1. GENETIC MATCHING OF DATE PALM DEVELOPED UNDER SALINITY STRESS IN VITRO.
- Author
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Alasadi, Mufeed Daher, Abdulwahid, Aqeel Hadi, and Ali, Abdulminam Hussien
- Subjects
DATE palm ,SALINITY ,RAPD technique ,SALT ,ABSCISIC acid ,INDICATORS & test-papers - Abstract
This study was conducted during 2019-2020 on the vegetative shoots of date palm Phoenix dactylifera L. cultivar 'Barhee', which were grown on MS salt media containing sodium chloride (0-25-50-100-150-200 mM) supplemented with 5% sucrose. Genetic variances were assessed using molecular markers RAPD using 8 random primers. The RAPD analysis did not result a polymorphism between the amplified bundles and the absence of genetic differences. The primer (OPE-19 and OPD-10) scored the lowest similarity rate 97%, while the genetic matching was 100% in the other primers. The morphological indicators recorded a significant and gradual decrease in the growth indicators (buds number, bud length and stem length) and a significant increase in (bud diameter and branches number) in the propagation and elongation stages. Also, the chemical growth indicators recorded an increase in the chemical content (abscisic acid and proline), the effect of Sodium chloride was negative on photosynthesis, as the chlorophyll content gradually decreased with increasing sodium chloride concentration. [ABSTRACT FROM AUTHOR]
- Published
- 2022