1. Long non-coding RNA ANRIL alleviates H 2 O 2 -induced injury by up-regulating microRNA-21 in human lens epithelial cells.
- Author
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Du S, Shao J, Qi Y, Liu X, Liu J, and Zhang F
- Subjects
- AMP-Activated Protein Kinases physiology, Cataract etiology, Cataract genetics, Cells, Cultured, Epithelial Cells drug effects, Humans, Lens, Crystalline metabolism, Lens, Crystalline pathology, Oxidative Stress, Up-Regulation, beta Catenin physiology, Hydrogen Peroxide toxicity, Lens, Crystalline drug effects, MicroRNAs physiology, RNA, Long Noncoding physiology
- Abstract
The accurate role of ANRIL in cataract is poorly understood. We aimed to reveal the effects of ANRIL on H
2 O2 -treated HLECs, SRA01/04, as well as the regulatory mechanisms. Oxidative stress model of HLECs was induced by H2 O2 . Cell injury was evaluated according to cell proliferation, apoptosis and DNA damage using CCK-8 assay/flow cytometry and TUNEL assays/γH2AX staining. Expressions of ANRIL and miR-21 in HLECs were determined by RT-qPCR. The effects of miR-21, miR-34a and miR-122-5p inhibition as well as AMPK and β-catenin on HLECs with ANRIL overexpression and H2 O2 stimulation were analyzed. In vivo experiment was performed via RT-qPCR. H2 O2 repressed proliferation and induced apoptosis or DNA damage in HLECs. Those alterations induced by H2 O2 were attenuated by ANRIL overexpression. MiR-21 was positively regulated by ANRIL, and both of them were repressed in H2 O2 -induced HLECs and cataract patient tissues. Inhibition of miR-21 but not miR-34a or miR-122-5p reversed the effects of ANRIL on H2 O2 -treated HLECs. Phosphorylation of AMPK and expression of β-catenin were increased by ANRIL via regulating miR-21. AMPK and β-catenin affected beneficial function of ANRIL-miR-21 axis.Therefore, lncRNA ANRIL attenuated H2 O2 -induced cell injury in HELCs via up-regulating miR-21 via the activation of AMPK and β-catenin.- Published
- 2020
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