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Extensive application of routine insecticide i.e., dichlorodiphenyltrichloroethane (DDT) to control Phlebotomus argentipes (Diptera: Psychodidae), the proven vector of visceral leishmaniasis in India, had evoked the problem of resistance/tolerance against DDT, eventually nullifying the DDT dependent strategies to control this vector. Because tolerating an hour-long exposure to DDT is not challenging enough for the resistant P. argentipes, estimating susceptibility by exposing sand flies to insecticide for just an hour becomes a trivial and futile task.Therefore, this bioassay study was carried out to investigate the maximum limit of exposure time to which DDT resistant P. argentipes can endure the effect of DDT for their survival. The mortality rate of laboratory-reared DDT resistant strain P. argentipes exposed to DDT was studied at discriminating time intervals of 60 min and it was concluded that highly resistant sand flies could withstand up to 420 min of exposure to this insecticide. Additionally, the lethal time for female P. argentipes was observed to be higher than for males suggesting that they are highly resistant to DDT's toxicity. Our results support the monitoring of tolerance limit with respect to time and hence points towards an urgent need to change the World Health Organization's protocol for susceptibility identification in resistant P. argentipes.

Despite its epidemiological importance, the impact of insecticide resistance on vector-parasite interactions and malaria transmission is poorly understood. Here, we explored the impact of Plasmodium infection on the level of insecticide resistance to dichlorodiphenyltrichloroethane (DDT) in field-caught Anopheles gambiae sensu stricto homozygous for the kdr mutation. Results showed that kdr homozygous mosquitoes that fed on infectious blood were more susceptible to DDT than mosquitoes that fed on noninfectious blood during both ookinete development (day 1 after the blood meal) and oocyst maturation (day 7 after the blood meal) but not during sporozoite invasion of the salivary glands. Plasmodium falciparum infection seemed to impose a fitness cost on mosquitoes by reducing the ability of kdr homozygous A. gambiae sensu stricto to survive exposure to DDT. These results suggest an interaction between Plasmodium infection and the insecticide susceptibility of mosquitoes carrying insecticide-resistant alleles. We discuss this finding in relation to vector control efficacy., (© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)

Currently, there is a strong trend towards increasing insecticide-based vector control coverage in malaria endemic countries. The ecological consequence of insecticide applications has been mainly studied regarding the selection of resistance mechanisms; however, little is known about their impact on vector competence in mosquitoes responsible for malaria transmission. As they have limited toxicity to mosquitoes owing to the selection of resistance mechanisms, insecticides may also interact with pathogens developing in mosquitoes. In this study, we explored the impact of insecticide exposure on Plasmodium falciparum development in insecticide-resistant colonies of Anopheles gambiae s.s., homozygous for the ace-1 G119S mutation (Acerkis) or the kdr L1014F mutation (Kdrkis). Exposure to bendiocarb insecticide reduced the prevalence and intensity of P. falciparum oocysts developing in the infected midgut of the Acerkis strain, whereas exposure to dichlorodiphenyltrichloroethane reduced only the prevalence of P. falciparum infection in the Kdrkis strain. Thus, insecticide resistance leads to a selective pressure of insecticides on Plasmodium parasites, providing, to our knowledge, the first evidence of genotype by environment interactions on vector competence in a natural Anopheles-Plasmodium combination. Insecticide applications would affect the transmission of malaria in spite of resistance and would reduce to some degree the impact of insecticide resistance on malaria control interventions., (© 2014 The Author(s) Published by the Royal Society. All rights reserved.)

The objective of this study was to identify the Hsp70s in Paracoccus marginatus and explore their roles in P. marginatus's resistance to temperature and insecticide stress. The full-length cDNA sequences of PmHsp70s were obtained by PCR cloning and sequencing. The physicochemical and structural characteristics of PmHsp70s were analyzed, and a phylogenetic tree was constructed. The gene expressions of PmHsp70s were detected using qRT-PCR to explore the impacts of temperature and insecticide stress on P. marginatus. A total of 12 PmHsp70s were identified and cloned. The amino acids encoded by PmHsp70s were found to contain highly conserved regions characteristic of the Hsp70 family. The subcellular localization results showed that the majority of PmHsp70s were located in the cytoplasm. A total of 13 unique conserved motifs were identified for the PmHsp70s, of which 9 were shared motifs. The phylogenetic tree showed that the 12 PmHsp70s could be clustered into five branches, with the closest evolutionary relationship observed with the Phenacoccus solenopsis. The expression of the majority of PmHsp70s was up-regulated in P. marginatus when subjected to heat stress, with the higher expression fold change observed for PmHsp70-9, PmHsp70-11, and PmHsp70-12. The expression of specific PmHsp70s was notably suppressed under cold stress, whereas the expression of others was markedly enhanced. Upon exposure to chlorfenapyr and lambda-cyhalothrin, the expressions of PmHsp70-11 and PmHsp70-12 were significantly up-regulated with the highest expression fold change, respectively. The results revealed the significance of specific PmHsp70s in the resistance of P. marginatus to temperature and insecticide stress. This study improved our understanding of the mechanisms underlying P. marginatus's adaptive responses to unfavorable environmental conditions. [ABSTRACT FROM AUTHOR]

Simple Summary: Insects transmit a wide variety of pathogens, including parasites, bacteria, and viruses, to human and economically important crops. Since pathogen transmission threatens public health and economic activities, insecticides are the main strategy to control insect populations. The continued use of insecticides has led to resistant populations where chemicals are no longer effective. It is unknown if insecticide resistance (IR) could impact insects' other characteristics, such as their ability to infect, maintain infection, or transmit pathogens, a trait known as vector competence (VC). In this review, we analyze the literature that involves the study of VC and IR or insecticide exposure in three main approaches; studies conducted in the field versus laboratory-designed experiments, the impact of insecticide exposure on pathogen transmission, and studies performed on vectors of crop pathogens. The evidence points out three different patterns where enhancement, impairment, or neutral effects are seen between IR and VC. It is of great concern that IR could enhance VC since it increases the risk of epidemics. More detailed and standardized studies are needed to confirm this relationship. Finally, results from this investigation could help create evidence-based vector control programs. The primary strategy to avoid adverse impacts from insect-mediated pathogen transmission is the chemical control of vector populations through insecticides; its continued use has led to insecticide resistance and unknown consequences on vector competence. This review aims to systematically analyze and synthesize the research on the influence of insecticide resistance (IR) on vector competence (VC). Thirty studies met the inclusion criteria. Twenty studies, conducted either in laboratory or field settings, described the influence of phenotypic insecticide resistance and mechanisms on VC in vectors of human pathogens. Seven studies showed the effect of exposure to insecticides on VC in vectors of human pathogens. Three studies reported the influence of phenotypic resistance and mechanisms on VC in crop pests. The evidence shows that IR could enhance, impair, or have no direct effect on VC in either field or laboratory-designed studies. Similar positive and negative trends are found in pest vectors in crops and studies of insecticide exposure and VC. Even though there is evidence that exposure to insecticides and IR can enhance VC, thus increasing the risk of pathogen transmission, more investigations are needed to confirm the observed patterns and what implications these factors could have in vector control programs. [ABSTRACT FROM AUTHOR]

For many decades, insecticides have been used to control mosquito populations in their larval and adult stages. Although changes in the population genetics, physiology, and behavior of mosquitoes exposed to lethal and sublethal doses of insecticides are expected, the relationships between these changes and their abilities to transmit pathogens remain unclear. Thus, we conducted a comprehensive review on the sublethal effects of insecticides and their contributions to insecticide resistance in mosquitoes, with the main focus on pyrethroids. We discuss the direct and acute effects of sublethal concentrations on individuals and populations, the changes in population genetics caused by the selection for resistance after insecticide exposure, and the major mechanisms underlying such resistance. Sublethal exposures negatively impact the individual’s performance by affecting their physiology and behavior and leaving them at a disadvantage when compared to unexposed organisms. How these sublethal effects could change mosquito population sizes and diversity so that pathogen transmission risks can be affected is less clear. Furthermore, despite the beneficial and acute aspects of lethality, exposure to higher insecticide concentrations clearly impacts the population genetics by selecting resistant individuals, which may bring further and complex interactions for mosquitoes, vertebrate hosts, and pathogens. Finally, we raise several hypotheses concerning how the here revised impacts of insecticides on mosquitoes could interplay with vector-mediated pathogens’ transmission.

