19 results on '"Tao, Cong"'
Search Results
2. Evaluation of left atrial function and mechanical dispersion in breast cancer patients after chemotherapy
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Na Chen, Ansheng Liu, Siyao Sun, Hong Wei, Qiaobing Sun, Zhijuan Shang, Yinghui Sun, Tingting Fu, Hongjiang Wang, Yunlong Xia, Lanqi Hua, David H. Hsi, and Tao Cong
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Echocardiography ,Heart Ventricles ,Humans ,Atrial Function, Left ,Breast Neoplasms ,Female ,Heart Atria ,General Medicine ,Cardiology and Cardiovascular Medicine ,Retrospective Studies - Abstract
Left atrial (LA) function and mechanical dispersion changes in breast cancer patients treated with chemotherapy remain unclear.LA function and LA mechanical dispersion in breast cancer patients would be impaired after chemotherapy.This single-center retrospective study included 91 consecutive breast cancer patients treated with chemotherapy and 30 controls. Patients were examined by echocardiography three times at intervals. Conventional parameters, left ventricular strain, LA strain, and LA mechanical dispersion were evaluated and compared.LA strain during reservoir phase (LASr), conduit phase (LAScd), and contraction phase (LASct) all decreased markedly after chemotherapy and were lower than those of the controls (all p .01). The standard deviation of time to peak positive strain during LA reservoir phase corrected by R-R interval (LA SD-TPSr) was significantly increased after chemotherapy and was higher than that of the controls (p .001). The change of LA function was expressed as Δ. Multivariate linear regression analyses showed that LAVIp (0.399, 95% confidence interval [CI]: 0.610, 1.756, p = .000) was independently associated with ΔLASr, LAPEF (-0.325, 95% CI: -45.123, -10.676, p = .002) and age (0.227, 95% CI: 0.021, 0.350, p = .027) were independently associated with ΔLAScd, and LAVImax (0.341, 95% CI: 0.192, 0.723, p = .001) was independently associated with ΔLASct. LAVImax (0.505, 95% CI: 0.000, 0.001, p = .039) and mitral E (-0.256, 95% CI: 0.000, 0.000, p = .024)were independently associated with ΔLA SD-TPSr.Mechanical function of LA declined after chemotherapy in breast cancer patients. With the decrease of LA mechanical function, LA mechanical dispersion assessed by two-dimensional speckle-tracking echocardiography increased significantly, and its clinical value needs to be further studied.
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- 2022
3. Effects of blood pressure and heart rate circadian rhythms on left atrial function
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Ying Zhang, Yixiao Zhao, Yan Lu, Qiaobing Sun, Yunpeng Cheng, Jiayu Han, Yan Liu, Yushan Wei, Wei Song, Yinong Jiang, and Tao Cong
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medicine.medical_specialty ,Physiology ,business.industry ,Stiffness index ,Blood Pressure ,Speckle tracking echocardiography ,Blood Pressure Monitoring, Ambulatory ,Nocturnal ,Circadian Rhythm ,Blood pressure ,Heart Rate ,Left atrial ,Internal medicine ,Hypertension ,Heart rate ,Ambulatory ,Internal Medicine ,medicine ,Cardiology ,Humans ,Atrial Function, Left ,cardiovascular diseases ,Circadian rhythm ,Cardiology and Cardiovascular Medicine ,business - Abstract
Objective We examined the associations among the circadian rhythms of blood pressure (BP), heart rate (HR) and left atrial function in essential hypertensive patients. Methods The study included 237 essential hypertensive patients who completed 24-h ambulatory BP, HR monitoring and two-dimensional speckle tracking echocardiography (2DSTE). The strain and strain rate images were studied, and the following parameters were measured: left atrial reservoir strain and strain rate (LAS-S and LASR-S), left atrial conduit strain and strain rate (LAS-E and LASR-E), and left atrial booster strain and strain rate (LAS-A and LASR-A). The left atrial stiffness index (LASI) was identified as the ratio of E/e' to LAS-S. All participants were divided into three groups according to the percentage of nocturnal BP dipping (dippers, nondippers and reverse dippers). Results The LASI was significantly higher in BP reverse dippers than in dippers and nondippers. LAS-S, LAS-E and LASR-E were significantly lower in BP reverse dippers than dippers and nondippers. Multivariate logistic regression analysis demonstrated that age, night-time mean SBP and the percentage of nocturnal HR decline were independently related to an increased LASI. Conclusion Impairment of the left atrial reservoir and conduit functions was correlated with abnormal BP and HR circadian rhythms in hypertension. Increased left atrial stiffness was associated with night-time SBP and the percentage of nocturnal HR decline.
- Published
- 2021
4. Time interval between alfentanil and rocuronium administration necessary to prevent rocuronium-induced withdrawal movement
- Author
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Xiao-Dan Wang, Ling-yang Chen, Chun-Lian Zhou, Hai-tao Cong, Nan-jin Chen, and Ming-Cang Wang
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Adult ,Male ,Research ,Movement ,Pain ,Time ,Analgesics, Opioid ,Anesthesiology and Pain Medicine ,Sex Factors ,Double-Blind Method ,Anesthesiology ,Heart Rate ,Humans ,RD78.3-87.3 ,Arterial Pressure ,Female ,Prospective Studies ,Alfentanil ,Rocuronium ,Pain-induced withdrawal movement ,Neuromuscular Nondepolarizing Agents - Abstract
Background We aimed to determine the time interval between alfentanil and rocuronium administration, at a 50% probability of preventing pain-induced withdrawal movement from rocuronium injection (TimeAR50). Methods A total of 64 patients scheduled for general anesthesia were enrolled in this study (33 men and 31 women). Anesthesia was induced with target-controlled infusion of propofol, at an effect-site target concentration of 3 μg/mL. Then, alfentanil 15 μg/kg was injected for 30 s. After 60 s, rocuronium 0.6 mg/kg was administered to the first patient. The Dixon’s up-and-down method was used to determine the time interval for each subsequent patient (interval of 5 s). Mean arterial pressure (MAP) and heart rate (HR) were recorded at three time points: T0, pre-induction; T1, before rocuronium injection; and T2, 1 min after rocuronium injection. Results The TimeAR50 ± standard deviation (SD) was 5.6 ± 3.7 s and 21.9 ± 5.6 s in the male and female patients, respectively. Based on the probit regression, the TimeAR50 was 4.7 s (95% confidence interval [CI], 1.2–7.6 s) and 20.3 s (95% CI, 7.7–26.1 s) in the male and female patients, respectively. The TimeAR95 was 10.6 s (95% CI, 7.7–25.3 s) and 35.0 s (95% CI, 28.1–95.5 s) in the male and female patients, respectively, with significantly higher values in females than in males (P Conclusion The TimeAR50 required for preventing rocuronium-induced withdrawal movement were 4.7 s and 20.3 s in male and female patients, respectively. Trial registration This study was registered with the Chinese Clinical Trials Registry on April 7, 2021 (URL: http://www.chictr.org.cn. Registry number: ChiCTR2100045137) .
- Published
- 2022
5. Percutaneous closure of the left atrial appendage: The value of real time 3D transesophageal echocardiography and the intraoperative change in the size of the left atrial appendage
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Lizi Zhang, Tao Cong, and Ansheng Liu
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Male ,medicine.medical_specialty ,Percutaneous ,Septal Occluder Device ,Echocardiography, Three-Dimensional ,030204 cardiovascular system & hematology ,Severity of Illness Index ,03 medical and health sciences ,0302 clinical medicine ,Left atrial ,Internal medicine ,Preoperative Care ,medicine ,Humans ,Atrial Appendage ,Radiology, Nuclear Medicine and imaging ,030212 general & internal medicine ,Aged ,Retrospective Studies ,Appendage ,Intraoperative Care ,business.industry ,Reproducibility of Results ,Landing zone ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,Echocardiography, Transesophageal - Abstract
OBJECTIVE The aim of this study was to investigate the value of real time three-dimensional transesophageal echocardiography (RT3DTEE) in percutaneous closure of the left atrial appendage (LAAC). In addition, this study also explored the change in the size of the left atrial appendage (LAA) from 24 hours before the operation to just before implantation during the operation. METHODS AND RESULTS In a retrospective study, 32 patients underwent two-dimensional transesophageal echocardiography (2DTEE) and RT3DTEE 24 hours prior to operation and during operation. The maximal LAA orifice diameter (by 2DTEE, 22.7 ± 2.7 vs 24.6 ± 2.2 mm, P
- Published
- 2019
6. Comparison of cryoballoon ablation for atrial fibrillation guided by real-time three-dimensional transesophageal echocardiography vs. contrast agent injection
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Yuan-Jun Sun, Xiao-Meng Yin, Tao Cong, Lian-Jun Gao, Dong Chang, Xian-Jie Xiao, Qiao-Bing Sun, Rong-Feng Zhang, Xiao-Hong Yu, Ying-Xue Dong, Yan-Zong Yang, Yun-Long Xia, and Yi Cui
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Male ,medicine.medical_specialty ,media_common.quotation_subject ,medicine.medical_treatment ,Echocardiography, Three-Dimensional ,Contrast Media ,lcsh:Medicine ,Pulmonary veins ,Balloon ,Cryosurgery ,Pulmonary vein ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Occlusion ,medicine ,Humans ,Fluoroscopy ,Contrast (vision) ,Transesophageal echocardiography ,Aged ,media_common ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Atrial fibrillation ,Cryoablation ,Original Articles ,General Medicine ,Middle Aged ,medicine.disease ,Ablation ,Treatment Outcome ,Cryoballoon ablation ,Balloon occlusion ,030220 oncology & carcinogenesis ,Cardiology ,Female ,business ,human activities ,Echocardiography, Transesophageal ,030217 neurology & neurosurgery - Abstract
Background: Pulmonary vein (PV) occlusion generally depends on repetitive contrast agent injection when cryoballoon ablation for atrial fibrillation (AF). The present study was to compare the effect of cryoballoon ablation for AF guided by transesophageal echocardiography (TEE) vs. contrast agent injection. Methods: Eighty patients with paroxysmal AF (PAF) were enrolled in the study. About 40 patients underwent cryoballoon ablation without TEE (non-TEE group) and the other 40 underwent cryoballoon ablation with TEE for PV occlusion (TEE group). In the TEE group during the procedure, PVs were displayed in 3-dimensional images to guide the balloon to achieve PV occlusion. The patients were followed up at regularly scheduled visits every 2 months. Results: No differences were identified between the groups in regard to the procedure time and cryoablation time for each PV. The fluoroscopy time (6.7±4.2 min vs. 17.9±5.9 min, P
- Published
- 2019
7. Application of Rough Ant Colony Algorithm in Adolescent Psychology
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Lin Jiang, Tao Cong, Qihang Sun, and Yang Li
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General Computer Science ,Article Subject ,Adolescent ,Computer science ,020209 energy ,General Mathematics ,Big data ,Computer applications to medicine. Medical informatics ,Psychology, Adolescent ,R858-859.7 ,Neurosciences. Biological psychiatry. Neuropsychiatry ,02 engineering and technology ,Pheromones ,Domain (software engineering) ,0202 electrical engineering, electronic engineering, information engineering ,Juvenile delinquency ,Humans ,Degree (graph theory) ,business.industry ,General Neuroscience ,Ant colony optimization algorithms ,General Medicine ,Ant colony ,020201 artificial intelligence & image processing ,Artificial intelligence ,Rough set ,business ,Algorithms ,Premature convergence ,RC321-571 ,Research Article - Abstract
With the rapid development of big data, big data research in the security protection industry has been increasingly regarded as a hot spot. This article mainly aims at solving the problem of predicting the tendency of juvenile delinquency based on the experimental data of juvenile blindly following psychological crime. To solve this problem, this paper proposes a rough ant colony classification algorithm, referred to as RoughAC, which first uses the concept of upper and lower approximate sets in rough sets to determine the degree of membership. In addition, in the ant colony algorithm, we use the membership value to update the pheromone. Experiments show that the algorithm can not only solve the premature convergence problem caused by stagnation near the local optimal solution but also solve the continuous domain and combinatorial optimization problems and achieve better classification results. Moreover, the algorithm has a good effect on predicting classification and can provide guidance for predicting the tendency of juvenile delinquency.
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- 2020
8. Left atrial stiffness index as a marker of early target organ damage in hypertension
- Author
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Yan Liu, Yinong Jiang, Wei Song, Yixiao Zhao, Qiaobing Sun, Yan Lu, Yunpeng Cheng, Jiayu Han, Tao Cong, and Ying Zhang
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medicine.medical_specialty ,Physiology ,Group ii ,Cardiovascular risk factors ,Stiffness index ,030204 cardiovascular system & hematology ,Left ventricular hypertrophy ,Essential hypertension ,03 medical and health sciences ,0302 clinical medicine ,Vascular Stiffness ,Left atrial ,Internal medicine ,Internal Medicine ,Medicine ,Humans ,030212 general & internal medicine ,business.industry ,medicine.disease ,Target organ damage ,Echocardiography ,Case-Control Studies ,Hypertension ,Cardiology ,Atrial Function, Left ,Hypertrophy, Left Ventricular ,Gradual increase ,Cardiology and Cardiovascular Medicine ,business - Abstract
This study aimed to evaluate left atrial (LA) mechanics using two-dimensional speckle-tracking echocardiography (2DSTE) and investigate their correlations with measures of target organ damage (TOD) in hypertension. We enrolled 42 healthy controls (Group I) and 286 hypertension patients: Group II (n = 79) had an LA volume index (LAVI)
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- 2020
9. Accuracy of cardiac CT in evaluating severity of left atrial appendage spontaneous echo contrast: comparison with transesophageal echocardiography
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Xi-Xia Sun, Si-Yao Sun, Chongfu Jia, Zhi-qiang Yang, Liang Zhang, Chun-Li He, Tao Cong, and Zhao-Qian Wang
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Adult ,Male ,Heart Diseases ,Spontaneous echo contrast ,Contrast Media ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Left atrial ,medicine.artery ,Hounsfield scale ,Atrial Fibrillation ,Ascending aorta ,medicine ,Humans ,Cutoff ,Atrial Appendage ,Radiology, Nuclear Medicine and imaging ,Cardiac imaging ,Aged ,Retrospective Studies ,Aged, 80 and over ,Receiver operating characteristic ,business.industry ,Thrombosis ,Atrial fibrillation ,Middle Aged ,medicine.disease ,Cardiac Imaging Techniques ,Female ,Tomography, X-Ray Computed ,Cardiology and Cardiovascular Medicine ,business ,Nuclear medicine ,Echocardiography, Transesophageal ,030217 neurology & neurosurgery - Abstract
To investigate the capacity of biphasic cardiac CT (CCT) for qualitative and quantitative evaluation of different grades of left atrial appendage spontaneous echo contrast (LAASEC). The study included 267 inpatients with confirmed atrial fibrillation who underwent both CCT and transesophageal echocardiography (TEE). CT numbers for LAA, ascending aorta (AA), and left atrium (LA) were identified, and ROC curves for LAA, LAA/AA, and LAA/LA were plotted. With TEE as the standard, the sensitivity, specificity, PPV, NPV, and accuracy of CCT for LAASEC grade ≥ 1 were 60.3, 92.9, 92.4, 64.8, and 75.7%; and for grade ≥ 2 were 100.0, 84.4, 71.4, 100.0, and 88.8% respectively. The values of LAA, LAA/AA, and LAA/LA were significantly larger in LAASEC grade 0 versus 1 and in grade 1 versus 2, but were similar in grades 2 and 3 or in grades 3 and 4. The values of LAA/AA were larger in grade 2 versus 4. When the cutoff value for LAA/AA = 0.897, sensitivity, specificity, PPV, NPV, and accuracy of CCT for LAASEC grade ≥ 1 was 89.6, 83.2, 87.9, 85.5, and 86.9% and with a cutoff for LAA/AA of 0.524, the sensitivity, specificity, PPV, NPV, and accuracy for LAASEC grade ≥ 2 was 98.7, 92.7, 84.1, 99.4, and 94.4%. Although CCT showed limited diagnostic accuracy for grade 1 LAASEC, grade ≥ 2 LAASEC could be excluded when there was no LAA filling defect on first-phase CCT, and TEE can be avoided. CCT has an excellent accuracy in diagnosing LAASEC, and quantitative analysis (in particular LAA/AA) is superior.
- Published
- 2018
10. Prognostic Value of Speckle Tracking Echocardiography in Patients with ST-Elevation Myocardial Infarction Treated with Late Percutaneous Intervention
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Ke Wang, Tao Cong, Yanzong Yang, Lei Zhong, Zhijuan Shang, Shulong Zhang, Dechun Su, and Yinghui Sun
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Heart Ventricles ,medicine.medical_treatment ,Myocardial Infarction ,Infarction ,Speckle tracking echocardiography ,Sensitivity and Specificity ,Young Adult ,Percutaneous Coronary Intervention ,Internal medicine ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,Myocardial infarction ,Ventricular remodeling ,Aged ,Ultrasonography ,Aged, 80 and over ,Ejection fraction ,Ventricular Remodeling ,business.industry ,Percutaneous coronary intervention ,Middle Aged ,Prognosis ,medicine.disease ,Heart failure ,Conventional PCI ,Cardiology ,Female ,Cardiology and Cardiovascular Medicine ,business ,Follow-Up Studies - Abstract
Background Left ventricular remodeling (LVr) is common after ST-segment elevation myocardial infarction (STEMI). The aim of this study was to evaluate the prognostic value of speckle tracking echocardiography (STE) for predicting LVr 6–9 months after late percutaneous coronary intervention (PCI) in patients with STEMI. Methods Patients with first STEMI who accepted late PCI were enrolled. Echocardiography was performed within 48 hours of admission. Six to nine months after MI, an echocardiography examination was repeated. LVr was defined as >15% increase in LV end-systolic volume (LVESV) after 6 months. Results One hundred and twenty-seven patients were divided into two groups: 86 patients without LVr and 41 patients with LVr. There were significant differences in the global longitudinal strain (GLS), SD of time to peak longitudinal systolic strain (longitudinal Ts-SD), longitudinal postsystolic index, radial strain (RS), and SD of time to peak radial systolic strain (Radial Ts-SD). In multivariate logistic regression analysis, the GLS(odds ratio [OR] = 0.39, 95% confidence interval [CI] = 0.26–0.57, P
- Published
- 2014
11. Assessment of left atrial mechanical function and synchrony in paroxysmal atrial fibrillation with two-dimensional speckle tracking echocardiography
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Tao Cong, Yan Liu, Dechun Su, Yinghui Sun, Jun Yang, Na Chen, and Zhijuan Shang
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Male ,medicine.medical_specialty ,Longitudinal strain ,Paroxysmal atrial fibrillation ,Diastole ,Speckle tracking echocardiography ,030204 cardiovascular system & hematology ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,Left atrial ,Internal medicine ,Atrial Fibrillation ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,030212 general & internal medicine ,Heart Atria ,Cardiac cycle ,business.industry ,Reproducibility of Results ,Atrial fibrillation ,respiratory system ,Middle Aged ,medicine.disease ,Echocardiography ,Cardiology ,Time to peak ,Atrial Function, Left ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Background The aim of this study was to investigate left atrial (LA) function and synchrony in paroxysmal atrial fibrillation (PAF) patients using two-dimensional speckle tracking echocardiography (STE). Methods Forty-five PAF patients and 30 healthy controls were enrolled. LA peak ventricular systolic longitudinal strain (LAS-S) and strain rate (LASR-S) and left atrial longitudinal strain (LAS-A) and strain rate (LASR-A) during late diastole were determined using STE, and the standard deviation of the time to peak (TPSD) of the regional strains was calculated to quantify LA dyssynchrony. TPSD during ventricular systole and late diastole were named SDs and SDa, respectively. Results Left atrial peak longitudinal strain during ventricular systole (LAS-S) (29.34±8.57 vs 36.73±6.13), LASR-S (1.27±0.311 vs 1.57±0.25), LAS-A (13.11±4.91 vs 17.86±3.57), and LASR-A (−1.51±0.58 vs −1.90±0.30) were reduced in the PAF group compared with the controls (P
- Published
- 2017
12. Hedgehog signaling contributes to basic fibroblast growth factor-regulated fibroblast migration
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Ying Wang, Li Tai Jin, Wei Tao Cong, Jia Yong Zheng, Xuan Zhou, Yu Ting Zhu, Congcong Sun, Xiao Kun Li, Zhong Xin Zhu, Li Sha Chi, Tao Wang, and Wan Hui Cai
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0301 basic medicine ,integumentary system ,Fibroblast growth factor receptor 2 ,Basic fibroblast growth factor ,Cell Biology ,Fibroblast growth factor receptor 4 ,Fibroblast growth factor receptor 3 ,Biology ,Fibroblasts ,Fibroblast growth factor ,Hedgehog signaling pathway ,Fibroblast migration ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Cell Movement ,Immunology ,Humans ,Fibroblast Growth Factor 2 ,Hedgehog Proteins ,Smoothened ,Cells, Cultured ,Signal Transduction - Abstract
Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. Through the RNA-seq analysis, we had identified that the hedgehog (Hh) canonical pathway genes including Smoothened (Smo) and Gli1, were regulated by bFGF. Further analysis revealed that activation of the Hh pathway via up-regulation of Smo promoted fibroblast migration, invasion, and skin wound healing, but which significantly reduced by GANT61, a selective antagonist of Gli1/Gli2. Western blot analyses and siRNA transfection assays demonstrated that Smo acted upstream of phosphoinositide 3-kinase (PI3K)-c-Jun N-terminal kinase (JNK)-β-catenin to promote cell migration. Moreover, RNA-seq and qRT-PCR analyses revealed that Hh pathway genes including Smo and Gli1 were under control of β-catenin, suggesting that β-catenin turn feedback activates Hh signaling. Taken together, our analyses identified a new bFGF-regulating mechanism by which Hh signaling regulates human fibroblast migration, and the data presented here opens a new avenue for the wound healing therapy.
