10 results on '"Zhongling Luo"'
Search Results
2. AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX–MDM2 complex
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John B. Little, Xiang Chen, Zhi-Min Yuan, Zhongling Luo, Anna de Polo, and Casimiro Gerarduzzi
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0301 basic medicine ,Indoles ,MDMX ,Transcription, Genetic ,Cell Cycle Proteins ,p38 Mitogen-Activated Protein Kinases ,Receptor tyrosine kinase ,Phosphoserine ,03 medical and health sciences ,Cell Line, Tumor ,Proto-Oncogene Proteins ,Genetics ,Humans ,Phosphorylation ,Melanoma ,Molecular Biology ,Cell Nucleus ,Sulfonamides ,biology ,Protein Stability ,Chemistry ,GAS6 ,Cyclin-dependent kinase 4 ,Kinase ,Nuclear Proteins ,Receptor Protein-Tyrosine Kinases ,Proto-Oncogene Proteins c-mdm2 ,Cell Biology ,General Medicine ,Axl Receptor Tyrosine Kinase ,Cyclin-Dependent Kinases ,Cell biology ,Protein Transport ,030104 developmental biology ,Gene Expression Regulation ,Vemurafenib ,Gene Knockdown Techniques ,Proteolysis ,biology.protein ,Original Article ,Cisplatin ,Tumor Suppressor Protein p53 ,Signal transduction ,Tyrosine kinase ,Signal Transduction - Abstract
Deregulation of the tyrosine kinase signalling is often associated with tumour progression and drug resistance, but its underlying mechanisms are only partly understood. In this study, we investigated the effects of the receptor tyrosine kinase AXL on the stability of the MDMX-MDM2 heterocomplex and the activity of p53 in melanoma cells. Our data demonstrated that AXL overexpression or activation through growth arrest-specific 6 (Gas6) ligand stimulation increases MDMX and MDM2 protein levels and decreases p53 activity. Upon activation, AXL stabilizes MDMX through a post-translational modification that involves phosphorylation of MDMX on the phosphosite Ser314, leading to increased affinity between MDMX and MDM2 and favouring MDMX nuclear translocation. Ser314 phosphorylation can also protect MDMX from MDM2-mediated degradation, leading to stabilization of the MDMX-MDM2 complex. We identified CDK4/6 and p38 MAPK as the two kinases mediating AXL-induced modulation of the MDMX-MDM2 complex, and demonstrated that suppression of AXL, either through siRNA silencing or pharmacological inhibition, increases expression levels of p53 target genes P21, MDM2, and PUMA, improves p53 pathway response to chemotherapy, and sensitizes cells to both Cisplatin and Vemurafenib. Our findings offer an insight into a novel signalling axis linking AXL to p53 and provide a potentially druggable pathway to restore p53 function in melanoma.
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- 2016
3. The Application of Deep Learning in the Risk Grading of Skin Tumors for Patients Using Clinical Images
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Wei Fan, Xiaoyu He, Mingliang Chen, Zhongling Luo, Bin Xie, Kai Huang, Fangfang Li, Shuang Zhao, Jie Li, Yi Li, Weihong Huang, Zhe Wu, Xinyu Zhao, Juan Su, and Xian Wu
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Seborrheic keratosis ,medicine.medical_specialty ,Skin Neoplasms ,Databases, Factual ,020205 medical informatics ,Junctional nevus ,Medicine (miscellaneous) ,Health Informatics ,02 engineering and technology ,Malignancy ,Deep Learning ,Health Information Management ,0202 electrical engineering, electronic engineering, information engineering ,Intradermal Nevus ,Humans ,Medicine ,Basal cell carcinoma ,Melanoma ,Grading (tumors) ,business.industry ,Actinic keratosis ,Lipoma ,medicine.disease ,Dermatology ,Area Under Curve ,Neural Networks, Computer ,Patient Participation ,business ,Cell Phone ,Information Systems - Abstract
According to diagnostic criteria, skin tumors can be divided into three categories: benign, low degree and high degree malignancy. For high degree malignant skin tumors, if not detected in time, they can do serious harm to patients' health. However, in clinical practice, identifying malignant degree requires biopsy and pathological examination which is time costly. Furthermore, in many areas, due to the severe shortage of dermatologists, it's inconvenient for patients to go to hospital for examination. Therefore, an easy to access screening method of malignant skin tumors is needed urgently. Firstly, we spend 5 years to build a dataset which includes 4,500 images of 10 kinds of skin tumors. All instances are verified