1. The effect of hydrazide linkers on hyaluronan hydrazone hydrogels.
- Author
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Šedová P, Buffa R, Šilhár P, Kovářová L, Vágnerová H, Bednařík J, Basarabová I, Hejlová L, Ščigalková I, Šimek M, and Velebný V
- Subjects
- Acylation, Animals, Biocompatible Materials chemical synthesis, Biocompatible Materials chemistry, Biocompatible Materials toxicity, Cross-Linking Reagents chemical synthesis, Cross-Linking Reagents toxicity, Elastic Modulus, Hyaluronic Acid chemical synthesis, Hyaluronic Acid toxicity, Hydrazines chemical synthesis, Hydrazines toxicity, Hydrazones chemical synthesis, Hydrazones toxicity, Hydrogels chemical synthesis, Hydrogels toxicity, Hydrogen-Ion Concentration, Kinetics, Mice, Swiss 3T3 Cells, Cross-Linking Reagents chemistry, Hyaluronic Acid analogs & derivatives, Hydrazines chemistry, Hydrazones chemistry, Hydrogels chemistry
- Abstract
The effect of hydrazide linkers on the formation and mechanical properties of hyaluronan hydrogels was intensively evaluated. The reaction kinetics of hydrazone formation was monitored by NMR spectroscopy under physiological conditions where polyaldehyde hyaluronan (unsaturated: ΔHA-CHO, saturated: HA-CHO) was reacted with various hydrazides to form hydrogels. Linear (adipic, oxalic dihydrazide) and branched (N,N´,N´´-tris(hexanoylhydrazide-6-yl)phosphoric triamide and 4-arm-PEG hydrazide) hydrazides were compared as crosslinking agents. The mechanical properties of hydrogels were also modified by attaching a hydrophobic chain to HA-CHO; however, it was found that this modification did not lead to an increase in hydrogel stiffness. Cytotoxicity tests showed that all tested hydrazide crosslinkers reduced the viability of cells only slightly, and that the final hyaluronan hydrogels were non-toxic materials., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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