- Published
- 2017
13. GSK-3 Phosphorylates δ-Catenin and Negatively Regulates Its Stability via Ubiquitination/Proteosome-mediated Proteolysis
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Jinhyung No, Hyunkyoung Ki, Ja-Hye Park, Ilhwan Yang, Qun Lu, Inho Kwon, Minsoo Oh, Jung-Kap Choi, Moon-Chang Baek, Kwonseop Kim, Wei-Tao Cong, Hangun Kim, and Sonja K. Bareiss
- Subjects
Proteasome Endopeptidase Complex ,Delta Catenin ,Proteolysis ,macromolecular substances ,Biology ,Biochemistry ,Rats, Sprague-Dawley ,Glycogen Synthase Kinase 3 ,Mice ,GSK-3 ,medicine ,Animals ,Humans ,Enzyme Inhibitors ,Phosphorylation ,Molecular Biology ,GSK3B ,Neurons ,Glycogen Synthase Kinase 3 beta ,medicine.diagnostic_test ,Ubiquitin ,Kinase ,Protein Synthesis, Post-Translational Modification, and Degradation ,Wild type ,Catenins ,Cell Biology ,Fibroblasts ,Phosphoproteins ,Molecular biology ,Rats ,Cell biology ,Proteasome ,Culture Media, Conditioned ,Catenin ,Cell Adhesion Molecules - Abstract
Delta-catenin was first identified because of its interaction with presenilin-1, and its aberrant expression has been reported in various human tumors and in patients with Cri-du-Chat syndrome, a form of mental retardation. However, the mechanism whereby delta-catenin is regulated in cells has not been fully elucidated. We investigated the possibility that glycogen-synthase kinase-3 (GSK-3) phosphorylates delta-catenin and thus affects its stability. Initially, we found that the level of delta-catenin was greater and the half-life of delta-catenin was longer in GSK-3beta(-/-) fibroblasts than those in GSK-3beta(+/+) fibroblasts. Furthermore, four different approaches designed to specifically inhibit GSK-3 activity, i.e. GSK-3-specific chemical inhibitors, Wnt-3a conditioned media, small interfering RNAs, and GSK-3alpha and -3beta kinase dead constructs, consistently showed that the levels of endogenous delta-catenin in CWR22Rv-1 prostate carcinoma cells and primary cortical neurons were increased by inhibiting GSK-3 activity. In addition, it was found that both GSK-3alpha and -3beta interact with and phosphorylate delta-catenin. The phosphorylation of DeltaC207-delta-catenin (lacking 207 C-terminal residues) and T1078A delta-catenin by GSK-3 was noticeably reduced compared with that of wild type delta-catenin, and the data from liquid chromatography-tandem mass spectrometry analyses suggest that the Thr(1078) residue of delta-catenin is one of the GSK-3 phosphorylation sites. Treatment with MG132 or ALLN, specific inhibitors of proteosome-dependent proteolysis, increased delta-catenin levels and caused an accumulation of ubiquitinated delta-catenin. It was also found that GSK-3 triggers the ubiquitination of delta-catenin. These results suggest that GSK-3 interacts with and phosphorylates delta-catenin and thereby negatively affects its stability by enabling its ubiquitination/proteosome-mediated proteolysis.
- Published
- 2009
14. Proteome response to ochratoxin A-induced apoptotic cell death in mouse hippocampal HT22 cells
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Yeojin Bang, Seung Jun Kwack, Jung-Kap Choi, Somy Yoon, Hyun Jin Choi, Chul Su Yoon, Wei-Tao Cong, Tae Seok Kang, Sang No Lee, and Kwang Youl Lee
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Programmed cell death ,Proteome ,DNA damage ,Tetrazolium Salts ,Apoptosis ,Biology ,Toxicology ,medicine.disease_cause ,Hippocampus ,Mice ,Neuroblastoma ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Viability assay ,Prohibitin ,Cell Line, Transformed ,Analysis of Variance ,Dose-Response Relationship, Drug ,L-Lactate Dehydrogenase ,General Neuroscience ,Neurodegeneration ,Environmental exposure ,Calcium Channel Blockers ,medicine.disease ,Ochratoxins ,Mitochondria ,Thiazoles ,Gene Expression Regulation ,Biochemistry ,Cell culture ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Reactive Oxygen Species ,Nucleophosmin ,Oxidative stress - Abstract
Mycotoxins are commonly encountered natural products, and are capable of poisoning animals or humans that inhale mold particles from mycotoxin-contaminated foods. Ochratoxin A (OTA) is produced by Aspergillu ochracus and Penicillium verrucosum, and is often found in cereals and agricultural products. Although previous studies have focused on the potent nephrotoxicity and renal carcinogenicity of OTA, more recent studies suggest that it accumulates in the brain and causes oxidative stress and DNA damage in various brain regions and neuronal populations. In the present study, we undertook to investigate the potential harm caused by environmental exposure to OTA in terms of its effects on neuronal cell viability and proteome profiles. OTA was found to significantly reduce the viabilities of human neuroblastoma SH-SY5Y and mouse hippocampal HT22 cells, as assessed by lactic dehydrogenase release into culture media. Generation of reactive oxygen species was detected in OTA-treated SH-SY5Y and HT22 cells, however, caspase activation and increase in p53 phosphorylation were only detected in HT22 cells, and the expressions of several proteins were found to be significantly altered after treating HT22 cells with OTA. Valosin containing protein, prolyl 4-hydroxylase, Atp5b protein, nucleophosmin 1, eukaryotic translation elongation factor 1 delta isoform, ornithine aminotransferase, prohibitin, and peroxiredoxin 6, which have been suggested to be implicated in the pathogenesis of neurodegenerative disorders, were up-regulated. Our findings suggest that coordinated regulations of molecular networks are involved in the OTA-induced cytotoxicity and that proteome response can be an indicative for neurodegeneration.