pathologically thus trustworthy; Secondly, we label each instance to be either low-risk, high-risk or dangerous in which Junctional nevus, Intradermal nevus, Dermatofibroma, Lipoma and Seborrheic keratosis are low-risk, Basal cell carcinoma, Bowen's disease and Actinic keratosis are high-risk, Squamous cell carcinoma and Malignant melanoma are dangerous; Thirdly, we apply the Xception architecture to build the risk degree classifier. The area under the curve (AUC) for three risk degrees reach 0.959, 0.919 and 0.947 respectively. To further evaluate the validity of the proposed risk degree classifier, we conduct a competition with 20 professional dermatologists. The results showed the proposed classifier outperforms dermatologists. Our system is helpful to patients in preliminary screening. It can identify the patients who are at risk and alert them to go to hospital for further examination.
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- 2019
4. Epigallocatechin-3-gallate(EGCG) suppresses melanoma cell growth and metastasis by targeting TRAF6 activity
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Zhou Lei, Juan Su, Xu Zhang, Zunnan Huang, Jianglin Zhang, Cong Peng, Youyou Zhou, Xiang Chen, Zhongling Luo, and Weiqi Zeng
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0301 basic medicine ,Lung Neoplasms ,Skin Neoplasms ,Protein Conformation ,Catechin ,0302 clinical medicine ,NF-KappaB Inhibitor alpha ,Ubiquitin ,Cell Movement ,Medicine ,Enzyme Inhibitors ,Phosphorylation ,Mice, Inbred BALB C ,biology ,Melanoma ,Intracellular Signaling Peptides and Proteins ,NF-kappa B ,food and beverages ,MAP Kinase Kinase Kinases ,Tumor Burden ,Ubiquitin ligase ,Gene Expression Regulation, Neoplastic ,Molecular Docking Simulation ,Oncology ,030220 oncology & carcinogenesis ,Tumor necrosis factor alpha ,TRAF6 ,Protein Binding ,Signal Transduction ,Research Paper ,P50 ,Ubiquitin-Protein Ligases ,Transfection ,ubiquitination ,complex mixtures ,Structure-Activity Relationship ,03 medical and health sciences ,Cell Line, Tumor ,melanoma ,Animals ,Humans ,Neoplasm Invasiveness ,Cell Proliferation ,TNF Receptor-Associated Factor 6 ,Cell growth ,business.industry ,medicine.disease ,Antineoplastic Agents, Phytogenic ,IκBα ,HEK293 Cells ,030104 developmental biology ,Ubiquitin-Conjugating Enzymes ,Immunology ,biology.protein ,Cancer research ,business ,EGCG - Abstract
// Jianglin Zhang 1, 2, * , Zhou Lei 1, 2, * , Zunnan Huang 3, * , Xu Zhang 1, 2 , Youyou Zhou 1, 2 , Zhongling Luo 1, 2 , Weiqi Zeng 1, 2 , Juan Su 1, 2 , Cong Peng 1, 2 , Xiang Chen 1, 2 1 The Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China 2 Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China 3 Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong, China * These authors have contributed equally to this work Correspondence to: Cong Peng, email: pengcongxy@csu.edu.cn Xiang Chen, email: chenxiangck@126.com Keywords: EGCG, TRAF6, ubiquitination, melanoma Received: August 24, 2016 Accepted: October 07, 2016 Published: October 24, 2016 ABSTRACT TRAF6 (TNF Receptor-Associated Factor 6) is an E3 ubiquitin ligase that contains a Ring domain, induces K63-linked polyubiquitination, and plays a critical role in signaling transduction. Our previous results demonstrated that TRAF6 is overexpressed in melanoma and that TRAF6 knockdown dramatically attenuates tumor cell growth and metastasis. In this study, we found that EGCG can directly bind to TRAF6, and a computational model of the interaction between EGCG and TRAF6 revealed that EGCG probably interacts with TRAF6 at the residues of Gln54, Gly55, Asp57 ILe72, Cys73 and Lys96. Among these amino acids, mutation of Gln54, Asp57, ILe72 in TRAF6 could destroy EGCG bound to TRAF6, furthermore, our results demonstrated that EGCG significantly attenuates interaction between TRAF6 and UBC13(E2) and suppresses TRAF6 E3 ubiquitin ligase activity in vivo and in vitro . Additionally, the phosphorylation of IκBα, p-TAK1 expression are decreased and the nuclear translocation of p65 and p50 is blocked by treatment with EGCG, leading to inactivation of the NF-κB pathway. Moreover, EGCG significantly inhibits cell growth as well as the migration and invasion of melanoma cells. Taken together, these findings show that EGCG is a novel E3 ubiquitin ligase inhibitor that could be used to target TRAF6 for chemotherapy or the prevention of melanoma.
