolism
- Abstract
Our previous studies have revealed that protein malnutrition enhances insulin signaling in rat liver and muscle in response to a bolus insulin injection. However, it has not been established whether protein malnutrition up-regulates insulin signaling under physiological conditions, such as feeding. Here, we studied the effects of protein malnutrition on insulin signaling after feeding in rat liver, muscle and white adipose tissue (WAT). Six-week-old rats were fed a 15% casein diet (15C) or a calorie-matched 5% casein diet (5C) for 8 h/day during 14 days. On the 15th day, blood and tissues were collected at various time points after feeding. Feeding-induced insulin secretion was reduced in 5C-fed rats compared to 15C-fed rats. The 5C-feeding suppressed immediate activation of insulin receptor after feeding in the liver, muscle, and WAT. However, 5C-feeding constantly increased tyrosine phosphorylation of insulin receptor substrate (IRS)-2 and threonine phosphorylation of eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) in the liver during the examined periods, corresponding to the changes of their amounts. In skeletal muscle, 5C-feeding did not appreciably alter insulin signaling. In WAT, 5C-feeding decreased tyrosine phosphorylation of IRS-1 compared to 15C-feeding. Furthermore, hepatic triglyceride content was increased and feeding-induced acetyl-CoA carboxylase 1 gene expression was enhanced in 5C-fed rats. The 5C-feeding decreased insulin-dependent glucose uptake in adipocytes. These results suggest that enhanced insulin signaling through increased IRS-2 and 4E-BP1 levels in the liver and repressed insulin signaling through decreased IRS-1 levels in WAT contribute to the preferential hepatic lipid accumulation under protein malnutrition.
- Published
- 2014
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7. Insulin injection restored increased insulin receptor substrate (IRS)-2 protein during short-term protein restriction but did not affect reduced insulin-like growth factor (IGF)-I mRNA or increased triglyceride accumulation in the liver of rats.
- Author
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Ozaki Y, Takeda T, Akanishi N, Hakuno F, Toyoshima Y, Takahashi S, and Takenaka A
- Subjects
- Animals, Cell Line, Tumor, Injections, Insulin metabolism, Insulin Receptor Substrate Proteins blood, Insulin Secretion, Insulin-Like Growth Factor I metabolism, Liver drug effects, Male, Phosphorylation drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Rats, Wistar, Signal Transduction drug effects, Dietary Proteins analysis, Insulin administration & dosage, Insulin pharmacology, Insulin Receptor Substrate Proteins metabolism, Insulin-Like Growth Factor I genetics, Liver metabolism, Triglycerides metabolism
- Abstract
Dietary protein restriction reduces insulin-like growth factor (IGF)-I synthesis and impairs growth. Moreover, insulin secretion is impaired and hepatic insulin signaling is activated presumably through upregulation of insulin receptor substrate (IRS)-2, which can stimulate lipogenesis thereby resulting in steatosis. In order to determine whether impaired insulin secretion is the primary cause of these changes, we injected insulin into protein-restricted rats and compensated for the reduction in insulin secretion for 1 and 7 d. Insulin infusion did not overcome the reduction in liver IGF-I mRNA nor the hepatic triglyceride accumulation. In contrast, it clearly suppressed the upregulation of hepatic IRS-2 on day 1, but not on day 7. Furthermore, insulin elimination increased IRS-2 in H4IIE-C3 cells. In summary, we found that reduced insulin secretion during protein restriction directly increased hepatic IRS-2 as a rapid response on day 1, while additional mechanisms contributed to the upregulation of IRS-2 on day 7.
- Published
- 2014
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8. Par14 protein associates with insulin receptor substrate 1 (IRS-1), thereby enhancing insulin-induced IRS-1 phosphorylation and metabolic actions.
