1. Boosting the biocatalytic precipitation with enzyme-loaded liposomes: Toward a general platform for amplified photoelectrochemical immunoassay.
- Author
-
Yu LM, Liu YL, Zhu LB, Shen Q, Han DM, Qu P, and Zhao WW
- Subjects
- Biocatalysis, Horseradish Peroxidase chemistry, Liposomes chemistry, Liposomes metabolism, Particle Size, Photochemical Processes, Surface Properties, Biosensing Techniques, Electrochemical Techniques, Horseradish Peroxidase metabolism, Immunoassay
- Abstract
Liposome-assisted photoelectrochemical (PEC) bioanalysis represents one of the latest frontiers in the arena of PEC bioanalysis. This work reports a general enzyme-amplified liposomal PEC bioanalysis protocol via the use of enzyme-loaded liposomes to boost the biocatalytic precipitation (BCP) effect. In the representative system, the horseradish peroxidase (HRP)-loaded liposome (HRPLL) and the Au nanoclusters (NCs)/Au nanoparticles (NPs)/TiO
2 nanotubes (NTs) framework (AATF) were used as liposomal label and photoelectrode, respectively. In the detection, the sandwich immunocomplex reaction was accomplished in a 96-well plate to confine the HRPLL label, which was then lysed to release the HRP molecules to initiate the BCP process. Due to the amplified formation of HRP-induced BCP on the AATF scaffold, the photo-current response correlated closely with the immunorecognition process and the analyte could be detected very sensitively. This work features the first integration of enzyme-loaded liposomes and the BCP for sensitive PEC bioanalysis, which to our knowledge has not been reported. With the use of various other enzymes, this work could serve as a general basis for the PEC bioanalysis of numerous other target of interest., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF