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Your search keyword '"Xiao-jian Yao"' showing total 2 results
Start Over Author "Xiao-jian Yao" Topic immunology
2 results on '"Xiao-jian Yao"'

1. Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs

Author

Maggie Jing Ouyang, Zhujun Ao, Titus A. Olukitibi, and Xiao-Jian Yao

Subjects
Immunology, Microbiology, Science (General), Q1-390
Abstract

Summary: The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and SARS-CoV-2-based virus-like particles and then evaluate the inflammatory cytokines’ protein and mRNA levels produced in human macrophages by S of SARS-CoV-2 original strain and Delta variant. This protocol is applicable in evaluating S from different emerging variants.For complete details on the use and execution of this protocol, please refer to Ao et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published
2023
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2. Isolation of drug-resistant variants of HIV-1 from patients on long-term zidovudine therapy

Author

Ronald Rooke, Michel Tremblay, Hugo Soudeyns, Lucie DeStephano, Xiao-Jian Yao, Mary Fanning, Julio S.G. Montaner, Michael OʼShaughnessy, Karen Gelmon, Chris Tsoukas, John Gill, John Ruedy, and Mark A. Wainberg

Subjects
Drug, business.industry, media_common.quotation_subject, Immunology, Drug resistance, Virology, Phenotype, Peripheral blood mononuclear cell, Virus, Zidovudine, Tissue culture, Infectious Diseases, Immunology and Allergy, Medicine, business, Nucleoside, media_common, medicine.drug
Abstract

We determined whether drug-resistant variants of HIV-1 could be isolated from the peripheral blood mononuclear cells of 20 individuals with HIV infection (Centers for Disease Control groups II and III) on long-term zidovudine (AZT) therapy. Toward this end, zidovudine (10 microM) has been included in the tissue culture medium used to isolate HIV-1. Under these circumstances, virus with a zidovudine-resistant phenotype was successfully obtained in five out of 20 cases. This property of drug resistance appeared to be stable, and did not disappear upon extended replication of such virus in the absence of drug pressure. Drug-resistant virus could also be isolated from these subjects on subsequent occasions, but was not present in samples obtained prior to therapy. Replication of these zidovudine-resistant isolates in tissue culture was inhibited by each of four other nucleoside analogues. Thus, other drugs may be useful in controlling selective zidovudine-resistant variants of HIV-1.

Published
1989

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