Objective To understand the health status of female medical staffs in a class 3A hospital of Chongqing City, and to focus on analyzing the prevalence of gynecological diseases and related influencing factors in order to provide the reference information and decision-making basis for relevant units to formulate the health maintenance measures for female medical staffs.Methods The physical examination data of 1 912 female medical staffs participating in the physical examination in a class 3A hospital of Chongqing City during 2021 were classified and sorted, and the influence of the work post, age and education level of the medical staffs on the prevalence of gynecological diseases was analyzed. Results Among 1 912 female medical staffs, the prevalence rate of gynecological diseases was 92.1%（1 761/1 912）.The top five were mammary hyperplasia（87.4%,1 672/1 912）,cervical diseases（38.1%,729/1 912）,breast cysts（9.6%,184/1 912）,uterine fibroids（8.6%,165/1 912） and ovarian cysts（0.6%,11/1 912）.The non-clinical posts, age increase and lower education background were the risk factors of breast hyperplasia.The age increase is a risk factor for cervical diseases. Conclusion The incidence rate of gynecological diseases among female medical staffs is high, which should be paid attention to, and various measures should be taken to promote the health of female medical staff. [ABSTRACT FROM AUTHOR]