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- 2016
5. TRAF6 regulates melanoma invasion and metastasis through ubiquitination of Basigin
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Shuang Zhao, Xiang Chen, Xu Zhang, Wen Tang, Lisha Wu, Zhongling Luo, Juan Su, Cong Peng, Keda Yang, Xuekun Jia, Weiqi Zeng, Chuan Bian Lim, and Lixia Lu
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Male ,0301 basic medicine ,Lung Neoplasms ,Fluorescent Antibody Technique ,Apoptosis ,MMP9 ,Metastasis ,Immunoenzyme Techniques ,Small hairpin RNA ,Mice ,0302 clinical medicine ,Ubiquitin ,Cell Movement ,Tumor Cells, Cultured ,Mice, Inbred BALB C ,biology ,Melanoma ,Oncology ,030220 oncology & carcinogenesis ,Melanocytes ,TRAF6 ,Research Paper ,Blotting, Western ,Mice, Nude ,ubiquitination ,03 medical and health sciences ,melanoma ,Cell Adhesion ,medicine ,Animals ,Humans ,Immunoprecipitation ,Neoplasm Invasiveness ,Cell Proliferation ,TNF Receptor-Associated Factor 6 ,Wound Healing ,Cell growth ,business.industry ,medicine.disease ,invasion and metastasis ,Xenograft Model Antitumor Assays ,030104 developmental biology ,Basigin ,Immunology ,Cancer cell ,biology.protein ,Cancer research ,business - Abstract
// Zhongling Luo 1 , Xu Zhang 1 , Weiqi Zeng 1 , Juan Su 1 , Keda Yang 2 , Lixia Lu 1 , Chuan Bian Lim 3 , Wen Tang 4 , Lisha Wu 5 , Shuang Zhao 1 , Xuekun Jia 1 , Cong Peng 1 , Xiang Chen 1 1 Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China 2 Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, China 3 Department of Genetics and Complex Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA 4 Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan, China 5 Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha, Hunan, China Correspondence to: Cong Peng, e-mail: pengchongpeng@hotmail.com Xiang Chen, e-mail: chenxiangck@126.com Keywords: TRAF6, melanoma, invasion and metastasis, Basigin, ubiquitination Received: September 07, 2015 Accepted: January 02, 2016 Published: January 12, 2016 ABSTRACT TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses. Although studies have shown that TRAF6 has oncogenic activity, the role of TRAF6 in melanoma is unclear. Here, we report that TRAF6 is overexpressed in primary as well as metastatic melanoma tumors and melanoma cell lines. Knockdown of TRAF6 with shRNA significantly suppressed malignant phenotypes including cell proliferation, anchorage-independent cell growth and metastasis in vitro and in vivo . Notably, we demonstrated that Basigin (BSG)/CD147, a critical molecule for cancer cell invasion and metastasis, is a novel interacting partner of TRAF6. Furthermore, depletion of TRAF6 by shRNA reduced the recruitment of BSG to the plasma membrane and K63-linked ubiquitination, in turn, which impaired BSG-dependent MMP9 induction. Taken together, our findings indicate that TRAF6 is involved in regulating melanoma invasion and metastasis, suggesting that TRAF6 may be a potential target for therapy or chemo-prevention in melanoma.
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- 2016
6. Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis
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Cong Peng, Weiqi Zeng, Xuekun Jia, Xiaoyan Huang, Xu Zhang, Xiang Chen, Yanhong Kuang, Juan Su, ShengXi Zhang, Zhongling Luo, and Li Lei
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Keratinocytes ,0301 basic medicine ,Chemokine ,medicine.medical_specialty ,medicine.medical_treatment ,Mice, Transgenic ,Article ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Psoriasis ,medicine ,Animals ,Humans ,STAT3 ,Transcription factor ,Gene knockdown ,Multidisciplinary ,biology ,Interleukins ,medicine.disease ,Dermatology ,HEK293 Cells ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Basigin ,biology.protein ,Cancer research ,Immunoglobulin superfamily ,Epidermis ,Keratinocyte - Abstract
Psoriasis is a chronic inflammatory skin disease characterized by abnormal keratinocyte proliferation and terminal differentiation. Interleukin-22 (IL-22) and the transcription factor Stat3 play pivotal roles in the pathogenesis of psoriasis. CD147 is a transmembrane glycosylation protein that belongs to the immunoglobulin superfamily. Our previous studies have shown that CD147 is a marker of high keratinocyte proliferation and poor keratinocyte differentiation as well as a psoriasis susceptibility gene. The current study demonstrates that CD147 is highly expressed in psoriatic skin lesions. Specific CD147 over-expression in the epidermis of K5-promoter transgenic mice promotes imiquimod (IMQ)-induced psoriasis-like inflammation characterized by acanthosis, granular layer loss and inflammatory cell infiltration. We also found that IL-22 increases CD147 transcription in vitro and in vivo and that Stat3 binds directly to the CD147 promoter between positions −854 and −440, suggesting that CD147 expression is up-regulated in patients with psoriasis through Stat3 activation. In addition, CD147 knockdown dramatically blocks IL-22-mediated Stat3 activation as well as IL-22-induced cytokine, chemokine and antimicrobial factor expression. Together, these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis.
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- 2017
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7. CD147 promotes MTX resistance by immune cells through up-regulating ABCG2 expression and function
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Xiang Chen, Chen Chen, Weiqi Zeng, Shuang Zhao, Yehong Kuang, Shuang Liu, Qingling Li, Shuangyuan Zhou, Lisha Wu, Jianglin Zhang, Juan Su, and Zhongling Luo
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Adult ,Male ,musculoskeletal diseases ,Adolescent ,Drug Resistance ,Apoptosis ,CHO Cells ,Dermatology ,Biology ,Pharmacology ,Transfection ,Biochemistry ,Jurkat cells ,Small hairpin RNA ,Jurkat Cells ,Young Adult ,Cricetulus ,Immune system ,Downregulation and upregulation ,immune system diseases ,Cricetinae ,ATP Binding Cassette Transporter, Subfamily G, Member 2 ,Animals ,Humans ,Psoriasis ,Protein Interaction Domains and Motifs ,Child ,skin and connective tissue diseases ,Molecular Biology ,Aged ,Dose-Response Relationship, Drug ,Chinese hamster ovary cell ,Middle Aged ,Neoplasm Proteins ,Up-Regulation ,HEK293 Cells ,Methotrexate ,Cell culture ,Case-Control Studies ,Basigin ,Leukocytes, Mononuclear ,ATP-Binding Cassette Transporters ,Female ,RNA Interference ,Dermatologic Agents ,Protein Binding - Abstract
Background Methotrexate (MTX) is a drug used to treat psoriasis due to inducing immune cell apoptosis. However, certain patients show MTX resistant. CD147, highly expressed by psoriatic PBMCs, is assumed to regulate MTX sensitivity. The underlining mechanism is still relatively understudied. Objective To understand the mechanisms of that CD147 promotes MTX resistance in immune cells. Methods The expression of CD147 and ABCG2 in PBMCs from psoriatic patients, cellular apoptosis and intracellular MTX amount were measured. We also checked the cellular drug sensitivity of CHO (Chinese Hamster Ovary) cell lines with introduced CD147 and Jurkat T cells depeleted CD147. By immunoprecipitation, we detected the interaction between CD147 and ABCG2. Results Both ABCG2 and CD147 are highly expressed in psoriatic PBMCs. Cultured in vitro, the PBMCs from psoriatic patients were more resistant to MTX-induced apoptosis comparing to PBMCs from healthy people. Further studies demonstrated that exogenous overexpression of CD147 in CHO cells increased ABCG2 protein level. After MTX treatment, CD147 overexpressing CHO cells showed lower apoptosis rate and lower intracellular MTX concentration. On the contrary, knockdown of CD147 by shRNA in Jurkat T cells decreased ABCG2 expression, as well as increased MTX-induced apoptosis and decreased MTX efflux. Immunoprecipitation experiment revealed that the trans-membrane domain of CD147 conferred its’ interaction with ABCG2. Conclusion Our study suggests a role of CD147 in regulating ABCG2 transportation of MTX in immune cells. Strategies involving targeting CD147 could be considered in clinical treatment of psoriatic patients resistant to MTX.
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- 2013
8. Application of high-frequency electrosurgical scalpel and methylene blue staining in endonasal dacryocystorhinostomy
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Zhongling, Luo, Lixin, Zhang, Yueguang, Qiu, and Xiaofang, Luo
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Adult ,Male ,Staining and Labeling ,Cautery ,Electrosurgery ,Endoscopy ,Middle Aged ,Surgical Flaps ,Methylene Blue ,Nasal Mucosa ,Lacrimal Duct Obstruction ,Humans ,Female ,Intraoperative Complications ,Dacryocystorhinostomy ,Nasolacrimal Duct ,Retrospective Studies - Abstract
To evaluate the application of a high-frequency electrosurgical scalpel and methylene blue staining in the endonasal dacryocystorhinostomy.This retrospective study included 37 patients (43 eyes) undergoing endonasal dacryocystorhinostomy in our hospital between 2011 and 2013 using methylene blue staining of the lacrimal sac and a high-frequency electrosurgical scalpel for cutting nasal mucosa, intraoperative stanch, and fixation of lacrimal sac and nasal mucosal flaps. Surgical efficacy, intraoperative challenges, and corresponding handling methods were evaluated and summarized.Among 43 eyes, 42 were successfully cured (97.7%) and the symptoms in 1 eye were improved (2.3%). Total efficacy rate was 100%. All surgeries were successfully performed. No severe intraoperative complications were observed.A high-frequency electrosurgical scalpel, combined with methylene blue staining of the lacrimal sac, is efficacious for nasal mucosal cutting, intraoperative stanch, and fixation of mucosal flap by cauterization, which significantly alleviates intraoperative complications and enhances surgical success rate. It deserves widespread application in clinical practice.
