Author: "T, Sasaki" / Topic: humans - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"T, Sasaki"' showing total 1,430 results

Start Over Author "T, Sasaki" Topic humans

1,430 results on '"T, Sasaki"'

1. Beyond Warburg: LDHA activates RAC for tumour growth

Author: Natsuski, Osaka and Atsuo T, Sasaki
Subjects: Neoplasms, Humans, Lactate Dehydrogenase 5, Article
Abstract: The glycolytic enzyme lactate dehydrogenase A (LDHA) is frequently overexpressed in cancer, which promotes glycolysis and cancer. The oncogenic effect of LDHA has been attributed to its glycolytic enzyme activity. Here, we report an unexpected noncanonical oncogenic mechanism of LDHA; LDHA activates small GTPase Rac1 to promote cancer independently of its glycolytic enzyme activity. Mechanistically, LDHA interacts with the active form of Rac1, Rac1-GTP, to inhibit Rac1-GTP interaction with its negative regulator, GTPase-activating proteins, leading to Rac1 activation in cancer cells and mouse tissues. In clinical breast cancer specimens, LDHA overexpression is associated with higher Rac1 activity. Rac1 inhibition significantly suppresses the oncogenic effect of LDHA. Combination inhibition of LDHA enzyme activity and Rac1 activity by small-molecule inhibitors displays a synergistic inhibitory effect on breast cancers with LDHA overexpression. These results reveal a critical oncogenic mechanism of LDHA and suggest a promising therapeutic strategy for breast cancers with LDHA overexpression.
Published: 2022

2. Schlafen 11 predicts response to platinum-based chemotherapy in gastric cancers

Author: Daiki Taniyama, Takuya Hattori, Kenji Harada, Eiichi Morii, Shoichi Ukai, Hideki Ohdan, Ririno Honma, Atsuo T. Sasaki, Junko Murai, Tsuyoshi Takashima, Maika Yasumoto, Kazuya Kuraoka, Ryuichi Asai, Wataru Yasui, Naoya Sakamoto, Kazuaki Tanabe, Pham Quoc Thang, and Ryota Maruyama
Subjects: Drug, Cancer Research, Cell Survival, media_common.quotation_subject, medicine.medical_treatment, Down-Regulation, Drug resistance, Article, Epigenesis, Genetic, Gene Knockout Techniques, 03 medical and health sciences, 0302 clinical medicine, Stomach Neoplasms, Cell Line, Tumor, medicine, Humans, Epigenetics, Cell Proliferation, Platinum, media_common, Chemotherapy, Transition (genetics), business.industry, Nuclear Proteins, Prognosis, Survival Analysis, Oxaliplatin, Gene Expression Regulation, Neoplastic, Treatment Outcome, Oncology, Drug Resistance, Neoplasm, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Immunohistochemistry, business, medicine.drug
Abstract: BACKGROUND: Although unresectable or recurrent gastric cancers (GC) are frequently treated with platinum-based chemotherapy, response to treatment remains unpredictable. Because Schlafen 11 (SLFN11) is recently identified as a critical determinant of platinum sensitivity, we investigated the potential clinical utility of SLFN11 in the treatment of GC. METHODS: We analysed the correlation between SLFN11 expression and overall survival in 169 GC patients by our established immunohistochemical approach. The impact of SLFN11 expression on the response to platinum and transition of SLFN11 expression upon long-term treatment with platinum were examined using GC cell lines and organoids. RESULTS: GC patients with high-SLFN11 expression exhibited significantly better survival than those with low-SLFN11 expression, and the significance increased when we selected patients treated with platinum-based chemotherapy. Knockout of SLFN11 and reactivation of SLFN11 in GC cells conferred resistance and sensitivity to platinum, respectively. In GC cells and organoids, long-term treatment with oxaliplatin suppressed SLFN11 expression while imparting drug resistance. The acquired resistance to oxaliplatin was reversed by reactivation of SLFN11 with epigenetic modifying drugs. CONCLUSIONS: This is the first report revealing definitive clinical implications of SLFN11 in the treatment of GC patients and providing novel strategies for the drug selection based on SLFN11 expression.
Published: 2021
Full Text: View/download PDF

3. Clinical usefulness of a novel fluorescence technique for the intraoperative diagnosis of surgical margins in patients with breast cancer

Author: H Ueo, I Minoura, A Gamachi, T Doi, M Yamaguchi, T Yamashita, H Tsuda, T Moriya, R Yamaguchi, Y Kozuka, T Sasaki, T Masuda, Y Kai, Y Kubota, Y Urano, M Mori, and K Mimori
Subjects: medicine.medical_specialty, Intraoperative Care, Rhodamines, business.industry, MEDLINE, Margins of Excision, Breast Neoplasms, Mastectomy, Segmental, medicine.disease, Fluorescence, Breast cancer, Humans, Medicine, Female, Surgery, In patient, Radiology, business, Fluorescent Dyes
Abstract: In both 5- and 15-min data, FI was significantly higher in malignant tissues than in benign tissues. The diagnostic accuracy was similar at 5 and 15 min. Therefore, the 5-min FI was enough applying in the further analyses.
Published: 2021
Full Text: View/download PDF

4. Droplet-digital PCR reveals frequent mutations in TERT promoter region in breast fibroadenomas and phyllodes tumours, irrespective of the presence of MED12 mutations

Author: T. Sasaki, Yasuyuki Seto, Kazutaka Otsuji, and Masahiko Tanabe
Subjects: Adult, Cancer Research, Adolescent, Breast Neoplasms, Biology, medicine.disease_cause, Polymerase Chain Reaction, Article, MED12, Young Adult, 03 medical and health sciences, symbols.namesake, Breast cancer, 0302 clinical medicine, Phyllodes Tumor, Breast Fibroadenoma, Cancer genomics, medicine, Humans, Digital polymerase chain reaction, Telomerase reverse transcriptase, Promoter Regions, Genetic, Cancer genetics, Telomerase, Gene, Aged, Sanger sequencing, Mutation, Mediator Complex, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Middle Aged, Molecular biology, Oncology, Fibroadenoma, 030220 oncology & carcinogenesis, symbols, Female, Carcinogenesis
Abstract: Background Breast fibroadenoma (FA) and phyllodes tumour (PT) often have variations of gene mediator complex subunit 12 (MED12) and mutations in the telomerase reverse transcriptase promoter region (TERTp). TERTp mutation is usually tested by Sanger sequencing. In this study, we compared Sanger sequencing and droplet-digital PCR (ddPCR) to measure TERTp mutations in FA and PT samples. Methods FA and PT samples were collected from 82 patients who underwent surgery at our institution from 2005 to 2016. MED12 mutations for all cases and TERTp mutations for 17 tumours were detected by Sanger sequencing. ddPCR was performed to analyse TERTp mutation in all cases. Results A total of 75 samples were eligible for analysis. Sanger sequencing detected MED12 mutations in 19/44 FA (42%) and 21/31 PT (68%). Among 17 Sanger sequencing-tested samples, 2/17 (12%) were TERTp mutation-positive. In ddPCR analyses, a significantly greater percentage of PT (19/31, 61%) was TERTp mutation-positive than was FA (13/44, 30%; P = 0.0046). The mutation positivity of TERTp and MED12 did not correlate, in either FA or PT. Conclusions ddPCR was more sensitive for detecting TERTp mutation than Sanger sequencing, being able to elucidate tumorigenesis in FA and PT.
Published: 2020
Full Text: View/download PDF

5. Impact of near-infrared fluorescence imaging with indocyanine green on structural sequelae of anastomotic leakage after laparoscopic intersphincteric resection of malignant rectal tumors

Author: H, Hasegawa, Y, Tsukada, M, Wakabayashi, S, Nomura, T, Sasaki, Y, Nishizawa, K, Ikeda, N, Takeshita, K, Teramura, and M, Ito
Subjects: Cohort Studies, Indocyanine Green, Male, Rectal Neoplasms, Anastomosis, Surgical, Optical Imaging, Anal Canal, Humans, Anastomotic Leak, Female, Laparoscopy, Middle Aged, Retrospective Studies
Abstract: Recent studies have indicated the potential benefit of intraoperative near-infrared fluorescence imaging (NIR-FI) with indocyanine green in reducing early anastomotic leakage in colorectal surgery. Nonetheless, whether NIR-FI is effective in reducing structural sequelae of anastomotic leakage (SSAL) remains unclear. The aim of the present study was to investigate the impact of NIR-FI on SSAL after laparoscopic intersphincteric resection (ISR) of malignant rectal tumors.This study was a retrospective single-center cohort study. A total of 293 consecutive patients who underwent elective laparoscopic ISR from May 2010 to August 2017 were included. Patients were divided into 2 groups; those who underwent elective laparoscopic ISR with lymphadenectomy for malignant rectal tumors using NIR-F (NIR-FI group) and those who underwent elective laparoscopic ISR with lymphadenectomy for malignant rectal tumors without using NIR-FI (control group). Thirty were excluded from the analyses (13 died, 7 had pelvic recurrence, and 10 were lost to follow-up). The primary endpoint was the rate of SSAL within 2 years after the primary resection, whereas the secondary endpoint was the rate of natural defecation via the anus at 2 years after the primary resection. Using various statistical analyses, such as propensity score matching, the rate of SSAL was compared between groups.A total of 263 patients were analyzed [177 males and 86 females, median age 61 (27-84) years]. Prior to propensity score matching (n = 263), NIR-FI was performed in 70 patients (26.6%) The rates of SSAL were 1.4% (1/70) in the NIR-FI group and 10.4% (20/193) in the control group (p = 0.02). After propensity score matching (n = 163), the rates of SSAL were 1.5% (1/66) in the NIR-FI group and 11.7% (12/103) in the control group (p = 0.02). Propensity score analyses, as well as simple regression analyses, revealed that NIR-FI was associated with a significantly lower risk of SSAL (OR 0.10-0.13; p = 0.03-0.05).NIR-FI is useful in reducing the rate of SSAL after laparoscopic ISR.
Published: 2021

6. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma

Author: Atsuo T. Sasaki, Kara Wolfe, Satoshi Kofuji, Hirofumi Yoshino, Mika Sasaki, and Koichi Okumura
Subjects: 0301 basic medicine, Guanylate kinase, Biophysics, Mitochondrion, Biochemistry, Isozyme, Article, 03 medical and health sciences, IMP Dehydrogenase, 0302 clinical medicine, Cell Line, Tumor, GMP synthase, Humans, Carbon-Nitrogen Ligases, Nucleotide, Purine metabolism, Carcinoma, Renal Cell, Purine Nucleotides, Molecular Biology, chemistry.chemical_classification, Cell Biology, NM23 Nucleoside Diphosphate Kinases, Kidney Neoplasms, Cell biology, 030104 developmental biology, chemistry, Cytoplasm, 030220 oncology & carcinogenesis, Guanylate Kinases, Intracellular
Abstract: Compartmentalization is vital for biological systems at multiple levels, including biochemical reactions in metabolism. Organelle-based compartments such as mitochondria and peroxisomes sequester the responsible enzymes and increase the efficiency of metabolism while simultaneously protecting the cell from dangerous intermediates, such as radical oxygen species. Recent studies show intracellular nucleotides, such as ATP and GTP, are heterogeneously distributed in cells with high concentrations at the lamellipodial and filopodial projections, or leading edge. However, the intracellular distribution of purine nucleotide enzymes remains unclear. Here, we report the enhanced localization of GTP-biosynthetic enzymes, including inosine monophosphate dehydrogenase (IMPDH isotype 1 and 2), GMP synthase (GMPS), guanylate kinase (GUK1) and nucleoside diphosphate kinase-A (NDPK-A) at the leading edge in renal cell carcinoma cells. They show significant co-localization at the membrane subdomain, and their co-localization pattern at the membrane is distinct from that of the cell body. While other purine nucleotide biosynthetic enzymes also show significant localization at the leading edge, their co-localization pattern with IMPDH is divergent. In contrast, a key glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), predominantly localized in the cytoplasm. Mechanistically, we found that plasma membrane localization of IMPDH isozymes requires active actin polymerization. Our results demonstrate the formation of a discrete metabolic compartment for localized purine biosynthesis at the leading edge, which may promote localized nucleotide metabolism for cell migration and metastasis in cancers.
Published: 2019
Full Text: View/download PDF

7. Biliary tumorigenic effect on hypopharyngeal cells is significantly enhanced by pH reduction

Author: Jacob I. Tower, Sotirios G. Doukas, Clarence T. Sasaki, Bruno Cardoso, and Dimitra P. Vageli
Subjects: 0301 basic medicine, Cancer Research, medicine.disease_cause, chemistry.chemical_compound, 0302 clinical medicine, STAT3, Cells, Cultured, Original Research, Cancer Biology, biology, Bile acid, Chemistry, laryngopharyngeal reflux, pH, Deoxycholic acid, NF-kappa B, Hypopharyngeal cancer, Hydrogen-Ion Concentration, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Gene Expression Regulation, Neoplastic, Cell Transformation, Neoplastic, Oncology, Proto-Oncogene Proteins c-bcl-2, 030220 oncology & carcinogenesis, Gastroesophageal Reflux, acid, Signal Transduction, STAT3 Transcription Factor, medicine.drug_class, Primary Cell Culture, PH reduction, Models, Biological, lcsh:RC254-282, Bile Acids and Salts, 03 medical and health sciences, medicine, Humans, Radiology, Nuclear Medicine and imaging, Cell Proliferation, bile acids, Messenger RNA, NF‐κB, NF-κB, medicine.disease, Hypopharynx, 030104 developmental biology, Cancer research, biology.protein, Carcinogenesis, hypopharyngeal cancer
Abstract: Biliary reflux has been considered a potential risk factor in upper aerodigestive tract malignancies. It is not yet clearly known how pH affects the bile‐induced activation of NF‐κB and its related oncogenic pathway previously linked to hypopharyngeal carcinogenesis. In this study, repetitive applications of conjugated primary bile acids with unconjugated secondary bile acid, deoxycholic acid (DCA), on human hypopharyngeal primary cells reveal that strongly acidic pH (4.0) optimally enhances the tumorigenic effect of bile, by inducing activation of NF‐κB, STAT3 nuclear translocation, bcl‐2 overexpression and significant overexpression of the oncogenic mRNA phenotype, compared to weakly acidic pH (5.5) or neutral pH (7.0). As the pH becomes less acidic the partially activated primary bile acids and activated DCA begin to exert their influence; however, with significantly less intensity compared to bile acids at strongly acidic pH. Our findings suggest that biliary tumorigenic effect is strongly pH dependent. Controlling pH during reflux events may be therapeutically effective in reducing the potential risk of bile‐induced hypopharyngeal cancer.
Published: 2019

8. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma

Author: Ingrid Grummt, Sergey Mareninov, Holger Bierhoff, Paul S. Mischel, Hiroko Shimada, Lionel M.L. Chow, Nazanin Majd, Makoto Suematsu, Greg Lucey, Alexander Otto Eberhardt, Maya Asano, Yuki Ban, Kaori Kofuji, Eric P. Smith, Eric C. Holland, Noriaki Minami, Ralph J. DeBerardinis, Daisuke Yamashita, Ichiro Nakano, Kazutaka Sumita, Yoshiki Ikeda, Hirofumi Yoshino, Yuki Sugiura, Robert Bachoo, Lisa Lange, Akshiv Malhotra, Mika Sasaki, Risa Kawaguchi, Yonehiro Kanemura, Akiyoshi Hirayama, Annmarie Ramkissoon, William H. Yong, Masaru Mori, Trisha Wise-Draper, Tomoyoshi Soga, Dimitrios Anastasiou, Jumpei Terakawa, Juri Kiyokawa, Tatsuya Ozawa, Hiroaki Wakimoto, Kara Wolfe, Atsuo T. Sasaki, Nobuyuki Onishi, Satoshi Kofuji, Satsuki Ikeda, Craig Horbinski, Takiko Daikoku, Koichi Okumura, Mikako Warren, Shin Morioka, Hideyuki Saya, Naoya Sakamoto, Shuji Kitahara, Oltea Sampetrean, Wataru Yasui, Tomoyuki Mashimo, and Victoria L. Flanary
Subjects: Carcinogenesis, Nucleolus, IMP Dehydrogenase 2, Biology, medicine.disease_cause, Article, 03 medical and health sciences, IMP Dehydrogenase, 0302 clinical medicine, Downregulation and upregulation, Transcription (biology), Cell Line, Tumor, medicine, Humans, education, Cell Proliferation, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, education.field_of_study, Cell growth, Cell Biology, Cell biology, Cell Transformation, Neoplastic, Enzyme, chemistry, RNA, Ribosomal, 030220 oncology & carcinogenesis, Transfer RNA, Glioblastoma, Cell Nucleolus
Abstract: In many cancers, high proliferation rates correlate with elevation of rRNA and tRNA levels, and nucleolar hypertrophy. However, the underlying mechanisms linking increased nucleolar transcription and tumorigenesis are only minimally understood. Here we show that IMP dehydrogenase-2 (IMPDH2), the rate-limiting enzyme for de novo guanine nucleotide biosynthesis, is overexpressed in the highly lethal brain cancer glioblastoma. This leads to increased rRNA and tRNA synthesis, stabilization of the nucleolar GTP-binding protein nucleostemin, and enlarged, malformed nucleoli. Pharmacological or genetic inactivation of IMPDH2 in glioblastoma reverses these effects and inhibits cell proliferation, whereas untransformed glia cells are unaffected by similar IMPDH2 perturbations. Impairment of IMPDH2 activity triggers nucleolar stress and growth arrest of glioblastoma cells even in the absence of functional p53. Our results reveal that upregulation of IMPDH2 is a prerequisite for the occurance of aberrant nucleolar function and increased anabolic processes in glioblastoma, which constitutes a primary event in gliomagenesis.
Published: 2019
Full Text: View/download PDF

9. TGF-β-dependent reprogramming of amino acid metabolism induces epithelial–mesenchymal transition in non-small cell lung cancers

Author: Yasuhiko Nishioka, Seiji Yano, Tomoyoshi Soga, Yasuhiro Yamada, Maki Ohishi, Sho Tabata, Atsuo T. Sasaki, Hisatsugu Goto, Tadaaki Yamada, Fumie Nakasuka, Ayano Ueno, Akiyoshi Hirayama, Ko Umetsu, Takeharu Sakamoto, Masaru Tomita, Hiromichi Ebi, and Masahiro Sugimoto
Subjects: Cancer microenvironment, 0301 basic medicine, Epithelial-Mesenchymal Transition, Lung Neoplasms, QH301-705.5, Procollagen-Proline Dioxygenase, Medicine (miscellaneous), Article, General Biochemistry, Genetics and Molecular Biology, Metastasis, Transcriptome, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Transforming Growth Factor beta, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, medicine, Extracellular, Animals, Humans, Metabolomics, Epithelial–mesenchymal transition, Biology (General), Amino Acids, chemistry.chemical_classification, Chemistry, Gene Expression Profiling, medicine.disease, Cancer metabolism, Cell biology, Amino acid, 030104 developmental biology, 030220 oncology & carcinogenesis, embryonic structures, Female, General Agricultural and Biological Sciences, Non-small-cell lung cancer, Reprogramming, Intracellular
Abstract: Epithelial–mesenchymal transition (EMT)—a fundamental process in embryogenesis and wound healing—promotes tumor metastasis and resistance to chemotherapy. While studies have identified signaling components and transcriptional factors responsible in the TGF-β-dependent EMT, whether and how intracellular metabolism is integrated with EMT remains to be fully elucidated. Here, we showed that TGF-β induces reprogramming of intracellular amino acid metabolism, which is necessary to promote EMT in non-small cell lung cancer cells. Combined metabolome and transcriptome analysis identified prolyl 4-hydroxylase α3 (P4HA3), an enzyme implicated in cancer metabolism, to be upregulated during TGF-β stimulation. Further, knockdown of P4HA3 diminished TGF-β-dependent changes in amino acids, EMT, and tumor metastasis. Conversely, manipulation of extracellular amino acids induced EMT-like responses without TGF-β stimulation. These results suggest a previously unappreciated requirement for the reprogramming of amino acid metabolism via P4HA3 for TGF-β-dependent EMT and implicate a P4HA3 inhibitor as a potential therapeutic agent for cancer., Through metabolome and transcriptome analyses, Nakasuka et al find that TGF-β-induced epithelial–mesenchymal transition (EMT) in non-small cell lung cancer cells is associated with reprogramming of amino acid metabolism. They also identify P4HA3 as a key enzyme involved in these changes altogether providing insights into potential mechanisms of metastasis.
Published: 2021
Full Text: View/download PDF

10. The extrastriate body area is involved in reciprocal imitation of hand gestures, vocalizations, and facial expressions: A univariate and multivariate fMRI study

Author: Yuko Okamoto, Norihiro Sadato, Hiroki C. Tanabe, Ryo Kitada, Akihiro T. Sasaki, Noriaki Yahata, and Takanori Kochiyama
Subjects: Social Psychology, Neural substrate, media_common.quotation_subject, Development, 050105 experimental psychology, Extrastriate body area, 03 medical and health sciences, Behavioral Neuroscience, 0302 clinical medicine, parasitic diseases, Humans, 0501 psychology and cognitive sciences, media_common, Facial expression, Brain Mapping, Gestures, 05 social sciences, Univariate, Imitative Behavior, Magnetic Resonance Imaging, Facial Expression, Action (philosophy), Imitation, Psychology, Neuroscience, 030217 neurology & neurosurgery, Reciprocal, Gesture
Abstract: The extrastriate body area (EBA) in the lateral occipito-temporal cortex has an important role in reciprocal interaction, as it detects congruence between self and other's hand actions. However, it is unclear whether the EBA can detect congruence regardless of the type of action. In the present study, we examined the neural substrate underlying congruence detection of three types of actions: hand gestures, vocalizations, and facial expressions. A univariate analysis revealed a congruency effect, especially for imitating action, for all three types of actions in the EBA. A multi-voxel pattern analysis classifier in the EBA was able to distinguish between initiating interaction from responding to interaction in all experiments. Correspondingly, the congruency effect in the EBA revealed by univariate analysis was stronger for responding to than for initiating interaction. These findings suggest that the EBA might contribute to detect congruence regardless of the body part used (i.e. face or hand) and the type of action (i.e. gestural or vocal). Moreover, initiating and responding to interaction might be processed differently within the EBA. This study highlights the role of the EBA in comparing between self and other's actions beyond hand actions.Running head: Function of EBA in reciprocal imitation.
Published: 2021

11. Mycophenolate Mofetil Hepatotoxicity Associated With Mitochondrial Abnormality in Liver Transplant Recipients and Mice

Author: Daniel W. Thomas, Tania Mitsinikos, Mika Sasaki, Atsuo T. Sasaki, Mikako Warren, and George Yanni
Subjects: Graft Rejection, Male, medicine.medical_specialty, medicine.medical_treatment, Mitochondrial disease, Liver transplantation, Mitochondrion, Gastroenterology, Mice, Internal medicine, Biopsy, medicine, Animals, Humans, Subclinical infection, medicine.diagnostic_test, business.industry, Histology, Mycophenolic Acid, medicine.disease, Liver Transplantation, Mitochondria, Pediatrics, Perinatology and Child Health, Female, Abnormality, Chemical and Drug Induced Liver Injury, business, Liver function tests, Immunosuppressive Agents
Abstract: OBJECTIVES Mycophenolate mofetil (MMF) is a widely used immunosuppressive agent. MMF hepatotoxicity has been reported in non-transplant and renal transplant patients with minimal histologic description. This is the first study describing detailed histology and ultrastructure of MMF hepatotoxicity. METHODS Four liver-transplant recipients (Cases 1-4) were suspected to have MMF hepatotoxicity. Cases 1-3 (two females and one male; 4-17 years) had multiple biopsies for liver function test (LFT) abnormalities. Case 4 (female; 16 years) had a surveillance biopsy. Electron-microscopic examination (EM) was requested on Cases 1-3 for unexplained, persistent LFT elevation and histologic abnormalities despite therapy and Case 4 for unexplained histologic abnormalities despite a stable clinical course. To confirm the pathologic changes in the human allografts, livers from MMF-treated and untreated mice were also reviewed. RESULTS While the allograft biopsies showed nonspecific histologic changes, EM revealed unequivocal mitochondrial abnormalities similar to those seen in primary and secondary mitochondrial disorders. In Cases 1 and 2, LFTs improved after stopping and reducing MMF, respectively. In Case 3, pre- and post-MMF treatment biopsies were performed and only the post-MMF biopsy demonstrated mitochondrial abnormalities. Mitochondrial abnormality in Case 4 was subclinical. The mouse study confirmed that MMF caused various stress changes in the mitochondria; number of mitochondria/cell (mean ± standard deviation; untreated group: 58.25 ± 8.426; MMF-treated group: 76.37 ± 18.66), number of lipid droplets/cell (untreated: 0.9691 ± 1.150; MMF-treated: 3.649 ± 4.143) and sizes of mitochondria (μm, untreated: 0.8550 ± 0.3409; MMF-treated: 0.9598 ± 0.5312) were significantly increased in hepatocytes in the MMF-treated mice compared with the untreated mice (P
Published: 2021

12. Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells

Author: Dimitra P. Vageli, Benjamin L. Judson, Panagiotis G Doukas, and Clarence T. Sasaki
Subjects: 0301 basic medicine, NF-κB, STAT3, 0302 clinical medicine, Pepsin, Epidermal growth factor receptor, Biology (General), Spectroscopy, Cells, Cultured, biology, pH, Chemistry, laryngopharyngeal reflux, NF-kappa B, General Medicine, Hydrogen-Ion Concentration, Computer Science Applications, ErbB Receptors, Cell Transformation, Neoplastic, Proto-Oncogene Proteins c-bcl-2, 030220 oncology & carcinogenesis, hypopharyngeal primary cells, Signal Transduction, STAT3 Transcription Factor, QH301-705.5, Cell Survival, EGFR, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Downregulation and upregulation, Extracellular, Humans, Viability assay, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, PI3K/AKT/mTOR pathway, pepsin, Organic Chemistry, non-acidic reflux, Molecular biology, In vitro, Pepsin A, Hypopharynx, 030104 developmental biology, biology.protein, head and neck cancer, hypopharyngeal cancer
Abstract: Pepsin refluxate is considered a risk factor for laryngopharyngeal carcinogenesis. Non-acidic pepsin was previously linked to an inflammatory and tumorigenic effect on laryngopharyngeal cells in vitro. Yet there is no clear evidence of the pepsin-effect on a specific oncogenic pathway and the importance of pH in this process. We hypothesized that less acidic pepsin triggers the activation of a specific oncogenic factor and related-signalling pathway. To explore the pepsin-effect in vitro, we performed intermittent exposure of 15 min, once per day, for a 5-day period, of human hypopharyngeal primary cells (HCs) to pepsin (1 mg/mL), at a weakly acidic pH of 5.0, a slightly acidic pH of 6.0, and a neutral pH of 7.0. We have documented that the extracellular environment at pH 6.0, and particularly pH 7.0, vs. pH 5.0, promotes the pepsin-effect on HCs, causing increased internalized pepsin and cell viability, a pronounced activation of EGFR accompanied by NF-κB and STAT3 activation, and a significant upregulation of EGFR, AKT1, mTOR, IL1β, TNF-α, RELA(p65), BCL-2, IL6, and STAT3. We herein provide new evidence of the pepsin-effect on oncogenic EGFR activation and its related-signaling pathway at neutral and slightly acidic pH in HCs, opening a window to further explore the prevention and therapeutic approach of laryngopharyngeal reflux disease.
Published: 2021

13. Development of a performance rubric for transanal endoscopic rectal purse-string sutures

Author: K, Teramura, S, Imai, Y, Watanabe, H, Hasegawa, K, Ikeda, Y, Tsukada, Y, Nishizawa, T, Sasaki, S, Mori, Y, Kondo, S, Hirano, and M, Ito
Subjects: Postoperative Complications, Proctectomy, Sutures, Rectal Neoplasms, Rectum, Humans, Laparoscopy, Transanal Endoscopic Surgery
Abstract: Placing a transanal endoscopic rectal purse-string suture (taEPS) is the crucial first component of transanal total mesorectal excision (taTME). However, no structured training is available to improve the procedure-specific skills for taEPS. The aim of this study was to create a performance rubric to improve taEPS skills and provide preliminary evidence for its validity.A performance rubric was created based on technical considerations for taEPS, identified by consulting with taTME surgical and performance assessment experts. Ten independent, blinded raters assessed 10 videotaped taEPS procedures of consecutive taTME cases, at National Cancer Center Hospital East (NCCHE), Chiba, Japan, in January 2018-March 2019 using the rubric and the Global Operative Assessment of Laparoscopic Skills (GOALS). Internal consistency and inter-rater reliabilities were calculated. Videotaped taEPS procedures were timed and assessed by the rubric. Correlation between rubric scores and suturing times were analyzed.The rubric consists of four items: loading the needle (LN), atraumatic needle passage (AP), planned suture path (PS), and overall performance (OA). Videotaped performances were graded on a 3-point Likert scale; scores were calculated as sums of the points. Cronbach's α for internal consistency was 0.713. Inter-rater reliabilities were LN: 0.73, AP: 0.76, PS: 0.71, and OA: 0.70. Rubric and GOALS scores were strongly correlated (r = 0.964, p 0.001). In 112 consecutive taEPS performances, rubric scores were strongly correlated with suturing time (r = - 0.69, p 0.001). Surgeons' experience with taTME was associated with rubric scores and suturing time.This study provides preliminary validation for the taEPS skill performance rubric. The rubric's structured training may facilitate skill acquisition by providing trainees with critical clinical considerations.
Published: 2021

14. Quantitative assessment of HER2 gene amplification of breast cancer using droplet digital PCR

Author: Yasuyuki Seto, Kazutaka Otsuji, T. Sasaki, and Masahiko Tanabe
Subjects: Adult, Pathology, medicine.medical_specialty, Concordance, Breast Neoplasms, In situ hybridization, Biology, Spearman's rank correlation coefficient, Polymerase Chain Reaction, Pathology and Forensic Medicine, law.invention, Breast cancer, law, Gene duplication, medicine, Humans, Digital polymerase chain reaction, Pathology, Molecular, skin and connective tissue diseases, neoplasms, Polymerase chain reaction, In Situ Hybridization, Fluorescence, Aged, Aged, 80 and over, Paraffin Embedding, General Medicine, Oncogene Proteins v-erbB, Genes, erbB-2, Middle Aged, medicine.disease, Molecular biology, Chromosome 17 (human), Female
Abstract: We previously reported the usefulness of droplet digital polymerase chain reaction (ddPCR) for the assessment of Human epithelial growth factor receptor 2 (HER2) gene amplification in breast cancer using formalin-fixed and paraffin-embedded sections. In our previous study, we combined HER2/CEP17 ratio (HER2 gene signals to chromosome 17 signals) with ddPCR and tumor content ratio (TCR) of each sample and determined the HER2 status by adopting a two-dimensional chart. This “ddPCR-TCR method” showed a high concordance with conventional HER2 status. In this study, we updated our method to assess the HER2 status of breast cancer in a more quantitative manner. We combined obtained data of the ddPCR ratio [Rx] and TCR [x]; we calculated “(Rx − 1) / x + 1” for 41 samples with primary breast cancer and named the value led by this formula as “eHER2 (estimated HER2/CEP17 ratio of a tumor cell)”. eHER2 was equivalent to conventional ISH HER2/CEP17 ratio in most cases. eHER2 and ISH ratio showed a very strong correlation (Spearman rank correlation; ρ = 0.70, P < 0.0001). The obtained results indicated that eHER2 is a potential tool for HER2 status diagnosis in breast cancer.
Published: 2021

15. Loss of BRCA1 expression and morphological features associated with BRCA1 promoter methylation status in triple-negative breast cancer

Author: Arisa Morizono, Masahiko Tanabe, Yasuyuki Seto, T. Sasaki, Tetsuo Ushiku, and Masako Ikemura
Subjects: 0301 basic medicine, Mitotic index, endocrine system diseases, Triple Negative Breast Neoplasms, 030105 genetics & heredity, Biology, Epigenesis, Genetic, 03 medical and health sciences, Cytokeratin, Breast cancer, Genetics, medicine, Biomarkers, Tumor, Humans, Epidermal growth factor receptor, skin and connective tissue diseases, Promoter Regions, Genetic, Genetics (clinical), Triple-negative breast cancer, BRCA1 Protein, Promoter, DNA Methylation, medicine.disease, Immunohistochemistry, Androgen receptor, 030104 developmental biology, Cancer research, biology.protein, Female
Abstract: Aberrant DNA methylation in the BRCA1 promoter region causes epigenetic silencing of BRCA1 gene expression, which is critical for breast cancer development. However, how BRCA1 promoter methylation status alters histological features remains poorly understood. Here, we investigated the possibility to predict BRCA1 promoter methylation status based on the morphological and immunohistochemical features of triple-negative breast cancers (TNBCs). The morphological features of 53 TNBCs were evaluated with hematoxylin-eosin staining, with immunohistochemical staining of BRCA1, androgen receptor, p53, cytokeratin 5/6, and epidermal growth factor receptor. BRCA1 promoter methylation status was used to distinguish BRCA1 promoter-methylated tumors (BPMTs) from BRCA1 promoter-unmethylated tumors (BPUTs) dependent on pathological characteristics. BPMTs comprised approximately 26% of the TNBCs. Immunohistochemical analysis found that BRCA1 protein expression was significantly lower in BPMT compared with BPUT (p = 0.016). Morphologically, BPMTs were associated with high mitotic index (p = 0.017), pushing margin (p = 0.017), a circumscribed growth pattern (p = 0.014), and a syncytial growth pattern (p = 0.034) compared with BPUTs. We then assessed the potential of predicting BRCA1 promoter methylation status by using published score systems based on these morphological characteristics. A receiver operating characteristic analysis showed an area under the curve of 0.80. This study found that BRCA1 promoter methylation status could be derived from morphological features and lower BRCA1 expression of TNBCs, which may help identify suitable cases for target treatment with PARP inhibitors.
Published: 2020

16. Ubiquinol-10 Intake Is Effective in Relieving Mild Fatigue in Healthy Individuals

Author: Kei Mizuno, Yasuyoshi Watanabe, Akihiro T. Sasaki, and Kyosuke Watanabe
Subjects: Adult, Male, 0301 basic medicine, Relaxation, Ubiquinol, Elementary cognitive task, Ubiquinone, Trail Making Test, lcsh:TX341-641, Placebo, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Double-Blind Method, motivation, Surveys and Questionnaires, Humans, Medicine, oxidative stress, Nutritional Physiological Phenomena, cognitive function, Coenzyme Q10, Nutrition and Dietetics, Autonomic nerve, Relaxation (psychology), business.industry, ubiquinol, Middle Aged, Healthy Volunteers, 030104 developmental biology, chemistry, Anesthesia, Dietary Supplements, Digit symbol substitution test, autonomic nerve function, Female, fatigue, business, lcsh:Nutrition. Foods and food supply, 030217 neurology & neurosurgery, Food Science
Abstract: Our double-blind, placebo-controlled study evaluated effects of ubiquinol, the reduced form of coenzyme Q10, on mild fatigue in healthy individuals experiencing fatigue in daily life that had continued for more than 1 and less than 6 months. The participants received 100-mg/day (Ubq100, age 44.0 &plusmn, 9.8 years, 14 females and 6 males) or 150-mg/day ubiquinol (Ubq150, age 40.4 &plusmn, 11.8 years, 14 females and 8 males) or placebo (Plc, age 41.3 &plusmn, 13.4 years, 13 females and 7 males) daily for 12 weeks. Measurements of subjective and objective fatigue were conducted by using questionnaires-based fatigue scales/visual analogue scales and autonomic nerve function/biological oxidation index, respectively, prior to the first dosing and every 4 weeks thereafter. Serum ubiquinol level increased three- to four-fold after 4 weeks and remained significantly higher than that after Plc administration throughout the intake period. Although a higher blood level of ubiquinol was observed with Ubq150 than with Ubq100, the difference was not statistically significant. In both Ubq100 and Ubq150 groups, subjective levels of fatigue sensation and sleepiness after cognitive tasks, which consisted of the modified Advanced Trail Making Test, the modified Stroop Color-Word Test, and the Digit Symbol Substitution Test, improved significantly compared with those in the placebo group, suggesting an anti-fatigue effect. The Ubq150 group demonstrated significant improvement compared with the Plc group regarding subjective level of relaxation after task, sleepiness before and after task, motivation for task, and serum level of oxidative stress. Correlation analysis between blood level of ubiquinol and each evaluated effect suggested a positive relationship with relaxation after task, motivation for cognitive task, and parasympathetic activity. The results of the study suggest that ubiquinol intake relieves mild fatigue in healthy individuals.
Published: 2020

17. GTP metabolic reprogramming by IMPDH2: unlocking cancer cells' fuelling mechanism

Author: Atsuo T. Sasaki and Satoshi Kofuji
Subjects: Inosine monophosphate, Anabolism, GTP', Guanosine triphosphate, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, IMP Dehydrogenase, Biosynthesis, Transcription (biology), Neoplasms, JB Reviews, Humans, Molecular Biology, 030304 developmental biology, Cell Proliferation, chemistry.chemical_classification, 0303 health sciences, General Medicine, Cellular Reprogramming, Cell biology, Enzyme, chemistry, 030220 oncology & carcinogenesis, Cancer cell, Guanosine Triphosphate
Abstract: Growing cells increase multiple biosynthetic processes in response to the high metabolic demands needed to sustain proliferation. The even higher metabolic requirements in the setting of cancer provoke proportionately greater biosynthesis. Underappreciated key aspects of this increased metabolic demand are guanine nucleotides and adaptive mechanisms to regulate their concentration. Using the malignant brain tumour, glioblastoma, as a model, we have demonstrated that one of the rate-limiting enzymes for guanosine triphosphate (GTP) synthesis, inosine monophosphate dehydrogenase-2 (IMPDH2), is increased and IMPDH2 expression is necessary for the activation of de novo GTP biosynthesis. Moreover, increased IMPDH2 enhances RNA polymerase I and III transcription directly linking GTP metabolism to both anabolic capacity as well as nucleolar enlargement historically observed as associated with cancer. In this review, we will review in detail the basis of these new discoveries and, more generally, summarize the current knowledge on the role of GTP metabolism in cancer.
Published: 2020

18. The Effect of NNK, A Tobacco Smoke Carcinogen, on the miRNA and Mismatch DNA Repair Expression Profiles in Lung and Head and Neck Squamous Cancer Cells

Author: George Lazopoulos, Sotirios G. Doukas, Aristidis Tsatsakis, Demetrios A. Spandidos, Clarence T. Sasaki, and Dimitra P. Vageli
Subjects: 0301 basic medicine, Lung Neoplasms, Nitrosamines, Cell Survival, NNK, MLH1, DNA Mismatch Repair, Models, Biological, Article, miR-155, 03 medical and health sciences, 0302 clinical medicine, miR-422a, Mismatch DNA repair, Cell Line, Tumor, microRNA, medicine, Humans, RNA, Messenger, Lung cancer, lcsh:QH301-705.5, Carcinogen, business.industry, Squamous Cell Carcinoma of Head and Neck, Gene Expression Profiling, Head and neck cancer, Cancer, General Medicine, medicine.disease, digestive system diseases, Butanones, MSH2, Gene Expression Regulation, Neoplastic, lung cancer, MicroRNAs, 030104 developmental biology, MutS Homolog 2 Protein, lcsh:Biology (General), 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Carcinogens, head and neck cancer, miR-21, business, MutL Protein Homolog 1, tobacco smoke
Abstract: Tobacco smoking is a common risk factor for lung cancer and head and neck cancer. Molecular changes such as deregulation of miRNA expression have been linked to tobacco smoking in both types of cancer. Dysfunction of the Mismatch DNA repair (MMR) mechanism has also been associated with a poor prognosis of these cancers, while a cross-talk between specific miRNAs and MMR genes has been previously proposed. We hypothesized that exposure of lung and head and neck squamous cancer cells (NCI and FaDu, respectively) to tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is capable of altering the expression of MSH2 and MLH1, key MMR components, by promoting specific miRNA deregulation. We found that either a low (1 &mu, M) or high (2 &mu, M) dose of NNK induced significant upregulation of &ldquo, oncomirs&rdquo, miR-21 and miR-155 and downregulation of &ldquo, tumor suppressor&rdquo, miR-422a, as well as the reduction of MMR protein and mRNA expression, in NCI and FaDu, compared to controls. Inhibition of miR-21 restored the NNK-induced reduced MSH2 phenotype in both NCI and FaDu, indicating that miR-21 might contribute to MSH2 regulation. Finally, NNK exposure increased NCI and FaDu survival, promoting cancer cell progression. We provide novel findings that deregulated miR-21, miR-155, and miR-422a and MMR gene expression patterns may be valuable biomarkers for lung and head and neck squamous cell cancer progression in smokers.
Published: 2020

19. Gastrointestinal: Rare malignant biliary stricture with rapid progression

Author: K Hirata, H Naruse, Y Yamamoto, K Hatanaka, K Kinoshita, S Abiko, K Suzuki, K Nakajima, M Katagiri, M Takano, M Ozasa, M Umemura, S Nakajima, K Aoyama, T Sasaki, M Kuwatani, N Sakamoto, S Tanikawa, N Okazaki, and S Tanaka
Subjects: Cholangiopancreatography, Endoscopic Retrograde, Gastrointestinal Tract, Cholestasis, Hepatology, Gastroenterology, Humans, Constriction, Pathologic
Published: 2022
Full Text: View/download PDF

20. Distinct sensitivities of the lateral prefrontal cortex and extrastriate body area to contingency between executed and observed actions

Author: Takanori Kochiyama, Norihiro Sadato, Akihiro T. Sasaki, Yuko Okamoto, Ryo Kitada, and School of Social Sciences
Subjects: Adult, Male, Adolescent, Cognitive Neuroscience, Prefrontal Cortex, Experimental and Cognitive Psychology, Superior parietal lobule, 050105 experimental psychology, Extrastriate body area, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Neuroimaging, Psychology [Social sciences], Cortex (anatomy), medicine, Humans, Middle frontal gyrus, 0501 psychology and cognitive sciences, Social Contingency, Brain Mapping, Sense of agency, 05 social sciences, Imitative Behavior, Magnetic Resonance Imaging, Temporal Lobe, Body Ownership, Neuropsychology and Physiological Psychology, medicine.anatomical_structure, Action (philosophy), Visual Perception, Female, Occipital Lobe, Cues, Psychology, Neuroscience, Photic Stimulation, 030217 neurology & neurosurgery, Gesture
Abstract: Detecting relationships between our own actions and the subsequent actions of others is critical for our social behavior. Self-actions differ from those of others in terms of action kinematics, body identity, and feedback timing. Thus, the detection of social contingency between self-actions and those of others requires comparison and integration of these three dimensions. Neuroimaging studies have highlighted the role of the frontotemporal network in action representation, but the role of each node and their relationships are still controversial. Here, we conducted a functional MRI experiment to test the hypothesis that the lateral prefrontal cortex and lateral occipito-temporal cortex are critical for the integration processes for social contingency. Twenty-four adults performed right finger gestures and then observed them as feedback. We manipulated three parameters of visual feedback: action kinematics (same or different gestures), body identity (self or other), and feedback timing (simultaneous or delayed). Three-way interactions of these factors were observed in the left inferior and middle frontal gyrus (IFG/MFG). These areas were active when self-actions were directly fed back in real-time (i.e., the condition causing a sense of agency), and when participants observed gestures performed by others after a short delay (i.e., the condition causing social contingency). In contrast, the left extrastriate body area (EBA) was sensitive to the concordance of action kinematics regardless of body identity or feedback timing. Body identity × feedback timing interactions were observed in regions including the superior parietal lobule (SPL). An effective connectivity analysis supported the model wherein experimental parameters modulated connections from the occipital cortex to the IFG/MFG via the EBA and SPL. These results suggest that both social contingency and the sense of agency are achieved by hierarchical processing that begins with simple concordance coding in the left EBA, leading to the complex coding of social relevance in the left IFG/MFG. Published version
Published: 2018
Full Text: View/download PDF

21. Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry

Author: Jitendra PS. Sawhney, Veerappa A. Kothiwale, Vikas Bisne, Rajashekhar Durgaprasad, Praveen Jadhav, Manoj Chopda, Velam Vanajakshamma, Ramdhan Meena, Govindan Vijayaraghavan, Kamaldeep Chawla, Jagan Allu, Karen S. Pieper, A. John Camm, Ajay K. Kakkar, Jean-Pierre Bassand, David A. Fitzmaurice, Samuel Z. Goldhaber, Shinya Goto, Sylvia Haas, Werner Hacke, Lorenzo G. Mantovani, Frank Misselwitz, Alexander G.G. Turpie, Martin van Eickels, Freek W.A. Verheugt, Gloria Kayani, Keith A.A. Fox, Bernard J. Gersh, Hector Lucas Luciardi, Harry Gibbs, Marianne Brodmann, Frank Cools, Antonio Carlos Pereira Barretto, Stuart J. Connolly, Alex Spyropoulos, John Eikelboom, Ramon Corbalan, Dayi Hu, Petr Jansky, Jørn Dalsgaard Nielsen, Hany Ragy, Pekka Raatikainen, Jean-Yves Le Heuzey, Harald Darius, Matyas Keltai, Sanjay Kakkar, Jitendra Pal Singh Sawhney, Giancarlo Agnelli, Giuseppe Ambrosio, Yukihiro Koretsune, Carlos Jerjes Sánchez Díaz, Hugo Ten Cate, Dan Atar, Janina Stepinska, Elizaveta Panchenko, Toon Wei Lim, Barry Jacobson, Seil Oh, Xavier Viñolas, Marten Rosenqvist, Jan Steffel, Pantep Angchaisuksiri, Ali Oto, Alex Parkhomenko, Wael Al Mahmeed, David Fitzmaurice, D.Y. Hu, K.N. Chen, Y.S. Zhao, H.Q. Zhang, J.Z. Chen, S.P. Cao, D.W. Wang, Y.J. Yang, W.H. Li, Y.H. Yin, G.Z. Tao, P. Yang, Y.M. Chen, S.H. He, Ying Wang, Yong Wang, G.S. Fu, X. Li, T.G. Wu, X.S. Cheng, X.W. Yan, R.P. Zhao, M.S. Chen, L.G. Xiong, P. Chen, Y. Jiao, Y. Guo, L. Xue, F.Z. Wang, H. Li, Z.M. Yang, C.L. Bai, J. Chen, J.Y. Chen, X. Chen, S. Feng, Q.H. Fu, X.J. Gao, W.N. Guo, R.H. He, X.A. He, X.S. Hu, X.F. Huang, B. Li, J. Li, L. Li, Y.H. Li, T.T. Liu, W.L. Liu, Y.Y. Liu, Z.C. Lu, X.L. Luo, T.Y. Ma, J.Q. Peng, X. Sheng, X.J. Shi, Y.H. Sun, G. Tian, K. Wang, L. Wang, R.N. Wu, Q. Xie, R.Y. Xu, J.S. Yang, L.L. Yang, Q. Yang, Y. Ye, H.Y. Yu, J.H. Yu, T. Yu, H. Zhai, Q. Zhan, G.S. Zhang, Q. Zhang, R. Zhang, Y. Zhang, W.Y. Zheng, B. Zhou, Z.H. Zhou, X.Y. Zhu, S. Kakkar, J.P.S. Sawhney, P. Jadhav, R. Durgaprasad, A.G. Ravi Shankar, R.K. Rajput, K. Bhargava, R. Sarma, A. Srinivas, D. Roy, U.M. Nagamalesh, M. Chopda, R. Kishore, G. Kulkarni, P. Chandwani, R.A. Pothiwala, M. Padinhare Purayil, S. Shah, K. Chawla, V.A. Kothiwale, B. Raghuraman, G. Vijayaraghavan, V.M. Vijan, G. Bantwal, V. Bisne, A. Khan, J.B. Gupta, S. Kumar, D. Jain, S. Abraham, D. Adak, A. Barai, H. Begum, P. Bhattacharjee, M. Dargude, D. Davies, B. Deshpande, P. Dhakrao, V. Dhyani, S. Duhan, M. Earath, A. Ganatra, S. Giradkar, V. Jain, R. Karthikeyan, L. Kasala, S. Kaur, S. Krishnappa, A. Lawande, B. Lokesh, N. Madarkar, R. Meena, P. More, D. Naik, K. Prashanth, M. Rao, N.M. Rao, N. Sadhu, D. Shah, M. Sharma, P. Shiva, S. Singhal, S. Suresh, V. Vanajakshamma, S.G. Panse, Y. Koretsune, S. Kanamori, K. Yamamoto, K. Kumagai, Y. Katsuda, K. Sadamatsu, F. Toyota, Y. Mizuno, I. Misumi, H. Noguchi, S. Ando, T. Suetsugu, M. Minamoto, Hiroshi Oda, K. Shiraishi, S. Adachi, K. Chiba, H. Norita, M. Tsuruta, T. Koyanagi, H. Ando, T. Higashi, K. Okada, S. Azakami, S. Komaki, K. Kumeda, T. Murayama, J. Matsumura, Y. Oba, R. Sonoda, K. Goto, K. Minoda, Y. Haraguchi, H. Suefuji, H. Miyagi, H. Kato, Tadashi Nakamura, Tsugihiro Nakamura, H. Nandate, R. Zaitsu, Yoshihisa Fujiura, A. Yoshimura, H. Numata, J. Ogawa, H. Tatematsu, Y. Kamogawa, K. Murakami, Y. Wakasa, M. Yamasawa, H. Maekawa, S. Abe, H. Kihara, S. Tsunoda, Katsumi Saito, Kazuyuki Saito, T. Fudo, K. Obunai, H. Tachibana, I. Oba, T. Kuwahata, S. Higa, M. Gushiken, T. Eto, H. Yoshida, D. Ikeda, Yoshitake Fujiura, M. Ishizawa, M. Nakatsuka, K. Murata, C. Ogurusu, M. Shimoyama, M. Akutsu, I. Takamura, F. Hoshino, N. Yokota, T. Iwao, K. Tsuchida, M. Takeuchi, Y. Hatori, Y. Kitami, Yoichi Nakamura, R. Oyama, M. Ageta, Hiroyuki Oda, Y. Go, K. Mishima, T. Unoki, S. Morii, Yuhei Shiga, H. Sumi, T. Nagatomo, K. Sanno, K. Fujisawa, Y. Atsuchi, T. Nagoshi, T. Seto, T. Tabuchi, M. Kameko, K. Nii, K. Oshiro, H. Takezawa, S. Nagano, N. Miyamoto, M. Iwaki, Yuichiro Nakamura, M. Fujii, M. Okawa, Masahiko Abe, Masatake Abe, Mitsunori Abe, T. Saito, T. Mito, K. Nagao, J. Minami, T. Mita, I. Sakuma, T. Taguchi, S. Marusaki, H. Doi, M. Tanaka, T. Fujito, M. Matsuta, T. Kusumoto, S. Kakinoki, K. Ashida, N. Yoshizawa, J. Agata, O. Arasaki, M. Manita, M. Ikemura, S. Fukuoka, H. Murakami, S. Matsukawa, Y. Hata, T. Taniguchi, T. Ko, H. Kubo, M. Imamaki, M. Akiyama, M. Inagaki, H. Odakura, T. Ueda, Y. Katsube, A. Nakata, H. Watanabe, M. Techigawara, M. Igarashi, K. Taga, T. Kimura, S. Tomimoto, M. Shibuya, M. Nakano, K. Ito, T. Seo, S. Hiramitsu, H. Hosokawa, M. Hoshiai, M. Hibino, K. Miyagawa, Hajime Horie, N. Sugishita, Yukio Shiga, A. Soma, K. Neya, Tetsuro Yoshida, Tomoki Yoshida, M. Mizuguchi, M. Ishiguro, T. Minagawa, M. Wada, H. Mukawa, F. Okuda, S. Nagasaka, Y. Abe, Sen Adachi, Susumu Adachi, T. Adachi, K. Akahane, T. Amano, K. Aoki, T. Aoyama, H. Arai, S. Arima, T. Arino, H. Asano, T. Asano, J. Azuma, T. Baba, T. Betsuyaku, H. Chibana, H. Date, J. Doiuchi, Y. Emura, M. Endo, Y. Fujii, R. Fujiki, A. Fujisawa, Y. Fujisawa, T. Fukuda, T. Fukui, N. Furukawa, T. Furukawa, W. Furumoto, T. Goto, M. Hamaoka, N. Hanazono, K. Hasegawa, T. Hatsuno, Y. Hayashi, K. Higuchi, K. Hirasawa, H. Hirayama, M. Hirose, S. Hirota, M. Honda, Hideki Horie, T. Ido, O. Iiji, H. Ikeda, K. Ikeda, K. Ikeoka, M. Imaizumi, H. Inaba, T. Inoue, F. Iseki, A. Ishihara, N. Ishioka, N. Ito, T. Iwase, H. Kakuda, J. Kamata, H. Kanai, H. Kanda, M. Kaneko, H. Kano, T. Kasai, T. Kato, Y. Kato, Y. Kawada, K. Kawai, K. Kawakami, S. Kawakami, T. Kawamoto, S. Kawano, J. Kim, T. Kira, H. Kitazawa, H. Kitazumi, T. Kito, T. Kobayashi, T. Koeda, J. Kojima, H. Komatsu, I. Komatsu, Y. Koshibu, T. Kotani, T. Kozuka, Y. Kumai, T. Kumazaki, I. Maeda, K. Maeda, Y. Maruyama, S. Matsui, K. Matsushita, Y. Matsuura, K. Mineoi, H. Mitsuhashi, N. Miura, S. Miyaguchi, S. Miyajima, H. Miyamoto, A. Miyashita, S. Miyata, I. Mizuguchi, A. Mizuno, T. Mori, O. Moriai, K. Morishita, O. Murai, Sho Nagai, Shunichi Nagai, E. Nagata, H. Nagata, A. Nakagomi, S. Nakahara, M. Nakamura, R. Nakamura, N. Nakanishi, T. Nakayama, R. Nakazato, T. Nanke, J. Nariyama, Y. Niijima, H. Niinuma, Y. Nishida, Y. Nishihata, K. Nishino, H. Nishioka, K. Nishizawa, I. Niwa, K. Nomura, S. Nomura, M. Nozoe, T. Ogawa, N. Ohara, M. Okada, K. Okamoto, H. Okita, M. Okuyama, H. Ono, T. Ono, Y. Onuki Pearce, S. Oriso, A. Ota, E. Otaki, Y. Saito, H. Sakai, N. Sakamoto, Y. Sakamoto, Y. Samejima, Y. Sasagawa, H. Sasaguri, A. Sasaki, T. Sasaki, Kazuki Sato, Kiyoharu Sato, M. Sawano, S. Seki, Y. Sekine, Y. Seta, K. Sezaki, N. Shibata, Y. Shiina, H. Shimono, Y. Shimoyama, T. Shindo, H. Shinohara, R. Shinohe, T. Shinozuka, T. Shirai, T. Shiraiwa, Y. Shozawa, T. Suga, C. Sugimoto, Kazuo Suzuki, Keita Suzuki, Shu Suzuki, Shunji Suzuki, Susumu Suzuki, Y. Suzuki, M. Tada, A. Taguchi, T. Takagi, Y. Takagi, K. Takahashi, S. Takahashi, H. Takai, C. Takanaka, S. Take, H. Takeda, K. Takei, K. Takenaka, T. Tana, G. Tanabe, K. Taya, H. Teragawa, S. Tohyo, S. Toru, Y. Tsuchiya, T. Tsuji, K. Tsuzaki, H. Uchiyama, O. Ueda, Y. Ueyama, N. Wakaki, T. Wakiyama, T. Washizuka, M. Watanabe, T. Yamada, T. Yamagishi, H. Yamaguchi, Kenichi Yamamoto, Kentaro Yamamoto, Kunihiko Yamamoto, T. Yamamoto, M. Yamaura, M. Yamazoe, K. Yasui, Y. Yokoyama, K. Yoshida, T.W. Lim, C.K. Ching, C.G. Foo, J.H. Chow, D.D. Chen, F.R. Jaufeerally, Y.M. Lee, G. Lim, W.T. Lim, S. Thng, S.Y. Yap, C. Yeo, S. Oh, H.N. Pak, J.-B. Kim, J.H. Kim, S.-W. Jang, D.H. Kim, D.R. Ryu, S.W. Park, D.-K. Kim, D.J. Choi, Y.S. Oh, M.-C. Cho, S.-H. Kim, H.-K. Jeon, D.-G. Shin, J.S. Park, H.K. Park, S.-J. Han, J.H. Sung, J.-G. Cho, G.-B. Nam, Y.K. On, H.E. Lim, J.J. Kwak, T.-J. Cha, T.J. Hong, S.H. Park, J.H. Yoon, N.-H. Kim, K.-S. Kim, B.C. Jung, G.-S. Hwang, C.-J. Kim, D.B. Kim, J.J. Ahn, H.J. An, H. Bae, A.L. Baek, W.J. Chi, E.A. Choi, E.H. Choi, H.K. Choi, H.S. Choi, S. Han, E.S. Heo, K.O. Her, S.W. Hwang, E.M. Jang, H.-S. Jang, S. Jang, H.-G. Jeon, S.R. Jeon, Y.R. Jeon, H.K. Jeong, I.-A. Jung, Hyeon Jeong Kim, Hyun Ju Kim, Ji Seon Kim, Jung Sook Kim, J.A. Kim, K.T. Kim, M.S. Kim, Sang Hee Kim, Sang Hyun Kim, Y.-I. Kim, C.S. Lee, E.H. Lee, G.H. Lee, H.Y. Lee, H.-Y. Lee, K.H. Lee, K.R. Lee, M.S. Lee, M.-Y. Lee, R.W. Lee, S.E. Lee, S.H. Lee, S. Lee, W.Y. Lee, I.K. Noh, A.R. Park, B.R. Park, H.N. Park, J.H. Park, M. Park, Y. Park, S.-Y. Seo, J. Shim, J.H. Sim, Y.M. Sohn, W.S. Son, Y.S. Son, H.J. Song, H.K. Wi, J.J. Woo, S. Ye, K.H. Yim, K.M. Yoo, E.J. Yoon, S.Y. Yun, P. Angchaisuksiri, S. Chawanadelert, P. Mongkolwongroj, K. Kanokphatcharakun, S. Cheewatanakornkul, T. Laksomya, S. Pattanaprichakul, T. Chantrarat, S. Rungaramsin, S. Silaruks, W. Wongcharoen, K. Siriwattana, K. Likittanasombat, P. Katekangplu, W. Boonyapisit, D. Cholsaringkarl, B. Chatlaong, P. Chattranukulchai, Y. Santanakorn, P. Hutayanon, P. Khunrong, T. Bunyapipat, S. Jai-Aue, P. Kaewsuwanna, P. Bamungpong, S. Gunaparn, S. Hongsuppinyo, R. Inphontan, R. Khattaroek, K. Khunkong, U. Kitmapawanont, C. Kongsin, B. Naratreekoon, S. Ninwaranon, J. Phangyota, A. Phrommintikul, P. Phunpinyosak, K. Pongmorakot, S. Poomiphol, N. Pornnimitthum, S. Pumprueg, S. Ratchasikaew, K. Sanit, K. Sawanyawisuth, B. Silaruks, R. Sirichai, A. Sriwichian, W. Suebjaksing, P. Sukklad, T. Suttana, A. Tangsirira, O. Thangpet, W. Tiyanon, Y. Vorasettakarnkij, T. Wisaratapong, W. Wongtheptien, A. Wutthimanop, S. Yawila, A. Oto, A. Altun, I. Ozdogru, K. Ozdemir, O. Yilmaz, A. Aydinlar, M.B. Yilmaz, E. Yeter, Z. Ongen, M. Cayli, H. Pekdemir, M. Ozdemir, M. Sucu, T. Sayin, M. Demir, H. Yorgun, M. Ersanli, E. Okuyan, D. Aras, H. Abdelrahman, O. Aktas, D. Alpay, F. Aras, M.F. Bireciklioglu, S. Budeyri, M. Buyukpapuc, S. Caliskan, M. Esen, M.A. Felekoglu, D. Genc, B. Ikitimur, E.B. Karaayvaz, S. Kılıç Karataş, S. Okutucu, E. Ozcelik, A. Quisi, H. Sag, L. Sahiner, B.Y. Sayin, T. Seker, D. Uzun Alkan, E. Yildirim, R. Yildirim, F. Yilmaz, V. Yuksekdag, H.L. Luciardi, N. Vensentini, A.C. Ingaramo, G.A. Sambadaro, V. Fernandez Caputi, S.G. Berman, P. Dragotto, A.J. Kleiban, N. Centurion, G. Giacomi, R.A. Ahuad Guerrero, D. Conde, G. Zapata, L.A. Di Paola, J.L. Ramos, R.D. Dran, J. Egido, A.A. Fernandez, M.J. Fosco, S. Sassone, V.A. Sinisi, L.R. Cartasegna, M.A. Berli, O.A. Gomez Vilamajo, F. Ferroni, E.D. Alaguibe, A. Alvarez D'Amelio, C. Arabetti, L. Arias, J.A. Belardi, L. Bergesio, F. Berli, M. Berli, S. Borchowiec, C. Buzzetti, R. Cabrini, V. Campisi, A.L. Cappi, R. Carrizo, F. Colombo Berra, J.P. Costabel, O.J.A. Costamagna, A.A. Damonte, I.N. De Urquiza, F. Diez, M.F. Edén, M. Fanuele, F. Fernandez Voena, M. Foa Torres, C. Funosas, M.P. Giacomi, C.H. Gimenez, E.P. Gurfinkel, M. de L.M. Had, V. Hansen, A.D. Hrabar, M. Ingratta, A. Lopez, G. Maehara, L. Maffei, A. Martinelli, C. Martinelli, J. Matkovich, B. Mautner, A. Meirino, R. Munguia, A. Navarro, V. Novas, G. Perez Prados, J. Pontoriero, R.N. Potito, C. Ricotti, M.A. Rodriguez, F. Rolandi, M.E. Said Palladino, M. Salinger, L.S. Sanziani, P.O. Schygiel, A. Sossich, J.F. Tinto, L. Tonelli, A.L. Tufare, M. Vallejo, M.E. Yunis, M. Zillo, F.J. Zurbrigk, A.C.P. Barretto, D.C. Sobral Filho, J. Jaber, D. Armaganijan, J. Faria Neto, A. Steffens, W. Kunz Sebba Barroso de Souza, J.D. de Souza Neto, J.M. Ribeiro, M. Silveira Teixeira, P.R. Ferreira Rossi, L. Pires, D. Moreira, J.C. Moura Jorge, A. Menezes Lorga Filho, L.C. Bodanese, M. Westerlund Montera, C.H. Del Carlo, T. Da Rocha Rodrigues, F.A. Alves da Costa, A. Lopes, R. Lopes, G.R. Araújo, E.R. Fernandes Manenti, J.F. Kerr Saraiva, J.C. Ferreira Braga, A. Negri, L. Souto, C. Moncada, D. Bertolim Precoma, F. Roquette, G. Reis, R.A. Ramos Filho, E. Lanna Figueiredo, R. Vieira Botelho, C. Munhoz da Fontoura Tavares, C.R. Costantini Frack, J. Abdalla Saad, H.C. Finimundi, C. Pisani, D. Chemello, M. Pereira Martins, C.C. Broilo França, F. Alban, G.B. Aranha Rosito, J.B. de Moura Xavier Moraes Junior, R.T. Tumelero, L. Nigro Maia, R. Simões de Almeida, N.C. do Carmo Borges, L.G. Gomes Ferreira, P. Agliardi, J. Alves de Oliveira Gomes, V. Araujo, M. Arruda Nakazone, T. Barbosa, S. Barroso, E. Belisario Falchetto, H. Bellotti Lopes, M.A. Benez Teixeira Lemos, G. Biazus, L. Borges Queiroz, F.E. Camazzola, M. Caporale, S. Cardoso Boscato, F. Chieza, M.O. Chokr, R. Clemente Mingireanov, N. Codonho Góes, C. Correa, M. Costa, C. Costantini Ortiz, L.S. da Silva, F. da Silva Paulitsch, J.A. da Silveira, E. Daros, G.R. de Araújo, M.I. Del Monaco, C. Dias, M.A. Dias, A.P. Drummond Wainstein, P. Ely Pizzato, D.C. Esteves, P. Fabri, T. Félix Lorenzato Fonseca, E. Fernandes, C. Fonseca, C.R. Frack Costantini, R. Franchin Ferraz, F. Freire, P. Gottardo, D. Guanaes, S. Guizzardi, E. Hettwer Magedanz, F. Igansi, F. Jannuzzi, G. Junior, D. Komar, E.G. Lino, D. Lopes, O. Lourenço da Silva Júnior, E. Lustosa, A.P. Macagnan, M.C. Marinho, M. Mazzoni, G. Melo, L. Mortari, O.M.C.C. Mouco, C. Nanzer Vital, C. Ormundo, S. Oss Emmer, E. Palmegiani, R. Pavani, L. Pereira, V.L. Pereira, R. Perreira, S. Poletti, S.C. Quaia Fortunato, C. Queirantes, N. Ramos Pereira, R.L. Rech, S. Ribeiro, A. Rodrigues, H. Roesch, T. Ruaro Reichert, D. Santos, I. Santos, M. Santos, M.V. Seroqui, S. Silva, L. Soares, L. Spolaor, C. Stoll, N. Toazza Duda, L. Trama, B. Unterkircher, M.V. Valois, T. Vargas, T. Viana, C. Vicente, L. Vidal Armaganijan, R. Vieira Homem, L.G. Vieira Torres, L. Vila Boas, F. Villaça Guimarães Filho, R. Corbalan, G. Eggers, C. Bugueño Gutiérrez, G. Arriagada, S. Potthoff Cardenas, B.A.J. Stockins Fernandez, C. Conejeros, C. Houzvic, P. Marin Cuevas, H. Montecinos, A. Forero, F. Lanas, M. Larico Gómez, G. Charme Vilches, C. Rey, C. Astudillo, J. Aguilar, Y. Campisto, C. Lara, E. Molina, J. Munoz Oyarzon, V. Olguin, M. Vergara, C. Villan, C.J. Sánchez Díaz, J. Illescas Diaz, R. Leal Cantú, M.G. Ramos Zavala, R. Cabrera Jardines, N. Espinola Zavaleta, S. Villarreal Umaña, E. López Rosas, G. Llamas Esperón, G. Pozas, E. Cardona Muñoz, N. Matadamas Hernández, A. Leyva Rendón, N. García Hernández, M. de los Ríos Ibarra, L. Virgen Carrillo, D. López Villezca, C. Hernández Herrera, J.J. López Prieto, R. Gaona Rodríguez, E. Villeda Espinosa, D. Flores Martínez, J. Velasco Barcena, R. Yong, I. Rodríguez Briones, J.L. Leiva Pons, H. Álvarez López, R. Olvera Ruiz, C. Díaz de la Vega, C. Cantú Brito, E. Chuquiure Valenzuela, R. Reyes-Sanchez, A. Bazzoni Ruiz, O. Nandayapa Flores, M. Benavides Gonzalez, R. Arriaga Nava, J.D. Morales Cerda, O. Fierro Fierro, P. Fajardo Campos, T.A.A. Alfaro, S. Altamirano Bellorin, R. Avena, M. Chavarria, I. Espinosa, F. Flores Silva, R.H. Garcia Nava, K. Godoy, E.J. Gonzalez Felix, C.L. Gonzalez Garcia, L.G. Gonzalez Salas, P. Guajardo, S. Hernandez Gonzalez, T. Izquierdo, M.C. Mancilla Ortiz, D. Martinez Vasquez, N. Mendoza, J. Morales, N. Nikitina, S. Ochoa Aybar, A. Ortiz, P. Padilla Macias, F. Perez, J.A. Perez Sanchez, S. Piña Toledano, M. Ramos Gonzalez, C. Rivera Ramos, V. Roa Castro, G. Romero Cardona, M. Ruiz Cornejo, A. Salinas, G. Santana, P. Sida Perez, A.C. Tovar Castaneda, R. Trujillo Cortes, M. Brodmann, K. Lenz, H. Drexel, J. Foechterle, C. Hagn, A. Podczeck-Schweighofer, K. Huber, M. Winkler, B. Schneeweiß, A. Gegenhuber, W. Lang, S. Eichinger-Hasenauer, P. Kaserer, J. Sykora, H. Rasch, M. Pichler, E. Schaflinger, B. Strohmer, R. Breier, K.-M. Ebner, L. Eischer, F. Freihoff, A. Lischka-Lindner, T. Mark, A. Mirtl, A. Said, C. Stöcklöcker, B. Vogel, A. Vonbank, C. Wöhrer, D. Zanolin, F. Cools, G. Paparella, P. Vandergoten, J.-L. Parqué, L. Capiau, G. Vervoort, B. Wollaert, P. Desfontaines, G. Mairesse, T. Boussy, P. Godart, A. De Wolf, J. Voet, A. Heyse, G. Hollanders, W. Anné, J. Vercammen, P. Purnode, I. Blankoff, D. Faes, Y. Balthazar, M. Beutels, P. Maréchal, S. Verstraete, O. Xhaet, H. Striekwold, J. Thoeng, K. Hermans, B. Alzand, A.-K. Ascoop, F. Banaeian, A.-M. Barbuto, A.C. Billiaux, M. Blockmans, C. Bouvy, C. Brike, H. Capiau, T. Casier, A. Conde Y Bolado, D. De Cleen, M. De Coninck, M. de Vos, N. de Weerdt, M. Delforge, M. Delvigne, D. Denie, K. Derycker, E. Deweerd, F. Dormal, S. Drieghe, M. Everaert, T. Eykerman, E. Feys, M. Ghekiere, F. Gits, S. Hellemans, L. Helvasto, C. Jacobs, S. Lips, I. Mestdagh, J. Nimmegeers, V. Piamonte, P. Pollet, A. Postolache, M. Raepers, E. Raymenants, J. Richa, H. Rombouts, J. Salembier, C. Scheurwegs, O. Semeraro, N. Simons, C. Smessaert, W. Smolders, I. Stockman, S. Tahon, V. Thyssen, G. Tincani, F. Van Durme, D. Van Lier, H. Vandekerckhove, Y. Vandekerckhove, D. Vandenbroeck, A. Vandorpe, E. Vanhalst, B. Vanhauwaert, C. Vantomme, L. Vergauwen, H. Verloove, T. Vydt, T. Weyn, P. Jansky, P. Reichert, R. Spacek, V. Machova, E. Zidkova, O. Ludka, J. Olsr, L. Kotik, K. Plocova, B. Racz, R. Ferkl, J. Hubac, I. Kotik, Z. Monhart, H. Burianova, O. Jerabek, J. Pisova, I. Petrova, V. Dedek, M. Honkova, P. Podrazil, J. Spinar, J. Vitovec, M. Novak, J. Lastuvka, V. Durdil, P. Antonova, L. Bockova, J. Bultas, J. Chlumsky, L. Dastychova, T. Drasnar, J. Honek, M. Horejsi, V. Hubacova, L. Janska, I. Kopeckova, R. Kratochvilova, E. Krcova, R. Labrova, A. Lindourkova, J. Lipoldova, H. Lubanda, A. Ludkova, L. Mahdalikova, M. Majerníkova, D. Michalik, P. Potuznik, E. Prochazkova, A. Sulc, J. Sveceny, M. Valtova, M. Zidek, J. Zika, J. Nielsen, H. Nielsen, S. Husted, U. Hintze, S. Rasmussen, A. Bremmelgaard, J. Markenvard, J. Boerger, J. Solgaard, P. Simonsen, T. Loekkegaard, M. Bruun, J. Mertz, H. Domínguez, K. Skagen, K. Egstrup, H. Ibsen, I. Raymond, T. Bang-Hansen, C. Ellervik, E. Eriksen, L. Jensen, M. Jensen, M. Leth, A. Nygaard, J. Park, M. Schou, A. Therkelsen, J. Tilma, K. Vesterager, P. Raatikainen, J. Airaksinen, O. Arola, J. Koistinen, H. Nappila, K. Peltomäki, V. Rasanen, T. Vasankari, J.-Y. Le Heuzey, M. Galinier, Y. Gottwalles, F. Paganelli, P. Loiselet, J.-J. Muller, M.B. Koujan, A. Marquand, S. Destrac, O. Piot, N. Delarche, J.-P. Cebron, S. Boveda, M. Guenoun, D. Guedj-Meynier, D. Galley, J. Ohayon, S. Assouline, M. Zuber, P. Amarenco, E. Ellie, J. Kadouch, P.-Y. Fournier, J.-P. Huberman, M. Lemaire, G. Rodier, L. Milandre, X. Vandamme, I. Sibon, J.-P. Neau, M.H. Mahagne, A. Mielot, M. Bonnefoy, J.-B. Churet, V. Navarre, F. Sellem, G. Monniot, J.-P. Boyes, B. Doucet, M. Martelet, D. Obadia, B. Crousillat, J. Mouallem, E. Bearez, P. Nazeyrollas, J.P. Brugnaux, A. Fedorowsky, F. Casassus, J.-B. Berneau, F. Chemin, N. Falvo, J.-M. Perron, J.-E. Poulard, A. Barreau, C. Beltra, E. Corrihons, N. Decarsin, B. Dubois, E. Ducasse, X. Giry, A. Kemmel, S. Ledure, N. Lemaire, F. Robin, N. Rosolin, D. Sanchez, A. Suissa, H. Darius, G. Königer, J. Purr, U. Gerbaulet, B.-T. Kellner, A. Kopf, T. Schäfer, H. Zauzig, P. Riegel, H. Hohensee, E. Eißfeller, W. Eder, G. Rehling, D. Glatzel, S. Zutz, G.-U. Heinz, H. Menke, A. Pustelnik, P. Sandow, N. Ludwig, H. Wiswedel, W. Wildenauer, C. Axthelm, T. Schwarz, A. Babyesiza, G. Stuchlik, H.-H. Zimny, M. Kropp, F. Kahl, A. Caspar, S. Omankowsky, T. Läßig, H.-J. Hartmann, G. Lehmann, H.-W. Bindig, G. Hergdt, D. Reimer, J. Hauk, W. Dorsch, J. Dshabrailov, H. Michel, K.-A. Rapp, R. Vormann, P. Mayer, U. Horstmeier, V. Eissing, H. Hey, H. Leuchtgens, V. Lilienweiß, K. Kolitsch, C. Schubert, H. Lauer, T. Buchner, G. Brauer, S. Kamin, K. Müller, M. Abdel-Qader, S. Baumbach, H.-H. Ebert, C. Schwencke, S. Schellong, P. Bernhardt, L. Karolyi, B. Sievers, W. Haverkamp, P. Salbach, J.-U. Röhnisch, S. Schoen, W. Erdle, T. Mueller, H. Mueller, V. Mitrovic, Z. Babjakova, K. Bergner, S. Boehme, K. Bonin, D. Buckert, F. Busch, U. Dichristin, S. Diez, A. Fleck, K. Flint, H. Floegel, C. Fritz, R. Frommhold, J. Gehre, J. Geyer, A. Grytzmann, M. Hahn, K. Helgert, K. Hubert, K. Kirchner-Volker, V. Klein, D. Kroll, A. Krueger, R. Lehmann, L. Mann, A. Maselli, G. Menken, K. Mikes, H. Mortan, N. Nasser, D. Nicolaus, A. Plauskat, L. Pomper, A. Quietzsch, C. Ravenhorst, C. Reichelt, C. Reimer, B. Schaefer, S. Scharrer, K. Schirmer, K. Schmidt, R. Schoene, J. Schulze, M. Schuppe, S. Simon, S. Sommer, K. Spranger, A. Talkenberger, K. Tauber, A. Tetlak, T. Toennishoff, R. Voelkel-Babyesiza, B. Voigts, U. Weiser, S. Wesendorf, S. Wildenauer, T. Wolf, J. Wurziger, J. Zak, H.-D. Zauzig, S. Ziefle, S. Zincke, M. Keltai, S. Vangel, G. Szalai, B. Merkely, S. Kancz, Z. Boda, A. Nagy, Z. Laszlo, A. Matoltsy, B. Gaszner, P. Polgar, T. Habon, E. Noori, G. Juhasz, N. Kanakaridisz, I. Szentpeteri, F. Juhasz, A. Vertes, A. Papp, Z. May, J. Ferenczi, M. Egyutt, E. Kis, G. Engelthaler, G. Szantai, E. Fulop, P. Gombos, D. Gulyas, P. Jen, E. Kiralyhazine Gyorke, M. Kovacs, S. Kovacsne Levang, S. Marianna, Z. Radics, N. Sydó, R. Szalo, A. Szilagyi, F. Sztanyik, B. Vandrus, G. Agnelli, G. Ambrosio, E. Tiraferri, R. Santoro, S. Testa, G. Di Minno, M. Moia, T.M. Caimi, G. Martini, M. Tessitori, R. Cappelli, D. Poli, R. Quintavalla, F. Melone, F. Cosmi, A. Pizzini, G. Piseddu, R. Fanelli, C. Latella, R. Santi, L. Pancaldi, R. De Cristofaro, G. Palareti, A. De Blasio, J. Salerno Uriarte, F. Minetti, E.M. Pogliani, L.M. Lonati, M. Accogli, N. Ciampani, S. Malengo, M. Feola, A. Raisaro, L. Fattore, P. Grilli, F. Germini, M. Settimi, M. Alunni, G. Duranti, L. Tedeschi, G. Baglioni, G. Avanzino, M. Berardi, V. Pannacci, A. Giombolini, S. Nicoli, T. Scarponi, B. Allasia, P. Ricciarini, R. Nasorri, A. Argena, P. Bossolasco, P. Ronchini, A. Filippi, F. Tradati, C. Bulla, L. Donzelli, L. Foppa, M.L. Bottarelli, A. Tomasello, A. Mauric, C. Femiano, R. Reggio, F. Lillo, A. Mariani, F. Forcignanò, M. Volpe, M. D'Avino, M.G. Bongiorni, S. Severi, A. Capucci, C. Lodigiani, E. Salomone, G. Serviddio, C. Tondo, P. Golino, C. Mazzone, S. Iacopino, V. Pengo, M. Galvani, L. Moretti, P. Ambrosino, E. Banfi, V. Biagioli, A. Bianchi, G. Boggian, M. Breschi, S. Brusorio, F. Calcagnoli, G. Campagna, M. Carpenedo, C. Ciabatta, G. Ciliberti, G. Cimmino, C. D'Arienzo, L. Di Gennaro, M. Fedele, P.M. Ferrini, K. Granzow, G. Guazzaloca, F. Guerra, A. Lo Buglio, S. Longo, F. Macellari, E. Mesolella, E. Mollica Poeta, P. Occhilupo, V. Oriana, G. Rangel, L. Salomone, A. Scaccianoce, C. Scarone, L. Segreti, G. Sottilota, R. Villani, C. Zecca, H. ten Cate, J.H. Ruiter, H. Klomps, M. Bongaerts, M.G.C. Pieterse, C. Guldener, J.-P. Herrman, G. Lochorn, A. Lucassen, H. Adriaansen, S.H.K. The, P.R. Nierop, P.A.M. Hoogslag, W. Hermans, B.E. Groenemeijer, W. Terpstra, C. Buiks, L.V.A. Boersma, M. Boersma-Slootweg, F. Bosman, M. Bosschaert, S. Bruin, I. Danse, J. De Graaf, J. de Graauw, M. Debordes, S. Dols, F. Geerlings, K. Gorrebeeck, A. Jerzewski, W. Jetten, M. Kelderman, T. Kloosterman, E.M. Koomen, J. Krikken, P. Melman, R. Mulder, A. Pronk, A. Stallinga-de Vos, J. te Kaat, P. Tonino, B. Uppelschoten, R. van de Loo, T. van der Kley, G. van Leeuwen, J.J. van Putten, L. Westerman, D. Atar, E. Berge, P.A. Sirnes, E. Gjertsen, T. Hole, K. Erga, A. Hallaråker, G. Skjelvan, A. Østrem, B. Ghezai, A. Svilaas, P. Christersson, T. Øien, S. Høegh Henrichsen, J. Berg-Johansen, J.E. Otterstad, H. Antonsen, K. Ausen, H. Claussen, I. Dominguez, A. Jekthammer, A.B. Lensebraaten, V. Nilsen, M. O'Donovan, K. Ringdalen, S. Strand, J. Stepinska, R. Korzeniak, A. Gieroba, M. Biedrzycka, Marcin Ogorek, B. Wozakowska-Kaplon, K. Loboz-Grudzien, J. Kosior, W. Supinski, J. Kuzniar, R. Zaluska, J. Hiczkiewicz, L. Swiatkowska-Byczynska, L. Kucharski, M. Gruchala, P. Minc, M. Olszewski, G. Kania, M. Krzciuk, Z. Lajkowski, B. Ostrowska-Pomian, J. Lewczuk, E. Zinka, A. Karczmarczyk, M. Chmielnicka-Pruszczynska, M. Trusz-Gluza, G. Opolski, M. Bronisz, Michal Ogorek, G. Glanowska, P. Ruszkowski, K. Jaworska, R. Sciborski, B. Okopien, P. Kukla, I. Wozniak-Skowerska, K. Galbas, K. Cymerman, J. Jurowiecki, P. Miekus, W. Myszka, S. Mazur, R. Lysek, J. Baszak, T. Rusicka-Piekarz, G. Raczak, E. Domanska, J. Nessler, J. Lesnik, M. Ambicka, D. Andrzejewski, J. Araminowicz, A. Barszcz, R. Bartkowiak, J. Bartnik, M. Basiak, E. Bekieszczuk, L. Bernat, L. Biedrzycki, A. Biernacka, D. Blaszczyk, E. Broton, W. Brzozowski, M. Brzustowska, R. Bzymek, A. Chmielowski, P. Chojnowski, R. Cichomski, K. Cieslak, A. Cieszynska, B. Curyllo, M. Czamara, L. Danilowicz-Szymanowicz, B. Dolecka, L. Drelich, B. Dudzik-Richter, T. Dybala, M. Dziuba, W. Faron, M. Figura-Chmielewska, A. Frankiewicz, W. Gadzinski, E. Gasior, B. Gosciniecka, P. Gutknecht, M. Guziewicz, A. Jackun-Podlesna, G. Jaguszewska, J. Jankielewicz, A. Jaremczuk-Kaczmarczyk, M. Jargiello-Baszak, A. Jarzebowski, E. Jaskulska-Niedziela, M. Jaworska-Drozdowska, J. Kabat, A. Kaczmarzyk-Radka, K. Kalin, R. Kaliszczak, M. Kiliszek, M. Klata, M. Kluczewski, I. Kobielusz-Gembala, E. Kochanska, D. Kociolek, A. Kolodzinska, A. Komlo, A. Konopka, E. Korczowska, E. Kowal, H.K. Kowalczyk, E. Kremis, D. Kruczyk, A. Krzesiak-Lodyga, M. Krzyzanowski, W. Kurdzielewicz, D. Kustrzycka-Kratochwil, D. Lesniewska-Krynska, J. Leszczynski, E. Lewicka, E. Lichota, K. Lip, M. Loboz-Rudnicka, J. Luka, A. Lysek-Jozefowicz, M. Machnikowska, K. Majewska, R. Mariankowski, A. Markiewicz, M. Mazur, A. Metzgier-Gumiela, E. Miedlar, M. Mielcarek, J. Neubauer-Geryk, J. Niedek, A. Niemirycz-Makurat, A. Nowak, S. Nowak, B. Opielowska-Nowak, M. Ozgowicz, A. Pawelska-Buczen, E. Pawlik-Rak, R. Piotrowicz, P. Ptaszynski, A. Raczynska, W. Rogowski, J. Romanek, R. Romaszkiewicz, P. Rostoff, N. Roszczyk, D. Rozewska-Furmanek, J. Rychta, B. Rzyczkowska, A. Sidor, J. Skalska, M. Smichura, M. Splawski, P. Staneta, E. Staniszewska, J. Starak-Marciniak, M. Stopyra-Poczatek, M. Sukiennik-Kujawa, J. Szafranski, P. Szalecki, A. Szczepanska, W. Szkrobka, E. Szuchnik, A. Szulowska, G. Szumczyk-Muszytowska, M. Szwoch, T. Traczyk, M. Troszczynska, G. Trzcinski, S. Tybura, P. Walasik, M. Wegrzynowska, K. Wesolowska, W. Wieczorek, A. Wierzbicka, P. Wilczewski, M. Wilgat-Szecowka, P. Wojewoda, L. Wojnowski, M. Wrobel, K. Zakutynska-Kowalczyk, M. Zyczynska-Szmon, E. Panchenko, V. Eltishcheva, R. Libis, S. Tereshchenko, S. Popov, G. Kamalov, D. Belenky, A. Zateyshchikova, E. Kropacheva, A. Kolesnikova, K. Nikolaev, L. Egorova, A. Khokhlov, E. Yakupov, M. Poltavskaya, D. Zateyshchikov, O. Drapkina, A. Vishnevsky, O. Barbarash, O. Miller, E. Aleksandrova, P. Chizhov, M. Sergeev, E. Shutemova, E. Mazur, K. Zrazhevskiy, T. Novikova, V. Kostenko, Y. Moiseeva, E. Polkanova, K. Sobolev, M. Rossovskaya, G. Zubeeva, Y. Shapovalova, O. Nagibovich, A. Edin, A. Agakhanyan, R. Batalov, Y. Belenkova, F. Bitakova, S. Chugunnaya, A. Dumikyan, S. Erofeeva, E. Gorbunova, T. Gorshkova, A. Gubanov, M. Gurmach, Y. Ivanova, T. Kolesova, D. Konyushenko, O. Korneeva, O. Kropova, P. Kuchuk, O. Kungurtseva, T. Kupriyanova, B. Kurylo, M. Kuvanova, O. Lebedeva, E. Lileeva, O. Machilskaya, T. Medvedeva, G. Monako, I. Motylev, G. Nagibovich, E. Novikova, Y. Orlov, Y. Osmolovskaya, A. Ovsannikova, D. Platonov, S. Rachkova, O. Sinitsina, S. Speshilova, O. Suslova, A. Ushakov, O. Volodicheva, O. Zemlianskaia, I. Zhirov, E. Zhuravleva, I. Zotova, X. Viñolas, P. Alvarez Garcia, M.F. López Fernández, L. Tercedor, S. Tranche Iparraguirre, P. Torán Monserrat, E. Márquez Contreras, J. Isart Rafecas, J. Motero Carrasco, P. García Pavía, C. Gómez Pajuelo, C. Moro Serrano, L.F. Iglesias Alonso, A. Grande Ruiz, J. Mercé Klein, J.R. Gonzalez Juanatey, G. Barón Esquivias, I. Monte Collado, H. Palacín Piquero, C. Brotons Cuixart, M. Rodríguez Morató, J. Bayo I Llibre, C. Corros Vicente, M. Vida Gutierrez, F. Epelde Gonzalo, C.A. Almeida Fernández, N. Del Val Plana, E. Escrivá Montserrat, J.J. Montero Alía, M. Barreda González, M.A. Moleiro Oliva, J. Iglesias Sanmartín, M. Jiménez González, M. Rodriguez Álvarez, J. Herreros Melenchon, T. Ripoll Vera, F. Ridocci Soriano, L. Garcia Riesco, M.D. Marco Macian, J. Quiles Granado, M. Jimenez Navarro, J. Cosin Sales, J.V. Vaquer Perez, M. Vazquez Caamano, M.F. Arcocha Torres, G. Marcos Gomez, A. Iñiguez Romo, M.A. Prieto Diaz, Carmela Alonso, Concepcion Alonso, D. Alvarez, M. Alvarez, M. Amaro, N. Andere, J. Aracil Villar, R. Armitano Ochoa, A. Austria, S. Barbeira, E. Barraquer Feu, A. Bartes, V. Becerra Munoz, F.J. Bermudez Jimenez, A. Branjovich Tijuan, J. Cabeza Ramirez, M. Cabrera Ramos, E. Calvo Martinez, M. Campo Moreno, G. Cancho Corchado, M. Casanova Gil, M. Castillo Orive, D. Castro Fernandez, M. Cebollada del Misterio, R. Codinachs Alsina, A. Cortada Cabrera, J. Costa Pinto Prego de Faria, S. Costas, M.I. Cotilla Marco, M. Dachs, C.M. Diaz Lopez, A. Domenech Borras, A. Elorriaga Madariaga, A. Espallargas, M. Fernandez, E. Fernandez Escobar, E. Fernandez Mas, A. Ferrer, J. Fosch, M. Garcia Bermudez, V. Garcia Millan, M. Gavira Saenz, C. Gines Garcia, C. Gomez, Y. Gomez Perez, A. Gonzales Segovia, P. Gonzalez, L. Grigorian, A. Guerrero Molina, M. del C. Gutierrez del Val, B. Herrero Maeso, E. Hevia Rodriguez, A. Iglesias Garcia, M.J. Jimenez Fernandez, B. Jimeno Besa, P. Juan Salvadores, M.B. Lage Bouzamayor, I. Lasuncion, L.E. Lezcano Gort, M. Llobet Molina, M. Lopez, A. Manzanal Rey, J. Mara Guerra, S. Marcus, A. Martin Vila, M. Martinez Mena, P. Mazon, F. Mendez Zurita, G. Millán, M. Molina, P. Montero Alia, D. Montes, M. Moure Gonzalez, R.B. Munoz Munoz, A. Negrete Palma, H.N. Orellana Figueroa, V.M. Ortega, C. Ortiz Cortes, D. Otero Tomera, N. Palomo Merchan, I. Pareja Ibar, E. Pena Garcia, M. Pereda Armayor, M. Perez Carasa, I. Prieto, V. Quintern, R. Renom, L.M. Rincon Diaz, V. Rios, L. Riquelme Sola, R. Rivera, X. Robiro Robiro, M. Roca, C. Roca Saumell, C. Rodrigo, E. Rodriguez, M. Rodriguez Garcia, S. Saez Jimenez, P. Sanchez Calderon, L. Sanchez Mendez, S. Sanchez Parra, C. Santolaya, M.R. Senan Sanz, A. Seoane Blanco, E. Serralvo, N. Sierra, C. Simon Valero, J. Sorribes Lopez, M. Teixido Fontanillas, M. Terns Riera, G. Tobajas, C. Torres, J. Torres Marques, M. Ubeda Pastor, M. Rosenqvist, A. Wirdby, J. Linden, K. Henriksson, M. Elmersson, A. Egilsson, U. Börjesson, G. Svärd, B. Liu, A. Lindh, L.-B. Olsson, M. Gustavsson, Lars Andersson, Lisbeth Andersson, L. Benson, C. Bothin, A. Hajimirsadeghi, K. Kadir, M. Ericsson, A. Ohlsson, H. Lindvall, P. Svensson, K. Thorne, H. Handel, P. Platonov, B. Eriksson, I. Timberg, K. Romberg, M. Crisby, J.-E. Karlsson, S.A. Jensen, A. Andersson, L. Malmqvist, B. Martinsson, F. Bernsten, J. Engdahl, J. Thulin, A. Hot-Bjelac, P. Stalby, H. Aaröe, E. Ahbeck, H. Ahlmark, F. Al-Khalili, G. Bonkowski, S. Dzeletovic, A.-B. Ekstrand, G.-B. Eriksson, K. Floren, C. Grässjö, S. Hahn, P. Jaensson, B. Jansson, J.-H. Jansson, R.-M. Kangert, A. Koch, D. Kusiak, A. Lettenström, A. Lindberg, C.-J. Lindholm, A. Mannermyr, K. Mansson, M. Millborg, C. Nilsson, A.-M. Ohlin, A. Olofsson, A. Osberg, A. Pedersen, K. Risbecker, K. Rosenberg, J. Samuelsson, M. Shayesteh, K. Skoglund, M. Stjernberg, C. Thorsen, J. Steffel, J.H. Beer, J. Debrunner, D. Amstutz, J. Bruegger, G. Elise, A. Grau, A. Guinand, I. Henriette, E. Saga, S. Winnik, A. Parkhomenko, I. Rudyk, V. Tseluyko, O. Karpenko, S. Zhurba, I. Kraiz, I. Kupnovytska, N. Serediuk, Y. Mostovoy, O. Ushakov, O. Koval, I. Kovalskyi, Y. Svyshchenko, O. Sychov, M. Stanislavchuk, O. Kraydashenko, A. Yagensky, S. Tykhonova, I. Fushtey, R. Belegai, G. Berko, L. Burdeuna, O. Chabanna, I. Daniuk, A. Ivanov, E. Kamenska, P. Kaplan, O. Khyzhnyak, S. Kizim, O. Matova, O. Medentseva, V. Mochonyi, M. Mospan, V. Nemtsova, T. Ovdiienko, O. Palamarchuk, M. Pavelko, R. Petrovskyy, D. Plevak, O. Proshak, S. Pyvovar, L. Rasputina, O. Romanenko, O. Romanova, A. Sapatyi, O. Shumakov, R. Stets, L. Todoriuk, V. Varenov, D. Fitzmaurice, N. Chauhan, D. Goodwin, P. Saunders, R. Evans, J. Leese, P.S. Jhittay, A. Ross, M.S. Kainth, G. Pickavance, J. McDonnell, A. Williams, T. Gooding, H. Wagner, S. Suryani, A. Singal, S. Sircar, R. Bilas, P. Hutchinson, A. Wakeman, M. Stokes, N. Paul, M. Aziz, C. Ramesh, P. Wilson, S. Franklin, S. Fairhead, J. Thompson, V. St Joseph, G. Taylor, D. Tragen, D. Seamark, C. Paul, M. Richardson, A. Jefferies, H. Sharp, H. Jones, C. Giles, M. Page, O. Oginni, J. Aldegather, S. Wetherwell, W. Lumb, P. Evans, F. Scouller, N. Macey, Y. Stipp, R. West, S. Thurston, P. Wadeson, J. Matthews, P. Pandya, A. Gallagher, T. Railton, B. Sinha, D. Russell, J.A. Davies, P. Ainsworth, C.P. Jones, P. Weeks, J. Eden, D. Kernick, W. Murdoch, L. Lumley, R.P. Patel, S.W. Wong, M. Saigol, K. Ladha, K. Douglas, D.F. Cumberlidge, C. Bradshaw, G. Van Zon, K.P. Jones, M.J. Thomas, E. Watson, B. Sarai, N. Ahmad, W. Willcock, J. Cairns, S. Sathananthan, N. de Kare-Silver, A. Gilliland, E. Strieder, A. Howitt, B. Vishwanathan, N. Bird, D. Gray, M. Clark, J. Bisatt, J. Litchfield, E. Fisher, T. Fooks, A.R. Kelsall, E. Alborough, J. Wakeling, M. Parfitt, K. Milne, S. Rogers, R. Priyadharshan, J.L. Oliver, E. Davies, S. Abushal, M. Jacobs, C. Hutton, N.I. Walls, R. Thompson, C. Chigbo, S.M.A. Zaidi, M. Howard, K.C. Butter, S. Barrow, H. Little, I.U. Haq, L. Gibbons, S. Glencross, A.J. McLeod, K. Poland, C. Mulholland, A. Warke, P. Conn, G. Burns, R.N. Smith, S. Lowe, R. Kamath, H.S. Dau, J. Webster, I. Hodgins, S. Vercoe, P.C. Roome, H. Pinnock, J.R.A. Patel, A. Ali, N. Hart, R. Davies, E. Stuart, C.A. Neden, M. Danielsen, R. Heath, P. Sharma, S. Galloway, C. Hawkins, R. Oliver, M. Aylward, S. Mannion, M. Braddick, D. Edwards, A.C. Rothwell, A. Sabir, F. Choudhary, S. Khalaque, A. Wilson, S. Peters, W. Coulson, N. Roberts, A. Heer, S. Coates, B. Ward, D. Jackson, S. Walton, D. Shepherd, M. Sterry, T. Wong, M. Boon, R. Bunney, R. Haria-Shah, R.T. Baron, S. Davies, T. Schatzberger, N. Hargreaves, T. Stephenson, H. Choi, R. Batson, L. Lucraft, T. Myhill, S. Estifano, D. Geatch, J. Wilkinson, R. Veale, K. Forshaw, T. Davies, K. Zaman, P. Vinson, C. Liley, M. Bandrapalli, P. McGinty, R. Wastling, P. McEleny, A. Beattie, P. Cooke, M. Wong, J. Gunasegaram, M. Pugsley, S. Ahmad, C. A'Court, J. Ayers, J. Bennett, S. Cartwright, S. Dobson, C. Dooldeniya, A. Flynn, R. Fox, J. Goram, A. Halpin, A. Hay, P. Jacobs, L. Jeffers, L. Lomax, I. Munro, R. Muvva, M. Nadaph, K. Powell, S. Randfield, D. Redpath, R. Reed, M. Rickenbach, G. Rogers, P.B. Saunders, C. Seamark, J. Shewring, P. Simmons, H. Simper, H. Stoddart, A. Sword, N. Thomas, A. Thomson, H. Gibbs, A. Blenkhorn, B. Singh, W. Van Gaal, W. Abhayaratna, R. Lehman, P. Roberts-Thomson, J. Kilian, D. Coulshed, A. Catanchin, D. Colquhoun, H. Kiat, D. Eccleston, J. French, L. Zimmett, B. Ayres, T. Phan, P. Blombery, D. Crimmins, D. O'Donnell, A. Choi, P. Astridge, M. Arstall, N. Jepson, M. Binnekamp, A. Lee, J. Rogers, G. Starmer, P. Carroll, J. Faunt, A. Aggarwala, L. Barry, C. Batta, R. Beveridge, A. Black, M. Bonner, J. Boys, E. Buckley, M. Campo, L. Carlton, A. Connelly, B. Conway, D. Cresp, H. Dimitri, S. Dixon, M. Dolman, M. Duroux, M. Eskandari, R. Eslick, A. Ferreira-Jardim, T. Fetahovic, D. Fitzpatrick, R. Geraghty, J. Gibbs, T. Grabek, M.H. Modi, K. Hayes, M.P. Hegde, L. Hesketh, B. Hoffmann, B. Jacobson, K. Johnson, C. Juergens, I. Kassam, V. Lawlor, M. Lehman, S. Lehman, D. Leung, S. Mackay, M. MacKenzie, C. McCarthy, C. McIntosh, L. McKeon, H. Morrison, C. Mussap, J.-D. Myers, V. Nagalingam, G. Oldfield, V. O'May, J. Palmer, L. Parsons, K. Patching, T. Patching, V. Paul, M. Plotz, S. Preston, H. Rashad, M. Ratcliffe, S. Raynes, J. Rose, L. Sanders, M. Seremetkoska, H. Setio, S. Shone, P. Shrestha, C. Singh, C. Singleton, N. Stoyanov, S. Sutcliffe, K. Swaraj, J. Tarrant, S. Thompson, I.M. Tsay, M. Vorster, A. Waldman, L. Wallis, E. Wilford, K. Wong, S.J. Connolly, A. Spyropoulos, J. Eikelboom, R. Luton, M. Gupta, A.S. Pandey, S. Cheung, R. Leader, P. Beaudry, F. Ayala-Paredes, J. Berlingieri, J. Heath, G. Poirier, M. Du Preez, R. Nadeau, G. Dresser, R. Dhillon, T. Hruczkowski, B. Schweitzer, B. Coutu, P. Angaran, P. MacDonald, S. Vizel, S. Fikry, R. Parkash, A. Lavoie, J. Cha, B. Ramjattan, J. Bonet, K. Ahmad, L. Aro, T. Aves, K. Beaudry, C. Bergeron, J. Bigcanoe, N. Bignell, L. Breakwell, E. Burke, L. Carroll, B. Clarke, T. Cleveland, S. Daheb, P. Dehghani, I. Denis, Z. Djaidani, P. Dorian, S. Douglass, J. Dunnigan, A. Ewert, D. Farquhar, A. Fearon, L. Ferleyko, D. Fournier, B. Fox, M.-C. Grenier, W. Gulliver, K. Haveman, C. Hines, K. Hines, A.M. Jackson, C. Jean, G. Jethoo, R. Kahlon, S. Kelly, R. Kim, V. Korley, J. Kornder, L. Kwan, J. Largy, C. Lewis, S. Lewis, I. Mangat, R. Moor, J. Navratil, I. Neas, J. Otis, R. Otis, M. Pandey, F. Petrie, A. Pinter, M. Raines, P. Roberts, M. Robinson, G. Sas, S. Schulman, L. Snell, S. Spearson, J. Stevenson, T. Trahey, S. Wong, D. Wright, H. Ragy, A. Abd El-Aziz, S.K. Abou Seif, M.G. El Din, S. El Etriby, A. Elbahry, A. El-Etreby, M. Elkhadem, A. Katta, T. Khairy, A. Mowafy, M. Nawar, A. Ohanissian, A. Reda, M. Reda, H. Salem, N. Sami, S. Samir, M. Setiha, M. Sobhy, A. Soliman, N. Taha, M. Tawfik, E. Zaatout, D. Kettles, J. Bayat, H. Siebert, A. Horak, Y. Kelfkens, R. Garda, T. Pillay, M. Guerra, L. van Zyl, H. Theron, A. Murray, R. Louw, D. Greyling, P. Mntla, V. Ueckermann, R. Loghdey, S. Ismail, F. Ahmed, J. Engelbrecht, A. Ramdass, S. Maharajh, W. Oosthuysen, G. Angel, C. Bester, M. Booysen, C. Boshoff, C. Cannon, S. Cassimjee, C. Chami, G. Conway, A. Davids, L. de Meyer, G. Du Plessis, T. Ellis, L. Henley, M. Karsten, E. Loyd, J. Marks, L. Mavhusa, M. Mostert, A. Page, L. Rikhotso, M. Salie, J. Sasto, F. Shaik, A. Skein, L. Smith, G. Tarr, T. Tau, F. van Zyl, W. Al Mahmeed, G. Yousef, A. Agrawal, M. Nathani, M. Ibrahim, E.M. Esheiba, R. Singh, A. Naguib, M. Abu-Mahfouz, M. Al Omairi, A. Al Naeemi, R. Maruthanayagam, N. Bazargani, A. Wassef, R. Gupta, M. Khan, B. Subbaraman, A. Abdul, A. Al Mulla, S. El Bardisy, P. Haridas, S. Jadhav, K. Magdaluyo, M. Makdad, I. Maqsood, R. Mohamed, N. Sharma, R. Sharma, M. Thanzeel, S.Z. Goldhaber, R. Canosa, P. Rama, E. Blumberg, J. Garcia, P. Mullen, V. Wilson, A. Quick, K. Ferrick, W.M. Kutayli, M. Cox, M. Franco, S. Falkowski, R. Mendelson, M. Williams, S. Miller, S. Beach, A. Alfieri, T. Gutowski, I. Haque, R. Reddy, W. Ahmed, P. Delafontaine, D. Diercks, D. Theodoro, K. Remmel, M. Alberts, R. Ison, H. Noveck, P. Duffy, S. Pitta, D. Nishijima, C. Treasure, N. Asafu-Adjaye, K. Ball, M. Bartlett, M. Bentley, S. Bowers, A. Brown, A. Browne, J. Cameron-Watts, M. Canova, D. Cassidy, K. Cervellione, S. Congal, J. DePauw, A. Dickerson, M. Eley, L. Evans, S. Felpel, K. Ferdinand, D. Fielder, P. Gentry, A. Haideri, F. Hakimi, T. Harbour, E. Hartranft, B. Hawkins, M. Headlee, L. Henson, C. Herrick, T. Hicks, S. Jasinski, A. Jones, L. Jones, P. Jones, S. Karl, M. Keeling, J. Kerr, P. Knowles, J. Langdon, M. Lay, J.A. Lee, T. Lincoln, E. Malone, A. Merliss, D. Merritt, J. Minardo, B. Mooso, C. Orosco, V. Palumbo, M. Parker, T. Parrott, S. Paserchia, G. Pearl, J. Peterson, N. Pickelsimer, T. Purcell, J. Raynor, S. Raziano, C. Richard, T. Richardson, C. Robertson, A. Sage, T. Sanghera, P. Shaw, J. Shoemaker, K. Smith, B. Stephanie, A. Thatcher, H. Theobald, N. Thompson, L. Treasure, T. Tripti, C. Verdi, V. Worthy, Cardiology, ACS - Heart failure & arrhythmias, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service de cardiologie, Sawhney, J, Kothiwale, V, Bisne, V, Durgaprasad, R, Jadhav, P, Chopda, M, Vanajakshamma, V, Meena, R, Vijayaraghavan, G, Chawla, K, Allu, J, Pieper, K, John Camm, A, Kakkar, A, Bassand, J, Fitzmaurice, D, Goldhaber, S, Goto, S, Haas, S, Hacke, W, Mantovani, L, Misselwitz, F, Turpie, A, van Eickels, M, Verheugt, F, Kayani, G, Fox, K, Gersh, B, Luciardi, H, Gibbs, H, Brodmann, M, Cools, F, Barretto, A, Connolly, S, Spyropoulos, A, Eikelboom, J, Corbalan, R, Hu, D, Jansky, P, Nielsen, J, Ragy, H, Raatikainen, P, Le Heuzey, J, Darius, H, Keltai, M, Kakkar, S, Agnelli, G, Ambrosio, G, Koretsune, Y, Sanchez Diaz, C, Ten Cate, H, Atar, D, Stepinska, J, Panchenko, E, Lim, T, Jacobson, B, Oh, S, Vinolas, X, Rosenqvist, M, Steffel, J, Angchaisuksiri, P, Oto, A, Parkhomenko, A, Al Mahmeed, W, Chen, K, Zhao, Y, Zhang, H, Chen, J, Cao, S, Wang, D, Yang, Y, Li, W, Yin, Y, Tao, G, Yang, P, Chen, Y, He, S, Wang, Y, Fu, G, Li, X, Wu, T, Cheng, X, Yan, X, Zhao, R, Chen, M, Xiong, L, Chen, P, Jiao, Y, Guo, Y, Xue, L, Wang, F, Li, H, Yang, Z, Bai, C, Chen, X, Feng, S, Fu, Q, Gao, X, Guo, W, He, R, He, X, Hu, X, Huang, X, Li, B, Li, J, Li, L, Li, Y, Liu, T, Liu, W, Liu, Y, Lu, Z, Luo, X, Ma, T, Peng, J, Sheng, X, Shi, X, Sun, Y, Tian, G, Wang, K, Wang, L, Wu, R, Xie, Q, Xu, R, Yang, J, Yang, L, Yang, Q, Ye, Y, Yu, H, Yu, J, Yu, T, Zhai, H, Zhan, Q, Zhang, G, Zhang, Q, Zhang, R, Zhang, Y, Zheng, W, Zhou, B, Zhou, Z, Zhu, X, Ravi Shankar, A, Rajput, R, Bhargava, K, Sarma, R, Srinivas, A, Roy, D, Nagamalesh, U, Kishore, R, Kulkarni, G, Chandwani, P, Pothiwala, R, Padinhare Purayil, M, Shah, S, Raghuraman, B, Vijan, V, Bantwal, G, Khan, A, Gupta, J, Kumar, S, Jain, D, Abraham, S, Adak, D, Barai, A, Begum, H, Bhattacharjee, P, Dargude, M, Davies, D, Deshpande, B, Dhakrao, P, Dhyani, V, Duhan, S, Earath, M, Ganatra, A, Giradkar, S, Jain, V, Karthikeyan, R, Kasala, L, Kaur, S, Krishnappa, S, Lawande, A, Lokesh, B, Madarkar, N, More, P, Naik, D, Prashanth, K, Rao, M, Rao, N, Sadhu, N, Shah, D, Sharma, M, Shiva, P, Singhal, S, Suresh, S, Panse, S, Kanamori, S, Yamamoto, K, Kumagai, K, Katsuda, Y, Sadamatsu, K, Toyota, F, Mizuno, Y, Misumi, I, Noguchi, H, Ando, S, Suetsugu, T, Minamoto, M, Oda, H, Shiraishi, K, Adachi, S, Chiba, K, Norita, H, Tsuruta, M, Koyanagi, T, Ando, H, Higashi, T, Okada, K, Azakami, S, Komaki, S, Kumeda, K, Murayama, T, Matsumura, J, Oba, Y, Sonoda, R, Goto, K, Minoda, K, Haraguchi, Y, Suefuji, H, Miyagi, H, Kato, H, Nakamura, T, Nandate, H, Zaitsu, R, Fujiura, Y, Yoshimura, A, Numata, H, Ogawa, J, Tatematsu, H, Kamogawa, Y, Murakami, K, Wakasa, Y, Yamasawa, M, Maekawa, H, Abe, S, Kihara, H, Tsunoda, S, Saito, K, Fudo, T, Obunai, K, Tachibana, H, Oba, I, Kuwahata, T, Higa, S, Gushiken, M, Eto, T, Yoshida, H, Ikeda, D, Ishizawa, M, Nakatsuka, M, Murata, K, Ogurusu, C, Shimoyama, M, Akutsu, M, Takamura, I, Hoshino, F, Yokota, N, Iwao, T, Tsuchida, K, Takeuchi, M, Hatori, Y, Kitami, Y, Nakamura, Y, Oyama, R, Ageta, M, Go, Y, Mishima, K, Unoki, T, Morii, S, Shiga, Y, Sumi, H, Nagatomo, T, Sanno, K, Fujisawa, K, Atsuchi, Y, Nagoshi, T, Seto, T, Tabuchi, T, Kameko, M, Nii, K, Oshiro, K, Takezawa, H, Nagano, S, Miyamoto, N, Iwaki, M, Fujii, M, Okawa, M, Abe, M, Saito, T, Mito, T, Nagao, K, Minami, J, Mita, T, Sakuma, I, Taguchi, T, Marusaki, S, Doi, H, Tanaka, M, Fujito, T, Matsuta, M, Kusumoto, T, Kakinoki, S, Ashida, K, Yoshizawa, N, Agata, J, Arasaki, O, Manita, M, Ikemura, M, Fukuoka, S, Murakami, H, Matsukawa, S, Hata, Y, Taniguchi, T, Ko, T, Kubo, H, Imamaki, M, Akiyama, M, Inagaki, M, Odakura, H, Ueda, T, Katsube, Y, Nakata, A, Watanabe, H, Techigawara, M, Igarashi, M, Taga, K, Kimura, T, Tomimoto, S, Shibuya, M, Nakano, M, Ito, K, Seo, T, Hiramitsu, S, Hosokawa, H, Hoshiai, M, Hibino, M, Miyagawa, K, Horie, H, Sugishita, N, Soma, A, Neya, K, Yoshida, T, Mizuguchi, M, Ishiguro, M, Minagawa, T, Wada, M, Mukawa, H, Okuda, F, Nagasaka, S, Abe, Y, Adachi, T, Akahane, K, Amano, T, Aoki, K, Aoyama, T, Arai, H, Arima, S, Arino, T, Asano, H, Asano, T, Azuma, J, Baba, T, Betsuyaku, T, Chibana, H, Date, H, Doiuchi, J, Emura, Y, Endo, M, Fujii, Y, Fujiki, R, Fujisawa, A, Fujisawa, Y, Fukuda, T, Fukui, T, Furukawa, N, Furukawa, T, Furumoto, W, Goto, T, Hamaoka, M, Hanazono, N, Hasegawa, K, Hatsuno, T, Hayashi, Y, Higuchi, K, Hirasawa, K, Hirayama, H, Hirose, M, Hirota, S, Honda, M, Ido, T, Iiji, O, Ikeda, H, Ikeda, K, Ikeoka, K, Imaizumi, M, Inaba, H, Inoue, T, Iseki, F, Ishihara, A, Ishioka, N, Ito, N, Iwase, T, Kakuda, H, Kamata, J, Kanai, H, Kanda, H, Kaneko, M, Kano, H, Kasai, T, Kato, T, Kato, Y, Kawada, Y, Kawai, K, Kawakami, K, Kawakami, S, Kawamoto, T, Kawano, S, Kim, J, Kira, T, Kitazawa, H, Kitazumi, H, Kito, T, Kobayashi, T, Koeda, T, Kojima, J, Komatsu, H, Komatsu, I, Koshibu, Y, Kotani, T, Kozuka, T, Kumai, Y, Kumazaki, T, Maeda, I, Maeda, K, Maruyama, Y, Matsui, S, Matsushita, K, Matsuura, Y, Mineoi, K, Mitsuhashi, H, Miura, N, Miyaguchi, S, Miyajima, S, Miyamoto, H, Miyashita, A, Miyata, S, Mizuguchi, I, Mizuno, A, Mori, T, Moriai, O, Morishita, K, Murai, O, Nagai, S, Nagata, E, Nagata, H, Nakagomi, A, Nakahara, S, Nakamura, M, Nakamura, R, Nakanishi, N, Nakayama, T, Nakazato, R, Nanke, T, Nariyama, J, Niijima, Y, Niinuma, H, Nishida, Y, Nishihata, Y, Nishino, K, Nishioka, H, Nishizawa, K, Niwa, I, Nomura, K, Nomura, S, Nozoe, M, Ogawa, T, Ohara, N, Okada, M, Okamoto, K, Okita, H, Okuyama, M, Ono, H, Ono, T, Onuki Pearce, Y, Oriso, S, Ota, A, Otaki, E, Saito, Y, Sakai, H, Sakamoto, N, Sakamoto, Y, Samejima, Y, Sasagawa, Y, Sasaguri, H, Sasaki, A, Sasaki, T, Sato, K, Sawano, M, Seki, S, Sekine, Y, Seta, Y, Sezaki, K, Shibata, N, Shiina, Y, Shimono, H, Shimoyama, Y, Shindo, T, Shinohara, H, Shinohe, R, Shinozuka, T, Shirai, T, Shiraiwa, T, Shozawa, Y, Suga, T, Sugimoto, C, Suzuki, K, Suzuki, S, Suzuki, Y, Tada, M, Taguchi, A, Takagi, T, Takagi, Y, Takahashi, K, Takahashi, S, Takai, H, Takanaka, C, Take, S, Takeda, H, Takei, K, Takenaka, K, Tana, T, Tanabe, G, Taya, K, Teragawa, H, Tohyo, S, Toru, S, Tsuchiya, Y, Tsuji, T, Tsuzaki, K, Uchiyama, H, Ueda, O, Ueyama, Y, Wakaki, N, Wakiyama, T, Washizuka, T, Watanabe, M, Yamada, T, Yamagishi, T, Yamaguchi, H, Yamamoto, T, Yamaura, M, Yamazoe, M, Yasui, K, Yokoyama, Y, Yoshida, K, Ching, C, Foo, C, Chow, J, Chen, D, Jaufeerally, F, Lee, Y, Lim, G, Lim, W, Thng, S, Yap, S, Yeo, C, Pak, H, Jang, S, Kim, D, Ryu, D, Park, S, Choi, D, Oh, Y, Cho, M, Kim, S, Jeon, H, Shin, D, Park, J, Park, H, Han, S, Sung, J, Cho, J, Nam, G, On, Y, Lim, H, Kwak, J, Cha, T, Hong, T, Yoon, J, Kim, N, Kim, K, Jung, B, Hwang, G, Kim, C, Ahn, J, An, H, Bae, H, Baek, A, Chi, W, Choi, E, Choi, H, Heo, E, Her, K, Hwang, S, Jang, E, Jang, H, Jeon, S, Jeon, Y, Jeong, H, Jung, I, Kim, H, Kim, M, Kim, Y, Lee, C, Lee, E, Lee, G, Lee, H, Lee, K, Lee, M, Lee, R, Lee, S, Lee, W, Noh, I, Park, A, Park, B, Park, M, Park, Y, Seo, S, Shim, J, Sim, J, Sohn, Y, Son, W, Son, Y, Song, H, Wi, H, Woo, J, Ye, S, Yim, K, Yoo, K, Yoon, E, Yun, S, Chawanadelert, S, Mongkolwongroj, P, Kanokphatcharakun, K, Cheewatanakornkul, S, Laksomya, T, Pattanaprichakul, S, Chantrarat, T, Rungaramsin, S, Silaruks, S, Wongcharoen, W, Siriwattana, K, Likittanasombat, K, Katekangplu, P, Boonyapisit, W, Cholsaringkarl, D, Chatlaong, B, Chattranukulchai, P, Santanakorn, Y, Hutayanon, P, Khunrong, P, Bunyapipat, T, Jai-Aue, S, Kaewsuwanna, P, Bamungpong, P, Gunaparn, S, Hongsuppinyo, S, Inphontan, R, Khattaroek, R, Khunkong, K, Kitmapawanont, U, Kongsin, C, Naratreekoon, B, Ninwaranon, S, Phangyota, J, Phrommintikul, A, Phunpinyosak, P, Pongmorakot, K, Poomiphol, S, Pornnimitthum, N, Pumprueg, S, Ratchasikaew, S, Sanit, K, Sawanyawisuth, K, Silaruks, B, Sirichai, R, Sriwichian, A, Suebjaksing, W, Sukklad, P, Suttana, T, Tangsirira, A, Thangpet, O, Tiyanon, W, Vorasettakarnkij, Y, Wisaratapong, T, Wongtheptien, W, Wutthimanop, A, Yawila, S, Altun, A, Ozdogru, I, Ozdemir, K, Yilmaz, O, Aydinlar, A, Yilmaz, M, Yeter, E, Ongen, Z, Cayli, M, Pekdemir, H, Ozdemir, M, Sucu, M, Sayin, T, Demir, M, Yorgun, H, Ersanli, M, Okuyan, E, Aras, D, Abdelrahman, H, Aktas, O, Alpay, D, Aras, F, Bireciklioglu, M, Budeyri, S, Buyukpapuc, M, Caliskan, S, Esen, M, Felekoglu, M, Genc, D, Ikitimur, B, Karaayvaz, E, Kilic Karatas, S, Okutucu, S, Ozcelik, E, Quisi, A, Sag, H, Sahiner, L, Sayin, B, Seker, T, Uzun Alkan, D, Yildirim, E, Yildirim, R, Yilmaz, F, Yuksekdag, V, Vensentini, N, Ingaramo, A, Sambadaro, G, Fernandez Caputi, V, Berman, S, Dragotto, P, Kleiban, A, Centurion, N, Giacomi, G, Ahuad Guerrero, R, Conde, D, Zapata, G, Di Paola, L, Ramos, J, Dran, R, Egido, J, Fernandez, A, Fosco, M, Sassone, S, Sinisi, V, Cartasegna, L, Berli, M, Gomez Vilamajo, O, Ferroni, F, Alaguibe, E, Alvarez D'Amelio, A, Arabetti, C, Arias, L, Belardi, J, Bergesio, L, Berli, F, Borchowiec, S, Buzzetti, C, Cabrini, R, Campisi, V, Cappi, A, Carrizo, R, Colombo Berra, F, Costabel, J, Costamagna, O, Damonte, A, De Urquiza, I, Diez, F, Eden, M, Fanuele, M, Fernandez Voena, F, Foa Torres, M, Funosas, C, Giacomi, M, Gimenez, C, Gurfinkel, E, De, L, Hansen, V, Hrabar, A, Ingratta, M, Lopez, A, Maehara, G, Maffei, L, Martinelli, A, Martinelli, C, Matkovich, J, Mautner, B, Meirino, A, Munguia, R, Navarro, A, Novas, V, Perez Prados, G, Pontoriero, J, Potito, R, Ricotti, C, Rodriguez, M, Rolandi, F, Said Palladino, M, Salinger, M, Sanziani, L, Schygiel, P, Sossich, A, Tinto, J, Tonelli, L, Tufare, A, Vallejo, M, Yunis, M, Zillo, M, Zurbrigk, F, Sobral Filho, D, Jaber, J, Armaganijan, D, Faria Neto, J, Steffens, A, Kunz Sebba Barroso de Souza, W, de Souza Neto, J, Ribeiro, J, Silveira Teixeira, M, Ferreira Rossi, P, Pires, L, Moreira, D, Moura Jorge, J, Menezes Lorga Filho, A, Bodanese, L, Westerlund Montera, M, Del Carlo, C, Da Rocha Rodrigues, T, Alves da Costa, F, Lopes, A, Lopes, R, Araujo, G, Fernandes Manenti, E, Kerr Saraiva, J, Ferreira Braga, J, Negri, A, Souto, L, Moncada, C, Bertolim Precoma, D, Roquette, F, Reis, G, Ramos Filho, R, Lanna Figueiredo, E, Vieira Botelho, R, Munhoz da Fontoura Tavares, C, Costantini Frack, C, Abdalla Saad, J, Finimundi, H, Pisani, C, Chemello, D, Pereira Martins, M, Broilo Franca, C, Alban, F, Aranha Rosito, G, de Moura Xavier Moraes Junior, J, Tumelero, R, Nigro Maia, L, Simoes de Almeida, R, do Carmo Borges, N, Gomes Ferreira, L, Agliardi, P, Alves de Oliveira Gomes, J, Araujo, V, Arruda Nakazone, M, Barbosa, T, Barroso, S, Belisario Falchetto, E, Bellotti Lopes, H, Benez Teixeira Lemos, M, Biazus, G, Borges Queiroz, L, Camazzola, F, Caporale, M, Cardoso Boscato, S, Chieza, F, Chokr, M, Clemente Mingireanov, R, Codonho Goes, N, Correa, C, Costa, M, Costantini Ortiz, C, da Silva, L, da Silva Paulitsch, F, da Silveira, J, Daros, E, de Araujo, G, Del Monaco, M, Dias, C, Dias, M, Drummond Wainstein, A, Ely Pizzato, P, Esteves, D, Fabri, P, Felix Lorenzato Fonseca, T, Fernandes, E, Fonseca, C, Frack Costantini, C, Franchin Ferraz, R, Freire, F, Gottardo, P, Guanaes, D, Guizzardi, S, Hettwer Magedanz, E, Igansi, F, Jannuzzi, F, Junior, G, Komar, D, Lino, E, Lopes, D, Lourenco da Silva Junior, O, Lustosa, E, Macagnan, A, Marinho, M, Mazzoni, M, Melo, G, Mortari, L, Mouco, O, Nanzer Vital, C, Ormundo, C, Oss Emmer, S, Palmegiani, E, Pavani, R, Pereira, L, Pereira, V, Perreira, R, Poletti, S, Quaia Fortunato, S, Queirantes, C, Ramos Pereira, N, Rech, R, Ribeiro, S, Rodrigues, A, Roesch, H, Ruaro Reichert, T, Santos, D, Santos, I, Santos, M, Seroqui, M, Silva, S, Soares, L, Spolaor, L, Stoll, C, Toazza Duda, N, Trama, L, Unterkircher, B, Valois, M, Vargas, T, Viana, T, Vicente, C, Vidal Armaganijan, L, Vieira Homem, R, Vieira Torres, L, Vila Boas, L, Villaca Guimaraes Filho, F, Eggers, G, Bugueno Gutierrez, C, Arriagada, G, Potthoff Cardenas, S, Stockins Fernandez, B, Conejeros, C, Houzvic, C, Marin Cuevas, P, Montecinos, H, Forero, A, Lanas, F, Larico Gomez, M, Charme Vilches, G, Rey, C, Astudillo, C, Aguilar, J, Campisto, Y, Lara, C, Molina, E, Munoz Oyarzon, J, Olguin, V, Vergara, M, Villan, C, Illescas Diaz, J, Leal Cantu, R, Ramos Zavala, M, Cabrera Jardines, R, Espinola Zavaleta, N, Villarreal Umana, S, Lopez Rosas, E, Llamas Esperon, G, Pozas, G, Cardona Munoz, E, Matadamas Hernandez, N, Leyva Rendon, A, Garcia Hernandez, N, de los Rios Ibarra, M, Virgen Carrillo, L, Lopez Villezca, D, Hernandez Herrera, C, Lopez Prieto, J, Gaona Rodriguez, R, Villeda Espinosa, E, Flores Martinez, D, Velasco Barcena, J, Yong, R, Rodriguez Briones, I, Leiva Pons, J, Alvarez Lopez, H, Olvera Ruiz, R, Diaz de la Vega, C, Cantu Brito, C, Chuquiure Valenzuela, E, Reyes-Sanchez, R, Bazzoni Ruiz, A, Nandayapa Flores, O, Benavides Gonzalez, M, Arriaga Nava, R, Morales Cerda, J, Fierro Fierro, O, Fajardo Campos, P, Alfaro, T, Altamirano Bellorin, S, Avena, R, Chavarria, M, Espinosa, I, Flores Silva, F, Garcia Nava, R, Godoy, K, Gonzalez Felix, E, Gonzalez Garcia, C, Gonzalez Salas, L, Guajardo, P, Hernandez Gonzalez, S, Izquierdo, T, Mancilla Ortiz, M, Martinez Vasquez, D, Mendoza, N, Morales, J, Nikitina, N, Ochoa Aybar, S, Ortiz, A, Padilla Macias, P, Perez, F, Perez Sanchez, J, Pina Toledano, S, Ramos Gonzalez, M, Rivera Ramos, C, Roa Castro, V, Romero Cardona, G, Ruiz Cornejo, M, Salinas, A, Santana, G, Sida Perez, P, Tovar Castaneda, A, Trujillo Cortes, R, Lenz, K, Drexel, H, Foechterle, J, Hagn, C, Podczeck-Schweighofer, A, Huber, K, Winkler, M, Schneeweiss, B, Gegenhuber, A, Lang, W, Eichinger-Hasenauer, S, Kaserer, P, Sykora, J, Rasch, H, Pichler, M, Schaflinger, E, Strohmer, B, Breier, R, Ebner, K, Eischer, L, Freihoff, F, Lischka-Lindner, A, Mark, T, Mirtl, A, Said, A, Stocklocker, C, Vogel, B, Vonbank, A, Wohrer, C, Zanolin, D, Paparella, G, Vandergoten, P, Parque, J, Capiau, L, Vervoort, G, Wollaert, B, Desfontaines, P, Mairesse, G, Boussy, T, Godart, P, De Wolf, A, Voet, J, Heyse, A, Hollanders, G, Anne, W, Vercammen, J, Purnode, P, Blankoff, I, Faes, D, Balthazar, Y, Beutels, M, Marechal, P, Verstraete, S, Xhaet, O, Striekwold, H, Thoeng, J, Hermans, K, Alzand, B, Ascoop, A, Banaeian, F, Barbuto, A, Billiaux, A, Blockmans, M, Bouvy, C, Brike, C, Capiau, H, Casier, T, Conde Y Bolado, A, De Cleen, D, De Coninck, M, de Vos, M, de Weerdt, N, Delforge, M, Delvigne, M, Denie, D, Derycker, K, Deweerd, E, Dormal, F, Drieghe, S, Everaert, M, Eykerman, T, Feys, E, Ghekiere, M, Gits, F, Hellemans, S, Helvasto, L, Jacobs, C, Lips, S, Mestdagh, I, Nimmegeers, J, Piamonte, V, Pollet, P, Postolache, A, Raepers, M, Raymenants, E, Richa, J, Rombouts, H, Salembier, J, Scheurwegs, C, Semeraro, O, Simons, N, Smessaert, C, Smolders, W, Stockman, I, Tahon, S, Thyssen, V, Tincani, G, Van Durme, F, Van Lier, D, Vandekerckhove, H, Vandekerckhove, Y, Vandenbroeck, D, Vandorpe, A, Vanhalst, E, Vanhauwaert, B, Vantomme, C, Vergauwen, L, Verloove, H, Vydt, T, Weyn, T, Reichert, P, Spacek, R, Machova, V, Zidkova, E, Ludka, O, Olsr, J, Kotik, L, Plocova, K, Racz, B, Ferkl, R, Hubac, J, Kotik, I, Monhart, Z, Burianova, H, Jerabek, O, Pisova, J, Petrova, I, Dedek, V, Honkova, M, Podrazil, P, Spinar, J, Vitovec, J, Novak, M, Lastuvka, J, Durdil, V, Antonova, P, Bockova, L, Bultas, J, Chlumsky, J, Dastychova, L, Drasnar, T, Honek, J, Horejsi, M, Hubacova, V, Janska, L, Kopeckova, I, Kratochvilova, R, Krcova, E, Labrova, R, Lindourkova, A, Lipoldova, J, Lubanda, H, Ludkova, A, Mahdalikova, L, Majernikova, M, Michalik, D, Potuznik, P, Prochazkova, E, Sulc, A, Sveceny, J, Valtova, M, Zidek, M, Zika, J, Nielsen, H, Husted, S, Hintze, U, Rasmussen, S, Bremmelgaard, A, Markenvard, J, Boerger, J, Solgaard, J, Simonsen, P, Loekkegaard, T, Bruun, M, Mertz, J, Dominguez, H, Skagen, K, Egstrup, K, Ibsen, H, Raymond, I, Bang-Hansen, T, Ellervik, C, Eriksen, E, Jensen, L, Jensen, M, Leth, M, Nygaard, A, Schou, M, Therkelsen, A, Tilma, J, Vesterager, K, Airaksinen, J, Arola, O, Koistinen, J, Nappila, H, Peltomaki, K, Rasanen, V, Vasankari, T, Galinier, M, Gottwalles, Y, Paganelli, F, Loiselet, P, Muller, J, Koujan, M, Marquand, A, Destrac, S, Piot, O, Delarche, N, Cebron, J, Boveda, S, Guenoun, M, Guedj-Meynier, D, Galley, D, Ohayon, J, Assouline, S, Zuber, M, Amarenco, P, Ellie, E, Kadouch, J, Fournier, P, Huberman, J, Lemaire, M, Rodier, G, Milandre, L, Vandamme, X, Sibon, I, Neau, J, Mahagne, M, Mielot, A, Bonnefoy, M, Churet, J, Navarre, V, Sellem, F, Monniot, G, Boyes, J, Doucet, B, Martelet, M, Obadia, D, Crousillat, B, Mouallem, J, Bearez, E, Nazeyrollas, P, Brugnaux, J, Fedorowsky, A, Casassus, F, Berneau, J, Chemin, F, Falvo, N, Perron, J, Poulard, J, Barreau, A, Beltra, C, Corrihons, E, Decarsin, N, Dubois, B, Ducasse, E, Giry, X, Kemmel, A, Ledure, S, Lemaire, N, Robin, F, Rosolin, N, Sanchez, D, Suissa, A, Koniger, G, Purr, J, Gerbaulet, U, Kellner, B, Kopf, A, Schafer, T, Zauzig, H, Riegel, P, Hohensee, H, Eissfeller, E, Eder, W, Rehling, G, Glatzel, D, Zutz, S, Heinz, G, Menke, H, Pustelnik, A, Sandow, P, Ludwig, N, Wiswedel, H, Wildenauer, W, Axthelm, C, Schwarz, T, Babyesiza, A, Stuchlik, G, Zimny, H, Kropp, M, Kahl, F, Caspar, A, Omankowsky, S, Lassig, T, Hartmann, H, Lehmann, G, Bindig, H, Hergdt, G, Reimer, D, Hauk, J, Dorsch, W, Dshabrailov, J, Michel, H, Rapp, K, Vormann, R, Mayer, P, Horstmeier, U, Eissing, V, Hey, H, Leuchtgens, H, Lilienweiss, V, Kolitsch, K, Schubert, C, Lauer, H, Buchner, T, Brauer, G, Kamin, S, Muller, K, Abdel-Qader, M, Baumbach, S, Ebert, H, Schwencke, C, Schellong, S, Bernhardt, P, Karolyi, L, Sievers, B, Haverkamp, W, Salbach, P, Rohnisch, J, Schoen, S, Erdle, W, Mueller, T, Mueller, H, Mitrovic, V, Babjakova, Z, Bergner, K, Boehme, S, Bonin, K, Buckert, D, Busch, F, Dichristin, U, Diez, S, Fleck, A, Flint, K, Floegel, H, Fritz, C, Frommhold, R, Gehre, J, Geyer, J, Grytzmann, A, Hahn, M, Helgert, K, Hubert, K, Kirchner-Volker, K, Klein, V, Kroll, D, Krueger, A, Lehmann, R, Mann, L, Maselli, A, Menken, G, Mikes, K, Mortan, H, Nasser, N, Nicolaus, D, Plauskat, A, Pomper, L, Quietzsch, A, Ravenhorst, C, Reichelt, C, Reimer, C, Schaefer, B, Scharrer, S, Schirmer, K, Schmidt, K, Schoene, R, Schulze, J, Schuppe, M, Simon, S, Sommer, S, Spranger, K, Talkenberger, A, Tauber, K, Tetlak, A, Toennishoff, T, Voelkel-Babyesiza, R, Voigts, B, Weiser, U, Wesendorf, S, Wildenauer, S, Wolf, T, Wurziger, J, Zak, J, Ziefle, S, Zincke, S, Vangel, S, Szalai, G, Merkely, B, Kancz, S, Boda, Z, Nagy, A, Laszlo, Z, Matoltsy, A, Gaszner, B, Polgar, P, Habon, T, Noori, E, Juhasz, G, Kanakaridisz, N, Szentpeteri, I, Juhasz, F, Vertes, A, Papp, A, May, Z, Ferenczi, J, Egyutt, M, Kis, E, Engelthaler, G, Szantai, G, Fulop, E, Gombos, P, Gulyas, D, Jen, P, Kiralyhazine Gyorke, E, Kovacs, M, Kovacsne Levang, S, Marianna, S, Radics, Z, Sydo, N, Szalo, R, Szilagyi, A, Sztanyik, F, Vandrus, B, Tiraferri, E, Santoro, R, Testa, S, Di Minno, G, Moia, M, Caimi, T, Martini, G, Tessitori, M, Cappelli, R, Poli, D, Quintavalla, R, Melone, F, Cosmi, F, Pizzini, A, Piseddu, G, Fanelli, R, Latella, C, Santi, R, Pancaldi, L, De Cristofaro, R, Palareti, G, De Blasio, A, Salerno Uriarte, J, Minetti, F, Pogliani, E, Lonati, L, Accogli, M, Ciampani, N, Malengo, S, Feola, M, Raisaro, A, Fattore, L, Grilli, P, Germini, F, Settimi, M, Alunni, M, Duranti, G, Tedeschi, L, Baglioni, G, Avanzino, G, Berardi, M, Pannacci, V, Giombolini, A, Nicoli, S, Scarponi, T, Allasia, B, Ricciarini, P, Nasorri, R, Argena, A, Bossolasco, P, Ronchini, P, Filippi, A, Tradati, F, Bulla, C, Donzelli, L, Foppa, L, Bottarelli, M, Tomasello, A, Mauric, A, Femiano, C, Reggio, R, Lillo, F, Mariani, A, Forcignano, F, Volpe, M, D'Avino, M, Bongiorni, M, Severi, S, Capucci, A, Lodigiani, C, Salomone, E, Serviddio, G, Tondo, C, Golino, P, Mazzone, C, Iacopino, S, Pengo, V, Galvani, M, Moretti, L, Ambrosino, P, Banfi, E, Biagioli, V, Bianchi, A, Boggian, G, Breschi, M, Brusorio, S, Calcagnoli, F, Campagna, G, Carpenedo, M, Ciabatta, C, Ciliberti, G, Cimmino, G, D'Arienzo, C, Di Gennaro, L, Fedele, M, Ferrini, P, Granzow, K, Guazzaloca, G, Guerra, F, Lo Buglio, A, Longo, S, Macellari, F, Mesolella, E, Mollica Poeta, E, Occhilupo, P, Oriana, V, Rangel, G, Salomone, L, Scaccianoce, A, Scarone, C, Segreti, L, Sottilota, G, Villani, R, Zecca, C, ten Cate, H, Ruiter, J, Klomps, H, Bongaerts, M, Pieterse, M, Guldener, C, Herrman, J, Lochorn, G, Lucassen, A, Adriaansen, H, The, S, Nierop, P, Hoogslag, P, Hermans, W, Groenemeijer, B, Terpstra, W, Buiks, C, Boersma, L, Boersma-Slootweg, M, Bosman, F, Bosschaert, M, Bruin, S, Danse, I, De Graaf, J, de Graauw, J, Debordes, M, Dols, S, Geerlings, F, Gorrebeeck, K, Jerzewski, A, Jetten, W, Kelderman, M, Kloosterman, T, Koomen, E, Krikken, J, Melman, P, Mulder, R, Pronk, A, Stallinga-de Vos, A, te Kaat, J, Tonino, P, Uppelschoten, B, van de Loo, R, van der Kley, T, van Leeuwen, G, van Putten, J, Westerman, L, Berge, E, Sirnes, P, Gjertsen, E, Hole, T, Erga, K, Hallaraker, A, Skjelvan, G, Ostrem, A, Ghezai, B, Svilaas, A, Christersson, P, Oien, T, Hoegh Henrichsen, S, Berg-Johansen, J, Otterstad, J, Antonsen, H, Ausen, K, Claussen, H, Dominguez, I, Jekthammer, A, Lensebraaten, A, Nilsen, V, O'Donovan, M, Ringdalen, K, Strand, S, Korzeniak, R, Gieroba, A, Biedrzycka, M, Ogorek, M, Wozakowska-Kaplon, B, Loboz-Grudzien, K, Kosior, J, Supinski, W, Kuzniar, J, Zaluska, R, Hiczkiewicz, J, Swiatkowska-Byczynska, L, Kucharski, L, Gruchala, M, Minc, P, Olszewski, M, Kania, G, Krzciuk, M, Lajkowski, Z, Ostrowska-Pomian, B, Lewczuk, J, Zinka, E, Karczmarczyk, A, Chmielnicka-Pruszczynska, M, Trusz-Gluza, M, Opolski, G, Bronisz, M, Glanowska, G, Ruszkowski, P, Jaworska, K, Sciborski, R, Okopien, B, Kukla, P, Wozniak-Skowerska, I, Galbas, K, Cymerman, K, Jurowiecki, J, Miekus, P, Myszka, W, Mazur, S, Lysek, R, Baszak, J, Rusicka-Piekarz, T, Raczak, G, Domanska, E, Nessler, J, Lesnik, J, Ambicka, M, Andrzejewski, D, Araminowicz, J, Barszcz, A, Bartkowiak, R, Bartnik, J, Basiak, M, Bekieszczuk, E, Bernat, L, Biedrzycki, L, Biernacka, A, Blaszczyk, D, Broton, E, Brzozowski, W, Brzustowska, M, Bzymek, R, Chmielowski, A, Chojnowski, P, Cichomski, R, Cieslak, K, Cieszynska, A, Curyllo, B, Czamara, M, Danilowicz-Szymanowicz, L, Dolecka, B, Drelich, L, Dudzik-Richter, B, Dybala, T, Dziuba, M, Faron, W, Figura-Chmielewska, M, Frankiewicz, A, Gadzinski, W, Gasior, E, Gosciniecka, B, Gutknecht, P, Guziewicz, M, Jackun-Podlesna, A, Jaguszewska, G, Jankielewicz, J, Jaremczuk-Kaczmarczyk, A, Jargiello-Baszak, M, Jarzebowski, A, Jaskulska-Niedziela, E, Jaworska-Drozdowska, M, Kabat, J, Kaczmarzyk-Radka, A, Kalin, K, Kaliszczak, R, Kiliszek, M, Klata, M, Kluczewski, M, Kobielusz-Gembala, I, Kochanska, E, Kociolek, D, Kolodzinska, A, Komlo, A, Konopka, A, Korczowska, E, Kowal, E, Kowalczyk, H, Kremis, E, Kruczyk, D, Krzesiak-Lodyga, A, Krzyzanowski, M, Kurdzielewicz, W, Kustrzycka-Kratochwil, D, Lesniewska-Krynska, D, Leszczynski, J, Lewicka, E, Lichota, E, Lip, K, Loboz-Rudnicka, M, Luka, J, Lysek-Jozefowicz, A, Machnikowska, M, Majewska, K, Mariankowski, R, Markiewicz, A, Mazur, M, Metzgier-Gumiela, A, Miedlar, E, Mielcarek, M, Neubauer-Geryk, J, Niedek, J, Niemirycz-Makurat, A, Nowak, A, Nowak, S, Opielowska-Nowak, B, Ozgowicz, M, Pawelska-Buczen, A, Pawlik-Rak, E, Piotrowicz, R, Ptaszynski, P, Raczynska, A, Rogowski, W, Romanek, J, Romaszkiewicz, R, Rostoff, P, Roszczyk, N, Rozewska-Furmanek, D, Rychta, J, Rzyczkowska, B, Sidor, A, Skalska, J, Smichura, M, Splawski, M, Staneta, P, Staniszewska, E, Starak-Marciniak, J, Stopyra-Poczatek, M, Sukiennik-Kujawa, M, Szafranski, J, Szalecki, P, Szczepanska, A, Szkrobka, W, Szuchnik, E, Szulowska, A, Szumczyk-Muszytowska, G, Szwoch, M, Traczyk, T, Troszczynska, M, Trzcinski, G, Tybura, S, Walasik, P, Wegrzynowska, M, Wesolowska, K, Wieczorek, W, Wierzbicka, A, Wilczewski, P, Wilgat-Szecowka, M, Wojewoda, P, Wojnowski, L, Wrobel, M, Zakutynska-Kowalczyk, K, Zyczynska-Szmon, M, Eltishcheva, V, Libis, R, Tereshchenko, S, Popov, S, Kamalov, G, Belenky, D, Zateyshchikova, A, Kropacheva, E, Kolesnikova, A, Nikolaev, K, Egorova, L, Khokhlov, A, Yakupov, E, Poltavskaya, M, Zateyshchikov, D, Drapkina, O, Vishnevsky, A, Barbarash, O, Miller, O, Aleksandrova, E, Chizhov, P, Sergeev, M, Shutemova, E, Mazur, E, Zrazhevskiy, K, Novikova, T, Kostenko, V, Moiseeva, Y, Polkanova, E, Sobolev, K, Rossovskaya, M, Zubeeva, G, Shapovalova, Y, Nagibovich, O, Edin, A, Agakhanyan, A, Batalov, R, Belenkova, Y, Bitakova, F, Chugunnaya, S, Dumikyan, A, Erofeeva, S, Gorbunova, E, Gorshkova, T, Gubanov, A, Gurmach, M, Ivanova, Y, Kolesova, T, Konyushenko, D, Korneeva, O, Kropova, O, Kuchuk, P, Kungurtseva, O, Kupriyanova, T, Kurylo, B, Kuvanova, M, Lebedeva, O, Lileeva, E, Machilskaya, O, Medvedeva, T, Monako, G, Motylev, I, Nagibovich, G, Novikova, E, Orlov, Y, Osmolovskaya, Y, Ovsannikova, A, Platonov, D, Rachkova, S, Sinitsina, O, Speshilova, S, Suslova, O, Ushakov, A, Volodicheva, O, Zemlianskaia, O, Zhirov, I, Zhuravleva, E, Zotova, I, Alvarez Garcia, P, Lopez Fernandez, M, Tercedor, L, Tranche Iparraguirre, S, Toran Monserrat, P, Marquez Contreras, E, Isart Rafecas, J, Motero Carrasco, J, Garcia Pavia, P, Gomez Pajuelo, C, Moro Serrano, C, Iglesias Alonso, L, Grande Ruiz, A, Merce Klein, J, Gonzalez Juanatey, J, Baron Esquivias, G, Monte Collado, I, Palacin Piquero, H, Brotons Cuixart, C, Rodriguez Morato, M, Bayo I Llibre, J, Corros Vicente, C, Vida Gutierrez, M, Epelde Gonzalo, F, Almeida Fernandez, C, Del Val Plana, N, Escriva Montserrat, E, Montero Alia, J, Barreda Gonzalez, M, Moleiro Oliva, M, Iglesias Sanmartin, J, Jimenez Gonzalez, M, Rodriguez Alvarez, M, Herreros Melenchon, J, Ripoll Vera, T, Ridocci Soriano, F, Garcia Riesco, L, Marco Macian, M, Quiles Granado, J, Jimenez Navarro, M, Cosin Sales, J, Vaquer Perez, J, Vazquez Caamano, M, Arcocha Torres, M, Marcos Gomez, G, Iniguez Romo, A, Prieto Diaz, M, Alonso, C, Alvarez, D, Alvarez, M, Amaro, M, Andere, N, Aracil Villar, J, Armitano Ochoa, R, Austria, A, Barbeira, S, Barraquer Feu, E, Bartes, A, Becerra Munoz, V, Bermudez Jimenez, F, Branjovich Tijuan, A, Cabeza Ramirez, J, Cabrera Ramos, M, Calvo Martinez, E, Campo Moreno, M, Cancho Corchado, G, Casanova Gil, M, Castillo Orive, M, Castro Fernandez, D, Cebollada del Misterio, M, Codinachs Alsina, R, Cortada Cabrera, A, Costa Pinto Prego de Faria, J, Costas, S, Cotilla Marco, M, Dachs, M, Diaz Lopez, C, Domenech Borras, A, Elorriaga Madariaga, A, Espallargas, A, Fernandez, M, Fernandez Escobar, E, Fernandez Mas, E, Ferrer, A, Fosch, J, Garcia Bermudez, M, Garcia Millan, V, Gavira Saenz, M, Gines Garcia, C, Gomez, C, Gomez Perez, Y, Gonzales Segovia, A, Gonzalez, P, Grigorian, L, Guerrero Molina, A, Del, C, Herrero Maeso, B, Hevia Rodriguez, E, Iglesias Garcia, A, Jimenez Fernandez, M, Jimeno Besa, B, Juan Salvadores, P, Lage Bouzamayor, M, Lasuncion, I, Lezcano Gort, L, Llobet Molina, M, Lopez, M, Manzanal Rey, A, Mara Guerra, J, Marcus, S, Martin Vila, A, Martinez Mena, M, Mazon, P, Mendez Zurita, F, Millan, G, Molina, M, Montero Alia, P, Montes, D, Moure Gonzalez, M, Munoz Munoz, R, Negrete Palma, A, Orellana Figueroa, H, Ortega, V, Ortiz Cortes, C, Otero Tomera, D, Palomo Merchan, N, Pareja Ibar, I, Pena Garcia, E, Pereda Armayor, M, Perez Carasa, M, Prieto, I, Quintern, V, Renom, R, Rincon Diaz, L, Rios, V, Riquelme Sola, L, Rivera, R, Robiro Robiro, X, Roca, M, Roca Saumell, C, Rodrigo, C, Rodriguez, E, Rodriguez Garcia, M, Saez Jimenez, S, Sanchez Calderon, P, Sanchez Mendez, L, Sanchez Parra, S, Santolaya, C, Senan Sanz, M, Seoane Blanco, A, Serralvo, E, Sierra, N, Simon Valero, C, Sorribes Lopez, J, Teixido Fontanillas, M, Terns Riera, M, Tobajas, G, Torres, C, Torres Marques, J, Ubeda Pastor, M, Wirdby, A, Linden, J, Henriksson, K, Elmersson, M, Egilsson, A, Borjesson, U, Svard, G, Liu, B, Lindh, A, Olsson, L, Gustavsson, M, Andersson, L, Benson, L, Bothin, C, Hajimirsadeghi, A, Kadir, K, Ericsson, M, Ohlsson, A, Lindvall, H, Svensson, P, Thorne, K, Handel, H, Platonov, P, Eriksson, B, Timberg, I, Romberg, K, Crisby, M, Karlsson, J, Jensen, S, Andersson, A, Malmqvist, L, Martinsson, B, Bernsten, F, Engdahl, J, Thulin, J, Hot-Bjelac, A, Stalby, P, Aaroe, H, Ahbeck, E, Ahlmark, H, Al-Khalili, F, Bonkowski, G, Dzeletovic, S, Ekstrand, A, Eriksson, G, Floren, K, Grassjo, C, Hahn, S, Jaensson, P, Jansson, B, Jansson, J, Kangert, R, Koch, A, Kusiak, D, Lettenstrom, A, Lindberg, A, Lindholm, C, Mannermyr, A, Mansson, K, Millborg, M, Nilsson, C, Ohlin, A, Olofsson, A, Osberg, A, Pedersen, A, Risbecker, K, Rosenberg, K, Samuelsson, J, Shayesteh, M, Skoglund, K, Stjernberg, M, Thorsen, C, Beer, J, Debrunner, J, Amstutz, D, Bruegger, J, Elise, G, Grau, A, Guinand, A, Henriette, I, Saga, E, Winnik, S, Rudyk, I, Tseluyko, V, Karpenko, O, Zhurba, S, Kraiz, I, Kupnovytska, I, Serediuk, N, Mostovoy, Y, Ushakov, O, Koval, O, Kovalskyi, I, Svyshchenko, Y, Sychov, O, Stanislavchuk, M, Kraydashenko, O, Yagensky, A, Tykhonova, S, Fushtey, I, Belegai, R, Berko, G, Burdeuna, L, Chabanna, O, Daniuk, I, Ivanov, A, Kamenska, E, Kaplan, P, Khyzhnyak, O, Kizim, S, Matova, O, Medentseva, O, Mochonyi, V, Mospan, M, Nemtsova, V, Ovdiienko, T, Palamarchuk, O, Pavelko, M, Petrovskyy, R, Plevak, D, Proshak, O, Pyvovar, S, Rasputina, L, Romanenko, O, Romanova, O, Sapatyi, A, Shumakov, O, Stets, R, Todoriuk, L, Varenov, V, Chauhan, N, Goodwin, D, Saunders, P, Evans, R, Leese, J, Jhittay, P, Ross, A, Kainth, M, Pickavance, G, Mcdonnell, J, Williams, A, Gooding, T, Wagner, H, Suryani, S, Singal, A, Sircar, S, Bilas, R, Hutchinson, P, Wakeman, A, Stokes, M, Paul, N, Aziz, M, Ramesh, C, Wilson, P, Franklin, S, Fairhead, S, Thompson, J, St Joseph, V, Taylor, G, Tragen, D, Seamark, D, Paul, C, Richardson, M, Jefferies, A, Sharp, H, Jones, H, Giles, C, Page, M, Oginni, O, Aldegather, J, Wetherwell, S, Lumb, W, Evans, P, Scouller, F, Macey, N, Stipp, Y, West, R, Thurston, S, Wadeson, P, Matthews, J, Pandya, P, Gallagher, A, Railton, T, Sinha, B, Russell, D, Davies, J, Ainsworth, P, Jones, C, Weeks, P, Eden, J, Kernick, D, Murdoch, W, Lumley, L, Patel, R, Wong, S, Saigol, M, Ladha, K, Douglas, K, Cumberlidge, D, Bradshaw, C, Van Zon, G, Jones, K, Thomas, M, Watson, E, Sarai, B, Ahmad, N, Willcock, W, Cairns, J, Sathananthan, S, de Kare-Silver, N, Gilliland, A, Strieder, E, Howitt, A, Vishwanathan, B, Bird, N, Gray, D, Clark, M, Bisatt, J, Litchfield, J, Fisher, E, Fooks, T, Kelsall, A, Alborough, E, Wakeling, J, Parfitt, M, Milne, K, Rogers, S, Priyadharshan, R, Oliver, J, Davies, E, Abushal, S, Jacobs, M, Hutton, C, Walls, N, Thompson, R, Chigbo, C, Zaidi, S, Howard, M, Butter, K, Barrow, S, Little, H, Haq, I, Gibbons, L, Glencross, S, Mcleod, A, Poland, K, Mulholland, C, Warke, A, Conn, P, Burns, G, Smith, R, Lowe, S, Kamath, R, Dau, H, Webster, J, Hodgins, I, Vercoe, S, Roome, P, Pinnock, H, Patel, J, Ali, A, Hart, N, Davies, R, Stuart, E, Neden, C, Danielsen, M, Heath, R, Sharma, P, Galloway, S, Hawkins, C, Oliver, R, Aylward, M, Mannion, S, Braddick, M, Edwards, D, Rothwell, A, Sabir, A, Choudhary, F, Khalaque, S, Wilson, A, Peters, S, Coulson, W, Roberts, N, Heer, A, Coates, S, Ward, B, Jackson, D, Walton, S, Shepherd, D, Sterry, M, Wong, T, Boon, M, Bunney, R, Haria-Shah, R, Baron, R, Davies, S, Schatzberger, T, Hargreaves, N, Stephenson, T, Batson, R, Lucraft, L, Myhill, T, Estifano, S, Geatch, D, Wilkinson, J, Veale, R, Forshaw, K, Davies, T, Zaman, K, Vinson, P, Liley, C, Bandrapalli, M, Mcginty, P, Wastling, R, Mceleny, P, Beattie, A, Cooke, P, Wong, M, Gunasegaram, J, Pugsley, M, Ahmad, S, A'Court, C, Ayers, J, Bennett, J, Cartwright, S, Dobson, S, Dooldeniya, C, Flynn, A, Fox, R, Goram, J, Halpin, A, Hay, A, Jacobs, P, Jeffers, L, Lomax, L, Munro, I, Muvva, R, Nadaph, M, Powell, K, Randfield, S, Redpath, D, Reed, R, Rickenbach, M, Rogers, G, Seamark, C, Shewring, J, Simmons, P, Simper, H, Stoddart, H, Sword, A, Thomas, N, Thomson, A, Blenkhorn, A, Singh, B, Van Gaal, W, Abhayaratna, W, Lehman, R, Roberts-Thomson, P, Kilian, J, Coulshed, D, Catanchin, A, Colquhoun, D, Kiat, H, Eccleston, D, French, J, Zimmett, L, Ayres, B, Phan, T, Blombery, P, Crimmins, D, O'Donnell, D, Choi, A, Astridge, P, Arstall, M, Jepson, N, Binnekamp, M, Lee, A, Rogers, J, Starmer, G, Carroll, P, Faunt, J, Aggarwala, A, Barry, L, Batta, C, Beveridge, R, Black, A, Bonner, M, Boys, J, Buckley, E, Campo, M, Carlton, L, Connelly, A, Conway, B, Cresp, D, Dimitri, H, Dixon, S, Dolman, M, Duroux, M, Eskandari, M, Eslick, R, Ferreira-Jardim, A, Fetahovic, T, Fitzpatrick, D, Geraghty, R, Gibbs, J, Grabek, T, Modi, M, Hayes, K, Hegde, M, Hesketh, L, Hoffmann, B, Johnson, K, Juergens, C, Kassam, I, Lawlor, V, Lehman, M, Lehman, S, Leung, D, Mackay, S, Mackenzie, M, Mccarthy, C, Mcintosh, C, Mckeon, L, Morrison, H, Mussap, C, Myers, J, Nagalingam, V, Oldfield, G, O'May, V, Palmer, J, Parsons, L, Patching, K, Patching, T, Paul, V, Plotz, M, Preston, S, Rashad, H, Ratcliffe, M, Raynes, S, Rose, J, Sanders, L, Seremetkoska, M, Setio, H, Shone, S, Shrestha, P, Singh, C, Singleton, C, Stoyanov, N, Sutcliffe, S, Swaraj, K, Tarrant, J, Thompson, S, Tsay, I, Vorster, M, Waldman, A, Wallis, L, Wilford, E, Wong, K, Luton, R, Gupta, M, Pandey, A, Cheung, S, Leader, R, Beaudry, P, Ayala-Paredes, F, Berlingieri, J, Heath, J, Poirier, G, Du Preez, M, Nadeau, R, Dresser, G, Dhillon, R, Hruczkowski, T, Schweitzer, B, Coutu, B, Angaran, P, Macdonald, P, Vizel, S, Fikry, S, Parkash, R, Lavoie, A, Cha, J, Ramjattan, B, Bonet, J, Ahmad, K, Aro, L, Aves, T, Beaudry, K, Bergeron, C, Bigcanoe, J, Bignell, N, Breakwell, L, Burke, E, Carroll, L, Clarke, B, Cleveland, T, Daheb, S, Dehghani, P, Denis, I, Djaidani, Z, Dorian, P, Douglass, S, Dunnigan, J, Ewert, A, Farquhar, D, Fearon, A, Ferleyko, L, Fournier, D, Fox, B, Grenier, M, Gulliver, W, Haveman, K, Hines, C, Hines, K, Jackson, A, Jean, C, Jethoo, G, Kahlon, R, Kelly, S, Kim, R, Korley, V, Kornder, J, Kwan, L, Largy, J, Lewis, C, Lewis, S, Mangat, I, Moor, R, Navratil, J, Neas, I, Otis, J, Otis, R, Pandey, M, Petrie, F, Pinter, A, Raines, M, Roberts, P, Robinson, M, Sas, G, Schulman, S, Snell, L, Spearson, S, Stevenson, J, Trahey, T, Wright, D, Abd El-Aziz, A, Abou Seif, S, El Din, M, El Etriby, S, Elbahry, A, El-Etreby, A, Elkhadem, M, Katta, A, Khairy, T, Mowafy, A, Nawar, M, Ohanissian, A, Reda, A, Reda, M, Salem, H, Sami, N, Samir, S, Setiha, M, Sobhy, M, Soliman, A, Taha, N, Tawfik, M, Zaatout, E, Kettles, D, Bayat, J, Siebert, H, Horak, A, Kelfkens, Y, Garda, R, Pillay, T, Guerra, M, van Zyl, L, Theron, H, Murray, A, Louw, R, Greyling, D, Mntla, P, Ueckermann, V, Loghdey, R, Ismail, S, Ahmed, F, Engelbrecht, J, Ramdass, A, Maharajh, S, Oosthuysen, W, Angel, G, Bester, C, Booysen, M, Boshoff, C, Cannon, C, Cassimjee, S, Chami, C, Conway, G, Davids, A, de Meyer, L, Du Plessis, G, Ellis, T, Henley, L, Karsten, M, Loyd, E, Marks, J, Mavhusa, L, Mostert, M, Page, A, Rikhotso, L, Salie, M, Sasto, J, Shaik, F, Skein, A, Smith, L, Tarr, G, Tau, T, van Zyl, F, Yousef, G, Agrawal, A, Nathani, M, Ibrahim, M, Esheiba, E, Singh, R, Naguib, A, Abu-Mahfouz, M, Al Omairi, M, Al Naeemi, A, Maruthanayagam, R, Bazargani, N, Wassef, A, Gupta, R, Khan, M, Subbaraman, B, Abdul, A, Al Mulla, A, El Bardisy, S, Haridas, P, Jadhav, S, Magdaluyo, K, Makdad, M, Maqsood, I, Mohamed, R, Sharma, N, Sharma, R, Thanzeel, M, Canosa, R, Rama, P, Blumberg, E, Garcia, J, Mullen, P, Wilson, V, Quick, A, Ferrick, K, Kutayli, W, Cox, M, Franco, M, Falkowski, S, Mendelson, R, Williams, M, Miller, S, Beach, S, Alfieri, A, Gutowski, T, Haque, I, Reddy, R, Ahmed, W, Delafontaine, P, Diercks, D, Theodoro, D, Remmel, K, Alberts, M, Ison, R, Noveck, H, Duffy, P, Pitta, S, Nishijima, D, Treasure, C, Asafu-Adjaye, N, Ball, K, Bartlett, M, Bentley, M, Bowers, S, Brown, A, Browne, A, Cameron-Watts, J, Canova, M, Cassidy, D, Cervellione, K, Congal, S, Depauw, J, Dickerson, A, Eley, M, Evans, L, Felpel, S, Ferdinand, K, Fielder, D, Gentry, P, Haideri, A, Hakimi, F, Harbour, T, Hartranft, E, Hawkins, B, Headlee, M, Henson, L, Herrick, C, Hicks, T, Jasinski, S, Jones, A, Jones, L, Jones, P, Karl, S, Keeling, M, Kerr, J, Knowles, P, Langdon, J, Lay, M, Lee, J, Lincoln, T, Malone, E, Merliss, A, Merritt, D, Minardo, J, Mooso, B, Orosco, C, Palumbo, V, Parker, M, Parrott, T, Paserchia, S, Pearl, G, Peterson, J, Pickelsimer, N, Purcell, T, Raynor, J, Raziano, S, Richard, C, Richardson, T, Robertson, C, Sage, A, Sanghera, T, Shaw, P, Shoemaker, J, Smith, K, Stephanie, B, Thatcher, A, Theobald, H, Thompson, N, Treasure, L, Tripti, T, Verdi, C, Worthy, V, Sawhney, Jitendra PS., Kothiwale, Veerappa A., Bisne, Vika, Durgaprasad, Rajashekhar, Jadhav, Praveen, Chopda, Manoj, Vanajakshamma, Velam, Meena, Ramdhan, Vijayaraghavan, Govindan, Chawla, Kamaldeep, Allu, Jagan, Pieper, Karen S., John Camm, A., Kakkar, Ajay K., Bassand, Jean-Pierre, Fitzmaurice, David A., Goldhaber, Samuel Z., Goto, Shinya, Haas, Sylvia, Hacke, Werner, Mantovani, Lorenzo G., Misselwitz, Frank, Turpie, Alexander G. G., van Eickels, Martin, Verheugt, Freek W. A., Kayani, Gloria, Fox, Keith A. A., Gersh, Bernard J., Luciardi, Hector Luca, Gibbs, Harry, Brodmann, Marianne, Cools, Frank, Barretto, Antonio Carlos Pereira, Connolly, Stuart J., Spyropoulos, Alex, Eikelboom, John, Corbalan, Ramon, Hu, Dayi, Jansky, Petr, Nielsen, Jørn Dalsgaard, Ragy, Hany, Raatikainen, Pekka, Le Heuzey, Jean-Yve, Darius, Harald, Keltai, Matya, Kakkar, Sanjay, Sawhney, Jitendra Pal Singh, Agnelli, Giancarlo, Ambrosio, Giuseppe, Koretsune, Yukihiro, Sánchez Díaz, Carlos Jerje, Ten Cate, Hugo, Atar, Dan, Stepinska, Janina, Panchenko, Elizaveta, Lim, Toon Wei, Jacobson, Barry, Oh, Seil, Viñolas, Xavier, Rosenqvist, Marten, Steffel, Jan, Angchaisuksiri, Pantep, Oto, Ali, Parkhomenko, Alex, Al Mahmeed, Wael, Fitzmaurice, David, Hu, D. Y., Chen, K. N., Zhao, Y. S., Zhang, H. Q., Chen, J. Z., Cao, S. P., Wang, D. W., Yang, Y. J., Li, W. H., Yin, Y. H., Tao, G. Z., Yang, P., Chen, Y. M., He, S. H., Wang, Ying, Wang, Yong, Fu, G. S., Li, X., Wu, T. G., Cheng, X. S., Yan, X. W., Zhao, R. P., Chen, M. S., Xiong, L. G., Chen, P., Jiao, Y., Guo, Y., Xue, L., Wang, F. Z., Li, H., Yang, Z. M., Bai, C. L., Chen, J., Chen, J. Y., Chen, X., Feng, S., Fu, Q. H., Gao, X. J., Guo, W. N., He, R. H., He, X. A., Hu, X. S., Huang, X. F., Li, B., Li, J., Li, L., Li, Y. H., Liu, T. T., Liu, W. L., Liu, Y. Y., Lu, Z. C., Luo, X. L., Ma, T. Y., Peng, J. Q., Sheng, X., Shi, X. J., Sun, Y. H., Tian, G., Wang, K., Wang, L., Wu, R. N., Xie, Q., Xu, R. Y., Yang, J. S., Yang, L. L., Yang, Q., Ye, Y., Yu, H. Y., Yu, J. H., Yu, T., Zhai, H., Zhan, Q., Zhang, G. S., Zhang, Q., Zhang, R., Zhang, Y., Zheng, W. Y., Zhou, B., Zhou, Z. H., Zhu, X. Y., Kakkar, S., Sawhney, J. P. S., Jadhav, P., Durgaprasad, R., Ravi Shankar, A. G., Rajput, R. K., Bhargava, K., Sarma, R., Srinivas, A., Roy, D., Nagamalesh, U. M., Chopda, M., Kishore, R., Kulkarni, G., Chandwani, P., Pothiwala, R. A., Padinhare Purayil, M., Shah, S., Chawla, K., Kothiwale, V. A., Raghuraman, B., Vijayaraghavan, G., Vijan, V. M., Bantwal, G., Bisne, V., Khan, A., Gupta, J. B., Kumar, S., Jain, D., Abraham, S., Adak, D., Barai, A., Begum, H., Bhattacharjee, P., Dargude, M., Davies, D., Deshpande, B., Dhakrao, P., Dhyani, V., Duhan, S., Earath, M., Ganatra, A., Giradkar, S., Jain, V., Karthikeyan, R., Kasala, L., Kaur, S., Krishnappa, S., Lawande, A., Lokesh, B., Madarkar, N., Meena, R., More, P., Naik, D., Prashanth, K., Rao, M., Rao, N. M., Sadhu, N., Shah, D., Sharma, M., Shiva, P., Singhal, S., Suresh, S., Vanajakshamma, V., Panse, S. G., Koretsune, Y., Kanamori, S., Yamamoto, K., Kumagai, K., Katsuda, Y., Sadamatsu, K., Toyota, F., Mizuno, Y., Misumi, I., Noguchi, H., Ando, S., Suetsugu, T., Minamoto, M., Oda, Hiroshi, Shiraishi, K., Adachi, S., Chiba, K., Norita, H., Tsuruta, M., Koyanagi, T., Ando, H., Higashi, T., Okada, K., Azakami, S., Komaki, S., Kumeda, K., Murayama, T., Matsumura, J., Oba, Y., Sonoda, R., Goto, K., Minoda, K., Haraguchi, Y., Suefuji, H., Miyagi, H., Kato, H., Nakamura, Tadashi, Nakamura, Tsugihiro, Nandate, H., Zaitsu, R., Fujiura, Yoshihisa, Yoshimura, A., Numata, H., Ogawa, J., Tatematsu, H., Kamogawa, Y., Murakami, K., Wakasa, Y., Yamasawa, M., Maekawa, H., Abe, S., Kihara, H., Tsunoda, S., Saito, Katsumi, Saito, Kazuyuki, Fudo, T., Obunai, K., Tachibana, H., Oba, I., Kuwahata, T., Higa, S., Gushiken, M., Eto, T., Yoshida, H., Ikeda, D., Fujiura, Yoshitake, Ishizawa, M., Nakatsuka, M., Murata, K., Ogurusu, C., Shimoyama, M., Akutsu, M., Takamura, I., Hoshino, F., Yokota, N., Iwao, T., Tsuchida, K., Takeuchi, M., Hatori, Y., Kitami, Y., Nakamura, Yoichi, Oyama, R., Ageta, M., Oda, Hiroyuki, Go, Y., Mishima, K., Unoki, T., Morii, S., Shiga, Yuhei, Sumi, H., Nagatomo, T., Sanno, K., Fujisawa, K., Atsuchi, Y., Nagoshi, T., Seto, T., Tabuchi, T., Kameko, M., Nii, K., Oshiro, K., Takezawa, H., Nagano, S., Miyamoto, N., Iwaki, M., Nakamura, Yuichiro, Fujii, M., Okawa, M., Abe, Masahiko, Abe, Masatake, Abe, Mitsunori, Saito, T., Mito, T., Nagao, K., Minami, J., Mita, T., Sakuma, I., Taguchi, T., Marusaki, S., Doi, H., Tanaka, M., Fujito, T., Matsuta, M., Kusumoto, T., Kakinoki, S., Ashida, K., Yoshizawa, N., Agata, J., Arasaki, O., Manita, M., Ikemura, M., Fukuoka, S., Murakami, H., Matsukawa, S., Hata, Y., Taniguchi, T., Ko, T., Kubo, H., Imamaki, M., Akiyama, M., Inagaki, M., Odakura, H., Ueda, T., Katsube, Y., Nakata, A., Watanabe, H., Techigawara, M., Igarashi, M., Taga, K., Kimura, T., Tomimoto, S., Shibuya, M., Nakano, M., Ito, K., Seo, T., Hiramitsu, S., Hosokawa, H., Hoshiai, M., Hibino, M., Miyagawa, K., Horie, Hajime, Sugishita, N., Shiga, Yukio, Soma, A., Neya, K., Yoshida, Tetsuro, Yoshida, Tomoki, Mizuguchi, M., Ishiguro, M., Minagawa, T., Wada, M., Mukawa, H., Okuda, F., Nagasaka, S., Abe, Y., Adachi, Sen, Adachi, Susumu, Adachi, T., Akahane, K., Amano, T., Aoki, K., Aoyama, T., Arai, H., Arima, S., Arino, T., Asano, H., Asano, T., Azuma, J., Baba, T., Betsuyaku, T., Chibana, H., Date, H., Doiuchi, J., Emura, Y., Endo, M., Fujii, Y., Fujiki, R., Fujisawa, A., Fujisawa, Y., Fukuda, T., Fukui, T., Furukawa, N., Furukawa, T., Furumoto, W., Goto, T., Hamaoka, M., Hanazono, N., Hasegawa, K., Hatsuno, T., Hayashi, Y., Higuchi, K., Hirasawa, K., Hirayama, H., Hirose, M., Hirota, S., Honda, M., Horie, Hideki, Ido, T., Iiji, O., Ikeda, H., Ikeda, K., Ikeoka, K., Imaizumi, M., Inaba, H., Inoue, T., Iseki, F., Ishihara, A., Ishioka, N., Ito, N., Iwase, T., Kakuda, H., Kamata, J., Kanai, H., Kanda, H., Kaneko, M., Kano, H., Kasai, T., Kato, T., Kato, Y., Kawada, Y., Kawai, K., Kawakami, K., Kawakami, S., Kawamoto, T., Kawano, S., Kim, J., Kira, T., Kitazawa, H., Kitazumi, H., Kito, T., Kobayashi, T., Koeda, T., Kojima, J., Komatsu, H., Komatsu, I., Koshibu, Y., Kotani, T., Kozuka, T., Kumai, Y., Kumazaki, T., Maeda, I., Maeda, K., Maruyama, Y., Matsui, S., Matsushita, K., Matsuura, Y., Mineoi, K., Mitsuhashi, H., Miura, N., Miyaguchi, S., Miyajima, S., Miyamoto, H., Miyashita, A., Miyata, S., Mizuguchi, I., Mizuno, A., Mori, T., Moriai, O., Morishita, K., Murai, O., Nagai, Sho, Nagai, Shunichi, Nagata, E., Nagata, H., Nakagomi, A., Nakahara, S., Nakamura, M., Nakamura, R., Nakanishi, N., Nakayama, T., Nakazato, R., Nanke, T., Nariyama, J., Niijima, Y., Niinuma, H., Nishida, Y., Nishihata, Y., Nishino, K., Nishioka, H., Nishizawa, K., Niwa, I., Nomura, K., Nomura, S., Nozoe, M., Ogawa, T., Ohara, N., Okada, M., Okamoto, K., Okita, H., Okuyama, M., Ono, H., Ono, T., Onuki Pearce, Y., Oriso, S., Ota, A., Otaki, E., Saito, Y., Sakai, H., Sakamoto, N., Sakamoto, Y., Samejima, Y., Sasagawa, Y., Sasaguri, H., Sasaki, A., Sasaki, T., Sato, Kazuki, Sato, Kiyoharu, Sawano, M., Seki, S., Sekine, Y., Seta, Y., Sezaki, K., Shibata, N., Shiina, Y., Shimono, H., Shimoyama, Y., Shindo, T., Shinohara, H., Shinohe, R., Shinozuka, T., Shirai, T., Shiraiwa, T., Shozawa, Y., Suga, T., Sugimoto, C., Suzuki, Kazuo, Suzuki, Keita, Suzuki, Shu, Suzuki, Shunji, Suzuki, Susumu, Suzuki, Y., Tada, M., Taguchi, A., Takagi, T., Takagi, Y., Takahashi, K., Takahashi, S., Takai, H., Takanaka, C., Take, S., Takeda, H., Takei, K., Takenaka, K., Tana, T., Tanabe, G., Taya, K., Teragawa, H., Tohyo, S., Toru, S., Tsuchiya, Y., Tsuji, T., Tsuzaki, K., Uchiyama, H., Ueda, O., Ueyama, Y., Wakaki, N., Wakiyama, T., Washizuka, T., Watanabe, M., Yamada, T., Yamagishi, T., Yamaguchi, H., Yamamoto, Kenichi, Yamamoto, Kentaro, Yamamoto, Kunihiko, Yamamoto, T., Yamaura, M., Yamazoe, M., Yasui, K., Yokoyama, Y., Yoshida, K., Lim, T. W., Ching, C. K., Foo, C. G., Chow, J. H., Chen, D. D., Jaufeerally, F. R., Lee, Y. M., Lim, G., Lim, W. T., Thng, S., Yap, S. Y., Yeo, C., Oh, S., Pak, H. N., Kim, J. -B., Kim, J. H., Jang, S. -W., Kim, D. H., Ryu, D. R., Park, S. W., Kim, D. -K., Choi, D. J., Oh, Y. S., Cho, M. -C., Kim, S. -H., Jeon, H. -K., Shin, D. -G., Park, J. S., Park, H. K., Han, S. -J., Sung, J. H., Cho, J. -G., Nam, G. -B., On, Y. K., Lim, H. E., Kwak, J. J., Cha, T. -J., Hong, T. J., Park, S. H., Yoon, J. H., Kim, N. -H., Kim, K. -S., Jung, B. C., Hwang, G. -S., Kim, C. -J., Kim, D. B., Ahn, J. J., An, H. J., Bae, H., Baek, A. L., Chi, W. J., Choi, E. A., Choi, E. H., Choi, H. K., Choi, H. S., Han, S., Heo, E. S., Her, K. O., Hwang, S. W., Jang, E. M., Jang, H. -S., Jang, S., Jeon, H. -G., Jeon, S. R., Jeon, Y. R., Jeong, H. K., Jung, I. -A., Kim, Hyeon Jeong, Kim, Hyun Ju, Kim, Ji Seon, Kim, Jung Sook, Kim, J. A., Kim, K. T., Kim, M. S., Kim, Sang Hee, Kim, Sang Hyun, Kim, Y. -I., Lee, C. S., Lee, E. H., Lee, G. H., Lee, H. Y., Lee, H. -Y., Lee, K. H., Lee, K. R., Lee, M. S., Lee, M. -Y., Lee, R. W., Lee, S. E., Lee, S. H., Lee, S., Lee, W. Y., Noh, I. K., Park, A. R., Park, B. R., Park, H. N., Park, J. H., Park, M., Park, Y., Seo, S. -Y., Shim, J., Sim, J. H., Sohn, Y. M., Son, W. S., Son, Y. S., Song, H. J., Wi, H. K., Woo, J. J., Ye, S., Yim, K. H., Yoo, K. M., Yoon, E. J., Yun, S. Y., Angchaisuksiri, P., Chawanadelert, S., Mongkolwongroj, P., Kanokphatcharakun, K., Cheewatanakornkul, S., Laksomya, T., Pattanaprichakul, S., Chantrarat, T., Rungaramsin, S., Silaruks, S., Wongcharoen, W., Siriwattana, K., Likittanasombat, K., Katekangplu, P., Boonyapisit, W., Cholsaringkarl, D., Chatlaong, B., Chattranukulchai, P., Santanakorn, Y., Hutayanon, P., Khunrong, P., Bunyapipat, T., Jai-Aue, S., Kaewsuwanna, P., Bamungpong, P., Gunaparn, S., Hongsuppinyo, S., Inphontan, R., Khattaroek, R., Khunkong, K., Kitmapawanont, U., Kongsin, C., Naratreekoon, B., Ninwaranon, S., Phangyota, J., Phrommintikul, A., Phunpinyosak, P., Pongmorakot, K., Poomiphol, S., Pornnimitthum, N., Pumprueg, S., Ratchasikaew, S., Sanit, K., Sawanyawisuth, K., Silaruks, B., Sirichai, R., Sriwichian, A., Suebjaksing, W., Sukklad, P., Suttana, T., Tangsirira, A., Thangpet, O., Tiyanon, W., Vorasettakarnkij, Y., Wisaratapong, T., Wongtheptien, W., Wutthimanop, A., Yawila, S., Oto, A., Altun, A., Ozdogru, I., Ozdemir, K., Yilmaz, O., Aydinlar, A., Yilmaz, M. B., Yeter, E., Ongen, Z., Cayli, M., Pekdemir, H., Ozdemir, M., Sucu, M., Sayin, T., Demir, M., Yorgun, H., Ersanli, M., Okuyan, E., Aras, D., Abdelrahman, H., Aktas, O., Alpay, D., Aras, F., Bireciklioglu, M. F., Budeyri, S., Buyukpapuc, M., Caliskan, S., Esen, M., Felekoglu, M. A., Genc, D., Ikitimur, B., Karaayvaz, E. B., Kılıç Karataş, S., Okutucu, S., Ozcelik, E., Quisi, A., Sag, H., Sahiner, L., Sayin, B. Y., Seker, T., Uzun Alkan, D., Yildirim, E., Yildirim, R., Yilmaz, F., Yuksekdag, V., Luciardi, H. L., Vensentini, N., Ingaramo, A. C., Sambadaro, G. A., Fernandez Caputi, V., Berman, S. G., Dragotto, P., Kleiban, A. J., Centurion, N., Giacomi, G., Ahuad Guerrero, R. A., Conde, D., Zapata, G., Di Paola, L. A., Ramos, J. L., Dran, R. D., Egido, J., Fernandez, A. A., Fosco, M. J., Sassone, S., Sinisi, V. A., Cartasegna, L. R., Berli, M. A., Gomez Vilamajo, O. A., Ferroni, F., Alaguibe, E. D., Alvarez D'Amelio, A., Arabetti, C., Arias, L., Belardi, J. A., Bergesio, L., Berli, F., Berli, M., Borchowiec, S., Buzzetti, C., Cabrini, R., Campisi, V., Cappi, A. L., Carrizo, R., Colombo Berra, F., Costabel, J. P., Costamagna, O. J. A., Damonte, A. A., De Urquiza, I. N., Diez, F., Edén, M. F., Fanuele, M., Fernandez Voena, F., Foa Torres, M., Funosas, C., Giacomi, M. P., Gimenez, C. H., Gurfinkel, E. P., de L. M. Had, M., Hansen, V., Hrabar, A. D., Ingratta, M., Lopez, A., Maehara, G., Maffei, L., Martinelli, A., Martinelli, C., Matkovich, J., Mautner, B., Meirino, A., Munguia, R., Navarro, A., Novas, V., Perez Prados, G., Pontoriero, J., Potito, R. N., Ricotti, C., Rodriguez, M. A., Rolandi, F., Said Palladino, M. E., Salinger, M., Sanziani, L. S., Schygiel, P. O., Sossich, A., Tinto, J. F., Tonelli, L., Tufare, A. L., Vallejo, M., Yunis, M. E., Zillo, M., Zurbrigk, F. J., Barretto, A. C. P., Sobral Filho, D. C., Jaber, J., Armaganijan, D., Faria Neto, J., Steffens, A., Kunz Sebba Barroso de Souza, W., de Souza Neto, J. D., Ribeiro, J. M., Silveira Teixeira, M., Ferreira Rossi, P. R., Pires, L., Moreira, D., Moura Jorge, J. C., Menezes Lorga Filho, A., Bodanese, L. C., Westerlund Montera, M., Del Carlo, C. H., Da Rocha Rodrigues, T., Alves da Costa, F. A., Lopes, A., Lopes, R., Araújo, G. R., Fernandes Manenti, E. R., Kerr Saraiva, J. F., Ferreira Braga, J. C., Negri, A., Souto, L., Moncada, C., Bertolim Precoma, D., Roquette, F., Reis, G., Ramos Filho, R. A., Lanna Figueiredo, E., Vieira Botelho, R., Munhoz da Fontoura Tavares, C., Costantini Frack, C. R., Abdalla Saad, J., Finimundi, H. C., Pisani, C., Chemello, D., Pereira Martins, M., Broilo França, C. C., Alban, F., Aranha Rosito, G. B., de Moura Xavier Moraes Junior, J. B., Tumelero, R. T., Nigro Maia, L., Simões de Almeida, R., do Carmo Borges, N. C., Gomes Ferreira, L. G., Agliardi, P., Alves de Oliveira Gomes, J., Araujo, V., Arruda Nakazone, M., Barbosa, T., Barroso, S., Belisario Falchetto, E., Bellotti Lopes, H., Benez Teixeira Lemos, M. A., Biazus, G., Borges Queiroz, L., Camazzola, F. E., Caporale, M., Cardoso Boscato, S., Chieza, F., Chokr, M. O., Clemente Mingireanov, R., Codonho Góes, N., Correa, C., Costa, M., Costantini Ortiz, C., da Silva, L. S., da Silva Paulitsch, F., da Silveira, J. A., Daros, E., de Araújo, G. R., Del Monaco, M. I., Dias, C., Dias, M. A., Drummond Wainstein, A. P., Ely Pizzato, P., Esteves, D. C., Fabri, P., Félix Lorenzato Fonseca, T., Fernandes, E., Fonseca, C., Frack Costantini, C. R., Franchin Ferraz, R., Freire, F., Gottardo, P., Guanaes, D., Guizzardi, S., Hettwer Magedanz, E., Igansi, F., Jannuzzi, F., Junior, G., Komar, D., Lino, E. G., Lopes, D., Lourenço da Silva Júnior, O., Lustosa, E., Macagnan, A. P., Marinho, M. C., Mazzoni, M., Melo, G., Mortari, L., Mouco, O. M. C. C., Nanzer Vital, C., Ormundo, C., Oss Emmer, S., Palmegiani, E., Pavani, R., Pereira, L., Pereira, V. L., Perreira, R., Poletti, S., Quaia Fortunato, S. C., Queirantes, C., Ramos Pereira, N., Rech, R. L., Ribeiro, S., Rodrigues, A., Roesch, H., Ruaro Reichert, T., Santos, D., Santos, I., Santos, M., Seroqui, M. V., Silva, S., Soares, L., Spolaor, L., Stoll, C., Toazza Duda, N., Trama, L., Unterkircher, B., Valois, M. V., Vargas, T., Viana, T., Vicente, C., Vidal Armaganijan, L., Vieira Homem, R., Vieira Torres, L. G., Vila Boas, L., Villaça Guimarães Filho, F., Corbalan, R., Eggers, G., Bugueño Gutiérrez, C., Arriagada, G., Potthoff Cardenas, S., Stockins Fernandez, B. A. J., Conejeros, C., Houzvic, C., Marin Cuevas, P., Montecinos, H., Forero, A., Lanas, F., Larico Gómez, M., Charme Vilches, G., Rey, C., Astudillo, C., Aguilar, J., Campisto, Y., Lara, C., Molina, E., Munoz Oyarzon, J., Olguin, V., Vergara, M., Villan, C., Sánchez Díaz, C. J., Illescas Diaz, J., Leal Cantú, R., Ramos Zavala, M. G., Cabrera Jardines, R., Espinola Zavaleta, N., Villarreal Umaña, S., López Rosas, E., Llamas Esperón, G., Pozas, G., Cardona Muñoz, E., Matadamas Hernández, N., Leyva Rendón, A., García Hernández, N., de los Ríos Ibarra, M., Virgen Carrillo, L., López Villezca, D., Hernández Herrera, C., López Prieto, J. J., Gaona Rodríguez, R., Villeda Espinosa, E., Flores Martínez, D., Velasco Barcena, J., Yong, R., Rodríguez Briones, I., Leiva Pons, J. L., Álvarez López, H., Olvera Ruiz, R., Díaz de la Vega, C., Cantú Brito, C., Chuquiure Valenzuela, E., Reyes-Sanchez, R., Bazzoni Ruiz, A., Nandayapa Flores, O., Benavides Gonzalez, M., Arriaga Nava, R., Morales Cerda, J. D., Fierro Fierro, O., Fajardo Campos, P., Alfaro, T. A. A., Altamirano Bellorin, S., Avena, R., Chavarria, M., Espinosa, I., Flores Silva, F., Garcia Nava, R. H., Godoy, K., Gonzalez Felix, E. J., Gonzalez Garcia, C. L., Gonzalez Salas, L. G., Guajardo, P., Hernandez Gonzalez, S., Izquierdo, T., Mancilla Ortiz, M. C., Martinez Vasquez, D., Mendoza, N., Morales, J., Nikitina, N., Ochoa Aybar, S., Ortiz, A., Padilla Macias, P., Perez, F., Perez Sanchez, J. A., Piña Toledano, S., Ramos Gonzalez, M., Rivera Ramos, C., Roa Castro, V., Romero Cardona, G., Ruiz Cornejo, M., Salinas, A., Santana, G., Sida Perez, P., Tovar Castaneda, A. C., Trujillo Cortes, R., Brodmann, M., Lenz, K., Drexel, H., Foechterle, J., Hagn, C., Podczeck-Schweighofer, A., Huber, K., Winkler, M., Schneeweiß, B., Gegenhuber, A., Lang, W., Eichinger-Hasenauer, S., Kaserer, P., Sykora, J., Rasch, H., Pichler, M., Schaflinger, E., Strohmer, B., Breier, R., Ebner, K. -M., Eischer, L., Freihoff, F., Lischka-Lindner, A., Mark, T., Mirtl, A., Said, A., Stöcklöcker, C., Vogel, B., Vonbank, A., Wöhrer, C., Zanolin, D., Cools, F., Paparella, G., Vandergoten, P., Parqué, J. -L., Capiau, L., Vervoort, G., Wollaert, B., Desfontaines, P., Mairesse, G., Boussy, T., Godart, P., De Wolf, A., Voet, J., Heyse, A., Hollanders, G., Anné, W., Vercammen, J., Purnode, P., Blankoff, I., Faes, D., Balthazar, Y., Beutels, M., Maréchal, P., Verstraete, S., Xhaet, O., Striekwold, H., Thoeng, J., Hermans, K., Alzand, B., Ascoop, A. -K., Banaeian, F., Barbuto, A. -M., Billiaux, A. C., Blockmans, M., Bouvy, C., Brike, C., Capiau, H., Casier, T., Conde Y Bolado, A., De Cleen, D., De Coninck, M., de Vos, M., de Weerdt, N., Delforge, M., Delvigne, M., Denie, D., Derycker, K., Deweerd, E., Dormal, F., Drieghe, S., Everaert, M., Eykerman, T., Feys, E., Ghekiere, M., Gits, F., Hellemans, S., Helvasto, L., Jacobs, C., Lips, S., Mestdagh, I., Nimmegeers, J., Piamonte, V., Pollet, P., Postolache, A., Raepers, M., Raymenants, E., Richa, J., Rombouts, H., Salembier, J., Scheurwegs, C., Semeraro, O., Simons, N., Smessaert, C., Smolders, W., Stockman, I., Tahon, S., Thyssen, V., Tincani, G., Van Durme, F., Van Lier, D., Vandekerckhove, H., Vandekerckhove, Y., Vandenbroeck, D., Vandorpe, A., Vanhalst, E., Vanhauwaert, B., Vantomme, C., Vergauwen, L., Verloove, H., Vydt, T., Weyn, T., Jansky, P., Reichert, P., Spacek, R., Machova, V., Zidkova, E., Ludka, O., Olsr, J., Kotik, L., Plocova, K., Racz, B., Ferkl, R., Hubac, J., Kotik, I., Monhart, Z., Burianova, H., Jerabek, O., Pisova, J., Petrova, I., Dedek, V., Honkova, M., Podrazil, P., Spinar, J., Vitovec, J., Novak, M., Lastuvka, J., Durdil, V., Antonova, P., Bockova, L., Bultas, J., Chlumsky, J., Dastychova, L., Drasnar, T., Honek, J., Horejsi, M., Hubacova, V., Janska, L., Kopeckova, I., Kratochvilova, R., Krcova, E., Labrova, R., Lindourkova, A., Lipoldova, J., Lubanda, H., Ludkova, A., Mahdalikova, L., Majerníkova, M., Michalik, D., Potuznik, P., Prochazkova, E., Sulc, A., Sveceny, J., Valtova, M., Zidek, M., Zika, J., Nielsen, J., Nielsen, H., Husted, S., Hintze, U., Rasmussen, S., Bremmelgaard, A., Markenvard, J., Boerger, J., Solgaard, J., Simonsen, P., Loekkegaard, T., Bruun, M., Mertz, J., Domínguez, H., Skagen, K., Egstrup, K., Ibsen, H., Raymond, I., Bang-Hansen, T., Ellervik, C., Eriksen, E., Jensen, L., Jensen, M., Leth, M., Nygaard, A., Park, J., Schou, M., Therkelsen, A., Tilma, J., Vesterager, K., Raatikainen, P., Airaksinen, J., Arola, O., Koistinen, J., Nappila, H., Peltomäki, K., Rasanen, V., Vasankari, T., Le Heuzey, J. -Y., Galinier, M., Gottwalles, Y., Paganelli, F., Loiselet, P., Muller, J. -J., Koujan, M. B., Marquand, A., Destrac, S., Piot, O., Delarche, N., Cebron, J. -P., Boveda, S., Guenoun, M., Guedj-Meynier, D., Galley, D., Ohayon, J., Assouline, S., Zuber, M., Amarenco, P., Ellie, E., Kadouch, J., Fournier, P. -Y., Huberman, J. -P., Lemaire, M., Rodier, G., Milandre, L., Vandamme, X., Sibon, I., Neau, J. -P., Mahagne, M. H., Mielot, A., Bonnefoy, M., Churet, J. -B., Navarre, V., Sellem, F., Monniot, G., Boyes, J. -P., Doucet, B., Martelet, M., Obadia, D., Crousillat, B., Mouallem, J., Bearez, E., Nazeyrollas, P., Brugnaux, J. P., Fedorowsky, A., Casassus, F., Berneau, J. -B., Chemin, F., Falvo, N., Perron, J. -M., Poulard, J. -E., Barreau, A., Beltra, C., Corrihons, E., Decarsin, N., Dubois, B., Ducasse, E., Giry, X., Kemmel, A., Ledure, S., Lemaire, N., Robin, F., Rosolin, N., Sanchez, D., Suissa, A., Darius, H., Königer, G., Purr, J., Gerbaulet, U., Kellner, B. -T., Kopf, A., Schäfer, T., Zauzig, H., Riegel, P., Hohensee, H., Eißfeller, E., Eder, W., Rehling, G., Glatzel, D., Zutz, S., Heinz, G. -U., Menke, H., Pustelnik, A., Sandow, P., Ludwig, N., Wiswedel, H., Wildenauer, W., Axthelm, C., Schwarz, T., Babyesiza, A., Stuchlik, G., Zimny, H. -H., Kropp, M., Kahl, F., Caspar, A., Omankowsky, S., Läßig, T., Hartmann, H. -J., Lehmann, G., Bindig, H. -W., Hergdt, G., Reimer, D., Hauk, J., Dorsch, W., Dshabrailov, J., Michel, H., Rapp, K. -A., Vormann, R., Mayer, P., Horstmeier, U., Eissing, V., Hey, H., Leuchtgens, H., Lilienweiß, V., Kolitsch, K., Schubert, C., Lauer, H., Buchner, T., Brauer, G., Kamin, S., Müller, K., Abdel-Qader, M., Baumbach, S., Ebert, H. -H., Schwencke, C., Schellong, S., Bernhardt, P., Karolyi, L., Sievers, B., Haverkamp, W., Salbach, P., Röhnisch, J. -U., Schoen, S., Erdle, W., Mueller, T., Mueller, H., Mitrovic, V., Babjakova, Z., Bergner, K., Boehme, S., Bonin, K., Buckert, D., Busch, F., Dichristin, U., Diez, S., Fleck, A., Flint, K., Floegel, H., Fritz, C., Frommhold, R., Gehre, J., Geyer, J., Grytzmann, A., Hahn, M., Helgert, K., Hubert, K., Kirchner-Volker, K., Klein, V., Kroll, D., Krueger, A., Lehmann, R., Mann, L., Maselli, A., Menken, G., Mikes, K., Mortan, H., Nasser, N., Nicolaus, D., Plauskat, A., Pomper, L., Quietzsch, A., Ravenhorst, C., Reichelt, C., Reimer, C., Schaefer, B., Scharrer, S., Schirmer, K., Schmidt, K., Schoene, R., Schulze, J., Schuppe, M., Simon, S., Sommer, S., Spranger, K., Talkenberger, A., Tauber, K., Tetlak, A., Toennishoff, T., Voelkel-Babyesiza, R., Voigts, B., Weiser, U., Wesendorf, S., Wildenauer, S., Wolf, T., Wurziger, J., Zak, J., Zauzig, H. -D., Ziefle, S., Zincke, S., Keltai, M., Vangel, S., Szalai, G., Merkely, B., Kancz, S., Boda, Z., Nagy, A., Laszlo, Z., Matoltsy, A., Gaszner, B., Polgar, P., Habon, T., Noori, E., Juhasz, G., Kanakaridisz, N., Szentpeteri, I., Juhasz, F., Vertes, A., Papp, A., May, Z., Ferenczi, J., Egyutt, M., Kis, E., Engelthaler, G., Szantai, G., Fulop, E., Gombos, P., Gulyas, D., Jen, P., Kiralyhazine Gyorke, E., Kovacs, M., Kovacsne Levang, S., Marianna, S., Radics, Z., Sydó, N., Szalo, R., Szilagyi, A., Sztanyik, F., Vandrus, B., Agnelli, G., Ambrosio, G., Tiraferri, E., Santoro, R., Testa, S., Di Minno, G., Moia, M., Caimi, T. M., Martini, G., Tessitori, M., Cappelli, R., Poli, D., Quintavalla, R., Melone, F., Cosmi, F., Pizzini, A., Piseddu, G., Fanelli, R., Latella, C., Santi, R., Pancaldi, L., De Cristofaro, R., Palareti, G., De Blasio, A., Salerno Uriarte, J., Minetti, F., Pogliani, E. M., Lonati, L. M., Accogli, M., Ciampani, N., Malengo, S., Feola, M., Raisaro, A., Fattore, L., Grilli, P., Germini, F., Settimi, M., Alunni, M., Duranti, G., Tedeschi, L., Baglioni, G., Avanzino, G., Berardi, M., Pannacci, V., Giombolini, A., Nicoli, S., Scarponi, T., Allasia, B., Ricciarini, P., Nasorri, R., Argena, A., Bossolasco, P., Ronchini, P., Filippi, A., Tradati, F., Bulla, C., Donzelli, L., Foppa, L., Bottarelli, M. L., Tomasello, A., Mauric, A., Femiano, C., Reggio, R., Lillo, F., Mariani, A., Forcignanò, F., Volpe, M., D'Avino, M., Bongiorni, M. G., Severi, S., Capucci, A., Lodigiani, C., Salomone, E., Serviddio, G., Tondo, C., Golino, P., Mazzone, C., Iacopino, S., Pengo, V., Galvani, M., Moretti, L., Ambrosino, P., Banfi, E., Biagioli, V., Bianchi, A., Boggian, G., Breschi, M., Brusorio, S., Calcagnoli, F., Campagna, G., Carpenedo, M., Ciabatta, C., Ciliberti, G., Cimmino, G., D'Arienzo, C., Di Gennaro, L., Fedele, M., Ferrini, P. M., Granzow, K., Guazzaloca, G., Guerra, F., Lo Buglio, A., Longo, S., Macellari, F., Mesolella, E., Mollica Poeta, E., Occhilupo, P., Oriana, V., Rangel, G., Salomone, L., Scaccianoce, A., Scarone, C., Segreti, L., Sottilota, G., Villani, R., Zecca, C., ten Cate, H., Ruiter, J. H., Klomps, H., Bongaerts, M., Pieterse, M. G. C., Guldener, C., Herrman, J. -P., Lochorn, G., Lucassen, A., Adriaansen, H., The, S. H. K., Nierop, P. R., Hoogslag, P. A. M., Hermans, W., Groenemeijer, B. E., Terpstra, W., Buiks, C., Boersma, L. V. A., Boersma-Slootweg, M., Bosman, F., Bosschaert, M., Bruin, S., Danse, I., De Graaf, J., de Graauw, J., Debordes, M., Dols, S., Geerlings, F., Gorrebeeck, K., Jerzewski, A., Jetten, W., Kelderman, M., Kloosterman, T., Koomen, E. M., Krikken, J., Melman, P., Mulder, R., Pronk, A., Stallinga-de Vos, A., te Kaat, J., Tonino, P., Uppelschoten, B., van de Loo, R., van der Kley, T., van Leeuwen, G., van Putten, J. J., Westerman, L., Atar, D., Berge, E., Sirnes, P. A., Gjertsen, E., Hole, T., Erga, K., Hallaråker, A., Skjelvan, G., Østrem, A., Ghezai, B., Svilaas, A., Christersson, P., Øien, T., Høegh Henrichsen, S., Berg-Johansen, J., Otterstad, J. E., Antonsen, H., Ausen, K., Claussen, H., Dominguez, I., Jekthammer, A., Lensebraaten, A. B., Nilsen, V., O'Donovan, M., Ringdalen, K., Strand, S., Stepinska, J., Korzeniak, R., Gieroba, A., Biedrzycka, M., Ogorek, Marcin, Wozakowska-Kaplon, B., Loboz-Grudzien, K., Kosior, J., Supinski, W., Kuzniar, J., Zaluska, R., Hiczkiewicz, J., Swiatkowska-Byczynska, L., Kucharski, L., Gruchala, M., Minc, P., Olszewski, M., Kania, G., Krzciuk, M., Lajkowski, Z., Ostrowska-Pomian, B., Lewczuk, J., Zinka, E., Karczmarczyk, A., Chmielnicka-Pruszczynska, M., Trusz-Gluza, M., Opolski, G., Bronisz, M., Ogorek, Michal, Glanowska, G., Ruszkowski, P., Jaworska, K., Sciborski, R., Okopien, B., Kukla, P., Wozniak-Skowerska, I., Galbas, K., Cymerman, K., Jurowiecki, J., Miekus, P., Myszka, W., Mazur, S., Lysek, R., Baszak, J., Rusicka-Piekarz, T., Raczak, G., Domanska, E., Nessler, J., Lesnik, J., Ambicka, M., Andrzejewski, D., Araminowicz, J., Barszcz, A., Bartkowiak, R., Bartnik, J., Basiak, M., Bekieszczuk, E., Bernat, L., Biedrzycki, L., Biernacka, A., Blaszczyk, D., Broton, E., Brzozowski, W., Brzustowska, M., Bzymek, R., Chmielowski, A., Chojnowski, P., Cichomski, R., Cieslak, K., Cieszynska, A., Curyllo, B., Czamara, M., Danilowicz-Szymanowicz, L., Dolecka, B., Drelich, L., Dudzik-Richter, B., Dybala, T., Dziuba, M., Faron, W., Figura-Chmielewska, M., Frankiewicz, A., Gadzinski, W., Gasior, E., Gosciniecka, B., Gutknecht, P., Guziewicz, M., Jackun-Podlesna, A., Jaguszewska, G., Jankielewicz, J., Jaremczuk-Kaczmarczyk, A., Jargiello-Baszak, M., Jarzebowski, A., Jaskulska-Niedziela, E., Jaworska-Drozdowska, M., Kabat, J., Kaczmarzyk-Radka, A., Kalin, K., Kaliszczak, R., Kiliszek, M., Klata, M., Kluczewski, M., Kobielusz-Gembala, I., Kochanska, E., Kociolek, D., Kolodzinska, A., Komlo, A., Konopka, A., Korczowska, E., Kowal, E., Kowalczyk, H. K., Kremis, E., Kruczyk, D., Krzesiak-Lodyga, A., Krzyzanowski, M., Kurdzielewicz, W., Kustrzycka-Kratochwil, D., Lesniewska-Krynska, D., Leszczynski, J., Lewicka, E., Lichota, E., Lip, K., Loboz-Rudnicka, M., Luka, J., Lysek-Jozefowicz, A., Machnikowska, M., Majewska, K., Mariankowski, R., Markiewicz, A., Mazur, M., Metzgier-Gumiela, A., Miedlar, E., Mielcarek, M., Neubauer-Geryk, J., Niedek, J., Niemirycz-Makurat, A., Nowak, A., Nowak, S., Opielowska-Nowak, B., Ozgowicz, M., Pawelska-Buczen, A., Pawlik-Rak, E., Piotrowicz, R., Ptaszynski, P., Raczynska, A., Rogowski, W., Romanek, J., Romaszkiewicz, R., Rostoff, P., Roszczyk, N., Rozewska-Furmanek, D., Rychta, J., Rzyczkowska, B., Sidor, A., Skalska, J., Smichura, M., Splawski, M., Staneta, P., Staniszewska, E., Starak-Marciniak, J., Stopyra-Poczatek, M., Sukiennik-Kujawa, M., Szafranski, J., Szalecki, P., Szczepanska, A., Szkrobka, W., Szuchnik, E., Szulowska, A., Szumczyk-Muszytowska, G., Szwoch, M., Traczyk, T., Troszczynska, M., Trzcinski, G., Tybura, S., Walasik, P., Wegrzynowska, M., Wesolowska, K., Wieczorek, W., Wierzbicka, A., Wilczewski, P., Wilgat-Szecowka, M., Wojewoda, P., Wojnowski, L., Wrobel, M., Zakutynska-Kowalczyk, K., Zyczynska-Szmon, M., Panchenko, E., Eltishcheva, V., Libis, R., Tereshchenko, S., Popov, S., Kamalov, G., Belenky, D., Zateyshchikova, A., Kropacheva, E., Kolesnikova, A., Nikolaev, K., Egorova, L., Khokhlov, A., Yakupov, E., Poltavskaya, M., Zateyshchikov, D., Drapkina, O., Vishnevsky, A., Barbarash, O., Miller, O., Aleksandrova, E., Chizhov, P., Sergeev, M., Shutemova, E., Mazur, E., Zrazhevskiy, K., Novikova, T., Kostenko, V., Moiseeva, Y., Polkanova, E., Sobolev, K., Rossovskaya, M., Zubeeva, G., Shapovalova, Y., Nagibovich, O., Edin, A., Agakhanyan, A., Batalov, R., Belenkova, Y., Bitakova, F., Chugunnaya, S., Dumikyan, A., Erofeeva, S., Gorbunova, E., Gorshkova, T., Gubanov, A., Gurmach, M., Ivanova, Y., Kolesova, T., Konyushenko, D., Korneeva, O., Kropova, O., Kuchuk, P., Kungurtseva, O., Kupriyanova, T., Kurylo, B., Kuvanova, M., Lebedeva, O., Lileeva, E., Machilskaya, O., Medvedeva, T., Monako, G., Motylev, I., Nagibovich, G., Novikova, E., Orlov, Y., Osmolovskaya, Y., Ovsannikova, A., Platonov, D., Rachkova, S., Sinitsina, O., Speshilova, S., Suslova, O., Ushakov, A., Volodicheva, O., Zemlianskaia, O., Zhirov, I., Zhuravleva, E., Zotova, I., Viñolas, X., Alvarez Garcia, P., López Fernández, M. F., Tercedor, L., Tranche Iparraguirre, S., Torán Monserrat, P., Márquez Contreras, E., Isart Rafecas, J., Motero Carrasco, J., García Pavía, P., Gómez Pajuelo, C., Moro Serrano, C., Iglesias Alonso, L. F., Grande Ruiz, A., Mercé Klein, J., Gonzalez Juanatey, J. R., Barón Esquivias, G., Monte Collado, I., Palacín Piquero, H., Brotons Cuixart, C., Rodríguez Morató, M., Bayo I Llibre, J., Corros Vicente, C., Vida Gutierrez, M., Epelde Gonzalo, F., Almeida Fernández, C. A., Del Val Plana, N., Escrivá Montserrat, E., Montero Alía, J. J., Barreda González, M., Moleiro Oliva, M. A., Iglesias Sanmartín, J., Jiménez González, M., Rodriguez Álvarez, M., Herreros Melenchon, J., Ripoll Vera, T., Ridocci Soriano, F., Garcia Riesco, L., Marco Macian, M. D., Quiles Granado, J., Jimenez Navarro, M., Cosin Sales, J., Vaquer Perez, J. V., Vazquez Caamano, M., Arcocha Torres, M. F., Marcos Gomez, G., Iñiguez Romo, A., Prieto Diaz, M. A., Alonso, Carmela, Alonso, Concepcion, Alvarez, D., Alvarez, M., Amaro, M., Andere, N., Aracil Villar, J., Armitano Ochoa, R., Austria, A., Barbeira, S., Barraquer Feu, E., Bartes, A., Becerra Munoz, V., Bermudez Jimenez, F. J., Branjovich Tijuan, A., Cabeza Ramirez, J., Cabrera Ramos, M., Calvo Martinez, E., Campo Moreno, M., Cancho Corchado, G., Casanova Gil, M., Castillo Orive, M., Castro Fernandez, D., Cebollada del Misterio, M., Codinachs Alsina, R., Cortada Cabrera, A., Costa Pinto Prego de Faria, J., Costas, S., Cotilla Marco, M. I., Dachs, M., Diaz Lopez, C. M., Domenech Borras, A., Elorriaga Madariaga, A., Espallargas, A., Fernandez, M., Fernandez Escobar, E., Fernandez Mas, E., Ferrer, A., Fosch, J., Garcia Bermudez, M., Garcia Millan, V., Gavira Saenz, M., Gines Garcia, C., Gomez, C., Gomez Perez, Y., Gonzales Segovia, A., Gonzalez, P., Grigorian, L., Guerrero Molina, A., del C. Gutierrez del Val, M., Herrero Maeso, B., Hevia Rodriguez, E., Iglesias Garcia, A., Jimenez Fernandez, M. J., Jimeno Besa, B., Juan Salvadores, P., Lage Bouzamayor, M. B., Lasuncion, I., Lezcano Gort, L. E., Llobet Molina, M., Lopez, M., Manzanal Rey, A., Mara Guerra, J., Marcus, S., Martin Vila, A., Martinez Mena, M., Mazon, P., Mendez Zurita, F., Millán, G., Molina, M., Montero Alia, P., Montes, D., Moure Gonzalez, M., Munoz Munoz, R. B., Negrete Palma, A., Orellana Figueroa, H. N., Ortega, V. M., Ortiz Cortes, C., Otero Tomera, D., Palomo Merchan, N., Pareja Ibar, I., Pena Garcia, E., Pereda Armayor, M., Perez Carasa, M., Prieto, I., Quintern, V., Renom, R., Rincon Diaz, L. M., Rios, V., Riquelme Sola, L., Rivera, R., Robiro Robiro, X., Roca, M., Roca Saumell, C., Rodrigo, C., Rodriguez, E., Rodriguez Garcia, M., Saez Jimenez, S., Sanchez Calderon, P., Sanchez Mendez, L., Sanchez Parra, S., Santolaya, C., Senan Sanz, M. R., Seoane Blanco, A., Serralvo, E., Sierra, N., Simon Valero, C., Sorribes Lopez, J., Teixido Fontanillas, M., Terns Riera, M., Tobajas, G., Torres, C., Torres Marques, J., Ubeda Pastor, M., Rosenqvist, M., Wirdby, A., Linden, J., Henriksson, K., Elmersson, M., Egilsson, A., Börjesson, U., Svärd, G., Liu, B., Lindh, A., Olsson, L. -B., Gustavsson, M., Andersson, Lar, Andersson, Lisbeth, Benson, L., Bothin, C., Hajimirsadeghi, A., Kadir, K., Ericsson, M., Ohlsson, A., Lindvall, H., Svensson, P., Thorne, K., Handel, H., Platonov, P., Eriksson, B., Timberg, I., Romberg, K., Crisby, M., Karlsson, J. -E., Jensen, S. A., Andersson, A., Malmqvist, L., Martinsson, B., Bernsten, F., Engdahl, J., Thulin, J., Hot-Bjelac, A., Stalby, P., Aaröe, H., Ahbeck, E., Ahlmark, H., Al-Khalili, F., Bonkowski, G., Dzeletovic, S., Ekstrand, A. -B., Eriksson, G. -B., Floren, K., Grässjö, C., Hahn, S., Jaensson, P., Jansson, B., Jansson, J. -H., Kangert, R. -M., Koch, A., Kusiak, D., Lettenström, A., Lindberg, A., Lindholm, C. -J., Mannermyr, A., Mansson, K., Millborg, M., Nilsson, C., Ohlin, A. -M., Olofsson, A., Osberg, A., Pedersen, A., Risbecker, K., Rosenberg, K., Samuelsson, J., Shayesteh, M., Skoglund, K., Stjernberg, M., Thorsen, C., Steffel, J., Beer, J. H., Debrunner, J., Amstutz, D., Bruegger, J., Elise, G., Grau, A., Guinand, A., Henriette, I., Saga, E., Winnik, S., Parkhomenko, A., Rudyk, I., Tseluyko, V., Karpenko, O., Zhurba, S., Kraiz, I., Kupnovytska, I., Serediuk, N., Mostovoy, Y., Ushakov, O., Koval, O., Kovalskyi, I., Svyshchenko, Y., Sychov, O., Stanislavchuk, M., Kraydashenko, O., Yagensky, A., Tykhonova, S., Fushtey, I., Belegai, R., Berko, G., Burdeuna, L., Chabanna, O., Daniuk, I., Ivanov, A., Kamenska, E., Kaplan, P., Khyzhnyak, O., Kizim, S., Matova, O., Medentseva, O., Mochonyi, V., Mospan, M., Nemtsova, V., Ovdiienko, T., Palamarchuk, O., Pavelko, M., Petrovskyy, R., Plevak, D., Proshak, O., Pyvovar, S., Rasputina, L., Romanenko, O., Romanova, O., Sapatyi, A., Shumakov, O., Stets, R., Todoriuk, L., Varenov, V., Fitzmaurice, D., Chauhan, N., Goodwin, D., Saunders, P., Evans, R., Leese, J., Jhittay, P. S., Ross, A., Kainth, M. S., Pickavance, G., Mcdonnell, J., Williams, A., Gooding, T., Wagner, H., Suryani, S., Singal, A., Sircar, S., Bilas, R., Hutchinson, P., Wakeman, A., Stokes, M., Paul, N., Aziz, M., Ramesh, C., Wilson, P., Franklin, S., Fairhead, S., Thompson, J., St Joseph, V., Taylor, G., Tragen, D., Seamark, D., Paul, C., Richardson, M., Jefferies, A., Sharp, H., Jones, H., Giles, C., Page, M., Oginni, O., Aldegather, J., Wetherwell, S., Lumb, W., Evans, P., Scouller, F., Macey, N., Stipp, Y., West, R., Thurston, S., Wadeson, P., Matthews, J., Pandya, P., Gallagher, A., Railton, T., Sinha, B., Russell, D., Davies, J. A., Ainsworth, P., Jones, C. P., Weeks, P., Eden, J., Kernick, D., Murdoch, W., Lumley, L., Patel, R. P., Wong, S. W., Saigol, M., Ladha, K., Douglas, K., Cumberlidge, D. F., Bradshaw, C., Van Zon, G., Jones, K. P., Thomas, M. J., Watson, E., Sarai, B., Ahmad, N., Willcock, W., Cairns, J., Sathananthan, S., de Kare-Silver, N., Gilliland, A., Strieder, E., Howitt, A., Vishwanathan, B., Bird, N., Gray, D., Clark, M., Bisatt, J., Litchfield, J., Fisher, E., Fooks, T., Kelsall, A. R., Alborough, E., Wakeling, J., Parfitt, M., Milne, K., Rogers, S., Priyadharshan, R., Oliver, J. L., Davies, E., Abushal, S., Jacobs, M., Hutton, C., Walls, N. I., Thompson, R., Chigbo, C., Zaidi, S. M. A., Howard, M., Butter, K. C., Barrow, S., Little, H., Haq, I. U., Gibbons, L., Glencross, S., Mcleod, A. J., Poland, K., Mulholland, C., Warke, A., Conn, P., Burns, G., Smith, R. N., Lowe, S., Kamath, R., Dau, H. S., Webster, J., Hodgins, I., Vercoe, S., Roome, P. C., Pinnock, H., Patel, J. R. A., Ali, A., Hart, N., Davies, R., Stuart, E., Neden, C. A., Danielsen, M., Heath, R., Sharma, P., Galloway, S., Hawkins, C., Oliver, R., Aylward, M., Mannion, S., Braddick, M., Edwards, D., Rothwell, A. C., Sabir, A., Choudhary, F., Khalaque, S., Wilson, A., Peters, S., Coulson, W., Roberts, N., Heer, A., Coates, S., Ward, B., Jackson, D., Walton, S., Shepherd, D., Sterry, M., Wong, T., Boon, M., Bunney, R., Haria-Shah, R., Baron, R. T., Davies, S., Schatzberger, T., Hargreaves, N., Stephenson, T., Choi, H., Batson, R., Lucraft, L., Myhill, T., Estifano, S., Geatch, D., Wilkinson, J., Veale, R., Forshaw, K., Davies, T., Zaman, K., Vinson, P., Liley, C., Bandrapalli, M., Mcginty, P., Wastling, R., Mceleny, P., Beattie, A., Cooke, P., Wong, M., Gunasegaram, J., Pugsley, M., Ahmad, S., A'Court, C., Ayers, J., Bennett, J., Cartwright, S., Dobson, S., Dooldeniya, C., Flynn, A., Fox, R., Goram, J., Halpin, A., Hay, A., Jacobs, P., Jeffers, L., Lomax, L., Munro, I., Muvva, R., Nadaph, M., Powell, K., Randfield, S., Redpath, D., Reed, R., Rickenbach, M., Rogers, G., Saunders, P. B., Seamark, C., Shewring, J., Simmons, P., Simper, H., Stoddart, H., Sword, A., Thomas, N., Thomson, A., Gibbs, H., Blenkhorn, A., Singh, B., Van Gaal, W., Abhayaratna, W., Lehman, R., Roberts-Thomson, P., Kilian, J., Coulshed, D., Catanchin, A., Colquhoun, D., Kiat, H., Eccleston, D., French, J., Zimmett, L., Ayres, B., Phan, T., Blombery, P., Crimmins, D., O'Donnell, D., Choi, A., Astridge, P., Arstall, M., Jepson, N., Binnekamp, M., Lee, A., Rogers, J., Starmer, G., Carroll, P., Faunt, J., Aggarwala, A., Barry, L., Batta, C., Beveridge, R., Black, A., Bonner, M., Boys, J., Buckley, E., Campo, M., Carlton, L., Connelly, A., Conway, B., Cresp, D., Dimitri, H., Dixon, S., Dolman, M., Duroux, M., Eskandari, M., Eslick, R., Ferreira-Jardim, A., Fetahovic, T., Fitzpatrick, D., Geraghty, R., Gibbs, J., Grabek, T., Modi, M. H., Hayes, K., Hegde, M. P., Hesketh, L., Hoffmann, B., Jacobson, B., Johnson, K., Juergens, C., Kassam, I., Lawlor, V., Lehman, M., Lehman, S., Leung, D., Mackay, S., Mackenzie, M., Mccarthy, C., Mcintosh, C., Mckeon, L., Morrison, H., Mussap, C., Myers, J. -D., Nagalingam, V., Oldfield, G., O'May, V., Palmer, J., Parsons, L., Patching, K., Patching, T., Paul, V., Plotz, M., Preston, S., Rashad, H., Ratcliffe, M., Raynes, S., Rose, J., Sanders, L., Seremetkoska, M., Setio, H., Shone, S., Shrestha, P., Singh, C., Singleton, C., Stoyanov, N., Sutcliffe, S., Swaraj, K., Tarrant, J., Thompson, S., Tsay, I. M., Vorster, M., Waldman, A., Wallis, L., Wilford, E., Wong, K., Connolly, S. J., Spyropoulos, A., Eikelboom, J., Luton, R., Gupta, M., Pandey, A. S., Cheung, S., Leader, R., Beaudry, P., Ayala-Paredes, F., Berlingieri, J., Heath, J., Poirier, G., Du Preez, M., Nadeau, R., Dresser, G., Dhillon, R., Hruczkowski, T., Schweitzer, B., Coutu, B., Angaran, P., Macdonald, P., Vizel, S., Fikry, S., Parkash, R., Lavoie, A., Cha, J., Ramjattan, B., Bonet, J., Ahmad, K., Aro, L., Aves, T., Beaudry, K., Bergeron, C., Bigcanoe, J., Bignell, N., Breakwell, L., Burke, E., Carroll, L., Clarke, B., Cleveland, T., Daheb, S., Dehghani, P., Denis, I., Djaidani, Z., Dorian, P., Douglass, S., Dunnigan, J., Ewert, A., Farquhar, D., Fearon, A., Ferleyko, L., Fournier, D., Fox, B., Grenier, M. -C., Gulliver, W., Haveman, K., Hines, C., Hines, K., Jackson, A. M., Jean, C., Jethoo, G., Kahlon, R., Kelly, S., Kim, R., Korley, V., Kornder, J., Kwan, L., Largy, J., Lewis, C., Lewis, S., Mangat, I., Moor, R., Navratil, J., Neas, I., Otis, J., Otis, R., Pandey, M., Petrie, F., Pinter, A., Raines, M., Roberts, P., Robinson, M., Sas, G., Schulman, S., Snell, L., Spearson, S., Stevenson, J., Trahey, T., Wong, S., Wright, D., Ragy, H., Abd El-Aziz, A., Abou Seif, S. K., El Din, M. G., El Etriby, S., Elbahry, A., El-Etreby, A., Elkhadem, M., Katta, A., Khairy, T., Mowafy, A., Nawar, M., Ohanissian, A., Reda, A., Reda, M., Salem, H., Sami, N., Samir, S., Setiha, M., Sobhy, M., Soliman, A., Taha, N., Tawfik, M., Zaatout, E., Kettles, D., Bayat, J., Siebert, H., Horak, A., Kelfkens, Y., Garda, R., Pillay, T., Guerra, M., van Zyl, L., Theron, H., Murray, A., Louw, R., Greyling, D., Mntla, P., Ueckermann, V., Loghdey, R., Ismail, S., Ahmed, F., Engelbrecht, J., Ramdass, A., Maharajh, S., Oosthuysen, W., Angel, G., Bester, C., Booysen, M., Boshoff, C., Cannon, C., Cassimjee, S., Chami, C., Conway, G., Davids, A., de Meyer, L., Du Plessis, G., Ellis, T., Henley, L., Karsten, M., Loyd, E., Marks, J., Mavhusa, L., Mostert, M., Page, A., Rikhotso, L., Salie, M., Sasto, J., Shaik, F., Skein, A., Smith, L., Tarr, G., Tau, T., van Zyl, F., Al Mahmeed, W., Yousef, G., Agrawal, A., Nathani, M., Ibrahim, M., Esheiba, E. M., Singh, R., Naguib, A., Abu-Mahfouz, M., Al Omairi, M., Al Naeemi, A., Maruthanayagam, R., Bazargani, N., Wassef, A., Gupta, R., Khan, M., Subbaraman, B., Abdul, A., Al Mulla, A., El Bardisy, S., Haridas, P., Jadhav, S., Magdaluyo, K., Makdad, M., Maqsood, I., Mohamed, R., Sharma, N., Sharma, R., Thanzeel, M., Goldhaber, S. Z., Canosa, R., Rama, P., Blumberg, E., Garcia, J., Mullen, P., Wilson, V., Quick, A., Ferrick, K., Kutayli, W. M., Cox, M., Franco, M., Falkowski, S., Mendelson, R., Williams, M., Miller, S., Beach, S., Alfieri, A., Gutowski, T., Haque, I., Reddy, R., Ahmed, W., Delafontaine, P., Diercks, D., Theodoro, D., Remmel, K., Alberts, M., Ison, R., Noveck, H., Duffy, P., Pitta, S., Nishijima, D., Treasure, C., Asafu-Adjaye, N., Ball, K., Bartlett, M., Bentley, M., Bowers, S., Brown, A., Browne, A., Cameron-Watts, J., Canova, M., Cassidy, D., Cervellione, K., Congal, S., Depauw, J., Dickerson, A., Eley, M., Evans, L., Felpel, S., Ferdinand, K., Fielder, D., Gentry, P., Haideri, A., Hakimi, F., Harbour, T., Hartranft, E., Hawkins, B., Headlee, M., Henson, L., Herrick, C., Hicks, T., Jasinski, S., Jones, A., Jones, L., Jones, P., Karl, S., Keeling, M., Kerr, J., Knowles, P., Langdon, J., Lay, M., Lee, J. A., Lincoln, T., Malone, E., Merliss, A., Merritt, D., Minardo, J., Mooso, B., Orosco, C., Palumbo, V., Parker, M., Parrott, T., Paserchia, S., Pearl, G., Peterson, J., Pickelsimer, N., Purcell, T., Raynor, J., Raziano, S., Richard, C., Richardson, T., Robertson, C., Sage, A., Sanghera, T., Shaw, P., Shoemaker, J., Smith, K., Stephanie, B., Thatcher, A., Theobald, H., Thompson, N., Treasure, L., Tripti, T., Verdi, C., and Worthy, V.
Subjects: Registrie, Male, Time Factors, Anticoagulant therapy, Arrhythmia, Atrial fibrillation, GARFIELD-AF, 030204 cardiovascular system & hematology, Electrocardiography, 0302 clinical medicine, Risk Factors, Anticoagulant therapy, Arrhythmia, Atrial fibrillation, GARFIELD-AF, Aged, Atrial Fibrillation, Female, Follow-Up Studies, Humans, Incidence, India, Prevalence, Prognosis, Prospective Studies, Survival Rate, Thromboembolism, Registries, Risk Assessment, Medicine, 030212 general & internal medicine, Prospective cohort study, Incidence (epidemiology), cardiovascular system, medicine.symptom, Cardiology and Cardiovascular Medicine, Risk assessment, Human, medicine.medical_specialty, Time Factor, RD1-811, Prognosi, Follow-Up Studie, 03 medical and health sciences, Internal medicine, Diseases of the circulatory (Cardiovascular) system, cardiovascular diseases, Risk factor, Survival rate, Fibrillation, business.industry, Risk Factor, ta3121, medicine.disease, Clinical trial, Prospective Studie, RC666-701, Surgery, Clinical and Preventive Cardiology, business
Abstract: Background: The Global Anticoagulant Registry in the FIELD–Atrial Fibrillation (GARFIELD-AF) is an ongoing prospective noninterventional registry, which is providing important information on the baseline characteristics, treatment patterns, and 1-year outcomes in patients with newly diagnosed non-valvular atrial fibrillation (NVAF). This report describes data from Indian patients recruited in this registry. Methods and results: A total of 52,014 patients with newly diagnosed AF were enrolled globally; of these, 1388 patients were recruited from 26 sites within India (2012–2016). In India, the mean age was 65.8 years at diagnosis of NVAF. Hypertension was the most prevalent risk factor for AF, present in 68.5% of patients from India and in 76.3% of patients globally (P
Published: 2018
Full Text: View/download PDF

