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This study aimed to develop simplified models for rapid and nondestructive monitoring myoglobin contents (DeoMb, MbO 2 and MetMb) during refrigerated storage of Tan sheep based on a hyperspectral imaging (HSI) system in the spectral range of 400-1000 nm. Partial least squares regression (PLSR) and least-squares support vector machines (LSSVM) were applied to correlate the spectral data with the reference values of myoglobin contents measured by a traditional method. In order to simplify the LSSVM models, competitive adaptive reweighted sampling (CARS) and Interval variable iterative space shrinkage approach (iVISSA) were used to select key wavelengths. The new CARS-LSSVM models of DeoMb and MbO 2 yielded good results, with R 2 p of 0.810 and 0.914, RMSEP of 1.127 and 2.598, respectively. The best model of MetMb was new iVISSA-CARS-LSSVM, with an R 2 p of 0.915 and RMSEP of 2.777. The overall results from this study indicated that it was feasible to predict myoglobin contents in Tan sheep using HSI., Competing Interests: Declaration of Competing Interest There are no conflicts of interest., (Copyright © 2019. Published by Elsevier Ltd.)

Hyperspectral imaging (HSI), a non-destructive method combining image and spectral techniques to obtain image and spectral information of target objects, and qualitative and quantitative analyses using spectral data, has been widely used in agricultural product detection. This study was conducted to assess the feasibility of using HSI (900–1700 nm) for rapid determination of myoglobin contents (DeoMb, OxyMb and MetMb) in nitrite-cured mutton during refrigerated storage. The data were analyzed using evolutionary computing methods, including partial least squares regression (PLSR) and least squares support vector machines (LSSVM). Corresponding feature wavelengths were then selected by using competitive adaptive reweighted sampling (CARS), variable combination population analysis (VCPA) and interval variable iterative space shrinkage approach (iVISSA) respectively. The simplified iVISSA-PLSR model showed the best performance for determining DeoMb with R p of 0.9017 and RMSEP of 2.3509. In addition, the best model for OxyMb was CARS-PLSR, with R p of 0.9661 and RMSEP of 2.3762. Moreover, the CARS-LSSVM model showed a better performance with a higher R p (0.8931) and a lower RMSEP (3.2743) for MetMb. Furthermore, the overall performance of wavelength selection methods in terms of CARS (R pDeoMb ≥ 0.8943, R pOxyMb ≥ 0.9607, R pMetMb ≥ 0.8910) and iVISSA (R pDeoMb ≥ 0.8976, R pOxyMb ≥ 0.9568, R pMetMb ≥ 0.8880) were equivalent and slightly better than VCPA (R pDeoMb ≥ 0.8711, R pOxyMb ≥ 0.9573, R pMetMb ≥ 0.8808). The results demonstrated that effective wavelength selection method can improve the performance of HSI system for the determination of myoglobin contents in comparison to full wavelength. • Feature wavelengths were selected by CARS, VCPA, iVISSA and iVISSA-CARS. • PLSR and LSSVM models were developed to measure myoglobin contents (DeoMb, OxyMb and MetMb) of nitrite-cured mutton. • The correlation coefficient of simpled model is acceptable for measuring myoglobin contents. [ABSTRACT FROM AUTHOR]

The amount and chemical state of myoglobin are often relevant to meat color, which is one of the important characteristics of meat freshness and wholesomeness. To precisely determine OxyMb and MetMb contents in mutton, the feasibility of optimization detection technology of spectral image fusion was explored. Three methods were used to select characteristic wavelengths while textural features of muscle tissue were derived. Compared among all models, LSSVM models coupled with spectra and textural parameters all performed better than these PLSR models. The iVISSA-COR-LSSVM model for OxyMb prediction achieved a good performance of R2 C = 0.9525, RMSEC = 2.2955, R2 P = 0.8956, and RMSEP = 3.0036. The more effective prediction performances emerged from VCPA-IRIV-COR-LSSVM model with R2 C = 0.8809, RMSEC = 2.2009, R2 P = 0.8353, and RMSEP = 3.2096 for MetMb. In final, the pseudo color maps of OxyMb and MetMb distribution were generated. This study demonstrated that the data fusion method furnishes a new approach to rapidly evaluate myoglobin content. • Better results were obtained by iVISSA and VCPA-IRIV identified wavebands. • Data fusion was used for enhancing hyperspectral prediction performance of myoglobin value. • LSSVM models built for predicting OxyMb and MetMb contents showed good results. • Distribution maps of OxyMb and MetMb values were visualized. [ABSTRACT FROM AUTHOR]