1. Long term culture of rat soleus muscle in vitro. Its effects on glucose utilization and insulin sensitivity.
- Author
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Stace PB, Marchington DR, Kerbey AL, and Randle PJ
- Subjects
- Adenosine Deaminase pharmacology, Adenosine Triphosphate metabolism, Animals, Glycogen metabolism, L-Lactate Dehydrogenase metabolism, Muscles cytology, Muscles drug effects, Organ Culture Techniques methods, Phosphocreatine metabolism, Rats, Reference Values, Glucose metabolism, Insulin pharmacology, Muscles metabolism
- Abstract
Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K+); increased basal glucose utilization up to two-fold; had no effect on the maximum response to insulin; and had no effect on sensitivity to insulin except in the presence of adenosine deaminase. Thus in vitro neither denervation nor absence of insulin had any marked effects in 24 h to decrease responses to insulin.
- Published
- 1990
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