22. <scp>NF</scp> ‐κB inhibition reverses acidic bile‐induced miR‐21, miR‐155, miR‐192, miR‐34a, miR‐375 and miR‐451a deregulations in human hypopharyngeal cells

Author: Dimitra P. Vageli, Clarence T. Sasaki, and Sotirios G. Doukas
Subjects: miR‐34a, 0301 basic medicine, Transcription, Genetic, chemistry.chemical_compound, 0302 clinical medicine, miR‐155, Mir-375, Neoplasms, Sulfones, miR‐192, Phosphorylation, Luciferases, Cells, Cultured, Chemistry, NF-kappa B, BAY 11‐7082, Hypopharyngeal cancer, Phenotype, Gene Expression Regulation, Neoplastic, Protein Transport, 030220 oncology & carcinogenesis, Molecular Medicine, Original Article, Cell Survival, Bile Acids and Salts, miR-155, 03 medical and health sciences, miR‐375, Nitriles, microRNA, medicine, Humans, Gene, miR‐21, Cell Nucleus, bile acids, miR‐451a, NF‐κB, NF-κB, Original Articles, Cell Biology, medicine.disease, In vitro, Hypopharynx, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, Cancer research, hypopharyngeal cancer
Abstract: We previously demonstrated that acidic bile activates NF‐κB, deregulating the expression of oncogenic miRNA markers, in pre‐malignant murine laryngopharyngeal mucosa. Here, we hypothesize that the in vitro exposure of human hypopharyngeal cells to acidic bile deregulates cancer‐related miRNA markers that can be reversed by BAY 11‐7082, a pharmacologic NF‐κB inhibitor. We repetitively exposed normal human hypopharyngeal primary cells and human hypopharyngeal keratinocytes to bile fluid (400 μmol/L), at pH 4.0 and 7.0, with/without BAY 11‐7082 (20 μmol/L). We centred our study on the transcriptional activation of oncogenic miR‐21, miR‐155, miR‐192, miR‐34a, miR‐375, miR‐451a and NF‐κB‐related genes, previously linked to acidic bile‐induced pre‐neoplastic events. Our novel findings in vitro are consistent with our hypothesis demonstrating that BAY 11‐7082 significantly reverses the acidic bile‐induced oncogenic miRNA phenotype, in normal hypopharyngeal cells. BAY 11‐7082 strongly inhibits the acidic bile‐induced up‐regulation of miR‐192 and down‐regulation of miR‐451a and significantly decreases the miR‐21/375 ratios, previously related to poor prognosis in hypopharyngeal cancer. This is the first in vitro report that NF‐κB inhibition reverses acidic bile‐induced miR‐21, miR‐155, miR‐192, miR‐34a, miR‐375 and miR‐451a deregulations in normal human hypopharyngeal cells, suggesting that acidic bile‐induced events are directly or indirectly dependent on NF‐κB signalling.
Published: 2018
Full Text: View/download PDF

