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This study evaluated the efficiency of differently prepared vaccines against Aeromonas hydrophila in the hybrid surubim ( Pseudoplatystoma corruscans × P. reticulatum). Survival and haemato-immunological parameters were compared between the treatments: non-vaccinated fish (C); bacterin-vaccinated fish (B); bacterin plus oral booster vaccinated fish (B+O); bacterin and toxoid-vaccinated fish (B+T) and bacterin, toxoid and oral booster-vaccinated fish (B+T+O). Fourteen-days vaccinated fish from B+O and B+T+O were fed with an oral booster for 4 days. After 1 week, the fish were intraperitoneally challenged with 2 × 108 CFU mL−1 of A. hydrophila. Fish from the treatment B+T+O showed the lowest cumulative mortality (11.36%) 96 h after challenge, compared with other treatments (22.72-44.04%), and a relative survival of 74%. Serum immunoglobulin in B+T+O fish was higher than in other treatments. All vaccinated fish showed an increased agglutination titre when compared with non-vaccinated fish, both before and after challenge. Fish fed with oral booster showed an increase in phagocytic percentage before and after challenge. It can be inferred that the oral booster vaccination was efficient in reducing mortality in hybrid surubim by enhancing the response against haemorrhagic septicaemia due to A. hydrophila infection. [ABSTRACT FROM AUTHOR]