1. 8-bromo-cyclicAMP stimulates glucose transporter-1 expression in a human choriocarcinoma cell line
- Author
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Masahiro Sakata, Hirohisa Kurachi, Y. Yasui, Masaya Yamaguchi, Yuji Murata, K. Ogura, T Maeda, Yasuhiro Yoshimoto, Yoko Okamoto, and C Tadokoro
- Subjects
Serotonin ,endocrine system ,medicine.medical_specialty ,Monosaccharide Transport Proteins ,Endocrinology, Diabetes and Metabolism ,Glucose uptake ,8-Bromo Cyclic Adenosine Monophosphate ,Biology ,Endocrinology ,Syncytiotrophoblast ,Leucine ,Pregnancy ,Internal medicine ,Tumor Cells, Cultured ,medicine ,Humans ,Choriocarcinoma ,reproductive and urinary physiology ,Glucose Transporter Type 1 ,Cytotrophoblast ,Dose-Response Relationship, Drug ,Glucose transporter ,Trophoblast ,Immunohistochemistry ,medicine.anatomical_structure ,Uterine Neoplasms ,embryonic structures ,biology.protein ,Female ,GLUT1 ,GLUT3 - Abstract
Facilitative glucose transporter-1 (GLUT1) is abundant in trophoblast cells and is responsible for glucose transport in the placenta. However, the change in GLUT expression in human placenta upon trophoblast differentiation remains to be clarified. Therefore, we first examined the localization of GLUT1 and GLUT3 using human first-trimester chorionic villi. We found that GLUT1 and GLUT3 were mainly localized to syncytiotrophoblast and cytotrophoblast cells respectively. We analyzed whether placental GLUT1 and GLUT3 expression changes during differentiation using a human choriocarcinoma (BeWo) cell line which is known to show functional and morphological differentiation in response to cAMP in culture. Treatment of BeWo cells with 8-bromo-cyclicAMP (8-bromo-cAMP) increased the level of hCG secretion and induced cell fusion leading to the formation of large syncytia. Treatment of BeWo cells with 8-bromo-cAMP also resulted in a significant increase in glucose uptake on days 2-3 of culture. The stimulating effect of 8-bromo-cAMP on glucose uptake was concentration dependent. Northern and immunoblot analyses revealed that the levels of mRNA and protein of GLUT1, but not of GLUT3, were significantly increased by 8-bromo-cAMP. These findings suggest that 8-bromo-cAMP stimulates GLUT1 expression with differentiation in BeWo cells.
- Published
- 2000