23. Prognostic significance of PD-L1 expression on circulating tumor cells in patients with head and neck squamous cell carcinoma

Author: Christos Perisanidis, Amanda Psyrri, Panagiota Economopoulou, Martine Mazel, Catherine Alix-Panabières, Evi Lianidou, Clarence T. Sasaki, Georgios Papaxoinis, Martha Zavridou, Margaritis Avgeris, Areti Strati, George Koutsodontis, Ioannis Kotsantis, Ilias Angelidis, and Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)
Subjects: Male, PD-L1, 0301 basic medicine, Oncology, medicine.medical_specialty, [SDV]Life Sciences [q-bio], medicine.medical_treatment, circulating tumor cells, head and neck squamous cell carcinoma, Polymerase Chain Reaction, B7-H1 Antigen, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Circulating tumor cell, Cancer immunotherapy, Limit of Detection, Internal medicine, Humans, Medicine, Liquid biopsy, Prospective cohort study, Aged, cancer immunotherapy, liquid biopsy, biology, Squamous Cell Carcinoma of Head and Neck, business.industry, Reproducibility of Results, Induction chemotherapy, Epithelial cell adhesion molecule, Hematology, Middle Aged, Neoplastic Cells, Circulating, Prognosis, medicine.disease, Survival Analysis, Head and neck squamous-cell carcinoma, 030104 developmental biology, chemistry, Head and Neck Neoplasms, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, biology.protein, Female, business, checkpoint inhibitors
Abstract: International audience; Background: Successful application of programmed death 1 (PD1) checkpoint inhibitors in the clinic may ultimately benefit from appropriate patient selection based upon predictive biomarkers. Molecular characterization of circulating tumor cells (CTC) is crucial for the investigation of molecular-targeted therapies while predictive biomarkers for response to PD1 checkpoint inhibitors are lacking. We sought to assess whether overexpression of PD-L1 in CTCs could be detected at baseline and at different timepoints during treatment in a prospective cohort of head and neck squamous cell carcinoma (HNSCC) patients and used to predict clinical outcome after treatment with curative intent. Patients and methods: We developed a highly sensitive, specific and robust RT-qPCR assay for PD-L1 mRNA expression in EpCAM(+) CTCs. In a prospective cohort of 113 locally advanced HNSCC patients treated with curative intent we evaluated PD-L1 expression in the EpCAM(+) CTC fraction at baseline, after 2 cycles of induction chemotherapy (week 6) and at the end of concurrent chemoradiotherapy (week 15). Results: PD-L1 overexpression was found in 24/94 (25.5%) patients at baseline, 8/34 (23.5%) after induction chemotherapy and 12/54 (22.2%) patients at the end of treatment. Patients with CTCs overexpressing PD-L1 at end of treatment had shorter progression-free survival (P = 0.001) and overall survival (P \textless 0.001). Multivariate analysis revealed that PD-L1 overexpression at end of treatment was independent prognostic factor for progression-free survival and overall survival. The absence of PD-L1 overexpression at the end of treatment was strongly associated with complete response with an odds ratio = 16.00 (95% CI = 2.76-92.72, P = 0.002). Conclusions: We demonstrate that detection of CTCs overexpressing PD-L1 is feasible and may provide important prognostic information in HNSCC. Our results suggest that adjuvant PD1 inhibitors deserve evaluation in HNSCC patients in whom PD-L1(+) CTCs are detected at the end of curative treatment.
Published: 2017
Full Text: View/download PDF

24. Mental fatigue is linked with attentional bias for sad stimuli

Author: Akihiro T. Sasaki, Kenji Mizuseki, Kyosuke Watanabe, Kei Mizuno, Kanako Tajima, and Yasuyoshi Watanabe
Subjects: Adult, Male, 0301 basic medicine, medicine.medical_specialty, Emotions, lcsh:Medicine, Audiology, Attentional bias, behavioral disciplines and activities, Article, Task (project management), Attentional Bias, 03 medical and health sciences, 0302 clinical medicine, Task Performance and Analysis, Human behaviour, medicine, Chronic fatigue syndrome, Humans, lcsh:Science, Depression (differential diagnoses), Multidisciplinary, Working memory, lcsh:R, Cognition, Mental Fatigue, medicine.disease, Affect, 030104 developmental biology, Preclinical research, Stroop Test, Anxiety, Female, lcsh:Q, medicine.symptom, Psychology, Photic Stimulation, 030217 neurology & neurosurgery, Stroop effect
Abstract: Previous studies have revealed that patients with chronic fatigue syndrome and affective disorders (such as depression and anxiety disorders) exhibit a vigilant attentional bias toward negative emotional stimuli. However, it remains unclear whether the change in an attentional bias for negative emotional stimuli can be induced by mental fatigue in healthy individuals. To address this question, we examined healthy participants’ (n = 27) performance in a visual probe task and emotional Stroop task before and after the mental-fatigue-inducing task. We demonstrated that acute mental fatigue induced by the long-lasting working memory task led to the alteration of cognitive processing of negative emotional information in the healthy volunteers.
Published: 2019
Full Text: View/download PDF

25. Role of the right anterior insular cortex in joint attention-related identification with a partner

Author: Norihiro Sadato, Shuntaro Okazaki, Koji Shimada, Saori Adachi-Abe, Akihiro T. Sasaki, Hiroki C. Tanabe, Haruka K. Takahashi, Kazufumi Yoshihara, Takahiko Koike, Eri Nakagawa, and Sho K. Sugawara
Subjects: Adult, Male, Joint attention, Cognitive Neuroscience, Eye contact, Experimental and Cognitive Psychology, Original Manuscript, Insular cortex, 050105 experimental psychology, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Selection (linguistics), Humans, 0501 psychology and cognitive sciences, Attention, Interpersonal Relations, Mirror neuron, Cerebral Cortex, 05 social sciences, General Medicine, Gaze, Object (philosophy), Magnetic Resonance Imaging, Female, Identification (psychology), Psychology, 030217 neurology & neurosurgery, Cognitive psychology
Abstract: Understanding others as intentional agents is critical in social interactions. We perceive others’ intentions through identification, a categorical judgment that others should work like oneself. The most primitive form of understanding others’ intentions is joint attention (JA). During JA, an initiator selects a shared object through gaze (initiative joint attention, IJA), and the responder follows the direction of the initiator’s gaze (reactive joint attention, RJA). Therefore, both participants share the intention of object selection. However, the neural underpinning of shared intention through JA remains unknown. In this study, we hypothesized that JA is represented by inter-individual neural synchronization of the intention-related activity. Additionally, JA requires eye contact that activates the limbic mirror system; therefore, we hypothesized that this system is involved in shared attention through JA. To test these hypotheses, participants underwent hyperscanning fMRI while performing JA tasks. We found that IJA-related activation of the right anterior insular cortex of participants was positively correlated with RJA-related activation of homologous regions in their partners. This area was activated by volitional selection of the target during IJA. Therefore, identification with others by JA is likely accomplished by the shared intentionality of target selection represented by inter-individual synchronization of the right anterior insular cortex.
Published: 2019

26. Factors associated with possession of accurate knowledge regarding occupational health management among operations leaders of radiation decontamination workers in Fukushima, Japan: a cross-sectional study

Author: Yusuke Masuishi, Takeyasu Kakamu, Shota Endo, Hideaki Kasuga, Sei Sato, Tomohiro Kumagai, T Sasaki, Tetsuhito Fukushima, and Tomoo Hidaka
Subjects: Adult, Male, Cross-sectional study, Anxiety, Occupational safety and health, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, human resource management, Japan, Occupational Exposure, Surveys and Questionnaires, Medicine, Fukushima Nuclear Accident, Humans, Workplace, Decontamination, Occupational Health, Occupational and Environmental Medicine, Medical education, Health management system, business.industry, Research, General Medicine, Possession (law), Middle Aged, Radiation Exposure, 030210 environmental & occupational health, Occupational Diseases, Leadership, Cross-Sectional Studies, Knowledge Management, Logistic Models, Human resource management, Nuclear Power Plants, business, Working environment
Abstract: ObjectivesAn operations leader (OL) takes an important role in occupational health management for radiation decontamination workers in Japan, and candidates for the position must participate in a training session to acquire the necessary knowledge as required by law. However, it has not been clarified whether the candidates for the OL position actually possess accurate knowledge regarding occupational health management for such work after the training session. We, therefore, aimed at examining the current occupational health management knowledge among the candidates and investigating factors related to the knowledge, with hypothesis that possession of accurate knowledge is associated with prior experience of having worked in radiation decontamination.DesignA cross-sectional study.SettingThe training sessions held by Fukushima Prefecture Labor Standard Associations in Fukushima, Japan, in 2017.ParticipantsEighty male candidates participated in the training sessions.OutcomeThe number/proportion of correct answers to the questions regarding occupational health management, such as those on working environment management, control of operations and health management.ResultsThe proportion of those who possessed accurate knowledge regarding working environment management, control of operations and health management was 68.8%, 55.0% and 51.2%, respectively. Experience of radiation decontamination work was associated with the possession of inaccurate knowledge regarding working environment management (OR 0.140 (95% CI 0.042 to 0.464)), and the uncertainty of future radiation decontamination work schedules in difficult-to-return zones was associated with the possession of accurate knowledge regarding health management (OR 4.344 (95% CI 1.509 to 12.50)).ConclusionsPrevious experience in radiation decontamination work may hinder the ability to acquire accurate information regarding working environment management among candidates for an OL position. To promote adequate occupational health management for radiation decontamination workers, it is required to establish an effective instructional method for the OL candidate training sessions with consideration of previous relevant experience.
Published: 2019

27. Hypopharyngeal Diverticulum: Toward a Unified Understanding of Its Etiopathogenesis

Author: Sina J. Torabi, Howard P. Boey, Clarence T. Sasaki, and David A. Kasle
Subjects: Myotomy, Zenker Diverticulum, medicine.medical_treatment, Cricopharyngeus Muscle, digestive system, 030507 speech-language pathology & audiology, 03 medical and health sciences, Speech and Hearing, Zenker's diverticulum, 0302 clinical medicine, Muscle Hypertonia, otorhinolaryngologic diseases, medicine, Pressure, Humans, Esophagus, Raphe, medicine.diagnostic_test, business.industry, Gastroenterology, Anatomy, Pharyngeal Diseases, medicine.disease, Esophageal Sphincter, Upper, digestive system diseases, Endoscopy, Diverticulum, Hypopharynx, medicine.anatomical_structure, Otorhinolaryngology, Gastroesophageal Reflux, Pharyngeal Muscles, Sphincter, 0305 other medical science, business, 030217 neurology & neurosurgery
Abstract: The etiopathogenesis of Zenker's diverticulum (ZD) remains uncertain. Increased hypopharyngeal pressure due to a hypertonic upper esophageal sphincter results in herniation proximal to the sphincter producing a pulsion diverticulum. Gastroesophageal reflux, which is known to induce shortening of the injured esophagus, likely plays a prominent role in ZD formation by pulling the cricopharyngeus muscle (CPM) away from the anchored inferior constrictor muscle. This creates a "weak zone" encouraging herniation. A bilobed diverticulum may originate from continuation of the fibrous midline raphe inferiorly to developmentally include part of the CPM. We report using laser endoscopy to divide the inter-diverticular septum followed by transmucosal cricopharyngeus myotomy. Presentation of a rare, bilobed diverticulum emphasizes the importance of the midline prevertebral raphe in anchoring the pharyngeal constrictor muscles with respect to the CPM. This lends support to the hypothesis that the etiopathogenesis of ZD is multifactorial while guiding us to a unified understanding of ZD.
Published: 2019

28. Biliary reflux as a causal factor in hypopharyngeal carcinoma: New clinical evidence and implications

Author: Sotirios G. Doukas, Dimitra P. Vageli, Jose Costa, and Clarence T. Sasaki
Subjects: Male, Cancer Research, medicine.disease_cause, Hypopharyngeal Carcinoma, Bile reflux, Bile Acids and Salts, 03 medical and health sciences, Laryngopharyngeal reflux, Mice, 0302 clinical medicine, Carcinoma, Medicine, Animals, Bile, Humans, 030212 general & internal medicine, RNA, Messenger, Aged, Hypopharyngeal Neoplasms, Mucous Membrane, business.industry, Bile Reflux, NF-kappa B, Hypopharyngeal cancer, Oncomir, Middle Aged, medicine.disease, Neoplasm Proteins, Gene Expression Regulation, Neoplastic, MicroRNAs, Real-time polymerase chain reaction, Oncology, 030220 oncology & carcinogenesis, Cancer research, Carcinoma, Squamous Cell, Female, business, Carcinogenesis
Abstract: Background Recent preclinical explorations strongly support the tumorigenic potential of bile on laryngopharyngeal mucosa. Herein, the authors describe, in bile-related human hypopharyngeal squamous cell carcinoma (HSCC), NF-κB-related messenger RNA (mRNA) and microRNA (miRNA) oncogenic phenotypes similar to those previously identified in acidic bile-exposed premalignant murine hypopharyngeal mucosa. Methods In this pilot study, the authors included human HSCC specimens paired with their adjacent normal tissue (ANT) derived from 3 representative patients with documented biliary laryngopharyngeal reflux (bile[+]) compared with 5 control patients without signs of bile reflux disease (bile[-]). Immunohistochemical, quantitative polymerase chain reaction, and miRNA analyses were used to detect the levels of activated NF-κB and expression levels of STAT3, EGFR, BCL2, WNT5A, IL-6, IL-1B, ΔNp63, cREL, TNF-α, TP53, NOTCH1, NOTCH2, NOTCH3, miR-21, miR-155, miR-192, miR-34a, miR-375, miR-451a, miR-489, miR-504, and miR-99a. Results Bile(+) HSCC demonstrated an intense NF-κB activation accompanied by significant overexpression of RELA(p65), EGFR, STAT3, BCL-2, cREL, ΔNp63, WNT5A, IL-6, and IL1B; upregulation of oncomir miR-21; and downregulation of tumor suppressor miR-375 compared with their respective ANTs. Bile(+) HSCC demonstrated significantly higher mRNA levels of all the analyzed genes, particularly RELA(p65), IL-6, EGFR, and TNF-α compared with bile(-) tumors. The miR-21/miR-375 ratio, which previously has been linked to tumor aggressiveness, was found to be >260-fold and >30-fold higher, respectively, in bile(+) HSCCs compared with their ANTs and bile(-) tumors. Conclusions Although limitations apply to this pilot study due to the small number of patients with HSCC, the novel findings suggest that a history of bile as a component of esophageal reflux disease may represent an independent risk factor for hypopharyngeal carcinogenesis.
Published: 2019

29. HPV-Positive Oropharyngeal Cancer Via p16 Immunohistochemistry in Japan

Author: Hisashi Hasegawa, Takashi Kurita, Scott Von Larson, Hirohito Umeno, Tohru Furusaka, Julia Toman, Manju L. Prasad, and Clarence T. Sasaki
Subjects: Male, Oncology, medicine.medical_specialty, HPV-positive oropharyngeal cancer, 03 medical and health sciences, 0302 clinical medicine, Japan, Internal medicine, medicine, Humans, Sex Distribution, Stage (cooking), 030223 otorhinolaryngology, Cyclin-Dependent Kinase Inhibitor p16, Aged, P16 immunohistochemistry, Squamous Cell Carcinoma of Head and Neck, business.industry, Papillomavirus Infections, Smoking, Head and neck cancer, Cancer, General Medicine, Middle Aged, medicine.disease, Immunohistochemistry, Head and neck squamous-cell carcinoma, Oropharyngeal Neoplasms, Otorhinolaryngology, Head and Neck Neoplasms, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Biomarker (medicine), Female, Smoking status, business
Abstract: Objectives: Human papillomavirus (HPV) has emerged as a driving cause of head and neck cancer, but investigations outside the West are limited. A p16 immunohistochemistry is a commonly used biomarker for HPV cancers. We sought to investigate the pathology and rates of HPV head and neck oropharyngeal cancer in Japan via p16 immunohistochemistry at 2 institutions in Japan. Methods: Fifty-nine oropharyngeal specimens from 2 university hospitals in Japan were examined for morphology and p16 immunohistochemistry. The rate of p16 positivity was then determined, and the 2 groups were compared for differences in age, smoking history, gender, and stage of presentation and mortality. Results: The rate of p16 positivity among the oropharyngeal specimens was 29.5%. There were important differences in the pathology compared to morphology usually seen in the US. The patients with p16+ cancer tended to be younger. There was no significant difference in smoking status. Patients with p16+ cancers trended toward better survival. Conclusion: There appears to be a geographical difference in HPV rates of oropharyngeal cancers with persistently lower rates in Asian countries when compared to Western Europe and the US. Conclusions about HPV head and neck squamous cell carcinoma (HNSCC) in Western countries may not be generalizable across the globe at this time.
Published: 2016
Full Text: View/download PDF

30. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways

Author: Bruce J. Tromberg, Seong M. Kim, Robert Edwards, Stephen Hanessian, Ryan J. McMonigle, Garret Guenther, Aimee L. Edinger, Tricia T. Nguyen, Eric O. Potma, Brendan T. Finicle, Yanling Zhang, Bin Chen, Tiffany M. Nguyen, Jue Hou, Atsuo T. Sasaki, Archna Ravi, Satoshi Kofuji, Mari Kono, Lois S. Weisman, Alison N. McCracken, Saurabh G. Roy, Manuel U. Ramirez, Timothy Wiher, and Elizabeth Selwan
Subjects: 0301 basic medicine, Autophagosome, media_common.quotation_subject, Biological Transport, Active, Enzyme Activators, Mice, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, PIKFYVE, Phosphatidylinositol Phosphates, Cell Line, Tumor, Neoplasms, Medicine and Health Sciences, Animals, Humans, Protein Phosphatase 2, Macropinosome, Internalization, media_common, Mice, Knockout, 2. Zero hunger, Sphingolipids, Kinase, Chemistry, Autophagy, General Medicine, Protein phosphatase 2, Neoplasm Proteins, Cell biology, 030104 developmental biology, Biochemistry, Cancer cell, Drug Screening Assays, Antitumor, Research Article
Abstract: Oncogenic mutations drive anabolic metabolism, creating a dependency on nutrient influx through transporters, receptors, and macropinocytosis. While sphingolipids suppress tumor growth by downregulating nutrient transporters, macropinocytosis and autophagy still provide cancer cells with fuel. Therapeutics that simultaneously disrupt these parallel nutrient access pathways have potential as powerful starvation agents. Here, we describe a water-soluble, orally bioavailable synthetic sphingolipid, SH-BC-893, that triggers nutrient transporter internalization and also blocks lysosome-dependent nutrient generation pathways. SH-BC-893 activated protein phosphatase 2A (PP2A), leading to mislocalization of the lipid kinase PIKfyve. The concomitant mislocalization of the PIKfyve product PI(3,5)P2 triggered cytosolic vacuolation and blocked lysosomal fusion reactions essential for LDL, autophagosome, and macropinosome degradation. By simultaneously limiting access to both extracellular and intracellular nutrients, SH-BC-893 selectively killed cells expressing an activated form of the anabolic oncogene Ras in vitro and in vivo. However, slower-growing, autochthonous PTEN-deficient prostate tumors that did not exhibit a classic Warburg phenotype were equally sensitive. Remarkably, normal proliferative tissues were unaffected by doses of SH-BC-893 that profoundly inhibited tumor growth. These studies demonstrate that simultaneously blocking parallel nutrient access pathways with sphingolipid-based drugs is broadly effective and cancer selective, suggesting a potential strategy for overcoming the resistance conferred by tumor heterogeneity.
Published: 2016
Full Text: View/download PDF

31. Time course of osteoporotic vertebral fractures by magnetic resonance imaging using a simple classification: a multicenter prospective cohort study

Author: Fumiaki Kanematsu, Kazushi Takayama, Ryuichi Sasaoka, Hiroyuki Yasuda, Tadao Tsujio, Hiroaki Nakamura, Masatoshi Hoshino, Shinji Takahashi, Hiroshi Kono, Hiromitsu Toyoda, T. Sasaki, and K. Iseki
Subjects: Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Inversion recovery, 03 medical and health sciences, 0302 clinical medicine, Image Interpretation, Computer-Assisted, Humans, Medicine, Prospective Studies, 030212 general & internal medicine, Prospective cohort study, Aged, Aged, 80 and over, Fracture Healing, Observer Variation, medicine.diagnostic_test, business.industry, Reproducibility of Results, Magnetic resonance imaging, Prognosis, Compression (physics), Magnetic Resonance Imaging, Vertebral body, Vertebral height, Orthopedic surgery, Time course, Spinal Fractures, Female, Radiology, business, Nuclear medicine, Osteoporotic Fractures, 030217 neurology & neurosurgery, Follow-Up Studies
Abstract: This study revealed the time course of osteoporotic vertebral fracture by magnetic resonance imaging using a simple classification. Signal changes were associated with the compression degree and mobility of the fractured vertebral body. This classification showed sufficient reliability in categorizing magnetic resonance imaging findings of osteoporotic vertebral fractures. Magnetic resonance imaging (MRI) is useful in diagnosing osteoporotic vertebral fractures (OVFs). This study investigated the time course of OVFs by MRI using a simple classification. This multicenter cohort study was performed from 2012 to 2015. Consecutive patients with ≤2-week-old OVFs were enrolled in 11 institutions. MRI was performed at enrollment and at 1-, 3-, 6-, and 12-month follow-up. Signal changes on T1-weighted imaging (T1WI), T2WI, and short τ inversion recovery (STIR) were classified according to signal intensity. Height and angular motion of vertebral bodies were also measured. The 6-month follow-up was completed by 153 patients. At enrollment, fractured vertebrae signal changes were 43 % diffuse and 57 % confined low on T1WI; on T2WI, 56, 24, and 5 % were confined low, high, and diffuse low, respectively; on STIR, 100 % were high. On T1WI, diffuse low remained most common (90 % at 1 month and 60 % at 3 months) until 6 and 12 months, when most were confined low (54 and 52 %, respectively). On T2WI, confined low remained most common (decreasing to 41 % at 12 months). On STIR, high signal change was shown in 98, 87, and 64 % at 3, 6, and 12 months, respectively. At 3, 6, and 12 months, diffuse low signal change was associated with significantly lower vertebral height, and high signal change was associated with significantly greater angular motion. MRI signal changes were associated with the compression degree and angular motion of fractured vertebrae. This classification showed sufficient reliability in categorizing MRI findings of OVFs.
Published: 2016
Full Text: View/download PDF

32. The role of prosody and context in sarcasm comprehension: Behavioral and fMRI evidence

Author: Hiroki C. Tanabe, Akira Utsumi, Norihiro Sadato, Akihiro T. Sasaki, Yumiko Yoshida, Tomoko Matsui, Takahiko Koike, Tagiru Nakamura, and Tokiko Harada
Subjects: Adult, Male, media_common.quotation_subject, Cognitive Neuroscience, Emotions, Functional magnetic resonance imaging, Context (language use), Experimental and Cognitive Psychology, Prosody, Neuropsychological Tests, 050105 experimental psychology, Developmental psychology, 03 medical and health sciences, Judgment, Young Adult, Behavioral Neuroscience, 0302 clinical medicine, Perception, Humans, 0501 psychology and cognitive sciences, Praise, media_common, Language, Modulation, Brain Mapping, Sarcasm, Language Tests, 05 social sciences, Brain, Recognition, Psychology, Inferior frontal gyrus, Magnetic Resonance Imaging, Comprehension, Pattern Recognition, Visual, Speech Perception, Female, Incongruity, Psychology, Insula, 030217 neurology & neurosurgery, Utterance, psychological phenomena and processes, Cognitive psychology, Wit and Humor as Topic
Abstract: A hearer's perception of an utterance as sarcastic depends on integration of the heard statement, the discourse context, and the prosody of the utterance, as well as evaluation of the incongruity among these aspects. The effect of prosody in sarcasm comprehension is evident in everyday conversation, but little is known about its underlying mechanism or neural substrates. To elucidate the neural underpinnings of sarcasm comprehension in the auditory modality, we conducted a functional MRI experiment with 21 adult participants. The participants were provided with a short vignette in which a child had done either a good or bad deed, about which a parent made a positive comment. The participants were required to judge the degree of the sarcasm in the parent's positive comment (praise), which was accompanied by either positive or negative affective prosody. The behavioral data revealed that an incongruent combination of utterance and the context (i.e., the parent's positive comment on a bad deed by the child) induced perception of sarcasm. There was a significant interaction between context and prosody: sarcasm perception was enhanced when positive prosody was used in the context of a bad deed or, vice versa, when negative prosody was used in the context of a good deed. The corresponding interaction effect was observed in the rostro-ventral portion of the left inferior frontal gyrus corresponding to Brodmann's Area (BA) 47. Negative prosody incongruent with a positive utterance (praise) activated the bilateral insula extending to the right inferior frontal gyrus, anterior cingulate cortex, and brainstem. Our findings provide evidence that the left inferior frontal gyrus, particularly BA 47, is involved in integration of discourse context and utterance with affective prosody in the comprehension of sarcasm.
Published: 2016
Full Text: View/download PDF

33. Predicting delayed union in osteoporotic vertebral fractures with consecutive magnetic resonance imaging in the acute phase: a multicenter cohort study

Author: Kazushi Takayama, Hiroaki Nakamura, Fumiaki Kanematsu, Shinji Takahashi, T. Sasaki, Hiroshi Kono, Tadao Tsujio, K. Iseki, Masatoshi Hoshino, Hiromitsu Toyoda, Ryuichi Sasaoka, and Hiroyuki Yasuda
Subjects: Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Radiography, Osteoporosis, Sensitivity and Specificity, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Predictive Value of Tests, Internal medicine, medicine, Humans, Aged, Aged, 80 and over, 030222 orthopedics, medicine.diagnostic_test, business.industry, Magnetic resonance imaging, medicine.disease, Magnetic Resonance Imaging, Spine, Rheumatology, Fractures, Ununited, Predictive value of tests, Radiological weapon, Orthopedic surgery, Spinal Fractures, Female, sense organs, Radiology, business, Osteoporotic Fractures, 030217 neurology & neurosurgery, Cohort study
Abstract: This study demonstrated the predictive values of radiological findings for delayed union after osteoporotic vertebral fractures (OVFs). High-signal changes on T2WI were useful findings.The purpose of the present study is to determine predictive radiological findings for delayed union by magnetic resonance imaging (MRI) and plain X-rays at two time points in the acute phase of OVFs.This multicenter cohort study was performed from 2012 to 2015. A total of 218 consecutive patients with OVFs ≤2 weeks old were enrolled. MRIs and plain X-rays were performed at the time of enrollment and at 1- and 6-month follow-ups. Signal changes on T1-weighted imaging (T1WI) were classified as diffuse low-, confined low-, or no-signal change; those on T2WI were classified as high (similar to the intensity of cerebrospinal fluid), confined low-, diffuse low-, or no-signal change. The angular motion of the fractured vertebral body was measured with X-rays.A total of 153 patients completed the 6-month follow-up. A high-signal change on T2WI was most useful in predicting delayed union. Sensitivity, specificity, and positive predictive values were 53.3, 87.8, and 51.6 % at enrollment and 65.5, 84.8, and 51.4 % at the 1-month follow-up, respectively. The positive predictive value increased to 62.5 % with observation of high- or diffuse low-signal changes at both enrollment and the 1-month follow-up. The cutoff value of vertebral motion was 5 degrees. Sensitivity and specificity at enrollment were 52.4 and 74.1 %, respectively.This study demonstrated the radiological factors predicting delayed union after an OVF. T2 high-signal changes showed the strongest association with delayed union. Consecutive MRIs were particularly useful as a differential tool to predict delayed union following OVFs.
Published: 2016
Full Text: View/download PDF

34. miR-21, miR-155, miR-192, and miR-375 Deregulations Related to NF-kappaB Activation in Gastroduodenal Fluid–Induced Early Preneoplastic Lesions of Laryngeal Mucosa In Vivo

Author: Dimitra P. Vageli and Clarence T. Sasaki
Subjects: 0301 basic medicine, Original article, Cancer Research, Pathology, medicine.medical_specialty, Biology, Polymerase Chain Reaction, lcsh:RC254-282, Duodenogastric Reflux, miR-155, Mice, 03 medical and health sciences, 0302 clinical medicine, In vivo, microRNA, medicine, Animals, Bile, Humans, Neoplastic transformation, Cell Proliferation, Gastric Juice, Cell growth, Cell adhesion molecule, Transcription Factor RelA, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Enzyme Activation, Gene Expression Regulation, Neoplastic, Mice, Inbred C57BL, MicroRNAs, 030104 developmental biology, Real-time polymerase chain reaction, Laryngeal Mucosa, 030220 oncology & carcinogenesis, Models, Animal, Gastroesophageal Reflux, Cancer research, Immunohistochemistry, Precancerous Conditions
Abstract: Gastroduodenal refluxate found in the upper aerodigestive tract is not clinically uncommon. We recently demonstrated the neoplastic potential of gastroduodenal fluids (GDF) on hypopharyngeal mucosa, via NF-κB, using in vitro and in vivo models. Here we will explore the in vivo effect of GDF on laryngeal mucosa (LM) to induce early preneoplastic lesions related to NF-κB activation, along with deregulation of specific microRNA (miRNA) markers previously linked to laryngeal cancer. We used histological, immunohistochemical, automated quantitative analysis and quantitative polymerase chain reaction to examine LM from 35 C57Bl/6J mice previously treated with topical GDF against corresponding controls (4 experimental and 3 control groups; 5 mice/group). Our analysis showed that GDF produced early preneoplastic lesions in treated LM related to NF-κB activation. LM treated by acid and bile combination demonstrated significantly higher expression of the analyzed cell proliferation markers (Ki67, CK14, ∆Np63), oncogenic p-STAT3, and changes of cell adhesion molecules (E-cadherin, ϐ-catenin) versus untreated LM or LM exposed to acid alone (P < .0005). Furthermore, acidic bile but not neutral bile appeared to accelerate the expression of “oncomirs” miR-21, miR-155, and miR-192 (acidic bile versus neutral bile, P < .0001), while reducing tumor suppressor miR-375 (acidic bile versus neutral bile, P = .0137), previously linked to NF-κB and laryngeal cancer. Finally, acidic bile induced reduction of miR-34a, miR-375, and miR-451a, exhibiting an inverse correlation with NF-κB activation. SIGNIFICANCE: Bile in combination with acid has a selective tumorigenic effect on LM, inducing deregulation of “oncomirs” and tumor suppressor miRNAs, produced by NF-κB activation with molecular and early histopathological alterations linked to neoplastic transformation. Systematic acid suppression may in part convey a protective role.
Published: 2016
Full Text: View/download PDF

35. Clinical efficacy and safety of topiroxostat in Japanese male hyperuricemic patients with or without gout: an exploratory, phase 2a, multicentre, randomized, double‐blind, placebo‐controlled study

Author: Tatsuo Hosoya, T Sasaki, R. Sakamoto, T Ohashi, and H. Hashimoto
Subjects: Adult, Male, Xanthine Oxidase, medicine.medical_specialty, Gout, Pyridines, 030232 urology & nephrology, Placebo-controlled study, Hyperuricemia, 030204 cardiovascular system & hematology, Placebo, Gout Suppressants, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Double-Blind Method, Japan, Internal medicine, Nitriles, medicine, Humans, Pharmacology (medical), Adverse effect, Pharmacology, Dose-Response Relationship, Drug, business.industry, Middle Aged, medicine.disease, Confidence interval, Uric Acid, Surgery, Topiroxostat, Treatment Outcome, chemistry, Uric acid, business, Follow-Up Studies
Abstract: Summary What is known and objective In Japan, although topiroxostat, a selective xanthine oxidoreductase inhibitor, has been used for the treatment of patients with hyperuricemia including gout, no published randomized controlled studies evaluating the dose-dependent relationship with respect to the serum urate-lowering efficacy have been reported. The aim of this study was to evaluate the dose-dependent relationship with serum urate-lowering efficacy and safety of topiroxostat in Japanese hyperuricemic patients including gout. Methods We conducted an exploratory, phase 2a, multicentre, randomized, double-blind, 8-week, placebo-controlled study in Japanese hyperuricemic patients with or without gout. The study arms were placebo and topiroxostat 40, 60, 80 or 120 mg/day. The primary efficacy endpoint was the per cent change in serum urate level from baseline to the final visit. Results and discussion One hundred and eighty-seven eligible patients were randomized and 186 received at least one dose of the study drug. The study results demonstrated a dose-dependent serum urate reduction effect ranging from 40 to 120 mg/day (P < 0·001, Jonckheere–Terpstra test). The mean per cent change in serum urate level from baseline at the final visit was −30·8% in the 120-mg group and 1·6% with placebo, with a between-group difference of −32·4% ([95% confidence interval, −38·9% to −25·9%]; P < 0·001). Incidences of overall adverse events (AEs) in the topiroxostat groups were comparable to those in the placebo group; however, the incidence of AEs in the 120-mg group was statistically lower than that in the placebo group. The incidences of gouty arthritis were not statistically but numerically higher in the topiroxostat 80- and 120-mg groups. What is new and conclusions A dose-dependent serum urate-lowering efficacy of topiroxostat was observed in Japanese hyperuricemic male patients with or without gout. Further clinical studies aimed at evaluating the long-term safety and clinical efficacy are warranted.
Published: 2016
Full Text: View/download PDF