- Author
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Zhang J, Nakatsu Y, Shinjo T, Guo Y, Sakoda H, Yamamotoya T, Otani Y, Okubo H, Kushiyama A, Fujishiro M, Fukushima T, Tsuchiya Y, Kamata H, Iwashita M, Nishimura F, Katagiri H, Takahashi S, Kurihara H, Uchida T, and Asano T
- Subjects
- Animals, Binding Sites genetics, Glucose Intolerance genetics, HEK293 Cells, Hep G2 Cells, Humans, Hyperglycemia genetics, Hyperglycemia therapy, Hypoglycemic Agents pharmacology, Immunoblotting, Insulin Receptor Substrate Proteins genetics, Liver metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Obese, Mutation, NIMA-Interacting Peptidylprolyl Isomerase, Obesity blood, Obesity genetics, Peptidylprolyl Isomerase genetics, Phosphatidylinositol 3-Kinases metabolism, Phosphorylation drug effects, Protein Binding, Proto-Oncogene Proteins c-akt metabolism, RNA Interference, Insulin pharmacology, Insulin Receptor Substrate Proteins metabolism, Peptidylprolyl Isomerase metabolism
- Abstract
Pin1 and Par14 are parvulin-type peptidyl-prolyl cis/trans isomerases. Although numerous proteins have been identified as Pin1 substrates, the target proteins of Par14 remain largely unknown. Par14 expression levels are increased in the livers and embryonic fibroblasts of Pin1 KO mice, suggesting a compensatory relationship between the functions of Pin1 and Par14. In this study, the association of Par14 with insulin receptor substrate 1 (IRS-1) was demonstrated in HepG2 cells overexpressing both as well as endogenously in the mouse liver. The analysis using deletion-mutated Par14 and IRS-1 constructs revealed the N-terminal portion containing the basic domain of Par14 and the two relatively C-terminal portions of IRS-1 to be involved in these associations, in contrast to the WW domain of Pin1 and the SAIN domain of IRS-1. Par14 overexpression in HepG2 markedly enhanced insulin-induced IRS-1 phosphorylation and its downstream events, PI3K binding with IRS-1 and Akt phosphorylation. In contrast, treating HepG2 cells with Par14 siRNA suppressed these events. In addition, overexpression of Par14 in the insulin-resistant ob/ob mouse liver by adenoviral transfer significantly improved hyperglycemia with normalization of hepatic PEPCK and G6Pase mRNA levels, and gene suppression of Par14 using shRNA adenovirus significantly exacerbated the glucose intolerance in Pin1 KO mice. Therefore, although Pin1 and Par14 associate with different portions of IRS-1, the prolyl cis/trans isomerization in multiple sites of IRS-1 by these isomerases appears to be critical for efficient insulin receptor-induced IRS-1 phosphorylation. This process is likely to be one of the major mechanisms regulating insulin sensitivity and also constitutes a potential therapeutic target for novel insulin-sensitizing agents.
- Published
- 2013
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9. Insulin/insulin-like growth factor (IGF) stimulation abrogates an association between a deubiquitinating enzyme USP7 and insulin receptor substrates (IRSs) followed by proteasomal degradation of IRSs.
