1. PKCλ/ι regulates Th17 differentiation and house dust mite-induced allergic airway inflammation.
- Author
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Yang Y, Dong P, Zhao J, Zhou W, Zhou Y, Xu Y, Mei C, Guo F, Zheng Y, and Yang JQ
- Subjects
- Animals, Dermatophagoides pteronyssinus immunology, Embryo, Mammalian, Female, Isoenzymes genetics, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Pregnancy, Protein Kinase C genetics, Cell Differentiation genetics, Inflammation genetics, Inflammation immunology, Isoenzymes physiology, Protein Kinase C physiology, Pyroglyphidae immunology, Respiratory Hypersensitivity genetics, Respiratory Hypersensitivity immunology, Th17 Cells physiology
- Abstract
Asthma is a chronic airway inflammation in which Th2 and Th17 cells play critical roles in its pathogenesis. We have reported that atypical protein kinase (PKC) λ/ι is a new regulator for Th2 differentiation and function. However, the role of PKCλ/ι for Th17 cells remains elusive. In this study, we explored the effect of PKCλ/ι on Th17 cells in the context of ex vivo cell culture systems and an in vivo murine model of allergic airway inflammation with the use of activated T cell-specific conditional PKCλ/ι-deficient mice. Our findings indicate that PKCλ/ι regulates Th17 cells. The secretion of Th17 effector cytokines, including IL-17, IL-21 and IL-22, were inhibited from PKCλ/ι-deficient T cells under non-skewing or Th17-skewing culture conditions. Moreover, the impaired Th17 differentiation and function by the PKCλ/ι-deficiency was associated with the downregulation of Stat3 and Rorγt, key Th17 transcription factors. We developed a model of Th17 and neutrophil-involved allergic airway inflammation by intratracheal inoculation of house dust mites. PKCλ/ι-deficiency significantly inhibited airway inflammations. The infiltrating cells in the lungs and bronchoalveolar lavage fluids were significantly reduced in conditional PKCλ/ι-deficient mice. Th17 effector cytokines were reduced in the bronchoalveolar lavage fluids and lungs at protein and mRNA levels. Thus, PKCλ/ι emerges as a critical regulator of Th17 differentiation and allergic airway hyperresponsiveness., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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