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Objective: To investigate the changes of platelet diffusion function during storage of apheresis platelets from blood donors. Methods: A total of 66 blood donors, who participated in platelet apheresis blood donation at blood centers in the region from August 2017 to June 2020, were selected as the research subjects. The platelet apheresis blood sample of each case was divided into 3 tubes-A tube, B tube and C tube, which were stored in a platelet incubator at 20 ℃, tube A 1d after storage, tube B 3 d after storage, and tube C 5 d after storage were detected platelet diffusion functional changes. Results: There were not significantly differences in the Hct (Hematocrit), Hb (Hemoglobin), PDW (Platelet distribution width), WBC (leukocyte), RBC (Red blood cell), Platelets(PLT), and MPV (Mean platelet volume) among tube A, tube B and tube C(P>0.05). The P-LCR (platelet-larger cell ratio), serum interleukin (IL)-1β, IL-6, and tumor necrosis factor (Tumor necrosis factor, TNF)-α content of tube B and tube C were higher than those of tube A (P<0.05), tube C were higher than tube B (P<0.05). The expression levels of platelet membrane glycoprotein CD62p in tube B and tube C were higher than those in tube A (P<0.05), and the maximum platelet aggregation rate in tube B and tube C was lower than that in tube A (P<0.05), and the difference between tube C and tube B was also statistically significant (P<0.05). The platelet pass rate after diffusion of tube A, tube B and tube C were 97.0 %, 86.4 %, and 77.3 %, respectively, with statistically significant differences among the three groups (P<0.05). Conclusion: The storage time of apheresis platelets donated by blood donors may affect the platelet diffusion function, increase the release of inflammatory cytokines, can reduce the maximum platelet aggregation rate and increase the expression level of platelet mem- brane glycoprotein CD62p, thus reducing the efficacy of infusion. [ABSTRACT FROM AUTHOR]