36. Pedicle versus free flap reconstruction in patients receiving intraoperative brachytherapy

Author: Deepak Narayan, Yung Son, Bryce A. Basques, Christopher C. Chang, Erik J. Geiger, Stephan Ariyan, Clarence T. Sasaki, and Andrew McGregor
Subjects: Male, Reoperation, medicine.medical_specialty, medicine.medical_treatment, Brachytherapy, Free flap, Free Tissue Flaps, Surgical Flaps, 03 medical and health sciences, Postoperative Complications, 0302 clinical medicine, Humans, Medicine, 030223 otorhinolaryngology, Aged, Retrospective Studies, Pedicle flap, Intraoperative Care, business.industry, Head and neck cancer, Retrospective cohort study, Middle Aged, medicine.disease, Combined Modality Therapy, eye diseases, Surgery, Logistic Models, Head and Neck Neoplasms, 030220 oncology & carcinogenesis, Multivariate Analysis, Free flap reconstruction, Female, business, Complication
Abstract: Introduction This study compared complication rates between pedicle flaps and free flaps used for resurfacing of intraoperative brachytherapy (IOBT) implants placed following head and neck tumour extirpation to help clarify the ideal reconstructive procedure for this scenario. Patients and methods A retrospective review of reconstructions with IOBT at our institution was conducted. Patient and treatment details were recorded, as were the number and type of flap complications, including re-operations. Logistic regressions compared complications between flap groups. Results Fifty free flaps and 55 pedicle flaps were included. On multivariate analysis, free flap reconstruction with IOBT was significantly associated with both an increased risk of having any flap complication (OR = 2.9, p = 0.037) and with need for operative revision (OR = 3.5, p = 0.048) compared to pedicle flap reconstruction. Conclusions In the setting of IOBT, free flaps are associated with an increased risk of having complications and requiring operative revisions.
Published: 2016
Full Text: View/download PDF

37. Evaluation of PD-L1 Expression and Associated Tumor-Infiltrating Lymphocytes in Laryngeal Squamous Cell Carcinoma

Author: Aris I. Giotakis, Christos Perisanidis, Christos K. Kontos, Vassiliki Kotoula, Maria Vassilakopoulou, Amanda Psyrri, David L. Rimm, Kyriakos Chatzopoulos, Clarence T. Sasaki, Vamsidhar Velcheti, Theodore Rampias, George Fountzilas, Margaritis Avgeris, and Andreas Scorilas
Subjects: Adult, Male, 0301 basic medicine, Cancer Research, Pathology, medicine.medical_specialty, Stromal cell, Cell, H&E stain, Gene Expression, Kaplan-Meier Estimate, B7-H1 Antigen, 03 medical and health sciences, Lymphocytes, Tumor-Infiltrating, 0302 clinical medicine, Risk Factors, Gene expression, Biomarkers, Tumor, Carcinoma, Humans, Medicine, RNA, Messenger, Neoplasm Metastasis, Laryngeal Neoplasms, Aged, Neoplasm Staging, Proportional Hazards Models, Retrospective Studies, Aged, 80 and over, Tissue microarray, business.industry, Tumor-infiltrating lymphocytes, Middle Aged, Prognosis, medicine.disease, Immunohistochemistry, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Female, Neoplasm Grading, business, Follow-Up Studies
Abstract: Purpose: Programmed death-ligand 1 (PD-L1; also known as CD274 or B7-H1) expression represents a mechanism of immune escape for cancer. Our purpose was to characterize tumor PD-L1 expression and associated T-cell infiltration in primary laryngeal squamous cell carcinomas (SCC). Experimental Design: A well-annotated cohort of 260 operable primary laryngeal SCCs [formalin-fixed paraffin-embedded (FFPE) specimens] was morphologically characterized for stromal tumor-infiltrating lymphocytes (TIL), on hematoxylin/eosin-stained whole sections and for PD-L1 mRNA expression by qRT-PCR in FFPE specimens. For PD-L1 protein expression, automated quantitative protein analysis (AQUA) was applied on tissue microarrays consisting of two cores from these tumors. In addition, PD-L1 mRNA expression in fresh-frozen tumors and normal adjacent tissue specimens was assessed in a second independent cohort of 89 patients with primary laryngeal SCC. Results: PD-L1 mRNA levels were upregulated in tumors compared with surrounding normal tissue (P = 0.009). TILs density correlated with tumor PD-L1 AQUA levels (P = 0.021). Both high TILs density and high PD-L1 AQUA levels were significantly associated with superior disease-free survival (DFS; TILs: P = 0.009 and PD-L1: P = 0.044) and overall survival (OS; TILs: P = 0.015 and PD-L1: P = 0.059) of the patients and retained significance in multivariate analysis. Conclusions: Increased TILs density and PD-L1 levels are associated with better outcome in laryngeal squamous cell cancer. Assessment of TILs and PD-L1 expression could be useful to predict response to immune checkpoint inhibitors. Clin Cancer Res; 22(3); 704–13. ©2015 AACR.
Published: 2016
Full Text: View/download PDF

38. Gastro-duodenal fluid induced nuclear factor-κappaB activation and early pre-malignant alterations in murine hypopharyngeal mucosa

Author: Clarence T. Sasaki, Manju L. Prasad, and Dimitra P. Vageli
Subjects: 0301 basic medicine, Pathology, medicine.medical_specialty, Duodenum, Blotting, Western, Fluorescent Antibody Technique, Real-Time Polymerase Chain Reaction, NF-κB, Bile Acids and Salts, Immunoenzyme Techniques, Mice, 03 medical and health sciences, 0302 clinical medicine, Hypopharyngeal Neoplasm, In vivo, Animals, Humans, Medicine, RNA, Messenger, STAT3, Cells, Cultured, bile acids, Hypopharyngeal Neoplasms, Mucous Membrane, biology, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Cell adhesion molecule, Cell growth, Stomach, NF-kappa B, Hypopharyngeal cancer, NFKB1, medicine.disease, In vitro, Mice, Inbred C57BL, in vivo, Hypopharynx, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, gastroduodenal reflux, Cancer research, biology.protein, business, hypopharyngeal cancer, Precancerous Conditions, Research Paper
Abstract: We recently described the role of gastro-duodenal fluids (GDFs) in generating changes consistent with hypopharyngeal neoplasia through activation of NF-κB pathway, using an in vitro model of human hypopharyngeal normal keratinocytes. Here, we further provide evidence that gastro-duodenal reflux is a risk factor for early pre-malignant alterations in hypopharyngeal mucosa (HM) related to an activated NF-κB oncogenic pathway, using both an in vitro and a novel in vivo model of C57Bl/6J mice. Histological, immunohistochemical and automated quantitative analysis documents significant NF-κB activation and early pre-malignant alterations in HM topically exposed to GDFs, compared to acid alone and other controls. Early pre-malignant histologic lesions exhibited increased Ki67, CK14 and ΔNp63, cell proliferation markers, changes of cell adhesion molecules, E-Cadherin and β-catenin, and STAT3 activation. The in vivo effect of NF-κB activation is positively correlated with p-STAT3, Ki67, CK14 or β-catenin expression, while GDFs induce significant transcriptional activation of RELA(p65), bcl-2, TNF-α, STAT3, EGFR and wnt5A, in vivo. Our in vivo model demonstrates selectively activated NF-κB in response to topically administrated GDFs, leading to early pre-malignant events in HM.
Published: 2016
Full Text: View/download PDF

39. Neural substrates of shared attention as social memory: A hyperscanning functional magnetic resonance imaging study

Author: Akihiro T. Sasaki, Sho K. Sugawara, Koji Shimada, Haruka K. Takahashi, Jorge Bosch-Bayard, Hiroki C. Tanabe, Eri Nakagawa, Takahiko Koike, Shuntaro Okazaki, Kazufumi Yoshihara, and Norihiro Sadato
Subjects: Male, Joint attention, genetic structures, Cognitive Neuroscience, Fixation, Ocular, 050105 experimental psychology, Task (project management), 03 medical and health sciences, Young Adult, 0302 clinical medicine, Memory, Synchronization (computer science), medicine, Humans, 0501 psychology and cognitive sciences, Attention, Interpersonal Relations, Association (psychology), Hyperscanning, medicine.diagnostic_test, 05 social sciences, Eye-blink synchronization, Inter-individual neural synchronization, Brain, Shared attention, Gaze, Magnetic Resonance Imaging, Social relation, Hebbian theory, Neurology, Female, Functional magnetic resonance imaging, Psychology, 030217 neurology & neurosurgery, Cognitive psychology, Mutual gaze
Abstract: During a dyadic social interaction, two individuals can share visual attention through gaze, directed to each other (mutual gaze) or to a third person or an object (joint attention). Shared attention is fundamental to dyadic face-to-face interaction, but how attention is shared, retained, and neutrally represented in a pair-specific manner has not been well studied. Here, we conducted a two-day hyperscanning functional magnetic resonance imaging study in which pairs of participants performed a real-time mutual gaze task followed by a joint attention task on the first day, and mutual gaze tasks several days later. The joint attention task enhanced eye-blink synchronization, which is believed to be a behavioral index of shared attention. When the same participant pairs underwent mutual gaze without joint attention on the second day, enhanced eye-blink synchronization persisted, and this was positively correlated with inter-individual neural synchronization within the right inferior frontal gyrus. Neural synchronization was also positively correlated with enhanced eye-blink synchronization during the previous joint attention task session. Consistent with the Hebbian association hypothesis, the right inferior frontal gyrus had been activated both by initiating and responding to joint attention. These results indicate that shared attention is represented and retained by pair-specific neural synchronization that cannot be reduced to the individual level.
Published: 2016
Full Text: View/download PDF

40. The role of bile reflux and its related NF-κB activated pathway in progression of hypopharyngeal squamous cell cancer

Author: Clarence T. Sasaki, Dimitra P. Vageli, Michael Hajek, and Sotirios G. Doukas
Subjects: Cancer Research, medicine.disease_cause, Bile reflux, 03 medical and health sciences, Laryngopharyngeal reflux, chemistry.chemical_compound, 0302 clinical medicine, Cell Line, Tumor, medicine, Humans, Neoplasms, Squamous Cell, 030223 otorhinolaryngology, STAT3, Hypopharyngeal Neoplasms, biology, business.industry, Bile Reflux, NF-kappa B, Hypopharyngeal cancer, NF-κB, Prognosis, medicine.disease, Blot, Oncology, chemistry, Cell culture, 030220 oncology & carcinogenesis, Disease Progression, biology.protein, Cancer research, Oral Surgery, Carcinogenesis, business
Abstract: Background Prognosis for hypopharyngeal cancer is usually poor, and recurrence is common. Identifying new factors or related mechanisms that promote its progression may have clinical implications. Although, recent studies support bile reflux in hypopharyngeal carcinogenesis, it remains to be explored how bile and its related NF-κB activated pathway may further affects its progression in already established hypopharyngeal cancer. Methods Hypopharyngeal squamous cell carcinoma (HSCC) cell lines, FaDu and UMSCC11A, both negative for HPV, were repetitively exposed to bile acids (400 μM) at variable pH points (4.0, 5.5 and 7.0). Immunofluorescence, western blotting, luciferase assay, and qPCR were used to detect NF-κB activation, bcl-2 overexpression and gene expression. Results Bile at strongly acidic pH (4.0) potentiated the activation of NF-κB and its related mRNA phenotype in HSCC cells. IL-6, TNF-α, and BCL2 were found among the highest overexpressed genes as was previously found in HSCCs excised from patients with documented biliary reflux. An enhanced transcriptional activity of EGFR, RELA, STAT3, and WNT5Α and higher survival rates were observed in HSCC cells exposed to acidic bile compared to those exposed to bile at weakly acidic or neutral pH. Conclusion Our novel findings support the observation that bile reflux has the potential for actively influencing the progression of hypopharyngeal cancer, mediated by NF-κB. In patients with hypopharyngeal cancer and known gastroesophageal reflux disease, antacid therapy may exert a role in furthering control of disease recurrence and progression.
Published: 2020
Full Text: View/download PDF

41. A Clinical Care Pathway to Reduce ICU Usage in Head and Neck Microvascular Reconstruction

Author: Tracy Carafeno, Saral Mehra, Benjamin L. Judson, Wendell G. Yarbrough, Elliot Morse, Clarence T. Sasaki, Jacqueline Dibble, Cara M. Henderson, Peter Longley, and Edwin Chan
Subjects: Male, medicine.medical_specialty, Critical Care, Free Tissue Flaps, Patient Readmission, 03 medical and health sciences, 0302 clinical medicine, Postoperative Complications, Intensive care, medicine, Humans, Clinical care, 030223 otorhinolaryngology, Intensive care medicine, Head and neck, Aged, Retrospective Studies, Postoperative Care, business.industry, Head and neck cancer, Length of Stay, Middle Aged, Plastic Surgery Procedures, medicine.disease, Otorhinolaryngology, Head and Neck Neoplasms, 030220 oncology & carcinogenesis, Critical Pathways, Surgery, Female, business, Cost of care, Facilities and Services Utilization
Abstract: To design and implement a postoperative clinical care pathway designed to reduce intensive care usage on length of stay, readmission rates, and surgical complications in head and neck free flap patients.A postoperative clinical care pathway detailing timelines for patient care was developed by a multispecialty team. In total, 108 matched patients receiving free tissue transfer for reconstruction of head and neck defects in the year before (prepathway), year after (early pathway), and second year after (late pathway) pathway implementation were compared based on postoperative length of stay, 30-day readmission rate, intensive care unit (ICU) admission, and rates of medical/surgical complications.Median length of stay decreased from 10 to 7.5 and 7 days in the pre-, early, and late-pathway groups, respectively ( P = .012). Readmission rate decreased from 16% in the prepathway group to 0% and 3% in the early and late-pathway groups. The number of patients admitted to the ICU postoperatively decreased from 100% to 36% and 6% in the pre-, early, and late-pathway groups, respectively ( P = .025). The rates of surgical and medical complications were equivalent.This pathway effectively reduced ICU admission, length of stay, and readmission rates, without increasing postoperative complications. These outcomes were sustainable over 2 years.Free flap patients may not require routine ICU admission and may be taken off ventilatory support in the operating room. This effectively reduces costly resource use in this patient population. Similar pathways could be introduced at other institutions.
Published: 2018

42. Curcumin prevents the bile reflux-induced NF-κB-related mRNA oncogenic phenotype, in human hypopharyngeal cells

Author: Clarence T. Sasaki, Todd Spock, Dimitra P. Vageli, and Sotirios G. Doukas
Subjects: 0301 basic medicine, gastroesophageal reflux, Bile reflux, chemistry.chemical_compound, 0302 clinical medicine, STAT3, biology, medicine.diagnostic_test, NF-kappa B, Hydrogen-Ion Concentration, Hedgehog signaling pathway, ErbB Receptors, Phenotype, Proto-Oncogene Proteins c-bcl-2, 030220 oncology & carcinogenesis, Molecular Medicine, Original Article, Signal Transduction, STAT3 Transcription Factor, Curcumin, Primary Cell Culture, bile, Wnt-5a Protein, Bile Acids and Salts, 03 medical and health sciences, Western blot, In vivo, medicine, Anticarcinogenic Agents, Humans, Luciferase, RNA, Messenger, Interleukin-6, Tumor Necrosis Factor-alpha, NF‐κB, Transcription Factor RelA, NF-κB, Epithelial Cells, Cell Biology, Original Articles, medicine.disease, Proto-Oncogene Proteins c-rel, Hypopharynx, 030104 developmental biology, chemistry, Gene Expression Regulation, Cancer research, biology.protein, head and neck cancer
Abstract: The presence of bile is not an uncommon finding in acidic oesophageal and extra‐oesophageal refluxate, possibly affecting the hypopharyngeal mucosa and leading to neoplastic events. We recently demonstrated that acidic bile (pH ≤ 4.0) can induce NF‐κB activation and oncogenic mRNA phenotype in normal hypopharyngeal cells and generate premalignant changes in treated hypopharyngeal mucosa. We hypothesize that curcumin, a dietary inhibitor of NF‐κB, may effectively inhibit the acidic bile‐induced cancer‐related mRNA phenotype, in treated human hypopharyngeal primary cells (HHPC), supporting its potential preventive use in vivo. Luciferase assay, immunofluorescence, Western blot, qPCR and PCR microarray analysis were used to explore the effect of curcumin in HHPC exposed to bile (400 μmol/L) at acidic and neutral pH. Curcumin successfully inhibited the acidic bile‐induced NF‐κB signalling pathway (25% of analysed genes), and overexpression of NF‐κB transcriptional factors, c‐REL, RELA(p65), anti‐apoptotic bcl‐2, oncogenic TNF‐α, EGFR, STAT3, WNT5A, ΔNp63 and cancer‐related IL‐6. Curcumin effectively reduced bile‐induced bcl‐2 overexpression at both acidic and neutral pH. Our novel findings suggest that, similar to pharmacologic NF‐κB inhibitor, BAY 11‐7082, curcumin can suppress acidic bile‐induced oncogenic mRNA phenotype in hypopharyngeal cells, encouraging its future in vivo pre‐clinical and clinical explorations in prevention of bile reflux‐related pre‐neoplastic events mediated by NF‐κB.
Published: 2018

43. Hidden osteophyte formation on plain X-ray is the predictive factor for development of knee osteoarthritis after 48 months – data from the Osteoarthritis Initiative

Author: J. Katsuragi, T. Sasho, S. Yamaguchi, Y. Sato, A. Watanabe, R. Akagi, Y. Muramatsu, S. Mukoyama, Y. Akatsu, T. Fukawa, J. Endo, H. Hoshi, Y. Yamamoto, T. Sasaki, and K. Takahashi
Subjects: Male, musculoskeletal diseases, medicine.medical_specialty, Knee Joint, Radiography, Biomedical Engineering, Lateral tibial plateau, Patellofemoral joint, Osteoarthritis, Cohort Studies, Patellofemoral Joint, Rheumatology, medicine, Humans, Orthopedics and Sports Medicine, Longitudinal Studies, Aged, Pre-radiographic osteoarthritis, medicine.diagnostic_test, business.industry, Osteophyte, Magnetic resonance imaging, Middle Aged, Osteoarthritis, Knee, medicine.disease, Magnetic Resonance Imaging, Osteophyte formation, Surgery, Predictive factor, Osteoarthritis Initiative, Disease Progression, Female, Plain x-ray, Intercondylar notch, business, Nuclear medicine
Abstract: SummaryObjectiveTo examine whether the detection of osteophytes anywhere in the knee could serve as a pre-radiographic biomarker for osteoarthritis (OA) development.MethodsBaseline magnetic resonance imaging (MRIs) of 132 participants in the Osteoarthritis Initiative (OAI) were studied. Based on radiographs, 66 knees were assessed as osteoarthritis-free (no-osteoarthritis [NOA], or Kellgren/Lawrence [K/L] severity grade 0/1 both at baseline and 48 months), and another 66 knees were assessed as having radiographic OA changes (pre-radiographic osteoarthritis [PROA], or with K/L grade 0/1 at baseline and grade ≥2 at 48 months). Using baseline MRI data, we examined eight sites of osteophyte formation: the medial and lateral femoral condyle (MFC and LFC, respectively); medial and lateral tibial plateau (MTP and LTP, respectively); medial and lateral facets of the patellofemoral joint (PM and PL, respectively); tibial spine (TS); and femoral intercondylar notch (IC). Knee joint osteophyte size was assessed via the 8-point marginal osteophytes item of the whole-organ magnetic resonance imaging score (WORMS). The frequencies and distributions of osteophytes were compared between groups.ResultsMild-size osteophytes (defined as score ≥2) were observed more frequently at the MFC (P = 0.00278), MTP (P = 0.0046), TS (P = 0.0146), PM (P
Published: 2015
Full Text: View/download PDF

44. National treatment times in oropharyngeal cancer treated with primary radiation or chemoradiation

Author: Elliot Morse, Zain A. Husain, Clarence T. Sasaki, Wendell G. Yarbrough, Saral Mehra, Barbara Burtness, Shayan Cheraghlou, and Benjamin L. Judson
Subjects: Oncology, Male, Cancer Research, medicine.medical_specialty, Time Factors, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Cox proportional hazards regression, medicine, Humans, 030223 otorhinolaryngology, Aged, Retrospective Studies, business.industry, Incidence (epidemiology), Head and neck cancer, Cancer, Chemoradiotherapy, Middle Aged, medicine.disease, Prognosis, Head and neck squamous-cell carcinoma, Treatment characteristics, Survival Analysis, Oropharyngeal Neoplasms, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Papilloma, Female, Oral Surgery, business
Abstract: To characterize treatment delays in oropharyngeal cancer treated with radiation in a national sample, identify factors associated with delays, and associate treatment delays with survival.We included adults in the National Cancer Database treated for oropharyngeal cancer with primary radiation or chemoradiation 2010-2013. We characterized diagnosis-to-treatment initiation, radiation treatment duration, and diagnosis-to-treatment end intervals as medians. We examined delays for association with patient, tumor, and treatment characteristics and with overall survival with multivariable logistic and Cox proportional hazards regression, respectively.4089 patients were included; 12% received radiation alone and 88% chemoradiation. The incidence of human papilloma virus-associated tumors was 64%. Median durations of diagnosis-to-treatment initiation, radiation duration, and diagnosis-to-treatment end were 35, 50, and 87 days, respectively. Human papilloma virus-positive tumors were linked to decreased delays in radiation treatment duration and diagnosis-to-treatment end (OR = 0.72 (0.60-0.85), p 0.001 and OR = 0.79 (0.66-0.95), p = 0.010, respectively). Delays in radiation treatment duration and diagnosis-to-treatment end were negatively associated with overall survival (HR = 1.23 (1.03-1.47), p = 0.024 and 1.24 (1.04-1.48), p = 0.017, respectively). When examined separately, radiation duration remained associated with decreased overall survival in patients with human papilloma virus-negative (HR = 1.29 (1.03-1.63), p = 0.030) but not human papilloma virus-positive tumors (HR = 1.17 (0.89-1.54), p = 0.257).These median durations can serve as national benchmarks. Diagnosis-to-treatment end interval is associated with overall survival in all patients, and radiation treatment duration in patients with human papilloma virus-negative tumors. These intervals could be considered quality indicators for oropharyngeal squamous cell carcinoma treated with primary radiation or chemoradiation.
Published: 2017

45. Treatment guidelines and patterns of care in oral cavity squamous cell carcinoma: Primary surgical resection vs. nonsurgical treatment

Author: Aarti K. Bhatia, Saral Mehra, Barbara Burtness, Zain A. Husain, Clarence T. Sasaki, Wendell G. Yarbrough, Benjamin L. Judson, and Rance J T Fujiwara
Subjects: Surgical resection, Male, Cancer Research, medicine.medical_specialty, Disease, Logistic regression, Health Services Accessibility, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Oral Cavity Squamous Cell Carcinoma, 030223 otorhinolaryngology, Aged, Proportional hazards model, business.industry, Cancer, Middle Aged, medicine.disease, Survival Analysis, Nonsurgical treatment, Surgery, Clinical trial, Oncology, 030220 oncology & carcinogenesis, Practice Guidelines as Topic, Carcinoma, Squamous Cell, Female, Mouth Neoplasms, Oral Surgery, business
Abstract: The 2017 National Comprehensive Cancer Network Clinical Practice Guidelines recommend surgical resection or definitive radiation therapy for early-stage oral cavity malignancies, and surgical resection or multimodality clinical trials for late-stage disease. Few studies have been conducted to identify predictors of choice of treatment modality for oral cavity malignancies.All patients in the National Cancer Data Base (NCDB) diagnosed with oral cavity squamous cell carcinoma (OCSCC) between 1998 and 2011 were identified. Chi-square and binary logistic regression were used to identify factors predictive of surgical or nonsurgical treatment; multiple imputation was used for missing data. Cox proportional hazards models were generated to identify associations between treatment modality and overall survival (OS).Of 23,459 patients, 4139 (17.6%) underwent primary nonsurgical treatment. Among NCDB-registered facilities, there has been a decrease in use of nonsurgical treatment for OCSCC (OR 0.97, p0.001). Older age, non-white race, Medicaid insurance, low income, low education, and later-stage disease were associated with nonsurgical therapy, while patients at academic/research programs were more likely to undergo surgery (OR 0.38, p0.001). Nonsurgical treatment was associated with decreased OS (HR=2.02, p0.001); this was upheld on subgroup analysis of early- and late-stage disease.Use of primary nonsurgical treatment for OCSCC has decreased over time among NCDB-registered facilities and is associated with factors related to access to care. Surgical resection for the primary treatment of oral cavity cancer may be associated with improved OS, though conclusions regarding survival are limited by the non-randomized nature of the data.
Published: 2017

46. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m

Author: Rui, Su, Lei, Dong, Chenying, Li, Sigrid, Nachtergaele, Mark, Wunderlich, Ying, Qing, Xiaolan, Deng, Yungui, Wang, Xiaocheng, Weng, Chao, Hu, Mengxia, Yu, Jennifer, Skibbe, Qing, Dai, Dongling, Zou, Tong, Wu, Kangkang, Yu, Hengyou, Weng, Huilin, Huang, Kyle, Ferchen, Xi, Qin, Bin, Zhang, Jun, Qi, Atsuo T, Sasaki, David R, Plas, James E, Bradner, Minjie, Wei, Guido, Marcucci, Xi, Jiang, James C, Mulloy, Jie, Jin, Chuan, He, and Jianjun, Chen
Subjects: Adenosine, Leukemia, Brain Neoplasms, Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Antineoplastic Agents, Glioma, Glutarates, Proto-Oncogene Proteins c-myc, Jurkat Cells, Mice, HEK293 Cells, Cell Line, Tumor, CCAAT-Enhancer-Binding Proteins, Animals, Humans, RNA Processing, Post-Transcriptional, Signal Transduction
Abstract: R-2-hydroxyglutarate (R-2HG), produced at high levels by mutant isocitrate dehydrogenase 1/2 (IDH1/2) enzymes, was reported as an oncometabolite. We show here that R-2HG also exerts a broad anti-leukemic activity in vitro and in vivo by inhibiting leukemia cell proliferation/viability and by promoting cell-cycle arrest and apoptosis. Mechanistically, R-2HG inhibits fat mass and obesity-associated protein (FTO) activity, thereby increasing global N
Published: 2017

47. A Thirty-Year Experience With Head and Neck Flap Reconstruction

Author: Alexander Sun, Stephan Ariyan, Derek M. Steinbacher, Xiaolu Xu, and Clarence T. Sasaki
Subjects: Adult, Male, Reoperation, medicine.medical_specialty, Adolescent, 030230 surgery, Free Tissue Flaps, Surgical Flaps, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Hematoma, Postoperative Complications, medicine, Humans, Child, Aged, Retrospective Studies, Aged, 80 and over, business.industry, Head and neck cancer, Retrospective cohort study, General Medicine, Pedicled Flap, Perioperative, Middle Aged, Plastic Surgery Procedures, medicine.disease, Surgery, Otorhinolaryngology, Head and Neck Neoplasms, 030220 oncology & carcinogenesis, Inclusion and exclusion criteria, Female, business, Complication
Abstract: BACKGROUND Head and neck (HN) defects after tumor extirpation can be challenging to repair. Historically, pedicled flaps were the mainstay for reconstruction, but recently, free tissue transfer has been preferred. This study compares patient characteristics and flap outcomes for HN defects over a 30-year period at the authors' institution. METHODS Head and neck cancer patients receiving flap reconstruction from 1983 to 2013 were included. Records were reviewed for demographic and perioperative data. Flap complications were compared and statistical tests were 2-tailed with a significance level of 0.05. RESULTS Eight hundred sixty-one patients fulfilled inclusion and exclusion criteria. Pedicled reconstruction predominated during early time-points (96.3% pedicled), compared with later years (69.5% free-tissue). Free flaps were associated with significantly longer operative times (643.5 versus 429.7 minutes, P
Published: 2017

48. Pharmacologic targeting of S6K1 in PTEN-deficient neoplasia

Author: David R. Plas, Kelli N. Ennis, Pranjal Sarma, Hala Elnakat Thomas, Tony T. Jiang, Hongqi Liu, Xizhi Feng, Liang Niu, Atsuo T. Sasaki, Satoshi Kofuji, Yiwen Stratton, Sang-Oh Yoon, and Catherine A. Behrmann
Subjects: 0301 basic medicine, Leukemia, T-Cell, Pyridones, Aminopyridines, P70-S6 Kinase 1, Antineoplastic Agents, Apoptosis, Biology, Heterocyclic Compounds, 4 or More Rings, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Cell Line, Tumor, Neoplasms, medicine, Cytotoxic T cell, PTEN, Animals, Humans, Kinome, Phosphorylation, lcsh:QH301-705.5, PI3K/AKT/mTOR pathway, TOR Serine-Threonine Kinases, PTEN Phosphohydrolase, Receptor Protein-Tyrosine Kinases, Ribosomal Protein S6 Kinases, 70-kDa, medicine.disease, 3. Good health, Leukemia, 030104 developmental biology, lcsh:Biology (General), 030220 oncology & carcinogenesis, Gene Knockdown Techniques, Cancer research, biology.protein, Glioblastoma, Tyrosine kinase, Signal Transduction
Abstract: Summary: Genetic S6K1 inactivation can induce apoptosis in PTEN-deficient cells. We analyzed the therapeutic potential of S6K1 inhibitors in PTEN-deficient T cell leukemia and glioblastoma. Results revealed that the S6K1 inhibitor LY-2779964 was relatively ineffective as a single agent, while S6K1-targeting AD80 induced cytotoxicity selectively in PTEN-deficient cells. In vivo, AD80 rescued 50% of mice transplanted with PTEN-deficient leukemia cells. Cells surviving LY-2779964 treatment exhibited inhibitor-induced S6K1 phosphorylation due to increased mTOR-S6K1 co-association, which primed the rapid recovery of S6K1 signaling. In contrast, AD80 avoided S6K1 phosphorylation and mTOR co-association, resulting in durable suppression of S6K1-induced signaling and protein synthesis. Kinome analysis revealed that AD80 coordinately inhibits S6K1 together with the TAM family tyrosine kinase AXL. TAM suppression by BMS-777607 or genetic knockdown potentiated cytotoxic responses to LY-2779964 in PTEN-deficient glioblastoma cells. These results reveal that combination targeting of S6K1 and TAMs is a potential strategy for treatment of PTEN-deficient malignancy. : Liu et al. find that the S6K1 inhibitor, AD80, is selectively cytotoxic for PTEN-deficient cancer cells, while LY-2779964 is ineffective as a single agent. AD80 avoids S6K1 priming and co-targets TAM tyrosine kinases. Combining LY-2779964 with the TAM kinase inhibitor BMS-777607 is selectively cytotoxic for PTEN-deficient cells. Keywords: S6K1, leukemia, LY-2779964, PF4708671, AD80, Pten, BMS-777607, AXL, TAM, glioblastoma
Published: 2017

49. Perceiving active listening activates the reward system and improves the impression of relevant experiences

Author: Yukiko Mochizuki, Norihiro Sadato, Kentaro Tokutake, Kazufumi Yoshihara, Akihiro T. Sasaki, Hiroki C. Tanabe, Ryoji Shinohara, Yuka Sugisawa, Hiroaki Kawamichi, Tokie Anme, and Sho K. Sugawara
Subjects: Male, Social Psychology, media_common.quotation_subject, Functional magnetic resonance imaging, Emotions, Development, Affect (psychology), behavioral disciplines and activities, 050105 experimental psychology, Developmental psychology, Young Adult, 03 medical and health sciences, Behavioral Neuroscience, Reward system, 0302 clinical medicine, Reward, Perception, Emotional appraisal, Neural Pathways, Active listening, Image Processing, Computer-Assisted, otorhinolaryngologic diseases, Humans, Interpersonal Relations, 0501 psychology and cognitive sciences, Cooperative Behavior, media_common, Analysis of Variance, Brain Mapping, Neural correlates of consciousness, 05 social sciences, Brain, Articles, Magnetic Resonance Imaging, humanities, Feeling, Mentalization, Auditory Perception, Female, Psychology, Insula, Photic Stimulation, psychological phenomena and processes, 030217 neurology & neurosurgery
Abstract: Although active listening is an influential behavior, which can affect the social responses of others, the neural correlates underlying its perception have remained unclear. Sensing active listening in social interactions is accompanied by an improvement in the recollected impressions of relevant experiences and is thought to arouse positive feelings. We therefore hypothesized that the recognition of active listening activates the reward system, and that the emotional appraisal of experiences that had been subject to active listening would be improved. To test these hypotheses, we conducted functional magnetic resonance imaging (fMRI) on participants viewing assessments of their own personal experiences made by evaluators with or without active listening attitude. Subjects rated evaluators who showed active listening more positively. Furthermore, they rated episodes more positively when they were evaluated by individuals showing active listening. Neural activation in the ventral striatum was enhanced by perceiving active listening, suggesting that this was processed as rewarding. It also activated the right anterior insula, representing positive emotional reappraisal processes. Furthermore, the mentalizing network was activated when participants were being evaluated, irrespective of active listening behavior. Therefore, perceiving active listening appeared to result in positive emotional appraisal and to invoke mental state attribution to the active listener.
Published: 2014
Full Text: View/download PDF

50. RAS/PI3K Crosstalk and Cetuximab Resistance in Head and Neck Squamous Cell Carcinoma

Author: Athina Giagini, Hiroumi Matsuzaki, Theodore Rampias, Andreas Scorilas, Amanda Psyrri, Spiros Siolos, and Clarence T. Sasaki
Subjects: Oncology, Cancer Research, medicine.medical_specialty, MAP Kinase Signaling System, Blotting, Western, Cetuximab, Antineoplastic Agents, Mice, Transgenic, Drug resistance, Biology, Antibodies, Monoclonal, Humanized, Real-Time Polymerase Chain Reaction, Mice, Phosphatidylinositol 3-Kinases, Cyclin D1, Internal medicine, medicine, Animals, Humans, Gene Knock-In Techniques, HRAS, neoplasms, PI3K/AKT/mTOR pathway, Microscopy, Confocal, Reverse Transcriptase Polymerase Chain Reaction, Squamous Cell Carcinoma of Head and Neck, Kinase, Head and neck cancer, Receptor Cross-Talk, medicine.disease, Head and neck squamous-cell carcinoma, digestive system diseases, Drug Resistance, Neoplasm, Head and Neck Neoplasms, Mutation, Carcinoma, Squamous Cell, ras Proteins, Transcriptome, medicine.drug
Abstract: Purpose: Cetuximab, an antibody directed against the EGF receptor, is an effective clinical therapy for patients with head and neck squamous cell cancer (HNSCC). Despite great clinical promise, intrinsic or acquired cetuximab resistance hinders successful treatment outcomes but little is known about the underlying mechanism. Experimental Design: To study the role of oncogenic HRAS in cetuximab resistance in HNSCC, the frequency of oncogenic HRAS mutations was determined in a cohort of 180 genomic DNAs from head and neck cancer specimens. We also used a combination of cetuximab-resistant cell lines and a transgenic mouse model of RAS-driven oral cancer to identify an oncogenic RAS-specific gene expression signature that promotes cetuximab resistance. Results: Here, we show that activation of RAS signaling leads to persistent extracellular signal–regulated kinase 1/2 signaling and consequently to cetuximab resistance. HRAS depletion in cells containing oncogenic HRAS or PIK3CA restored cetuximab sensitivity. In our study, the gene expression signature of c-MYC, BCL-2, BCL-XL, and cyclin D1 upon activation of MAPK signaling was not altered by cetuximab treatment, suggesting that this signature may have a pivotal role in cetuximab resistance of RAS-activated HNSCC. Finally, a subset of patients with head and neck cancer with oncogenic HRAS mutations was found to exhibit de novo resistance to cetuximab-based therapy. Conclusions: Collectively, these findings identify a distinct cetuximab resistance mechanism. Oncogenic HRAS in HNSCC promotes activation of ERK signaling, which in turn mediates cetuximab resistance through a specific gene expression signature. Clin Cancer Res; 20(11); 2933–46. ©2014 AACR.
Published: 2014
Full Text: View/download PDF

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Journal

Database

Publisher

1,430 results on '"T, Sasaki"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources