1. [Polysaccharides from Nerium oleander: structure and biological activity]
- Author
-
B M, Müller, F, Rosskopf, D H, Paper, J, Kraus, and G, Franz
- Subjects
Male ,Mice, Inbred C3H ,Magnetic Resonance Spectroscopy ,Plants, Medicinal ,Cell Survival ,Plant Extracts ,Hydrolysis ,Macrophages ,In Vitro Techniques ,Antineoplastic Agents, Phytogenic ,Methylation ,Mice ,Structure-Activity Relationship ,Phagocytosis ,Polysaccharides ,Tumor Cells, Cultured ,Animals ,Immunologic Factors ,Female ,Protease Inhibitors ,Lymphocytes ,Mitogens ,Limulus Test - Abstract
A water extraction of crushed leaves of Nerium oleander yielded 2.3% of a crude polysaccharide. The main fraction (67%) represents a pectic polysaccharide mainly composed of galacturonic acid besides rhamnose, arabinose and galactose. The polysaccharide structure was characterized by NMR, mild acid- and pectinase treatment combined with GC-MS analyses. In vivo tests for a possible antitumor activity did not result in a significant action. Investigation of immunomodulating activity brought some indications for mitogenic activity and a weak macrophage-mediated cytotoxicity. Increase of phagocytosis could not be doubtlessly assigned to the polysaccharide due to the high amount of endotoxin in the homogenous fraction OLE 2.
- Published
- 1991