1. Distinctin improves the efficacies of glycopeptides and betalactams against staphylococcal biofilm in an experimental model of central venous catheter infection
- Author
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Irma Testa, Agnese Della Vittoria, Andrea Scaloni, Giorgio Scalise, Roberto Ghiselli, Fiorenza Orlando, Oscar Cirioni, Andrea Giacometti, Federico Mocchegiani, Carmela Silvestri, Vittorio Saba, and Giovanni Renzone
- Subjects
Male ,Staphylococcus aureus ,Time Factors ,medicine.drug_class ,medicine.medical_treatment ,Antibiotics ,Antimicrobial peptides ,Colony Count, Microbial ,Biomedical Engineering ,Bacterial Adhesion ,Catheterization ,Microbiology ,Biomaterials ,In vivo ,medicine ,Animals ,Rats, Wistar ,business.industry ,Metals and Alloys ,Biofilm ,Staphylococcal Infections ,medicine.disease ,In vitro ,Glycopeptide ,Anti-Bacterial Agents ,Rats ,Disease Models, Animal ,Biofilms ,Bacteremia ,Ceramics and Composites ,Equipment Contamination ,Peptides ,business ,Central venous catheter - Abstract
The ability of microorganisms to adhere to medical implants is a problem of high visibility and has been focused in numerous investigations. To assess the efficacy of distinctin and conventional antibiotics in the treatment of central venous catheter in vitro and in vivo studies were performed. The in vitro susceptibility assay was performed against S. aureus biofilms developed on 96-well polystyrene tissue culture plates. Efficacy studies were performed in a rat model of CVC infection. Twenty-four hours after implantation, the catheters were filled with distinctin. Thirty minutes later, rats were challenged via the CVC with S. aureus. Administration of antibiotics into the CVC at a concentration equal to the MBC for adherent cells, or at 1024 μg/mL began 24 h later. The killing activities of all antibiotics against adherent bacteria were at least four- to eightfold lower than against freely growing cells. When antibiotics were used in distinctin pretreated wells, they showed a significant increase of activity. The in vivo studies showed that when CVCs were pretreated with distinctin biofilm bacterial load was further decreased to 101 CFU/mL and bacteremia was not detected. Distinctin displays potential as an adjunctive agent to antibiotics in the treatment of CVC-related infections. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res 2007
- Published
- 2007