1. The potential of the novel NAD + supplementing agent, SNH6, as a therapeutic strategy for the treatment of Friedreich's ataxia.
- Author
-
Chiang S, Kalinowski DS, Dharmasivam M, Braidy N, Richardson DR, and Huang MLH
- Subjects
- Adenosine Triphosphate metabolism, Aldehydes pharmacology, Animals, Cardiomyopathies metabolism, Cell Line, Creatine Kinase, MM Form genetics, Disease Models, Animal, Friedreich Ataxia metabolism, Hydrazones pharmacology, Iron metabolism, Iron Chelating Agents pharmacology, Iron-Binding Proteins genetics, Mice, Inbred C57BL, Mice, Knockout, Mitochondria, Heart drug effects, Mitochondria, Heart metabolism, Rats, Frataxin, Aldehydes therapeutic use, Cardiomyopathies drug therapy, Friedreich Ataxia drug therapy, Hydrazones therapeutic use, Iron Chelating Agents therapeutic use, NAD metabolism
- Abstract
Friedreich's ataxia (FA) is due to deficiency of the mitochondrial protein, frataxin, which results in multiple pathologies including a deadly, hypertrophic cardiomyopathy. Frataxin loss leads to deleterious accumulations of redox-active, mitochondrial iron, and suppressed mitochondrial bioenergetics. Hence, there is an urgent need to develop innovative pharmaceuticals. Herein, the activity of the novel compound, 6-methoxy-2-salicylaldehyde nicotinoyl hydrazone (SNH6), was assessed in vivo using the well-characterized muscle creatine kinase (MCK) conditional frataxin knockout (KO) mouse model of FA. The design of SNH6 incorporated a dual-mechanism mediating: (1) NAD
+ -supplementation to restore cardiac bioenergetics; and (2) iron chelation to remove toxic mitochondrial iron. In these studies, MCK wild-type (WT) and KO mice were treated for 4-weeks from the asymptomatic age of 4.5-weeks to 8.5-weeks of age, where the mouse displays an overt cardiomyopathy. SNH6-treatment significantly elevated NAD+ and markedly increased NAD+ consumption in WT and KO hearts. In SNH6-treated KO mice, nuclear Sirt1 activity was also significantly increased together with the NAD+ -metabolic product, nicotinamide (NAM). Therefore, NAD+ -supplementation by SNH6 aided mitochondrial function and cardiac bioenergetics. SNH6 also chelated iron in cultured cardiac cells and also removed iron-loading in vivo from the MCK KO heart. Despite its dual beneficial properties of supplementing NAD+ and chelating iron, SNH6 did not mitigate cardiomyopathy development in the MCK KO mouse. Collectively, SNH6 is an innovative therapeutic with marked pharmacological efficacy, which successfully enhanced cardiac NAD+ and nuclear Sirt1 activity and reduced cardiac iron-loading in MCK KO mice. No other pharmaceutical yet designed exhibits both these effective pharmacological properties., Competing Interests: Declaration of Competing Interest None., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF