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Start Over Author "Floccari ME" Topic hydroxybutyrates
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1. [Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)].

Author

Floccari ME, Tiscornia G, Palmada F, and Méndez BS

Subjects
Bacillus megaterium isolation & purification, Bacillus megaterium metabolism, Centrifugation, Density Gradient, Spores, Bacterial, Bacillus megaterium genetics, Hydroxybutyrates metabolism, Polyesters metabolism
Abstract

Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.

Published
1991

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