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- 2015
9. Design and baseline characteristics of a population-based study of eye disease in southern Chinese people: the Dongguan Eye Study
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Qianli, Meng, Ying, Cui, Min, Zhang, Lixin, Zhang, Liang, Zhang, Jinxin, Zhang, Jian, Kuang, Qingyang, Liu, Yang, Zheng, Zhongling, Luo, Huikun, Liu, Guoping, Zhu, Jingjing, Cai, Guanrong, Zhang, Xingxuan, Wen, and Haike, Guo
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Adult ,Aged, 80 and over ,Male ,Rural Population ,China ,Eye Diseases ,Health Status ,Visual Acuity ,Glucose Tolerance Test ,Middle Aged ,Blindness ,Lipids ,Cross-Sectional Studies ,Asian People ,Social Class ,Research Design ,Risk Factors ,Prevalence ,Quality of Life ,Body Constitution ,Humans ,Female ,Intraocular Pressure ,Visually Impaired Persons ,Aged - Abstract
To describe the study design, methodology and baseline characteristics of the Dongguan Eye Study.Population-based, cross-sectional studyA total of 8952 rural-dwelling residents aged 40 years or older in Hengli, Dongguan.The Dongguan Eye Study was conducted from September 2011 to February 2012. The interview covered demographic data, socio-economic status and health- and vision-related quality of life. Physical measurements included height, weight, waist and hip circumference, heart rate and blood pressure. Laboratory tests included fasting blood glucose, haemoglobin A1c, oral glucose tolerance, serum total cholesterol, triglycerides, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, serum creatinine, blood urea nitrogen and uric acid. Ophthalmic examinations included visual acuity and autorefraction testing, intraocular pressure measurement, slit-lamp examination, ocular biometry, gonioscopy, fundus photography, retinal nerve fibre layer imaging and visual field testing.Prevalence and risk factors for visual impairment, blindness, eye diseases and their associations with systemic medical indicators or health-related lifestyles, as well as epidemiological data on diabetic subjects. Methodology, response rates and baseline characteristics are presented.Of the 11 357 individuals eligible for the Dongguan Eye Study, 8952 (78.82%) subjects participated. All participants were self-identified Han Chinese. The average age was 54.0 years, 59.9% were female, 48.4% were farmers and 77.2% had elementary or junior middle school educational levels. The average body mass index and waist-hip ratio were 24.6 ± 3.9 kg/m(2) and 0.9 ± 0.2.Data from the Dongguan Eye Study provide information concerning the prevalence, risk factors and impacts of eye diseases in rural residents undergoing urbanization in southern China.
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- 2015
10. A novel interaction between calcium-modulating cyclophilin ligand and Basigin regulates calcium signaling and matrix metalloproteinase activities in human melanoma cells
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Weiqi Zeng, Zhongling Luo, Jianglin Zhang, Zhao-Qian Liu, Juan Su, Tingting Long, Hong-Hao Zhou, Wen Tang, Xiang Chen, Min Qi, and Shuang Liu
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Cancer Research ,SERCA ,Thapsigargin ,Intracellular Space ,Biology ,chemistry.chemical_compound ,Humans ,Protein Interaction Domains and Motifs ,Calcium Signaling ,Melanoma ,Calcium signaling ,Adaptor Proteins, Signal Transducing ,Endoplasmic reticulum ,Transmembrane activator and CAML interactor ,Matrix Metalloproteinases ,Gene Expression Regulation, Neoplastic ,Protein Transport ,Oncology ,chemistry ,Matrix Metalloproteinase 9 ,Basigin ,Second messenger system ,Cancer research ,Calcium ,Signal transduction ,Protein Binding - Abstract
Intracellular free calcium is a ubiquitous second messenger regulating a multitude of normal and pathogenic cellular responses, including the development of melanoma. Upstream signaling pathways regulating the intracellular free calcium concentration ([Ca2+]i) may therefore have a significant impact on melanoma growth and metastasis. In this study, we demonstrate that the endoplasmic reticulum (ER)-associated protein calcium-modulating cyclophilin ligand (CAML) is bound to Basigin, a widely expressed integral plasma membrane glycoprotein and extracellular matrix metalloproteinase inducer (EMMPRIN, or CD147) implicated in melanoma proliferation, invasiveness, and metastasis. This interaction between CAML and Basigin was first identified using yeast two-hybrid screening and further confirmed by co-immunoprecipitation. In human A375 melanoma cells, CAML and Basigin were co-localized to the ER. Knockdown of Basigin in melanoma cells by siRNA significantly decreased resting [Ca2+]i and the [Ca2+]i increase induced by the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) inhibitor thapsigargin (TG), indicating that the interaction between CAML and Basigin regulates ER-dependent [Ca2+]i signaling. Meanwhile upregulating the [Ca2+]i either by TG or phorbol myristate acetate (PMA) could stimulate the production of MMP-9 in A375 cells with the expression of Basigin. Our study has revealed a previously uncharacterized [Ca2+]i signaling pathway that may control melanoma invasion, and metastasis. Disruption of this pathway may be a novel therapeutic strategy for melanoma treatment.
- Published
- 2013
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