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The article reports that the immunotherapeutic strategies are emerging as novel therapeutic approaches in multiple myeloma with several monoclonal antibodies being in advanced stages of clinical development. CD38 targeting antibodies are promising and some of them are in the market in many countries. Therapeutic CD38-targeting antibodies were shown to interfere with pretransfusion laboratory tests due to weak expression of CD38 on human erythrocytes. more...

The article focuses on the typing and matching strategies in patients on anti-CD38 monoclonal therapy. The cells do not clean completely the interference that dara produce. Dara interference is heterogeneous on the different screening cells. They manage these results with an haematologist supervision, using common sense to transfuse patients without delay. more...

Detry G, Drénou B, Ferrant A, Theate I, Michaux L, Scheiff JM, Latinne D, Leveugle P, Mazzon AM, Deneys V. Tracking the follicular lymphoma cells in flow cytometry: characterisation of a new useful antibody combination.Eur J Haematol 2004: 73: 325–331.© Blackwell Munksgaard 2004.Objectives: Follicular lymphoma (FL) is the most common adult non-Hodgkin's lymphoma. Diagnosis is based on morphology and can be confirmed by cytogenetic, flow cytometry (FCM) or molecular studies. Despite all these complementary approaches, diagnosis sometimes remains difficult. The purpose of the present work was to characterise the expression of new specific follicular cells markers which allows us to target specifically the abnormal FL cell population in FCM.Methods: A total of 153 samples from healthy subjects and from patients with chronic B-cell lymphoproliferative disorders were analysed by FCM in the same conditions for purpose of comparison.Results: We showed that CD44 is weakly expressed in FL cells compared with peripheral blood mononuclear cell from normal blood donors and others cells from B lymphoproliferative diseases. We nevertheless observed bone marrow samples where some immature B-cell population express CD44 with lower fluorescence intensity. Therefore, we developed a double antibody combination, using CD44 and CD38, which allowed us to separate the normal immature cells from the pathological population using FCM.Conclusion: This new phenotypic approach offers an accurate (sensitivity and specificity of 93% and 96%, respectively), fast and low sample consuming method for the diagnosis of FL. [ABSTRACT FROM AUTHOR] more...

Reports on the application of the antibody Rituximab in the treatment of cold agglutinin diseases (CAD). Description of activities of this antibody; Presentation of case study of a patient regarding the application of Rituximab in the treatment of CAD. more...