- Published
- 2009
15. Sensitive fluorescent staining for proteomic analysis of proteins in 1-D and 2-D SDS-PAGE and its comparison with SYPRO Ruby by PMF
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Jung-Kap Choi, Wei-Tao Cong, Litai Jin, and Sun-Young Hwang
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Proteomics ,Berberine Alkaloids ,Clinical Biochemistry ,Sensitivity and Specificity ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Cell Line, Tumor ,Organometallic Compounds ,Humans ,Polyacrylamide gel electrophoresis ,Fluorescent Dyes ,chemistry.chemical_classification ,Chromatography ,Chemistry ,Proteins ,Reproducibility of Results ,Sodium Dodecyl Sulfate ,Palmatine ,Fluorescence ,Staining ,Sypro Ruby ,Spectrometry, Fluorescence ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Fluorescent staining ,Electrophoresis, Polyacrylamide Gel ,Counterion - Abstract
A novel fluorescence-based method for protein staining on SDS polyacrylamide gel is described. In this method, proteins are stained using counterion (palmatine and SDS) staining solution, which is inexpensive, easy to perform, and does not involve a destaining step. Fixing and staining of proteins using the counterion protocol take less than an hour. As little as 2 ng of protein can be detected. Another interesting feature of the staining protocol described here is the compatibility with MALDI-TOF MS which shows a similar number of identification score and sequence coverage compared with those of SYPRO Ruby.
- Published
- 2008
16. Improved conditions for fluorescent staining of proteins with 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid in SDS-PAGE
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Jung-Kap Choi, Sun-Young Hwang, Litai Jin, and Wei-Tao Cong
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Chromatography ,Chemistry ,Clinical Biochemistry ,Proteins ,Reproducibility of Results ,Sensitivity and Specificity ,Biochemistry ,Fluorescence ,Stain ,Anilino Naphthalenesulfonates ,Cell Line ,Analytical Chemistry ,Staining ,Sypro Ruby ,Matrix-assisted laser desorption/ionization ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Linear Models ,Organometallic Compounds ,Fluorescent staining ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Electrophoresis, Polyacrylamide Gel ,Polyacrylamide gel electrophoresis ,Fluorescent Dyes - Abstract
A simple and sensitive fluorescent staining method for the detection of proteins in SDS-PAGE, namely IB (improved 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid) stain, is described. Non-covalent hydrophobic probe 4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid was applied as a fluorescent dye, which can bind to hydrophobic sites in proteins non-specifically. As low as 1 ng of protein band can be detected briefly by 30 min washing followed by 15 min staining without the aiding of stop or destaining step. The sensitivity of the new presented protocol is similar to that of SYPRO Ruby, which has been widely accepted in proteomic research. Comparative analysis of the MS compatibility of IB stain and SYPRO Ruby stain allowed us to address that IB stain is compatible with the downstream of protein identification by PMF.
- Published
- 2008
17. Different Combinations of Right Ventricular Longitudinal Velocity and Displacement Can Be Applied Effectively for Diagnosis of Right Heart Failure in Chinese Patients
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Ke Wang, Dechun Su, Zhijuan Shang, Tao Cong, Wanxiang Zheng, Yinghui Sun, and Yan Liu
- Subjects
Male ,China ,medicine.medical_specialty ,Heart Ventricles ,Ventricular Dysfunction, Right ,Right heart failure ,Internal medicine ,Healthy volunteers ,medicine ,Humans ,Cutoff ,Myocardial Performance Index ,Retrospective Studies ,Heart Failure ,Ventricular function ,Receiver operating characteristic ,business.industry ,Incidence ,Stroke Volume ,Middle Aged ,Echocardiography, Doppler, Color ,Surgery ,ROC Curve ,Fractional area change ,Ventricular Function, Right ,Cardiology ,Female ,Right Ventricular Free Wall ,Cardiology and Cardiovascular Medicine ,business ,Blood Flow Velocity ,Follow-Up Studies - Abstract
Background: The noninvasive evaluation of right ventricular function (RVF) in cardiovascular and pulmonary diseases remains a challenge. Internationally recommended echocardiographic parameters for the evaluation of RVF may not be applicable to China, because of the limited examination time per patient, the varying skill levels of ultrasonographers, inadequate allocation of professional specialties, and outdated instruments and equipment.Methods: Sixty-two patients admitted for right heart failure and 52 healthy volunteers were included in the analysis. The tricuspid annular plane systolic excursion (TAPSE), peak systolic velocity in the tricuspid annulus of the right ventricular free wall (S′), myocardial performance index, right ventricular fractional area change, and Doppler peak strains in the base and mid cavity were evaluated and compared between groups.Results: Compared with the control group, all RVF parameters in the right heart failure group showed significant deterioration (P < .001). However, only TAPSE and S′ were finally included in the discriminant equation. The diagnostic cutoff values derived from the receiver operating characteristic curve were Conclusion: TAPSE and S′ are more suitable than other parameters for the diagnosis of right heart failure in Chinese patients. Different combinations of TAPSE and S′ can be applied effectively for the diagnosis of right heart failure.
