1. Production of human c-myc protein in insect cells infected with a baculovirus expression vector.
- Author
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Miyamoto C, Smith GE, Farrell-Towt J, Chizzonite R, Summers MD, and Ju G
- Subjects
- Animals, Cells, Cultured, Humans, Molecular Weight, Moths, Occlusion Body Matrix Proteins, Promoter Regions, Genetic, Protein Processing, Post-Translational, Proto-Oncogene Proteins biosynthesis, Recombinant Proteins biosynthesis, Viral Proteins genetics, Viral Structural Proteins, Genetic Vectors, Insect Viruses genetics, Proto-Oncogene Proteins genetics, Recombinant Proteins genetics
- Abstract
A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. By immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. The insect-derived proteins were compared with recombinant human c-myc-encoded proteins synthesized in Escherichia coli and Saccharomyces cerevisiae cells. The c-myc gene product was found predominantly in the nucleus by subcellular fractionation of infected insect cells.
- Published
- 1985
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