1. Long noncoding RNA MEG3 expressed in human dental pulp regulates LPS-Induced inflammation and odontogenic differentiation in pulpitis.
- Author
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Liu M, Chen L, Wu J, Lin Z, and Huang S
- Subjects
- Adolescent, Adult, Apoptosis, Biomarkers metabolism, Cell Proliferation, Cells, Cultured, Cytokines metabolism, Dental Pulp metabolism, Gene Expression Regulation, Humans, Inflammation chemically induced, Inflammation genetics, Inflammation metabolism, Pulpitis genetics, Pulpitis metabolism, Young Adult, Cell Differentiation, Dental Pulp cytology, Inflammation pathology, Lipopolysaccharides adverse effects, Odontogenesis, Pulpitis pathology, RNA, Long Noncoding genetics
- Abstract
Pulpitis refers to inflammation of the inner pulp by invading microbes, and tissue repair occurs due to odontogenic differentiation of human dental pulp cells (hDPCs) with multidifferentiation potential. Long noncoding RNAs (lncRNAs) can modulate numerous pathological and biological processes; however, the role of lncRNAs in the inflammation and regeneration of the dentin-pulp complex in pulpitis is unclear. Here, we performed high-throughput sequencing to identify differentially expressed lncRNAs between human normal and inflamed pulp and concluded that lncMEG3 (lncRNA maternally expressed gene 3, MEG3) was significantly upregulated in both inflamed pulp and LPS-treated hDPCs. MEG3 expression in the pulp tissue was detected using the RNAscope® technique. RNA pulldown assays identified the MEG3-interacting proteins and the potential mechanisms. With MEG3 knockdown, we investigated the role of MEG3 in the secretion of inflammatory cytokines in LPS-treated hDPCs and odontogenic differentiation of hDPCs. MEG3 downregulation inhibited the secretion of TNF-α, IL-1β and IL-6 in LPS-treated hDPCs, and the p38/MAPK signaling pathway may be related to this effect. MEG3 knockdown promoted odontogenic differentiation of hDPCs by regulating the Wnt/β-catenin signaling pathway. Our study suggested that MEG3 has a negative effect on inflammation and regeneration of the dentin-pulp complex in pulpitis., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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