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The article discusses the study that examines the impact of supplementation of hormones, epidermal growth factor (EGF), insulin and β-mercaptoethanol on in-vitro maturation medium for in-vitro maturation of carpine oocytes. The study found significantly enhanced in-vitro maturation rate of carpine oocytes by EGF supplementation along with hormones. Higher embryo development competence was noted with addition of hormones, hormones with β-mercaptoethanol, and hormones with insulin.

The article discusses a study of how the cleavage rate (CR) and embryo development (ED) of in-vitro matured and fertilized goat oocytes could be affected by granulosa cell monolayer (GCM) and oviductal epithelial cells (OEC) co-culture. The researchers determined the fertilized oocytes in ED media over GCM and OEC for CR and ED using a steriozoom microscope. They found a similar co-culture behavior between GCM and OEC for CR and ED of both samples.

The article discusses clinical research which aims to evaluate the effects of granulosa cells monolayer and oviductal epithelial cells co-culture on cleavage rate and embryo developmental competence of in-vitro fertilized goat oocytes. Cumulus oocyte complexes (COCs) were collected from goat ovaries in prepubertal and pubertal stages. The chi square analysis was used in examining the difference between cleavage rates and embryo development stages.

The article discusses a study which examined the effects of in-vitro maturation and cleavage rate of prepubertal and pubertal goat oocytes in maturation media TCM-199. Based on its findings, the maturation rates of oocytes for prepubertal and pubertal animals were 89.80 and 89.77%, respectively. It mentions the objective of the study which was for the production of oocytes basically required for in-vitro culture and other related biotechnology works.

The article presents a study which examined the effects of different supplementation in the basic maturation media on in-vitro fertilization and cleavage rate. Statistical analysis between prepubertal and pubertal cleaved oocytes showed a significant difference between the cleavage rates of two different groups of collected oocytes. In this study, centrifugation technique was used for sperm preparations and cleavage rate ranging between 28.68 to 44.27 percent in different supplemented media was achieved, which is lower than to the centrifugation process of sperm preparation as used by Palomo et al (1999).

The article reports on a study which aimed to evaluate the effects of different supplementation in the basic media (TCM-199) on in-vitro maturation and cleavage rate of pubertal goat oocytes. An overview on caprine in-vitro embryo production is provided along with the significance of oocytes collected from ovaries of goats. The collection of caprine follicular fluid are presented as well as the oocytes maturation. The results of the statistical analysis are discussed using normal deviate test.

The article discusses a study on the effects of supplements in the basic media (TCM-199) on in-vitro maturation, fertilization and cleavage rate of prepubertal goat oocytes. Ovaries from prepubertal goats were collected and placed in normal saline solution before being subjected to oocytes recovery. Studies were also made on in-vitro fertilization (in Fert-TALP media) and cleavage rate of in-vitro matured oocytes. It concludes that supplements or additives affects the maturation rate of prepubertal goat oocytes.

The article presents a study which examined the influence of culture media on in-vitro fertilization of goat oocytes. Ovaries were obtained from local abattoir and transported within four hours to the laboratory in warm saline containing 100 IU penicillin-G and 100 micrograms streptomycin sulphate/milliliter. Cumulus oocyte complexes retrieved by follicle puncture technique were chosen for in-vitro maturation of which 489 were subsequently utilized for in-vitro fertilization and culture. The study concluded that the utilization of modified Tyrode's albumin lactate pyruvate medium (mTALP) as fertilization medium have significantly increased the cleavage rate of in-vitro matured goat oocytes as compared to Brackett and Oliphant (BO) and modified synthetic oviductal fluid (mSOF) medium.