- Published
- 2016
18. [Effect of cardiopulmonary bypass on the expression of survivin in neutrophils]
- Author
-
Xue-Zheng, Lin, Ming, Gong, Cong-Bin, Peng, Chun-Lian, Zhou, Hai-Tao, Cong, Yu, Wang, Zhen-Dong, Song, and Guang-Tao, Lu
- Subjects
Adult ,Male ,Cardiopulmonary Bypass ,Neutrophils ,Case-Control Studies ,Survivin ,Humans ,Apoptosis ,Female ,Middle Aged ,Microtubule-Associated Proteins ,Inhibitor of Apoptosis Proteins - Abstract
To investigate the effects of cardiopulmonary bypass on neutrophils apoptosis and the expression of survivin.Ten patients who scheduled for cardiac surgery under cardiopulmonary bypass were recruited as study group and 10 healthy volunteers as control. Blood samples were obtained before operation, at the end of surgery, and at 24 hours postoperatively. Neutrophils were isolated by density gradient centrifugation and its apoptosis were evaluated by fluorescent microscope and flow cytometry. The expression of survivin protein was examined by Western blotting analysis. Expression level of survivin mRNA was detected by RT-PCR.The apoptotic rate of neutrophils decreased significantly at the end of surgery (P0.01), and was still lower at 24 hours postoperatively than before operation (P0.05). The expression ratios of survivin protein and mRNA were increased at the end of surgery (P0.01), and decreased gently at 24 hours postoperatively but was still higher than before operation (P0.05).Cardiopulmonary bypass could inhibit neutrophils apoptosis and increase the expression of survivin. The decrease of neutrophils apoptosis was correlated with high expression of survivin.
- Published
- 2010
19. High-Glucose Inhibits Human Fibroblast Cell Migration in Wound Healing via Repression of bFGF-Regulating JNK Phosphorylation
- Author
-
Wei Tao Cong, Wan Hui Cai, Xi Wang, Hong Bo Ye, Hai Shan Tian, Li Tai Jin, Tie Min Wei, Bin Bin Huang, Yuan Hu Xuan, Yu Ting Zhu, Li Sha Chi, Zhong Xin Zhu, and Yuan Meng Duan
- Subjects
Proteomics ,medicine.medical_specialty ,MAP Kinase Kinase 4 ,lcsh:Medicine ,Apoptosis ,RAC1 ,Global Health ,Biochemistry ,Diabetes Mellitus, Experimental ,Endocrinology ,Cell Movement ,Internal medicine ,Medicine and Health Sciences ,Animals ,Humans ,Medicine ,Public and Occupational Health ,Phosphorylation ,lcsh:Science ,Fibroblast ,Cells, Cultured ,Diabetic Endocrinology ,Skin repair ,Wound Healing ,Multidisciplinary ,business.industry ,Cell growth ,Fibroblast growth factor receptor 1 ,lcsh:R ,Biology and Life Sciences ,Cell migration ,Fibroblasts ,Rats ,Cell biology ,Glucose ,medicine.anatomical_structure ,lcsh:Q ,Fibroblast Growth Factor 2 ,business ,Wound healing ,Annexin A2 ,Research Article - Abstract
One of the major symptoms of diabetes mellitus (DM) is delayed wound healing, which affects large populations of patients worldwide. However, the underlying mechanism behind this illness remains elusive. Skin wound healing requires a series of coordinated processes, including fibroblast cell proliferation and migration. Here, we simulate DM by application of high glucose (HG) in human foreskin primary fibroblast cells to analyze the molecular mechanism of DM effects on wound healing. The results indicate that HG, at a concentration of 30 mM, delay cell migration, but not cell proliferation. bFGF is known to promote cell migration that partially rescues HG effects on cell migration. Molecular and cell biology studies demonstrated that HG enhanced ROS production and repressed JNK phosphorylation, but did not affect Rac1 activity. JNK and Rac1 activation were known to be important for bFGF regulated cell migration. To further confirm DM effects on skin repair, a type 1 diabetic rat model was established, and we observed the efficacy of bFGF on both normal and diabetic rat skin repair. Furthermore, proteomic studies identified an increase of Annexin A2 protein nitration in HG-stressed fibroblasts and the nitration was protected by activation of bFGF signaling. Treatment with FGFR1 and JNK inhibitors delayed cell migration and increased Annexin A2 nitration levels, indicating that Annexin A2 nitration is modulated by bFGF signaling via activation of JNK. Together with these results, our data suggests that the HG-mediated delay of cell migration is linked to the inhibition of bFGF signaling, specifically through JNK suppression.
- Published
- 2014
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