1. Proteomic analysis of known and candidate rice allergens between non-transgenic and transgenic plants
- Author
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Satoh, Rie, Nakamura, Rika, Komatsu, Akira, Oshima, Masahiro, and Teshima, Reiko
- Subjects
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PROTEOMICS , *ALLERGENS , *TRANSGENIC rice , *CARRIER proteins , *PEROXIDASE , *IMMUNOGLOBULIN E , *THIOCYANATES , *POLYACRYLAMIDE gel electrophoresis - Abstract
Abstract: Salt-soluble proteins extracted from non-transgenic and transgenic rice were evaluated for the presence of known and potential allergens by proteomic techniques. The salt-soluble proteins were extracted, separated by 1D and 2D electrophoresis, and analyzed by Western blotting. 1D immunoblot analysis with patients’ sera revealed few qualitative differences between the IgE-binding proteins of the non-transgenic and transgenic rice. 1D immunoblot with antigen-specific-animal sera revealed no qualitative or quantitative differences in two known allergens, RAG2 and glyoxalase I, between non-transgenic and transgenic rice. Multiple spots containing known and novel IgE-binding proteins were detected among the salt-soluble proteins of non-transgenic rice by 2D immunoblotting. Two globulin-like proteins, a 52kDa protein and a 63kDa protein, were identified as novel IgE-binding proteins that are candidates for rice allergens. These globulin-like proteins were homologous to Cupin superfamily allergens. Quantitative analysis of 19, 52, and 63kDa globulins with protein-specific-animal sera showed no significant differences in the expression of these proteins between the transgenic rice and non-transgenic rice. These results indicate that none of the known or novel endogeneous IgE-binding proteins detected in this study appear to be altered by genetic modification. [Copyright &y& Elsevier]
- Published
- 2011
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