- Author
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Yoshihara H, Fukushima T, Hakuno F, Saeki Y, Tanaka K, Ito A, Yoshida M, Iemura S, Natsume T, Asano T, Chida K, Girnita L, and Takahashi S
- Subjects
- HEK293 Cells, Humans, Insulin pharmacology, Insulin-Like Growth Factor I pharmacology, Phosphatidylinositol 3-Kinases metabolism, Protein Stability, Proteolysis, RNA, Small Interfering genetics, Ubiquitin Thiolesterase genetics, Ubiquitin-Specific Peptidase 7, Insulin metabolism, Insulin Receptor Substrate Proteins metabolism, Insulin-Like Growth Factor I metabolism, Proteasome Endopeptidase Complex metabolism, Ubiquitin Thiolesterase metabolism
- Abstract
Insulin receptor substrates (IRSs) play central roles in insulin/insulin-like growth factor (IGF) signaling and mediate a variety of their bioactivities. IRSs are tyrosine-phosphorylated by activated insulin receptor/IGF-I receptor tyrosine kinase in response to insulin/IGF, and are recognized by signaling molecules possessing the SH2 domain such as phosphatidylinositol 3-kinase (PI3K), leading to the activation of downstream pathways. Recent studies have suggested that degradation of IRSs by the ubiquitin-proteasome pathway leads to impaired insulin/IGF signaling, but the precise mechanism underlying the process is still unclear. In this study, we identified deubiquitinating enzyme ubiquitin specific protease 7 (USP7) as an IRS-2-interacting protein and demonstrated that deubiquitinase activity of USP7 plays important roles in IRS-2 stabilization through the ubiquitin-proteasome pathway. In addition, insulin treatment dissociated USP7 from IRS-2, leading to degradation of IRS-2. This dissociation was prevented by treatment with LY294002, a PI3K inhibitor, indicating that insulin activation of the PI3K pathway leads to dissociation of IRS-2 from USP7 and IRS-2 degradation. We obtained similar results for IRS-1 in cells treated with insulin and for IRS-2 in cells treated with IGF-I. Taken together, this is the first report demonstrating that USP7 is an IRS-1/2 deubiquitinating enzyme forming a negative feedback loop in insulin/IGF signaling., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
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10. Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 associates with insulin receptor substrate-1 and enhances insulin actions and adipogenesis.
- Author
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Nakatsu Y, Sakoda H, Kushiyama A, Zhang J, Ono H, Fujishiro M, Kikuchi T, Fukushima T, Yoneda M, Ohno H, Horike N, Kanna M, Tsuchiya Y, Kamata H, Nishimura F, Isobe T, Ogihara T, Katagiri H, Oka Y, Takahashi S, Kurihara H, Uchida T, and Asano T
- Subjects
- Animals, Glucose Intolerance genetics, Glucose Intolerance metabolism, Hep G2 Cells, Humans, Insulin Receptor Substrate Proteins genetics, Mice, Mice, Knockout, Mice, Obese, NIMA-Interacting Peptidylprolyl Isomerase, Peptidylprolyl Isomerase genetics, Phosphatidylinositol 3-Kinases genetics, Phosphatidylinositol 3-Kinases metabolism, Phosphorylation physiology, Protein Binding physiology, Protein Structure, Tertiary, Proto-Oncogene Proteins c-akt genetics, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction physiology, Adipogenesis physiology, Insulin metabolism, Insulin Receptor Substrate Proteins metabolism, Liver metabolism, Peptidylprolyl Isomerase metabolism
- Abstract
Peptidyl-prolyl cis/trans isomerase NIMA-interacting 1 (Pin1) is a unique enzyme that associates with the pSer/Thr-Pro motif and catalyzes cis-trans isomerization. We identified Pin1 in the immunoprecipitates of overexpressed IRS-1 with myc and FLAG tags in mouse livers and confirmed the association between IRS-1 and Pin1 by not only overexpression experiments but also endogenously in the mouse liver. The analysis using deletion- and point-mutated Pin1 and IRS-1 constructs revealed the WW domain located in the N terminus of Pin1 and Ser-434 in the SAIN (Shc and IRS-1 NPXY binding) domain of IRS-1 to be involved in their association. Subsequently, we investigated the role of Pin1 in IRS-1 mediation of insulin signaling. The overexpression of Pin1 in HepG2 cells markedly enhanced insulin-induced IRS-1 phosphorylation and its downstream events: phosphatidylinositol 3-kinase binding with IRS-1 and Akt phosphorylation. In contrast, the treatment of HepG2 cells with Pin1 siRNA or the Pin1 inhibitor Juglone suppressed these events. In good agreement with these in vitro data, Pin1 knock-out mice exhibited impaired insulin signaling with glucose intolerance, whereas adenoviral gene transfer of Pin1 into the ob/ob mouse liver mostly normalized insulin signaling and restored glucose tolerance. In addition, it was also demonstrated that Pin1 plays a critical role in adipose differentiation, making Pin1 knock-out mice resistant to diet-induced obesity. Importantly, Pin1 expression was shown to be up-regulated in accordance with nutrient conditions such as food intake or a high-fat diet. Taken together, these observations indicate that Pin1 binds to IRS-1 and thereby markedly enhances insulin action, essential for adipogenesis.