Simple Summary: Mosquitoes are one of the greatest threats to human lives; they transmit a wide range of pathogens, including viruses that cause lethal diseases. Mosquitoes are found in both aquatic (as larvae or pupae) and terrestrial (as adults) environments during their complex life cycle. For decades, insecticides have been systematically used on mosquitoes with the aim to reduce their population. Little is known about how the stress resulting from the exposure of mosquitoes to insecticides impacts the tri-partite relationship between the mosquitoes, their vertebrate hosts, and the pathogens they transmit. In this work, we review existing experimental evidence to obtain a broad picture on the potential effects of the (sub)lethal exposure of hematophagous mosquitoes to different insecticides. We have focused on studies that have advanced our understanding of their physiological and behavioral responses (including the mechanisms behind insecticide resistance) and the spread of pathogens by these vectors—understudied but critically important issues for epidemiology. Studying these exposure-related effects is of paramount importance for predicting how they respond to insecticide exposure and whether this exposure makes them more or less likely to transmit pathogens. For many decades, insecticides have been used to control mosquito populations in their larval and adult stages. Although changes in the population genetics, physiology, and behavior of mosquitoes exposed to lethal and sublethal doses of insecticides are expected, the relationships between these changes and their abilities to transmit pathogens remain unclear. Thus, we conducted a comprehensive review on the sublethal effects of insecticides and their contributions to insecticide resistance in mosquitoes, with the main focus on pyrethroids. We discuss the direct and acute effects of sublethal concentrations on individuals and populations, the changes in population genetics caused by the selection for resistance after insecticide exposure, and the major mechanisms underlying such resistance. Sublethal exposures negatively impact the individual's performance by affecting their physiology and behavior and leaving them at a disadvantage when compared to unexposed organisms. How these sublethal effects could change mosquito population sizes and diversity so that pathogen transmission risks can be affected is less clear. Furthermore, despite the beneficial and acute aspects of lethality, exposure to higher insecticide concentrations clearly impacts the population genetics by selecting resistant individuals, which may bring further and complex interactions for mosquitoes, vertebrate hosts, and pathogens. Finally, we raise several hypotheses concerning how the here revised impacts of insecticides on mosquitoes could interplay with vector-mediated pathogens' transmission. [ABSTRACT FROM AUTHOR]

Currently, there is a strong trend towards increasing insecticide-based vector control coverage in malaria endemic countries. The ecological consequence of insecticide applications has been mainly studied regarding the selection of resistance mechanisms; however, little is known about their impact on vector competence in mosquitoes responsible for malaria transmission. As they have limited toxicity to mosquitoes owing to the selection of resistance mechanisms, insecticides may also interact with pathogens developing in mosquitoes. In this study, we explored the impact of insecticide exposure on Plasmodium falciparum development in insecticide-resistant colonies of Anopheles gambiae s.s., homozygous for the ace-1 G119S mutation (Acerkis) or the kdr L1014F mutation (Kdrkis). Exposure to bendiocarb insecticide reduced the prevalence and intensity of P. falciparum oocysts developing in the infected midgut of the Acerkis strain, whereas exposure to dichlorodiphenyltrichloroethane reduced only the prevalence of P. falciparum infection in the Kdrkis strain. Thus, insecticide resistance leads to a selective pressure of insecticides on Plasmodium parasites, providing, to our knowledge, the first evidence of genotype by environment interactions on vector competence in a natural Anopheles-Plasmodium combination. Insecticide applications would affect the transmission of malaria in spite of resistance and would reduce to some degree the impact of insecticide resistance on malaria control interventions. [ABSTRACT FROM AUTHOR]