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Chito-oligosaccharide is a class of small molecule compounds with good water solubility, high biocapacitivity, low toxicity, and easy intestinal absorption, which are prepared by chitosan deacetylation and have various physiological effects such as antibacterial, antioxidant, and anti-inflammatory. As a new feed additive, chito-oligosaccharide has a significant role in improving the production of animals, which can effectively improve production performance and product quality, which is in line with the development trend of healthy breeding. Therefore, the paper reviews the physicochemical properties and physiological functions of chito-oligosaccharides, and expounds the role of chito-oligosaccharides in broiler production from meat quality, immune function, growth performance, and intestinal barrier, aiming to provide a reference for chito-oligosaccharides to be used as a green and efficient feed additive in broiler production. [ABSTRACT FROM AUTHOR]