- Published
- 2011
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11. Insulin receptor substrates form high-molecular-mass complexes that modulate their availability to insulin/insulin-like growth factor-I receptor tyrosine kinases.
- Author
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Fukushima T, Arai T, Ariga-Nedachi M, Okajima H, Ooi Y, Iijima Y, Sone M, Cho Y, Ando Y, Kasahara K, Ozoe A, Yoshihara H, Chida K, Okada S, Kopchick JJ, Asano T, Hakuno F, and Takahashi S
- Subjects
- 3T3-L1 Cells, Animals, Bucladesine metabolism, HEK293 Cells, Humans, Mice, Phosphorylation, Thyroid Gland cytology, Thyroid Gland metabolism, Tumor Necrosis Factor-alpha pharmacology, Tyrosine metabolism, Adipocytes metabolism, Insulin metabolism, Insulin Receptor Substrate Proteins metabolism, Multiprotein Complexes metabolism, Receptor, IGF Type 1 metabolism
- Abstract
Insulin receptor substrates (IRSs) are phosphorylated by activated insulin/insulin-like growth factor (IGF)-I receptor tyrosine kinases. Phosphotyrosyl IRSs are recognized by signaling molecules possessing src homology region 2 (SH2) domains, which mediate various insulin/IGF bioactivities. However, we have shown that IRSs are also associated with other proteins by a phosphotyrosine-independent mechanism. Here, we demonstrated that IRSs form high-molecular-mass complexes (we named these complexes IRSomes) with various proteins and we elucidated their possible roles. Blue native-polyacrylamide gel electrophoresis of cell lysates revealed IRSome formation. Some proteins associated with IRSs in IRS-isoform-, cell-type-, or stimulus-specific manners. Results of the in vitro tyrosine phosphorylation assay indicated that tyrosine phosphorylation of IRS-1 by insulin receptor was decreased when IRS-1 was contained in IRSomes prepared from 3T3-L1 adipocytes treated with TNF-α. Also, tyrosine phosphorylation of IRS-2 by IGF-I receptor was increased when IRS-2 was contained in IRSomes prepared from FRTL-5 thyrocytes treated with dibutyryl cAMP. These results demonstrated that cytokine/hormone-induced formation of IRSomes modulates availability of IRSs to receptor tyrosine kinases., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
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12. Dietary protein deprivation upregulates insulin signaling and inhibits gluconeogenesis in rat liver.
