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Hydrogels are three-dimensional cross-linked polymeric networks capable of a large amount of fluid retention in their structure. Hydrogel outputs manufactured using additive manufacturing technologies are exposed to water loss, which may change their original shape and dimensions. Therefore, the possibility of retaining water is important in such a structure. In this manuscript, kinetic analysis of water evaporation from sodium alginate-based hydrogels exposed to different environmental conditions such as different temperatures (7 and 23 °C) and ambient humidity (45, 50 and 95%) has been carried out. The influence of the cross-linking method (different calcium chloride concentration—0.05, 0.1 and 0.5 M) of sodium alginate and cross-linking time on the water loss was also considered. Studies have shown that a decrease in the temperature and increase in the storage humidity can have a positive effect on the water retention in the structure. The storage conditions that led to the least weight and volume loss were T 7 °C and 95% humidity. These experiments may help in selecting the appropriate hydrogel preparation method for future applications, as well as their storage conditions for minimum water loss and, consequently, the least change in dimensions and shape. [ABSTRACT FROM AUTHOR]