1. In Vitro and In Vivo Model for Sepsis Through Non-canonical NLRP3 Inflammasome Activation.
- Author
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Sun X, Kang TB, and Lee KH
- Subjects
- Caspases metabolism, Humans, Interleukin-1beta metabolism, NLR Family, Pyrin Domain-Containing 3 Protein metabolism, Pyroptosis, Inflammasomes metabolism, Sepsis
- Abstract
Sepsis is a complex disorder related to dysregulation of the host response to infection and is a major health problem worldwide owing to its high mortality rates. However, the exact mechanisms causing sepsis remains unclear because of the complexity of the underlying pathways. Dysregulation of non-canonical NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome activation induces septic shock by promoting pyroptosis and pro-inflammatory cytokine production (e.g., interleukin-1β) via caspase-11 and Gasdermin-D. Herein, we describe a rapid, simple method for evaluation of the degree of sepsis by investigating non-canonical inflammasome activation in both in vitro and in vivo models. The method is expected to be useful for testing and screening drugs for the treatment of sepsis., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
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