- Author
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Toyoshima Y, Tokita R, Ohne Y, Hakuno F, Noguchi T, Minami S, Kato H, and Takahashi S
- Subjects
- Animals, Blood Glucose metabolism, Dietary Proteins pharmacology, Energy Metabolism, Homeostasis physiology, Insulin blood, Insulin pharmacology, Insulin Receptor Substrate Proteins metabolism, Liver drug effects, Liver enzymology, Male, Phosphatidylinositol 3-Kinase metabolism, Phosphorylation, Protein Deficiency metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Rats, Wistar, Receptor, Insulin genetics, Receptor, Insulin metabolism, Signal Transduction drug effects, TOR Serine-Threonine Kinases metabolism, Up-Regulation, Diet, Protein-Restricted adverse effects, Dietary Proteins administration & dosage, Gluconeogenesis, Insulin metabolism, Liver metabolism
- Abstract
This study was undertaken to elucidate the effects of dietary protein deprivation on glucose metabolism and hepatic insulin signaling in rats. The results of glucose and pyruvate tolerance tests in rats fed with a 12% casein diet (12C) and a protein-free diet (PF) indicated that protein deprivation enhanced clearance of blood glucose and suppressed gluconeogenesis. Correspondingly, the mRNA level of hepatic phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme, was suppressed by dietary protein deprivation. In PF-fed rats, total tyrosine phosphorylation of insulin receptor (IR) in the liver induced by insulin injection was enhanced compared with 12C pair-fed rats due to an increase in IR protein level. In addition, protein deprivation caused an increase in protein levels of IR substrate 1 (IRS1) and IRS2, leading to the marked enhancement of insulin-induced tyrosine phosphorylation of IRS2 and its binding to the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K). Based on these results, we conclude that protein deprivation suppresses gluconeogenesis by a mechanism primarily mediated by the enhancement of the insulin signals through the IR/IRS/PI3K/mammalian target of rapamycin complex 1 pathway in the liver. Taken together with our previous report, these findings suggest that tissue-specific potentiation of insulin action in the liver and the skeletal muscle plays important roles in maintaining glucose homeostasis even when energy usage is reduced by dietary protein deprivation.
- Published
- 2010
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13. 53BP2S, interacting with insulin receptor substrates, modulates insulin signaling.
- Author
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Hakuno F, Kurihara S, Watson RT, Pessin JE, and Takahashi S
- Subjects
- Adaptor Proteins, Signal Transducing metabolism, Alternative Splicing, Animals, Apoptosis, CHO Cells, COS Cells, Chlorocebus aethiops, Cricetinae, Cricetulus, Insulin Receptor Substrate Proteins, Intracellular Signaling Peptides and Proteins metabolism, Mice, Phosphoproteins metabolism, Proto-Oncogene Proteins c-akt metabolism, src Homology Domains, Carrier Proteins physiology, Insulin metabolism, Intracellular Signaling Peptides and Proteins physiology, Signal Transduction
- Abstract
It is well known that insulin receptor substrates (IRS) act as a mediator for signal transduction of insulin, insulin-like growth factors, and several cytokines. To identify proteins that interact with IRS and modulate IRS-mediated signals, we performed yeast two-hybrid screening with IRS-1 as bait. Out of 109 cDNA-positive clones identified from a human placental cDNA library, two clones encoded 53BP2, p53-binding protein 2 (53BP2S), a short form splicing variant of the apoptosis-stimulating protein of p53 that possesses Src homology region 3 domain, and ankyrin repeats domain, and had been reported to interact with p53, Bcl-2, and NF-kappaB. Interaction of 53BP2S with IRS-1 was confirmed by glutathione S-transferase pull-down and co-immunoprecipitation assays in COS-7 cells and 3T3-L1 adipocytes. The Src homology region 3 domain and ankyrin repeats domain of 53BP2S were responsible for its interaction with IRS-1, whereas the phosphotyrosine binding domain and a central domain (amino acid residues 750-861) of IRS-1 were required for its interaction with 53BP2S. In CHO-C400 cells, expression of 53BP2S reduced insulin-stimulated IRS-1 tyrosine phosphorylation with a concomitant enhancement of IRS-2 tyrosine phosphorylation. In addition, the amount of the phosphatidylinositol 3-kinase regulatory p85 subunit associated with tyrosine-phosphorylated proteins, and activation of Akt was enhanced by 53BP2S expression. Although 53BP2S also enhanced Akt activation in 3T3-L1 adipocytes, insulin-induced glucose transporter 4 translocation was markedly inhibited in accordance with reduction of insulin-induced AS160 phosphorylation. Together these data demonstrate that 53BP2S interacts and modulates the insulin signals mediated by IRSs.
