1. Cobalt phthalocyanine as a biomimetic catalyst in the amperometric quantification of dipyrone using FIA.
- Author
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Boni AC, Wong A, Dutra RA, and Sotomayor Mdel P
- Subjects
- Catalysis, Cytochrome P-450 Enzyme System metabolism, Electrochemistry economics, Flow Injection Analysis economics, Time Factors, Biomimetic Materials chemistry, Dipyrone analysis, Electrochemistry methods, Flow Injection Analysis methods, Indoles chemistry, Organometallic Compounds chemistry
- Abstract
A biomimetic sensor for the determination of dipyrone was prepared by modifying carbon paste with cobalt phthalocyanine (CoPc), and used as an amperometric detector in a flow injection analysis (FIA) system. The results of cyclic voltammetry experiments suggested that CoPc behaved as a biomimetic catalyst in the electrocatalytic oxidation of dipyrone, which involved the transfer of one electron. The optimized FIA procedure employed a flow rate of 1.5 mL min(-1), a 75 μL sample loop, a 0.1 mol L(-1) phosphate buffer carrier solution at pH 7.0 and amperometric detection at a potential of 0.3 V vs. Ag/AgCl. Under these conditions, the proposed method showed a linear response for dipyrone concentrations in the range 5.0 × 10(-6)-6.3 × 10(-3) mol L(-1). Selectivity and interference studies were carried out in order to validate the system for use with pharmaceutical and environmental samples. In addition to being environmentally friendly, the proposed method is a sensitive and selective analytical tool for the determination of dipyrone., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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