1. Acute skin exposure to ultraviolet light triggers neutrophil-mediated kidney inflammation.
- Author
-
Skopelja-Gardner S, Tai J, Sun X, Tanaka L, Kuchenbecker JA, Snyder JM, Kubes P, Mustelin T, and Elkon KB
- Subjects
- Animals, Calgranulin B genetics, Cell Movement radiation effects, Disease Models, Animal, Gene Expression Regulation radiation effects, Humans, Inflammation etiology, Inflammation pathology, Interleukin-17 genetics, Kidney injuries, Kidney pathology, Kidney radiation effects, Lipocalin-2 genetics, Mice, Neutrophils metabolism, Neutrophils pathology, Skin injuries, Ultraviolet Rays adverse effects, Vascular Cell Adhesion Molecule-1 genetics, Inflammation blood, Kidney metabolism, Neutrophils radiation effects, Skin radiation effects
- Abstract
Photosensitivity to ultraviolet (UV) light affects up to ∼80% of lupus patients. Sunlight exposure can exacerbate local as well as systemic manifestations of lupus, including nephritis, by mechanisms that are poorly understood. Here, we report that acute skin exposure to UV light triggers a neutrophil-dependent injury response in the kidney characterized by upregulated expression of endothelial adhesion molecules as well as inflammatory and injury markers associated with transient proteinuria. We showed that UV light stimulates neutrophil migration not only to the skin but also to the kidney in an IL-17A-dependent manner. Using a photoactivatable lineage tracing approach, we observed that a subset of neutrophils found in the kidney had transited through UV light-exposed skin, suggesting reverse transmigration. Besides being required for the renal induction of genes encoding mediators of inflammation ( vcam-1 , s100A9 , and Il-1b ) and injury ( lipocalin-2 and kim-1 ), neutrophils significantly contributed to the kidney type I interferon signature triggered by UV light. Together, these findings demonstrate that neutrophils mediate subclinical renal inflammation and injury following skin exposure to UV light. Of interest, patients with lupus have subpopulations of blood neutrophils and low-density granulocytes with similar phenotypes to reverse transmigrating neutrophils observed in the mice post-UV exposure, suggesting that these cells could have transmigrated from inflamed tissue, such as the skin., Competing Interests: The authors declare no competing interest.
- Published
- 2021
- Full Text
- View/download PDF