- Published
- 2007
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14. Insulin/insulin-like growth factor-like activity in the aqueous extracts of the rotifer Brachionus plicatilis
- Author
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Ozaki, Yori, Kaneko, Gen, Hakuno, Fumihiko, Takahashi, Shin-Ichiro, and Watabe, Shugo
- Published
- 2013
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15. IGF research 2016–2018.
- Author
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Takahashi, Shin-Ichiro
- Abstract
This article is based on my presentation at the 9th International Congress of the Growth Hormone Research and Insulin-like Growth Factor (IGF) Societies at Seattle, USA on 17th, September 2018. In the article, after a general introduction to IGF research, I briefly review the IGF research being published from 2016 to 2018, focusing on what I believe represent the most interesting areas of progress. These areas include ligands of the IGF-I receptor, ligand binding to the IGF-I receptor, long-term signaling through the IGF-I receptor, intracellular organelles where IGF signals are transmitted, and novel functions of the IGFBPs. Lastly, I discuss future directions of IGF research from my point of view. • Viral insulin-like peptides are members of the insulin/IGF superfamily. • Structural biology research showed how ligands bind to the IGF-I receptor. • IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling. • IGF signaling is essential for mitochondrial biogenesis and mitophagy in cancer cells. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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16. The novel functions of high-molecular-mass complexes containing insulin receptor substrates in mediation and modulation of insulin-like activities: emerging concept of diverse functions by IRS-associated proteins.
- Author
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Hakuno, Fumihiko, Fukushima, Toshiaki, Yoneyama, Yosuke, Kamei, Hiroyasu, Ozoe, Atsufumi, Yoshihara, Hidehito, Yamanaka, Daisuke, Shibano, Takashi, Sone-Yonezawa, Meri, Bu-Chin Yu, Chida, Kazuhiro, and Takahashi, Shin-Ichiro
- Subjects
SOMATOMEDIN ,INSULIN receptors ,BIOTRANSFORMATION (Metabolism) - Abstract
Insulin-like peptides, such as insulin-like growth factors (IGFs) and insulin, induce a variety of bioactivities, such as growth, differentiation, survival, increased anabolism, and decreased catabolism in many cell types and in vivo. In general, IGFs or insulin bind to IGF-I receptor (IGF-IR) or insulin receptor (IR), activating the receptor tyrosine kinase. Insulin receptor substrates (IRSs) are known to be major substrates of receptor kinases, mediating IGF/insulin signals to direct bioactivities. Recently, we discovered that IRSs form high-molecular-mass complexes (referred to here as IRSomes) even without IGF/insulin stimulation. These complexes contain proteins (referred to here as IRSAPs; IRS-associated proteins), which modulate tyrosine phosphorylation of IRSs by receptor kinases, control IRS stability, and determine intracellular localization of IRSs. In addition, in these complexes, we found not only proteins that are involved in RNA metabolism but also RNAs themselves. Thus, IRSAPs possibly contribute to modulation of IGF/insulin bioactivities. Since it is established that disorder of modulation of insulin-like activities causes various age-related diseases including cancer, we could propose that the IRSome is an important target for treatment of these diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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17. Novel repressor regulates insulin sensitivity through interaction with Foxo1.
- Author
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Nakae, Jun, Cao, Yongheng, Hakuno, Fumihiko, Takemori, Hiroshi, Kawano, Yoshinaga, Sekioka, Risa, Abe, Takaya, Kiyonari, Hiroshi, Tanaka, Toshiya, Sakai, Juro, Takahashi, Shin-Ichiro, and Itoh, Hiroshi
- Subjects
GENETIC repressors ,INSULIN ,FORKHEAD transcription factors ,GLUCOSE metabolism ,ADIPOSE tissues ,ACETYLATION ,LABORATORY mice - Abstract
Forkhead box-containing protein o (Foxo) 1 is a key transcription factor in insulin and glucose metabolism. We identified a Foxo1-CoRepressor (FCoR) protein in mouse adipose tissue that inhibits Foxo1's activity by enhancing acetylation via impairment of the interaction between Foxo1 and the deacetylase Sirt1 and via direct acetylation. FCoR is phosphorylated at Threonine 93 by catalytic subunit of protein kinase A and is translocated into nucleus, making it possible to bind to Foxo1 in both cytosol and nucleus. Knockdown of FCoR in 3T3-F442A cells enhanced expression of Foxo target and inhibited adipocyte differentiation. Overexpression of FCoR in white adipose tissue decreased expression of Foxo-target genes and adipocyte size and increased insulin sensitivity in Lepr
db/db mice and in mice fed a high-fat diet. In contrast, Fcor knockout mice were lean, glucose intolerant, and had decreased insulin sensitivity that was accompanied by increased expression levels of Foxo-target genes and enlarged adipocytes. Taken together, these data suggest that FCoR is a novel repressor that regulates insulin sensitivity and energy metabolism in adipose tissue by acting to fine-tune Foxo1 activity. [ABSTRACT FROM AUTHOR]- Published
- 2012
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18. Familial short stature is associated with a novel dominant-negative heterozygous insulin-like growth factor 1 receptor ( IGF1 R) mutation.
- Author
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Kawashima, Yuki, Hakuno, Fumihiko, Okada, Shin‐ichi, Hotsubo, Tomoyuki, Kinoshita, Tomoe, Fujimoto, Masanobu, Nishimura, Rei, Fukushima, Toshiaki, Hanaki, Keiichi, Takahashi, Shin‐Ichiro, and Kanzaki, Susumu
- Subjects
INSULIN ,GROWTH factors ,GENETIC mutation - Abstract
A letter to the editor is presented in response to the article related to familial short stature related to novel dominant-negative heterozygous insulin-including growth factor 1 receptor ( IGF1 R) mutation.
- Published
- 2014
- Full Text
- View/download PDF
19. Identification of Bombyx mori Akt and its phosphorylation by bombyxin stimulation
- Author
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Nagata, Shinji, Hakuno, Fumihiko, Takahashi, Shin-Ichiro, and Nagasawa, Hiromichi
- Subjects
- *
SILKWORMS , *PHOSPHORYLATION , *PROTEIN kinases , *INSULIN , *HOMOLOGY (Biology) , *MOLECULAR cloning , *POLYMERASE chain reaction - Abstract
Abstract: Akt, a Ser/Thr protein kinase involved in insulin signaling, was identified from the silkworm, Bombyx mori. Bombyx Akt (BomAkt) is composed of 493 amino acid residues including regions conserved in other Akts: the Pleckstrin homology and kinase domains, and a dual phosphorylation site essential for kinase activation. Commercially available antibodies against mammalian Akt and phosphoAkt were able to recognize BomAkt and phosphorylated BomAkt in HEK293 cells expressing BomAkt. Additionally, phosphorylation of BomAkt was detectable in insulin-like growth factor (IGF)-I stimulated-HEK293 cells expressing BomAkt. RT-PCR and immunoblotting analyses revealed that BomAkt is expressed ubiquitously in Bombyx larvae. Phosphorylation of BomAkt was observed both in the isolated fat body after exposure to bombyxin, an endogenous insulin-like peptide, and in the larval fat body by refeeding a diet after starvation. These results suggest that dietary intake may activate the insulin signaling pathway, including Akt, through bombyxin action in B. mori. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
20. Insulin receptor substrate-3, interacting with Bcl-3, enhances p50 NF-{kappa}B activity
- Author
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Takahashi, Shin-Ichiro [Departments of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo 113-8657 (Japan)]
- Published
- 2010
- Full Text
- View/download PDF
21. Differential subcellular localization of insulin receptor substrates depends on C-terminal regions and importin {beta}
- Author
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Takahashi, Shin-Ichiro [Laboratory of Cell Regulation, Departments of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 (Japan)]
- Published
- 2008
- Full Text
- View/download PDF
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